{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T18:34:52Z","timestamp":1786473292675,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pgen.1012235","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000}}],"reference-count":85,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM119515"],"award-info":[{"award-number":["R35GM119515"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["HD121176"],"award-info":[{"award-number":["HD121176"]}]},{"name":"University of Rochester Messersmith Fellowship"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Meiotic drivers achieve biased transmission to the next generation, often at the expense of their host. Drive is widespread and can shape the evolution of proteins, chromosome structure, and karyotypes. The sperm killer\n                    <jats:italic>Segregation Distorter<\/jats:italic>\n                    (\n                    <jats:italic>SD<\/jats:italic>\n                    ) in\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    is a well-studied driver but like most complex drivers, its mechanism remains elusive.\n                    <jats:italic>SD<\/jats:italic>\n                    is a multigene complex frequently associated with chromosomal inversions where the main driver locus, a truncated duplication of the gene\n                    <jats:italic>RanGAP,<\/jats:italic>\n                    kills wild-type sperm containing a satellite DNA called\n                    <jats:italic>Responder<\/jats:italic>\n                    (\n                    <jats:italic>Rsp<\/jats:italic>\n                    ). Small RNAs are frequently implicated in the mechanisms of sperm killers, and we recently showed that\n                    <jats:italic>Rsp<\/jats:italic>\n                    is a source of small RNAs. Here we study the transcriptomes of two\n                    <jats:italic>SD<\/jats:italic>\n                    haplotypes and our results link\n                    <jats:italic>Rsp<\/jats:italic>\n                    expression and\/or RNAs to drive. We found that\n                    <jats:italic>Rsp<\/jats:italic>\n                    -derived small RNAs are underrepresented in driving testes of only one of the\n                    <jats:italic>SD<\/jats:italic>\n                    haplotypes. We show that over-expressing\n                    <jats:italic>Rsp<\/jats:italic>\n                    is sufficient to reduce drive strength in the haplotype with downregulated\n                    <jats:italic>Rsp<\/jats:italic>\n                    but not the other. We therefore shed light on the mechanism of\n                    <jats:italic>SD<\/jats:italic>\n                    by making a connection between the target and the drive phenotype. Additionally, our data imply that different haplotypes of complex drivers, like\n                    <jats:italic>SD<\/jats:italic>\n                    , can vary in their mechanism.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1012235","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:30:43Z","timestamp":1785778243000},"page":"e1012235","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disruption of small RNAs and mechanistic variation in Segregation Distorter: A sperm-killing drive system in Drosophila melanogaster"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2015-3680","authenticated-orcid":true,"given":"Logan T.","family":"Edvalson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolu","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9361-1190","authenticated-orcid":true,"given":"Ching-Ho","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-5686","authenticated-orcid":true,"given":"Amanda M.","family":"Larracuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"issue":"857","key":"pgen.1012235.ref001","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1086\/281969","article-title":"Meiotic drive as an evolutionary force","volume":"91","author":"L Sandler","year":"1957","journal-title":"Am Nat"},{"key":"pgen.1012235.ref002","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.ge.04.120170.002205","article-title":"Mechanisms of meiotic drive","volume":"4","author":"S Zimmering","year":"1970","journal-title":"Annu Rev Genet"},{"key":"pgen.1012235.ref003","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.tree.2016.02.001","article-title":"The ecology and evolutionary dynamics of meiotic drive","volume":"31","author":"AK Lindholm","year":"2016","journal-title":"Trends Ecol