{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:04:26Z","timestamp":1787954666166,"version":"build-2784847793"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T00:00:00Z","timestamp":1202860800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0000165","type":"journal-article","created":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T00:27:01Z","timestamp":1202862421000},"page":"e165","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Bioinformatics and Functional Analysis of an Entamoeba histolytica Mannosyltransferase Necessary for Parasite Complement Resistance and Hepatical Infection"],"prefix":"10.1371","volume":"2","author":[{"given":"Christian","family":"Weber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Blazquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Marion","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Ausseur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Vats","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mickael","family":"Krzeminski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Christine","family":"Rigothier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachid C.","family":"Maroun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Guill\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2008,2,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"45R","DOI":"10.1093\/glycob\/11.4.45R","article-title":"Glycoconjugate structures of parasitic protozoa.","volume":"11","author":"A Guha-Niyogi","year":"2001","journal-title":"Glycobiology."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"10336","DOI":"10.1073\/pnas.180230697","article-title":"Critical roles of glycosylphosphatidylinositol for <italic>Trypanosoma brucei<\/italic>.","volume":"97","author":"K Nagamune","year":"2000","journal-title":"Proc Natl Acad Sci U S A."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nature00937","article-title":"Synthetic GPI as a candidate anti-toxic vaccine in a model of malaria.","volume":"418","author":"L Schofield","year":"2002","journal-title":"Nature."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1086\/367897","article-title":"Immunoglobulin G (IgG) responses to <italic>Plasmodium falciparum<\/italic> glycosylphosphatidylinositols are short-lived and predominantly of the IgG3 subclass.","volume":"187","author":"CS Boutlis","year":"2003","journal-title":"J Infect Dis."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1093\/emboj\/19.7.1476","article-title":"Highly purified glycosylphosphatidylinositols from <italic>Trypanosoma cruzi<\/italic> are potent proinflammatory agents.","volume":"19","author":"IC Almeida","year":"2000","journal-title":"Embo J."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev.micro.56.012302.160959","article-title":"The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite <italic>Entamoeba histolytica<\/italic>.","volume":"56","author":"WA Petri Jr","year":"2002","journal-title":"Annu Rev Microbiol."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1017\/S0031182096008396","article-title":"Virulent and avirulent <italic>Entamoeba histolytica<\/italic> and <italic>E. dispar<\/italic> differ in their cell surface phosphorylated glycolipids.","volume":"114 ( Pt 2)","author":"S Moody","year":"1997","journal-title":"Parasitology."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1006\/jmbi.2000.3577","article-title":"The major surface antigens of <italic>Entamoeba histolytica<\/italic> trophozoites are GPI-anchored proteophosphoglycans.","volume":"297","author":"S Moody-Haupt","year":"2000","journal-title":"J Mol Biol."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.molbiopara.2005.09.009","article-title":"Glycosylated inositol phospholipid from <italic>Entamoeba histolytica<\/italic>: identification and structural characterization.","volume":"145","author":"RA Vishwakarma","year":"2006","journal-title":"Mol Biochem Parasitol."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1084\/jem.186.9.1557","article-title":"Protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of <italic>Entamoeba histolytica<\/italic>.","volume":"186","author":"A Marinets","year":"1997","journal-title":"J Exp Med."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/S1367-5931(00)00151-4","article-title":"Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis.","volume":"4","author":"T Kinoshita","year":"2000","journal-title":"Curr Opin Chem Biol."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/emboj\/20.1.250","article-title":"PIG-M transfers the first mannose to glycosylphosphatidylinositol on the lumenal side of the ER.","volume":"20","author":"Y Maeda","year":"2001","journal-title":"Embo J."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"8381","DOI":"10.1128\/IAI.73.12.8381-8392.2005","article-title":"Reduction of cell surface glycosylphosphatidylinositol conjugates in <italic>Entamoeba histolytica<\/italic> by antisense blocking of <italic>E. histolytica<\/italic> GlcNAc-phosphatidylinositol deacetylase expression: effect on cell proliferation, endocytosis, and adhesion to target cells.","volume":"73","author":"D Vats","year":"2005","journal-title":"Infect Immun."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/0035-9203(78)90144-X","article-title":"A new medium for the axenic cultivation of <italic>Entamoeba histolytica<\/italic> and other <italic>Entamoeba<\/italic>.","volume":"72","author":"LS Diamond","year":"1978","journal-title":"Trans R Soc Trop Med Hyg."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1128\/EC.5.5.871-875.2006","article-title":"Stress by heat shock induces massive down regulation of genes and allows differential allelic expression of the Gal\/GalNAc lectin in <italic>Entamoeba histolytica<\/italic>.","volume":"5","author":"C Weber","year":"2006","journal-title":"Eukaryot Cell."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0166-6851(96)02771-5","article-title":"Tetracycline-controlled gene expression in <italic>Entamoeba histolytica<\/italic>.","volume":"84","author":"L Hamann","year":"1997","journal-title":"Mol Biochem Parasitol."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1006\/jmbi.2000.4042","article-title":"T-Coffee: A novel method for fast and accurate multiple sequence alignment.","volume":"302","author":"C Notredame","year":"2000","journal-title":"J Mol Biol."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/protein\/12.1.3","article-title":"Machine learning approaches for the prediction of signal peptides and other protein sorting signals.","volume":"12","author":"H Nielsen","year":"1999","journal-title":"Protein Eng."