{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:24:19Z","timestamp":1789586659124,"version":"build-2803163510"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2010,2,16]],"date-time":"2010-02-16T00:00:00Z","timestamp":1266278400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0000602","type":"journal-article","created":{"date-parts":[[2010,2,15]],"date-time":"2010-02-15T21:01:00Z","timestamp":1266267660000},"page":"e602","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":28,"title":["Detection of Wuchereria bancrofti L3 Larvae in Mosquitoes: A Reverse Transcriptase PCR Assay Evaluating Infection and Infectivity"],"prefix":"10.1371","volume":"4","author":[{"given":"Sandra J.","family":"Laney","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reda M. R.","family":"Ramzy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanan H.","family":"Helmy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoda A.","family":"Farid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameen A.","family":"Ashour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary J.","family":"Weil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,2,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/S0169-4758(97)01151-4","article-title":"Global mapping of lymphatic filariasis.","volume":"13","author":"E Michael","year":"1997","journal-title":"Parasitol Today"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/j.1365-3156.2004.01287.x","article-title":"Editorial: Lymphatic filariasis endemicity\u2013an indicator of poverty?","volume":"9","author":"DN Durrheim","year":"2004","journal-title":"Trop Med Int Health"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e317","DOI":"10.1371\/journal.pntd.0000317","article-title":"The global programme to eliminate lymphatic filariasis: health impact after 8 years.","volume":"2","author":"EA Ottesen","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1046\/j.1365-3156.2000.00620.x","article-title":"The global programme to eliminate lymphatic filariasis.","volume":"5","author":"EA Ottesen","year":"2000","journal-title":"Trop Med Int Health"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"S15","DOI":"10.1179\/000349802125002374","article-title":"Lymphatic filariasis elimination: progress in global programme development.","volume":"96","author":"DH Molyneux","year":"2002","journal-title":"Ann Trop Med Parasitol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.pt.2006.12.001","article-title":"Diagnostic tools for filariasis elimination programs.","volume":"23","author":"GJ Weil","year":"2007","journal-title":"Trends Parasitol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e344","DOI":"10.1371\/journal.pntd.0000344","article-title":"The impact of repeated rounds of mass drug administration with diethylcarbamazine plus albendazole on bancroftian filariasis in Papua New Guinea.","volume":"2","author":"GJ Weil","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/S0140-6736(06)68426-2","article-title":"Effect of yearly mass drug administration with diethylcarbamazine and albendazole on bancroftian filariasis in Egypt: a comprehensive assessment.","volume":"367","author":"RM Ramzy","year":"2006","journal-title":"Lancet"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"S41","DOI":"10.1179\/000349802125002356","article-title":"Development and standardization of a rapid, PCR-based method for the detection of <italic>Wuchereria bancrofti<\/italic> in mosquitoes, for xenomonitoring the human prevalence of bancroftian filariasis.","volume":"96","author":"SA Williams","year":"2002","journal-title":"Ann Trop Med Parasitol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"826","DOI":"10.4269\/ajtmh.2006.74.826","article-title":"A real-time PCR-based assay for detection of <italic>Wuchereria bancrofti<\/italic> DNA in blood and mosquitoes.","volume":"74","author":"RU Rao","year":"2006","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1475-2883-5-2","article-title":"PCR and dissection as tools to monitor filarial infection of <italic>Aedes polynesiensis<\/italic> mosquitoes in French Polynesia.","volume":"5","author":"C Plichart","year":"2006","journal-title":"Filaria J"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2883-6-5","article-title":"Correction: PCR and dissection as tools to monitor filarial infections of <italic>Aedes polynesiensis<\/italic> mosquitoes in French Polynesia.","volume":"6","author":"C Plichart","year":"2007","journal-title":"Filaria J"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"591","DOI":"10.4269\/ajtmh.2007.77.591","article-title":"Molecular xenomonitoring of lymphatic filariasis.","volume":"77","author":"MJ Bockarie","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"593","DOI":"10.4269\/ajtmh.2007.77.593","article-title":"A critical appraisal of molecular xenomonitoring as a tool for assessing progress toward elimination of Lymphatic Filariasis.","volume":"77","author":"HA Farid","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1475-2883-2-11","article-title":"PCR and Mosquito dissection as tools to monitor filarial infection levels following mass treatment.","volume":"2","author":"DS Goodman","year":"2003","journal-title":"Filaria J"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"502","DOI":"10.4269\/ajtmh.2007.76.502","article-title":"Persistence of <italic>Brugia malayi<\/italic> DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis.","volume":"76","author":"P Fischer","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1242\/dev.121.8.2471","article-title":"The <italic>Caenorhabditis elegans<\/italic> heterochronic gene pathway controls stage-specific transcription of collagen genes.","volume":"121","author":"Z Liu","year":"1995","journal-title":"Development"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e251","DOI":"10.1371\/journal.pntd.0000251","article-title":"A Reverse Transcriptase-PCR Assay for Detecting Filarial Infective Larvae in Mosquitoes.","volume":"2","author":"SJ Laney","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: A new generation of protein database search programs.","volume":"25","author":"MT Altschul SF","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/S0020-7519(97)00208-7","article-title":"<italic>Caenorhabditis elegans<\/italic> as a model for parasitic nematodes.","volume":"28","author":"TR Burglin","year":"1998","journal-title":"Int J Parasitol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool.","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4269\/ajtmh.2005.73.108","article-title":"Effects of combined diethylcarbamazine and albendazole treatment of bancroftian filariasis on parasite uptake and development in <italic>Culex pipiens L<\/italic>.","volume":"73","author":"HA Farid","year":"2005","journal-title":"Am J Trop Med Hyg"},{"key":"ref23","first-page":"93","article-title":"Host-parasite relationships of <italic>Wuchereria bancrofti<\/italic> and mosquito hosts, <italic>Culex pipiens L<\/italic>. and <italic>Aedes caspius pallas<\/italic>.","volume":"26","author":"AM Gad","year":"1996","journal-title":"J Egypt Soc Parasitol"},{"key":"ref24","first-page":"305","article-title":"Uptake and development of <italic>Wuchereria bancrofti<\/italic> in <italic>Culex pipiens L<\/italic>. and <italic>Aedes caspius pallas<\/italic>.","volume":"26","author":"AM Gad","year":"1996","journal-title":"J Egypt Soc Parasitol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/0012-1606(91)90253-Y","article-title":"<italic>cut-1<\/italic> a <italic>Caenorhabditis elegans<\/italic> gene coding for a dauer-specific noncollagenous component of the cuticle.","volume":"146","author":"MLF Sebastiano","year":"1991","journal-title":"Dev Biol"},{"key":"ref26","first-page":"207","article-title":"RT-PCR assay for the detection of infective (L3) larvae of lymphatic filarial parasite, <italic>Wuchereria bancrofti<\/italic>, in vector mosquito <italic>Culex quinquefasciatus<\/italic>.","volume":"45","author":"V Vasuki","year":"2008","journal-title":"J Vector Borne Dis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1051\/parasite\/1994013255","article-title":"Molecular phylogenetic studies on <italic>Brugia<\/italic> filariae using Hha I repeat sequences.","volume":"1","author":"H Xie","year":"1994","journal-title":"Parasite"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0000602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,23]],"date-time":"2021-10-23T01:43:18Z","timestamp":1634953398000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0000602"}},"subtitle":[],"editor":[{"given":"Elodie","family":"Ghedin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,2,16]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2010,2,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0000602","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,16]]}}}