Evol"},{"key":"pgen.1012235.ref004","doi-asserted-by":"crossref","first-page":"10863","DOI":"10.1073\/pnas.1102343108","article-title":"Selfish genetic elements, genetic conflict, and evolutionary innovation","author":"JH Werren","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"pgen.1012235.ref005","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.tree.2009.10.005","article-title":"Genetic conflict and sex chromosome evolution","volume":"25","author":"CD Meiklejohn","year":"2010","journal-title":"Trends Ecol Evol"},{"key":"pgen.1012235.ref006","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1093\/genetics\/105.3.663","article-title":"Virility deficiency and the sex-ratio trait in Drosophila pseudoobscura. II. Multiple mating and overall virility selection","volume":"105","author":"CI Wu","year":"1983","journal-title":"Genetics"},{"issue":"6","key":"pgen.1012235.ref007","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/bies.950130609","article-title":"Why is Mendelian segregation so exact?","volume":"13","author":"JF Crow","year":"1991","journal-title":"Bioessays"},{"issue":"2050","key":"pgen.1012235.ref008","doi-asserted-by":"crossref","first-page":"20250779","DOI":"10.1098\/rspb.2025.0779","article-title":"The metabolic costs of meiotic drive","volume":"292","author":"SL Bradshaw","year":"2025","journal-title":"Proc R Soc Biol Sci"},{"key":"pgen.1012235.ref009","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2019.03.046","article-title":"Fertility costs of meiotic drivers","volume":"29","author":"SE Zanders","year":"2019","journal-title":"Curr Biol"},{"issue":"2","key":"pgen.1012235.ref010","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/0040-5809(75)90012-X","article-title":"Modifier theory and meiotic drive","volume":"7","author":"DL Hartl","year":"1975","journal-title":"Theor Popul Biol"},{"issue":"1913","key":"pgen.1012235.ref011","doi-asserted-by":"crossref","first-page":"20191430","DOI":"10.1098\/rspb.2019.1430","article-title":"Meiotic drive mechanisms: lessons from Drosophila","volume":"286","author":"C Courret","year":"2019","journal-title":"Proc R Soc Biol Sci"},{"issue":"15","key":"pgen.1012235.ref012","doi-asserted-by":"crossref","first-page":"6742","DOI":"10.1128\/MCB.24.15.6742-6750.2004","article-title":"Dissection of a natural RNA silencing process in the Drosophila melanogaster germ line","volume":"24","author":"AA Aravin","year":"2004","journal-title":"Mol Cell Biol"},{"issue":"11","key":"pgen.1012235.ref013","article-title":"A sex-ratio meiotic drive system in Drosophila simulans. I: an autosomal suppressor","volume":"5","author":"Y Tao","year":"2007","journal-title":"PLoS Biol"},{"key":"pgen.1012235.ref014","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.cell.2018.03.009","article-title":"A kinesin-14 motor activates neocentromeres to promote meiotic drive in maize","volume":"173","author":"RK Dawe","year":"2018","journal-title":"Cell"},{"issue":"15","key":"pgen.1012235.ref015","doi-asserted-by":"crossref","first-page":"4110","DOI":"10.1073\/pnas.1519332113","article-title":"Rapid evolution of a Y-chromosome heterochromatin protein underlies sex chromosome meiotic drive","volume":"113","author":"Q Helleu","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"pgen.1012235.ref016","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000094","article-title":"Contingency in the convergent evolution of a regulatory network: Dosage compensation in Drosophila","volume":"17","author":"C Ellison","year":"2019","journal-title":"PLoS Biol"},{"key":"pgen.1012235.ref017","article-title":"Endogenous RNAi silences a burgeoning sex chromosome arms race","author":"J Vedanayagam","year":"2022","journal-title":"bioRxiv"},{"issue":"2010","key":"pgen.1012235.ref018","first-page":"20231823","article-title":"Genetic conflict and the origin of multigene families: implications for sex chromosome evolution","volume":"290","author":"E Mart\u00ed","year":"2023","journal-title":"Proc Biol Sci"},{"issue":"2","key":"pgen.1012235.ref019","doi-asserted-by":"crossref","DOI":"10.1093\/molbev\/msag020","article-title":"The evolutionary genomics of meiotic drive","volume":"43","author":"DC Presgraves","year":"2026","journal-title":"Mol Biol Evol"},{"issue":"2","key":"pgen.1012235.ref020","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/genetics\/44.2.233","article-title":"Meiotic drive in natural populations of Drosophila Melanogaster. I. the cytogenetic basis of segregation-distortion","volume":"44","author":"L