{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:04:25Z","timestamp":1792033465653,"version":"4.4.0"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T00:00:00Z","timestamp":1692057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"African Centre of Excellence in Infectious Diseases of Humans and Animals"}],"content-domain":{"domain":["www.plosglobalpublichealth.org"],"crossmark-restriction":false},"short-container-title":["PLOS Glob Public Health"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Introduction<\/jats:title><jats:p>Assessing the knowledge and practices of healthcare workers regarding<jats:italic>Toxoplasma gondii<\/jats:italic>infection, diagnosis, treatment, and control is crucial for developing an effective management strategy.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methods<\/jats:title><jats:p>A cross-sectional study was conducted among 175 healthcare workers at Ndola Teaching Hospital and Namwala District Hospital in Zambia from September 2021 to April 2022.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Results<\/jats:title><jats:p>More than half (57.1%) of the respondents were males. Overall, 46(26.3%) and 68 (38.9%) respondents reported good knowledge and practices, respectively. Respondents with a higher number of years of experience (AOR = 0.86, 95% CI: 0.77\u20130.97), who were nurses than clinicians (AOR = 0.17, 95% CI: 0.007\u20130.41) and working at Ndola teaching hospital than Namwala hospital (AOR = 0.34, 95% CI:0.13\u20130.89) were less likely to have good knowledge. Respondents with a degree qualification than a diploma (AOR = 3.04, 95% CI: 1.09\u20138.47) were more likely to have good knowledge. Respondents from Ndola teaching hospital than Namwala hospital (AOR = 0.40, 95% CI: 0.17\u20130.92) were less likely to have good practices.<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Conclusion<\/jats:title><jats:p>Our study revealed that healthcare workers had low levels of knowledge and poor practices, which could have negative implications for the management of toxoplasmosis. To improve their knowledge and practices, continuous medical education in<jats:italic>Toxoplasma<\/jats:italic>related aspects is recommended for in-service healthcare workers.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pgph.0002235","type":"journal-article","created":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T17:21:31Z","timestamp":1692120091000},"page":"e0002235","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgph.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Knowledge and practices of toxoplasmosis among healthcare workers at two large referral hospitals in Zambia: Implications on the One Health Approach"],"prefix":"10.1371","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9490-5609","authenticated-orcid":true,"given":"Victor","family":"Daka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6079-0564","authenticated-orcid":true,"given":"Moses","family":"Mukosha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott K.","family":"Matafwali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steward","family":"Mudenda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8924-193X","authenticated-orcid":true,"given":"Andrew M.","family":"Phiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,8,15]]},"reference":[{"issue":"2","key":"pgph.0002235.ref001","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1080\/20477724.2016.1173325","article-title":"Toxoplasmosis awareness, seroprevalence and risk behavior among pregnant women in the Gampaha district, Sri Lanka","volume":"110","author":"N Chandrasena","year":"2016","journal-title":"Pathog Glob Health"},{"issue":"2","key":"pgph.0002235.ref002","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens11020183","article-title":"Toxoplasma gondii Infections in Animals and Humans in Southern Africa: A Systematic Review and Meta-Analysis","volume":"11","author":"AO Omonijo","year":"2022","journal-title":"Pathogens"},{"key":"pgph.0002235.ref003","article-title":"Toxoplasmosis in South Africa-Old Disease in A New Context","volume":"4","author":"K Hammond-Aryee","year":"2014","journal-title":"Journal of Natural Sciences Research"},{"issue":"11","key":"pgph.0002235.ref004","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph14111413","article-title":"Knowledge and practices of toxoplasmosis among clinical laboratory professionals: A cross-sectional study in Durango, Mexico","volume":"14","author":"C Alvarado-Esquivel","year":"2017","journal-title":"Int J Environ Res Public Health"},{"issue":"3","key":"pgph.0002235.ref005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0090203","article-title":"Toxoplasmosis\u2014A global threat. 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