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2021"]}],"id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007525","name":"Universit\u00e9 de Rennes 1","doi-asserted-by":"publisher","award":["D\u00e9fis scientifiques, 2020"],"award-info":[{"award-number":["D\u00e9fis scientifiques, 2020"]}],"id":[{"id":"10.13039\/501100007525","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    In the\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    zygote, mutations in\n                    <jats:italic>zyg-8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>DCLK1<\/jats:italic>\n                    <\/jats:sup>\n                    , the sole Doublecortin-family member, disrupt mitotic spindle positioning, as seen by immunofluorescence. Doublecortin proteins bind microtubules and are thought to stabilise or rigidify them. In the zygote, ZYG-8 only modestly affects microtubule growth and nucleation. We thus investigated whether these moderate dynamic perturbations alone could explain the spindle mispositioning observed in\n                    <jats:italic>zyg-8<\/jats:italic>\n                    mutants. Using three complementary genetic perturbations\u2014RNAi-mediated depletion of ZYG-8, its overexpression, and the thermosensitive\n                    <jats:italic>zyg-8(or484ts)<\/jats:italic>\n                    mutant (that disrupts microtubule binding)\u2014we observed altered spindle pole oscillations and changes in microtubule cortical-contact behaviour, indicative of impaired cortical forces. Importantly, these phenotypes could not be fully explained by previously reported alterations in microtubule dynamics, suggesting an additional mechanism. Our findings indicate that ZYG-8 increases microtubule rigidity: ZYG-8 depletion or mutation led to more frequent microtubule bending and higher curvature and tortuosity. Simulations confirmed that reduced rigidity prolongs cortical contact lifetimes, an effect we experimentally observed in\n                    <jats:italic>zyg-8(RNAi)<\/jats:italic>\n                    embryos. Using custom biophysical assays, we showed that microtubule softening in\n                    <jats:italic>zyg-8(RNAi)<\/jats:italic>\n                    embryos and\n                    <jats:italic>zyg-8<\/jats:italic>\n                    mutants reduced the efficiency of centring forces, leading to exaggerated spindle-pole oscillations. In mutants, the largest oscillations caused spindle poles to move closer to the cell periphery, preventing re-centring and resulting in spindle mispositioning and misorientation during late anaphase. Importantly, reducing cortical pulling forces rescued orientation defects, highlighting the importance of balanced pulling-pushing forces for proper spindle positioning. We propose that sufficient microtubule rigidity is essential for generating effective cortical pushing forces, potentially in synergy with other microtubule properties, which contribute to centring mechanisms that ensure accurate spindle orientation in late mitosis. Given that DCLK1 is frequently deregulated in human cancers and that accurate spindle positioning is essential for maintaining cell proliferation-differentiation balance, these findings may have implications for understanding how disruptions in microtubule mechanics contribute to carcinogenesis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1012196","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T17:35:31Z","timestamp":1782754531000},"page":"e1012196","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6798-0920","authenticated-orcid":true,"given":"Louis","family":"Cueff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2382-5408","authenticated-orcid":true,"given":"Lo\u00efc","family":"Schmitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4058-2669","authenticated-orcid":true,"given":"Ewen","family":"Huet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0191-8043","authenticated-orcid":true,"given":"Sylvain","family":"Pastezeur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9line","family":"Coquil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7855-920X","authenticated-orcid":true,"given":"Talia","family":"Savary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1132-798X","authenticated-orcid":true,"given":"Anouk","family":"S\u00e9nard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9998-4844","authenticated-orcid":true,"given":"Jacques","family":"P\u00e9cr\u00e9aux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1128-1322","authenticated-orcid":true,"given":"H\u00e9l\u00e8ne","family":"Bouvrais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"issue":"5","key":"pgen.1012196.ref001","doi-asserted-by":"crossref","DOI":"10.1002\/bies.201900244","article-title":"The 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