Sandler","year":"1959","journal-title":"Genetics"},{"issue":"4","key":"pgen.1012235.ref021","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1093\/genetics\/121.4.765","article-title":"Cytogenetic analysis of segregation distortion in Drosophila melanogaster: the cytological organization of the Responder (Rsp) locus","volume":"121","author":"S Pimpinelli","year":"1989","journal-title":"Genetics"},{"issue":"2","key":"pgen.1012235.ref022","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/0092-8674(88)90550-8","article-title":"Association between a satellite DNA sequence and the Responder of Segregation Distorter in D. melanogaster","volume":"54","author":"CI Wu","year":"1988","journal-title":"Cell"},{"key":"pgen.1012235.ref023","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S1534-5807(01)00042-9","article-title":"Nuclear mislocalization of enzymatically active RanGAP causes segregation distortion in Drosophila","volume":"1","author":"A Kusano","year":"2001","journal-title":"Developmental Cell"},{"issue":"5408","key":"pgen.1012235.ref024","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1126\/science.283.5408.1742","article-title":"Truncated RanGAP encoded by the Segregation Distorter locus of Drosophila","volume":"283","author":"C Merrill","year":"1999","journal-title":"Science"},{"issue":"14","key":"pgen.1012235.ref025","doi-asserted-by":"crossref","DOI":"10.1016\/S0960-9822(02)00970-3","article-title":"The Ran GTPase: theme and variations","volume":"12","author":"M Dasso","year":"2002","journal-title":"Curr Biol"},{"key":"pgen.1012235.ref026","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/genetics\/86.2.321","article-title":"On the components of segregation distortion in Drosophila melanogaster","volume":"86","author":"B Ganetzky","year":"1977","journal-title":"Genetics"},{"issue":"3","key":"pgen.1012235.ref027","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1093\/genetics\/95.3.693","article-title":"A modified model of segregation distortion in Drosophila Melanogaster","volume":"95","author":"Y Hiraizumi","year":"1980","journal-title":"Genetics"},{"key":"pgen.1012235.ref028","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1093\/genetics\/45.9.1269","article-title":"Meiotic drive in natural populations of Drosophila melanogaster. IV. Instability at the Segregation-Distorter locus","volume":"45","author":"L Sandler","year":"1960","journal-title":"Genetics"},{"issue":"1","key":"pgen.1012235.ref029","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1534\/genetics.112.141390","article-title":"The selfish Segregation Distorter gene complex of Drosophila melanogaster","volume":"192","author":"AM Larracuente","year":"2012","journal-title":"Genetics"},{"issue":"7","key":"pgen.1012235.ref030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1009662","article-title":"Distinct spermiogenic phenotypes underlie sperm elimination in the Segregation Distorter meiotic drive system","volume":"17","author":"M Herbette","year":"2021","journal-title":"PLoS Genet"},{"key":"pgen.1012235.ref031","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/S0022-5320(77)80011-7","article-title":"Dynamics of spermiogenesis in Drosophila melanogaster. VII. Effects of segregation distorter (SD) chromosome","volume":"58","author":"KT Tokuyasu","year":"1977","journal-title":"J Ultrastruct Res"},{"issue":"1","key":"pgen.1012235.ref032","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1093\/genetics\/101.1.57","article-title":"Defective Histone Transition during Spermiogenesis in Heterozygous Segregation Distorter males of Drosophila melanogaster","volume":"101","author":"E Hauschteck-Jungen","year":"1982","journal-title":"Genetics"},{"key":"pgen.1012235.ref033","article-title":"Pervasive suppressors halt the spread of selfish Segregation Distorter in a natural population","author":"C-H Chang","year":"2026","journal-title":"Genetics"},{"key":"pgen.1012235.ref034","article-title":"Heterochromatin-dependent transcription of satellite DNAs in the Drosophila melanogaster female germline","volume":"10","author":"X Wei","year":"2021","journal-title":"eLife"},{"issue":"9","key":"pgen.1012235.ref035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1009591","article-title":"RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility","volume":"17","author":"P Chen","year":"2021","journal-title":"PLoS Genet"},{"key":"pgen.1012235.ref036","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.48940","article-title":"RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster","volume":"8","author":"WK