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S0968-0004(98)01336-X","article-title":"PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization.","volume":"24","author":"K Nakai","year":"1999","journal-title":"Trends Biochem Sci."},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1110\/ps.0214502","article-title":"Transmembrane helix predictions revisited.","volume":"11","author":"CP Chen","year":"2002","journal-title":"Protein Sci."},{"key":"ref21","first-page":"175","article-title":"A hidden Markov model for predicting transmembrane helices in protein sequences.","volume":"6","author":"EL Sonnhammer","year":"1998","journal-title":"Proc Int Conf Intell Syst Mol Biol."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1006\/jmbi.1998.2107","article-title":"Principles governing amino acid composition of integral membrane proteins: application to topology prediction.","volume":"283","author":"GE Tusnady","year":"1998","journal-title":"J Mol Biol."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0076-6879(96)66033-9","article-title":"PHD: predicting one-dimensional protein structure by profile-based neural networks.","volume":"266","author":"B Rost","year":"1996","journal-title":"Methods Enzymol."},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1002\/pro.5560050824","article-title":"Topology prediction for helical transmembrane proteins at 86% accuracy.","volume":"5","author":"B Rost","year":"1996","journal-title":"Protein Sci."},{"key":"ref25","first-page":"685","article-title":"TopPred II: an improved software for membrane protein structure predictions.","volume":"10","author":"MG Claros","year":"1994","journal-title":"Comput Appl Biosci."},{"key":"ref26","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1006\/jmbi.2001.5008","article-title":"Energetics, stability, and prediction of transmembrane helices.","volume":"312","author":"S Jayasinghe","year":"2001","journal-title":"J Mol Biol."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"10974","DOI":"10.1016\/S0021-9258(18)99115-5","article-title":"Cell-free biosynthesis of lipophosphoglycan from <italic>Leishmania donovani<\/italic>. Characterization of microsomal galactosyltransferase and mannosyltransferase activities.","volume":"266","author":"MA Carver","year":"1991","journal-title":"J Biol Chem."},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3208","DOI":"10.1128\/IAI.70.6.3208-3215.2002","article-title":"Fate of <italic>Entamoeba histolytica<\/italic> during establishment of amoebic liver abscess analyzed by quantitative radioimaging and histology.","volume":"70","author":"MC Rigothier","year":"2002","journal-title":"Infect Immun."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"7945","DOI":"10.1073\/pnas.95.14.7945","article-title":"Activity of the yeast MNN1 alpha-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases.","volume":"95","author":"CA Wiggins","year":"1998","journal-title":"Proc Natl Acad Sci U S A."},{"key":"ref30","doi-asserted-by":"crossref","first-page":"19566","DOI":"10.1074\/jbc.273.31.19566","article-title":"A common motif of eukaryotic glycosyltransferases is essential for the enzyme activity of large clostridial cytotoxins.","volume":"273","author":"C Busch","year":"1998","journal-title":"J Biol Chem."},{"key":"ref31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1128\/MMBR.64.1.13-33.2000","article-title":"Membrane topology and insertion of membrane proteins: search for topogenic signals.","volume":"64","author":"M Van Geest","year":"2000","journal-title":"Microbiol Mol Biol Rev."},{"key":"ref32","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/S0092-8674(00)80930-7","article-title":"Protein translocation: tunnel vision.","volume":"92","author":"KE Matlack","year":"1998","journal-title":"Cell."},{"key":"ref33","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1146\/annurev.cellbio.12.1.27","article-title":"Signal-mediated sorting of membrane proteins between the endoplasmic reticulum and the golgi apparatus.","volume":"12","author":"RD Teasdale","year":"1996","journal-title":"Annu Rev Cell Dev Biol."},{"key":"ref34","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1002\/j.1460-2075.1990.tb07513.x","article-title":"Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.","volume":"9","author":"MR Jackson","year":"1990","journal-title":"Embo J."},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1172\/JCI114911","article-title":"Lysis of complement-sensitive Entamoeba histolytica by activated terminal complement components. Initiation of complement activation by an extracellular neutral cysteine proteinase.","volume":"86","author":"SL Reed","year":"1990","journal-title":"J Clin Invest."},{"key":"ref36","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/CMR.13.2.196-206.2000","article-title":"Cysteine proteinases and the pathogenesis of amebiasis.","volume":"13","author":"X Que","year":"2000","journal-title":"Clin Microbiol Rev."},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1172\/JCI115931","article-title":"Inhibition of the complement membrane attack complex by the galactose-specific adhesion of <italic>Entamoeba histolytica<\/italic>.","volume":"90","author":"LL Braga","year":"1992","journal-title":"J Clin Invest."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1084\/jem.167.3.887","article-title":"Complement binding by two developmental stages of <italic>Leishmania major<\/italic> promastigotes varying in expression of a surface lipophosphoglycan.","volume":"167","author":"SM Puentes","year":"1988","journal-title":"J Exp Med."},{"key":"ref39","doi-asserted-by":"crossref","first-page":"9536","DOI":"10.1073\/pnas.1530604100","article-title":"The role(s) of lipophosphoglycan (LPG) in the establishment of <italic>Leishmania major<\/italic> infections in mammalian hosts.","volume":"100","author":"GF Spath","year":"2003","journal-title":"Proc Natl Acad Sci U S A."}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0000165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,4]],"date-time":"2021-09-04T05:29:34Z","timestamp":1630733374000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0000165"}},"subtitle":[],"editor":[{"given":"Anuradha","family":"Lohia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2008,2,13]]},"references-count":39,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2008,2,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0000165","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2,13]]}}}