{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:18:36Z","timestamp":1787681916436,"version":"build-2784847793"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"DZIF","doi-asserted-by":"crossref","award":["TTU 03.709"],"award-info":[{"award-number":["TTU 03.709"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001713","name":"European and Developing Countries Clinical Trials Partnership","doi-asserted-by":"publisher","award":["101145698"],"award-info":[{"award-number":["101145698"]}],"id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosglobalpublichealth.org"],"crossmark-restriction":false},"short-container-title":["PLOS Glob Public Health"],"abstract":"<jats:p>\n                    This cross-sectional study investigates the spectrum and proportion of multidrug-resistant (MDR) bacteria isolated from clinical samples processed at the regional microbiology laboratory of Lambar\u00e9n\u00e9, Gabon. Bacteriological results from 2017 to 2023 were retrospectively analysed to assess the rates of non-susceptibility among the most common isolates from urine, blood, stool, and swabs (wound, urethral, ear, and throat). Antimicrobial susceptibility testing and interpretation were performed according to Clinical Laboratory Standards Institute (CLSI) guidelines.A total of 5,401 cultures were reviewed, with 1,050 yielding clinically relevant bacterial isolates. The most frequently identified pathogens were\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (273\/1050; 26%),\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (192\/1050; 18%), \u03b2-hemolytic\n                    <jats:italic>Streptococci<\/jats:italic>\n                    (160\/1050; 15%), and\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (69\/1050; 6.6%). Urine samples predominantly yielded\n                    <jats:italic>E. coli<\/jats:italic>\n                    and\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , while\n                    <jats:italic>S. aureus<\/jats:italic>\n                    was most common in wound and blood cultures. Overall, 23% (242\/1050) of isolates were classified as MDR, with\n                    <jats:italic>E. coli<\/jats:italic>\n                    accounting for 40% (96\/1050). The proportion of ESBL producers among Enterobacterales was 10% (55\/522). Resistance to third-generation cephalosporins was notably high, especially among\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    (28\/69; 41%) and\n                    <jats:italic>E. coli<\/jats:italic>\n                    (34\/273; 13%). Methicillin-resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (MRSA) represented 12% (22\/192) of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates; 5.5% (58\/1050) of isolates belong to the WHO critical-priority pathogen group.Clinical microbiology data from Lambar\u00e9n\u00e9 indicate that urinary infections are mainly associated with\n                    <jats:italic>E. coli<\/jats:italic>\n                    , whereas\n                    <jats:italic>S. aureus<\/jats:italic>\n                    predominates in wound swabs and blood cultures. The detection of WHO critical-priority pathogens in this setting supports the need for sustained microbiological monitoring and locally adapted measures to improve antibiotic use and infection prevention.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgph.0006705","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T17:23:17Z","timestamp":1787678597000},"page":"e0006705","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgph.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial spectrum and proportion of multidrug-resistant bacteria in clinical samples from a regional hospital in Lambar\u00e9n\u00e9, Gabon (2017\u20132023): Implications for antimicrobial resistance surveillance and public health"],"prefix":"10.1371","volume":"6","author":[{"given":"Maradona Daouda","family":"Agbanrin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4208-6212","authenticated-orcid":true,"given":"Bayode Romeo","family":"Adegbite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8680-2332","authenticated-orcid":true,"given":"Christiane Sidonie","family":"Gouleu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam O\u2019neilla Oye","family":"Bingono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Ulrich","family":"Muandze-Nzambe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustin B.","family":"Boueya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea Dane","family":"Damo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubin M. 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