Mills","year":"2019","journal-title":"eLife"},{"issue":"1","key":"pgen.1012235.ref037","doi-asserted-by":"crossref","first-page":"5701","DOI":"10.1038\/s41467-026-72480-y","article-title":"Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive in Drosophila","volume":"17","author":"T Kumon","year":"2026","journal-title":"Nat Commun"},{"issue":"46","key":"pgen.1012235.ref038","doi-asserted-by":"crossref","first-page":"16460","DOI":"10.1073\/pnas.1410534111","article-title":"siRNAs from an X-linked satellite repeat promote X-chromosome recognition in Drosophila melanogaster","volume":"111","author":"DU Menon","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"pgen.1012235.ref039","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1101\/gad.209841.112","article-title":"Piwi induces piRNA-guided transcriptional silencing and establishment of a repressive chromatin state","volume":"27","author":"A Le Thomas","year":"2013","journal-title":"Genes Dev"},{"issue":"3","key":"pgen.1012235.ref040","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1534\/genetics.112.147561","article-title":"Mutations to the piRNA pathway component aubergine enhance meiotic drive of segregation distorter in Drosophila melanogaster","volume":"193","author":"SL Gell","year":"2013","journal-title":"Genetics"},{"issue":"4","key":"pgen.1012235.ref041","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.tibs.2015.12.008","article-title":"One Loop to Rule Them All: The Ping-Pong Cycle and piRNA-Guided Silencing","volume":"41","author":"B Czech","year":"2016","journal-title":"Trends Biochem Sci"},{"key":"pgen.1012235.ref042","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1101\/gad.345041.120","article-title":"piRNA-mediated gene regulation and adaptation to sex-specific transposon expression in D. melanogaster male germline","volume":"35","author":"P Chen","year":"2021","journal-title":"Genes Dev"},{"key":"pgen.1012235.ref043","article-title":"PANGEA: a new gene set enrichment tool for Drosophila and common research organisms","volume":"51","author":"Y Hu","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"pgen.1012235.ref044","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkt1102","article-title":"The Reactome pathway knowledgebase","volume":"42","author":"D Croft","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"pgen.1012235.ref045","doi-asserted-by":"crossref","DOI":"10.1080\/21565562.2015.1041345","article-title":"Separating from the pack: molecular mechanisms of Drosophila spermatid individualization","volume":"5","author":"J Steinhauer","year":"2015","journal-title":"Spermatogenesis"},{"key":"pgen.1012235.ref046","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"P Langfelder","year":"2008","journal-title":"BMC Bioinformatics"},{"issue":"3","key":"pgen.1012235.ref047","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1093\/genetics\/58.3.399","article-title":"Temperature sensitivity of segregation-distortion in Drosophila melanogaster","volume":"58","author":"EJ Mange","year":"1968","journal-title":"Genetics"},{"key":"pgen.1012235.ref048","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.80067","article-title":"The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters","volume":"11","author":"L Baumgartner","year":"2022","journal-title":"Elife"},{"issue":"9","key":"pgen.1012235.ref049","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1016\/j.cub.2023.03.057","article-title":"Genetic control of a sex-specific piRNA program","volume":"33","author":"P Chen","year":"2023","journal-title":"Curr Biol"},{"key":"pgen.1012235.ref050","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.93194","article-title":"Evolutionary adaptation of an HP1-protein chromodomain integrates chromatin and DNA sequence signals","volume":"13","author":"L Baumgartner","year":"2024","journal-title":"Elife"},{"key":"pgen.1012235.ref051","article-title":"Targeted editing of pericentromeric satellite DNA alters sensitivity to meiotic drive","author":"DG Eickbush","year":"2026","journal-title":"bioRxiv"},{"key":"pgen.1012235.ref052","article-title":"Testing the function of widespread transcription of the testis","author":"T Kumon","year":"2025","journal-title":"bioRxiv"},{"issue":"2","key":"pgen.1012235.ref053","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2418541122","article-title":"Escalation of genome defense capacity enables control of an expanding meiotic driver","volume":"122","author":"P