{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T03:05:52Z","timestamp":1775185552553,"version":"3.50.1"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1132929"],"award-info":[{"award-number":["OPP1132929"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"},{"name":"Wits Reproductive Health and HIV Institute - A division of Wits Health Consortium (Pty) Ltd and PATH"}],"content-domain":{"domain":["www.plosglobalpublichealth.org"],"crossmark-restriction":false},"short-container-title":["PLOS Glob Public Health"],"abstract":"<jats:p>HIV self-testing (HIVST) is an effective approach to increase testing uptake. While oral fluid-based HIVST has been rapidly scaled, use of blood-based HIVST remains limited. We evaluated the acceptability, feasibility, and accuracy of blood-based HIVST among lay users in Ho Chi Minh City (HCMC), Vietnam. We conducted a cross-sectional study among HIV testing clients at the HCMC Pasteur Institute from March 2019 to October 2020. Participants received one HIVST kit and performed the test in front of an observer. The observer used product-specific questionnaires to collect information on the HIVST process, test results, experiences. The participants\u2019 interpretations of HIVST results were compared to health staff\u2019s interpretations and gold standard laboratory EIA reference tests. Of 2,399 participants who accepted HIVST, 64.7% were men, 62.1% aged 25\u201349 years, 53.5% had a higher education level, 41.4% were employed, and 35.6% were first-time testers. The vast majority (94.4%) desired to use the test in the future, and 93.9% reported willingness to recommend the test. The majority (90.8%) of participants successfully completed the self-test. One factor associated with successful completion was higher education level (aOR = 1.85; 95% CI: 1.32\u20132.61); while participants self-testing with SURE CHECK (aOR = 0.21; 95% CI: 0.12\u20130.37), INSTI (aOR = 0.23; 95% CI: 0.13\u20130.39), and BioSURE (aOR = 0.29; 95% CI: 0.17\u20130.51) or being unemployed, retired, or doing housework (aOR = 0.45; 95% CI: 0.25\u20130.82) were less likely to perform the test successfully. Agreement of positive and negative HIVST results as interpreted by participants and health staff was high (98.1% and 99.9%, respectively). Sensitivity and specificity of the evaluated HIVST were 96.43% (95% CI: 93.62\u201399.23) and 99.9% (95% CI: 99.75\u2013100), respectively. Our findings confirm that blood-based HIVST is highly acceptable, feasible, and accurate. This evidence informs scale-up of HIVST to increase uptake of essential HIV prevention and treatment services.<\/jats:p>","DOI":"10.1371\/journal.pgph.0001438","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T18:45:54Z","timestamp":1675277154000},"page":"e0001438","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgph.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Acceptability, feasibility, and accuracy of blood-based HIV self-testing: A cross-sectional study in Ho Chi Minh City, Vietnam"],"prefix":"10.1371","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5529-5411","authenticated-orcid":true,"given":"Bao Vu","family":"Ngoc","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0302-5353","authenticated-orcid":true,"given":"Mohammed","family":"Majam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4473-878X","authenticated-orcid":true,"given":"Ton","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minh Tran","family":"Hung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anh Luong","family":"Que","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diep Bui","family":"Ngoc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuong Hoang","family":"Le Duy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"pgph.0001438.ref001","volume-title":"Consolidated guidelines on HIV prevention, testing, treatment, service delivery and monitoring: recommendations for public health approach","author":"World Health Organization","year":"2021"},{"key":"pgph.0001438.ref002","author":"World Health Organization and Human Reproduction Programme","year":"2021"},{"key":"pgph.0001438.ref003","unstructured":"Unitaid. 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OraSure Technologies to Drive Accelerated Adoption of OraQuick HIV Self-Test. Intrado GlobeNewswire; 2017 June 27. Available from: https:\/\/www.globenewswire.com\/news-release\/2017\/06\/27\/1029393\/0\/en\/OraSure-Technologies-to-Drive-Accelerated-Adoption-of-OraQuick-HIV-Self-Test.html."},{"key":"pgph.0001438.ref056","unstructured":"Unitaid. Access to HIV self-tests significantly expanded and costs halved thanks to Unitaid agreement. 28 April 2021. Press Release. 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