{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T19:11:50Z","timestamp":1785352310757,"version":"3.55.0"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosglobalpublichealth.org"],"crossmark-restriction":false},"short-container-title":["PLOS Glob Public Health"],"abstract":"<jats:p>Primary dysmenorrhea (PD) is prevalent among adolescent girls but understudied in rural Pakistan. This study examines the prevalence of PD and its associated factors\u00a0among\u00a0girls aged 10\u201319 in a rural district of Pakistan. A cross-sectional survey was conducted in the district of Tando Mohammad Khan (TMK)\u00a0among post-menarchal\u00a0girls aged 10\u201319 using a multistage cluster random sampling. Analyses were conducted using STATA 15.0, with multivariable logistic regression adjusted for survey weights. Among 405 participants, 60.4% (95% CI [54\u201366]) reported PD. Higher body mass index was associated with reduced odds (overweight\/obese: AOR\u2009=\u20090.3, 95% CI [0.1\u20130.9]), while lifestyle factors like physical inactivity (AOR\u2009=\u20091.8, 95% CI [1.2\u20132.9]) and inadequate sleep (AOR\u2009=\u20092.4, 95% CI [1.2\u20133.8]), increased PD odds. Dietary intake data suggested that each 10 g increase in carbohydrate slightly raised (AOR\u2009=\u20091.0, 95% CI [0.9\u20131.1]), whereas every 1 mg increase in vitamin B6 reduced PD odds (AOR\u2009=\u20090.9, 95% CI [0.8\u20130.9]). Menstrual hygiene practices like changing pads less frequently than 6 hours increased PD odds (AOR\u2009=\u20092.1, 95% CI [1.3\u20133.4]), while cloth pad reuse was associated with lower odds (AOR\u2009=\u20090.6, 95% CI [0.4\u20131.0]). PD prevalence is high among adolescents in TMK, with higher BMI linked to reduced PD odds, while diet, lifestyle, and menstrual hygiene significantly influence menstrual health. Further research is needed to guide targeted interventions for adolescent girls\u2019 well-being and to promote gender equity.<\/jats:p>","DOI":"10.1371\/journal.pgph.0006162","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:41:26Z","timestamp":1774546886000},"page":"e0006162","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgph.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prevalence of primary dysmenorrhea and its associated factors among adolescent girls in a rural setting of Pakistan"],"prefix":"10.1371","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2070-8878","authenticated-orcid":true,"given":"Naureen","family":"Rehman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muzna","family":"Hashmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjumand","family":"Rizvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Sajid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Unab","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleema","family":"Gulzar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran Ahmed","family":"Chauhadry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2966-7162","authenticated-orcid":true,"given":"Jai K.","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"issue":"1","key":"pgph.0006162.ref001","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1515\/ijamh-2022-0018","article-title":"Teenage menstrual dysfunction: an overview","volume":"35","author":"M Zehravi","year":"2022","journal-title":"Int J Adolesc Med Health"},{"key":"pgph.0006162.ref002","article-title":"Prevalence, perceptions and effects of dysmenorrhea in school going female adolescents of Karachi, Pakistan","author":"S 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Food composition table for Bangladesh. 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Sensitivity and pain detection thresholds for electrical stimulation after surgery-induced weight loss, and comparison with a nonobese population","volume":"29","author":"P Dodet","year":"2013","journal-title":"Clin J Pain"},{"issue":"5","key":"pgph.0006162.ref039","doi-asserted-by":"crossref","DOI":"10.18203\/2320-1770.ijrcog20231232","article-title":"Study of menstrual disorders and its correlation with BMI in adolescents","volume":"12","author":"M Agrawal","year":"2023","journal-title":"Int J Reprod Contracept Obstet Gynecol"},{"issue":"9","key":"pgph.0006162.ref040","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.3390\/healthcare11091289","article-title":"Severity of Menstrual Pain Is Associated with Nutritional Intake and Lifestyle Habits","volume":"11","author":"Y Naraoka","year":"2023","journal-title":"Healthcare (Basel)"},{"issue":"3","key":"pgph.0006162.ref041","article-title":"Menstrual profile and body mass index among female university students","volume":"7","author":"MA Ghareeb","year":"2019","journal-title":"American Journal of Nursing Research"},{"key":"pgph.0006162.ref042","doi-asserted-by":"crossref","DOI":"10.3390\/nu16111629","article-title":"Obesity, Dietary Patterns, and Hormonal Balance Modulation: Gender-Specific Impacts","volume":"16","author":"E Mazza","year":"2024","journal-title":"Nutrients"},{"issue":"2","key":"pgph.0006162.ref043","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3390\/nu8020068","article-title":"B Vitamins and the Brain: Mechanisms, Dose and Efficacy--A Review","volume":"8","author":"DO Kennedy","year":"2016","journal-title":"Nutrients"},{"key":"pgph.0006162.ref044","unstructured":"Agus JWK, Yudiana PIG, Intan DP, Yudiana PIG, Eka FNP. Physical activity and meditation to reduce primary dysmenorrhea in adolescent. 2017."},{"issue":"1","key":"pgph.0006162.ref045","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1186\/s12887-022-03639-x","article-title":"Are health promoting lifestyles associated with pain intensity and menstrual distress among Iranian adolescent girls?","volume":"22","author":"E Cholbeigi","year":"2022","journal-title":"BMC Pediatr"},{"key":"pgph.0006162.ref046","article-title":"Association of insomnia and academic stress with the onset of dysmenorrhea among females of Lahore, Pakistan","author":"Y Iqra","year":"2022","journal-title":"ScienceOpen Preprints"},{"issue":"5","key":"pgph.0006162.ref047","article-title":"Drivers of malnutrition among late adolescent and young women in rural Pakistan: a cross-sectional assessment of the MaPPS trial","volume":"13","author":"J-AB Baxter","year":"2023","journal-title":"BMJ Open"},{"key":"pgph.0006162.ref048","doi-asserted-by":"crossref","first-page":"1121515","DOI":"10.3389\/frph.2023.1121515","article-title":"Dysmenorrhea in adolescents requires careful investigation of endometriosis-an analysis of early menstrual experiences in a large case-control study","volume":"5","author":"S El-Hadad","year":"2023","journal-title":"Front Reprod Health"},{"issue":"8","key":"pgph.0006162.ref049","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1007\/s00787-023-02345-y","article-title":"Longitudinal associations of menstrual characteristics with mental health problems among Chinese girls","volume":"33","author":"D Yuan","year":"2024","journal-title":"Eur Child Adolesc Psychiatry"},{"issue":"1","key":"pgph.0006162.ref050","doi-asserted-by":"crossref","first-page":"14","DOI":"10.21106\/ijtmrph.27","article-title":"Sociodemographic determinants of early, normal, and delayed menarche among female college-aged students in bangladesh","volume":"1","author":"MSTS Khatun","year":"2017","journal-title":"Int J Transl Med Res Public Health"},{"issue":"1","key":"pgph.0006162.ref051","doi-asserted-by":"crossref","first-page":"140","DOI":"10.4103\/TJOG.TJOG_109_19","article-title":"Correlation between primary dysmenorrhea characteristics, age at menarche, anthropometric variables, gynecological history, management attitudes, and quality of life among undergraduates in Nigeria","volume":"37","author":"G Akunna","year":"2020","journal-title":"Trop J Obstet Gynaecol"},{"key":"pgph.0006162.ref052","unstructured":"Thakre SB, Thakre SS, Reddy M, Rathi N, Pathak K, Ughade S. 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