Chen","year":"2025","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6239","key":"pgen.1012235.ref054","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/341248a0","article-title":"Fitness reduction associated with the deletion of a satellite DNA array","volume":"341","author":"CI Wu","year":"1989","journal-title":"Nature"},{"key":"pgen.1012235.ref055","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1242\/jcs.004663","article-title":"Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila","volume":"120","author":"C Rathke","year":"2007","journal-title":"J Cell Sci"},{"key":"pgen.1012235.ref056","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.89373","article-title":"Chromosome-specific maturation of the epigenome in the Drosophila male germline","volume":"12","author":"JT Anderson","year":"2023","journal-title":"Elife"},{"issue":"17","key":"pgen.1012235.ref057","doi-asserted-by":"crossref","DOI":"10.1016\/j.devcel.2023.06.010","article-title":"Localization of the Drosophila pioneer factor GAF to subnuclear foci is driven by DNA binding and required to silence satellite repeat expression","volume":"58","author":"MM Gaskill","year":"2023","journal-title":"Dev Cell"},{"key":"pgen.1012235.ref058","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.78981","article-title":"Epistatic selection on a selfish Segregation Distorter supergene - drive, recombination, and genetic load","volume":"11","author":"B Navarro-Dominguez","year":"2022","journal-title":"Elife"},{"issue":"5","key":"pgen.1012235.ref059","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1111\/evo.12658","article-title":"Origin, evolution, and population genetics of the selfish Segregation Distorter gene duplication in European and African populations of Drosophila melanogaster","volume":"69","author":"CL Brand","year":"2015","journal-title":"Evolution"},{"issue":"5","key":"pgen.1012235.ref060","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1000463","article-title":"Large-scale selective sweep among Segregation Distorter chromosomes in African populations of Drosophila melanogaster","volume":"5","author":"DC Presgraves","year":"2009","journal-title":"PLoS Genet"},{"issue":"3","key":"pgen.1012235.ref061","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1093\/genetics\/107.3.375","article-title":"Factors Influencing the Effect of Segregation Distortion in Natural Populations of Drosophila melanogaster","volume":"107","author":"RG Temin","year":"1984","journal-title":"Genetics"},{"issue":"18","key":"pgen.1012235.ref062","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1016\/j.cub.2024.07.079","article-title":"Mechanoresponsive regulation of myogenesis by the force-sensing transcriptional regulator Tono","volume":"34","author":"X Zhang","year":"2024","journal-title":"Curr Biol"},{"issue":"6584","key":"pgen.1012235.ref063","article-title":"Fly cell atlas: a single-nucleus transcriptomic atlas of the adult fruit fly","volume":"375","author":"H Li","year":"2022","journal-title":"Science"},{"issue":"4","key":"pgen.1012235.ref064","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1038\/s41559-018-0471-0","article-title":"Gene duplicates resolving sexual conflict rapidly evolved essential gametogenesis functions","volume":"2","author":"NW VanKuren","year":"2018","journal-title":"Nat Ecol Evol"},{"key":"pgen.1012235.ref065","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/BF00335253","article-title":"Dynamics of spermiogenesis in Drosophila melanogaster","volume":"124","author":"KT Tokuyasu","year":"1972","journal-title":"Z Zellforsch"},{"issue":"1","key":"pgen.1012235.ref066","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1038\/s41467-025-68254-7","article-title":"Selfish chromosomes exploit a germline checkpoint to eliminate competing gametes","volume":"17","author":"JT Ridges","year":"2026","journal-title":"Nat Commun"},{"key":"pgen.1012235.ref067","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1093\/genetics\/149.1.143","article-title":"Male sterility and meiotic drive associated with sex chromosome rearrangements in Drosophila: role of X-Y pairing","volume":"149","author":"BD McKee","year":"1998","journal-title":"Genetics"},{"issue":"2","key":"pgen.1012235.ref068","article-title":"Chk2 and p53 regulate the transmission of healed chromosomes in the Drosophila male germline","volume":"10","author":"SWA Titen","year":"2014","journal-title":"PLoS Genet"},{"issue":"6","key":"pgen.1012235.ref069","doi-asserted-by":"crossref","DOI":"10.1093\/g3journal\/jkaf076","article-title":"Consequences of drive on the evolution of genomes and species","volume":"15","author":"LT Edvalson","year":"2025","journal-title":"G3 (Bethesda)"},{"key":"pgen.1012235.ref070","doi-asserted-by":"crossref","first-page":"102111","DOI":"10.1016\/j.gde.2023.102111","article-title":"New perspectives on the causes and consequences of male meiotic drive","volume":"83","author":"C Courret","year":"2023","journal-title":"Curr Opin Genet Dev"},{"issue":"3","key":"pgen.1012235.ref071","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1093\/genetics\/137.3.803","article-title":"Genetic analysis of the brahma gene of Drosophila melanogaster and polytene chromosome subdivisions 72AB","volume":"137","author":"BJ Brizuela","year":"1994","journal-title":"Genetics"},{"key":"pgen.1012235.ref072","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1093\/genetics\/107.3.423","article-title":"On the components of segregation distortion in Drosophila melanogaster. III. Nature of the enhancer of SD","volume":"107","author":"JG Brittnacher","year":"1984","journal-title":"Genetics"},{"issue":"29","key":"pgen.1012235.ref073","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1405500111","article-title":"Optimized CRISPR\/Cas tools for efficient germline and somatic genome engineering in Drosophila","volume":"111","author":"F Port","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pgen.1012235.ref074","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1534\/genetics.118.301765","article-title":"Heterochromatin-enriched assemblies reveal the sequence and organization of the Drosophila melanogaster Y chromosome","volume":"211","author":"CH Chang","year":"2019","journal-title":"Genetics"},{"key":"pgen.1012235.ref075","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-1-59745-583-1_9","article-title":"Methods for Homologous Recombination in Drosophila.","volume-title":"Drosophila: Methods and Protocols","author":"KA Maggert","year":"2008"},{"issue":"4","key":"pgen.1012235.ref076","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"10","key":"pgen.1012235.ref077","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000234","article-title":"Species-specific heterochromatin prevents mitotic chromosome segregation to cause hybrid lethality in Drosophila","volume":"7","author":"PM Ferree","year":"2009","journal-title":"PLoS Biol"},{"key":"pgen.1012235.ref078","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/genetics\/129.1.133","article-title":"On the components of segregation distortion in Drosophila melanogaster. V. Molecular analysis of the Sd locus","volume":"129","author":"PA Powers","year":"1991","journal-title":"Genetics"},{"issue":"5","key":"pgen.1012235.ref079","doi-asserted-by":"crossref","first-page":"269","DOI":"10.2144\/000114102","article-title":"Terminator oligo blocking efficiently eliminates rRNA from Drosophila small RNA sequencing libraries","volume":"55","author":"ML Wickersheim","year":"2013","journal-title":"Biotechniques"},{"issue":"4","key":"pgen.1012235.ref080","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nat Methods"},{"issue":"1","key":"pgen.1012235.ref081","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"A Dobin","year":"2013","journal-title":"Bioinformatics"},{"issue":"2","key":"pgen.1012235.ref082","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq--a Python framework to work with high-throughput sequencing data","volume":"31","author":"S Anders","year":"2015","journal-title":"Bioinformatics"},{"issue":"12","key":"pgen.1012235.ref083","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome Biol"},{"issue":"3","key":"pgen.1012235.ref084","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"B Langmead","year":"2009","journal-title":"Genome Biol"},{"key":"pgen.1012235.ref085","volume-title":"Dataset for: Disruption of small RNAs and mechanistic variation in Segregation Distorter--a sperm killing drive system in Drosophila melanogaster","author":"L Edvalson","year":"2026"}],"updated-by":[{"DOI":"10.1371\/journal.pgen.1012235","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000}}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1012235","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T17:46:35Z","timestamp":1786470395000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1012235"}},"subtitle":[],"editor":[{"given":"Sarah E","family":"Zanders","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":85,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1012235","relation":{},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]}}}