{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T01:00:20Z","timestamp":1785632420632,"version":"3.56.0"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2010,7,20]],"date-time":"2010-07-20T00:00:00Z","timestamp":1279584000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0000750","type":"journal-article","created":{"date-parts":[[2010,7,20]],"date-time":"2010-07-20T21:06:31Z","timestamp":1279659991000},"page":"e750","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Jacob2 Lectin of the Entamoeba histolytica Cyst Wall Binds Chitin and Is Polymorphic"],"prefix":"10.1371","volume":"4","author":[{"given":"Sudip K.","family":"Ghosh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina L.","family":"Van Dellen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anirban","family":"Chatterjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuli","family":"Dey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashidul","family":"Haque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillips W.","family":"Robbins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Samuelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,7,20]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1056\/NEJMra022710","article-title":"Amebiasis.","volume":"348","author":"R Haque","year":"2003","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0065-308X(07)65002-7","article-title":"Structure and content of the <italic>Entamoeba histolytica<\/italic> genome.","volume":"65","author":"CG Clark","year":"2007","journal-title":"Adv Parasitol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1550-7408.1993.tb04926.x","article-title":"A redescription of <italic>Entamoeba histolytica<\/italic> Schaudinn, 1903 (emended Walker, 1911) separating it from <italic>Entamoeba dispar<\/italic> Brumpt, 1925.","volume":"40","author":"LS Diamond","year":"1993","journal-title":"J Eukaryot Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S1369-5274(00)00229-0","article-title":"Encystation in parasitic protozoa.","volume":"4","author":"D Eichinger","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0166-6851(03)00073-2","article-title":"Gene discovery in the <italic>Entamoeba invadens<\/italic> genome.","volume":"129","author":"Z Wang","year":"2003","journal-title":"Mol Biochem Parasitol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"4217","DOI":"10.1128\/IAI.68.7.4217-4224.2000","article-title":"The most abundant glycoprotein of amebic cyst walls (Jacob) is a lectin with five Cys-rich, chitin-binding domains.","volume":"68","author":"M Frisardi","year":"2000","journal-title":"Infect Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/EC.5.5.836-848.2006","article-title":"Unique posttranslational modifications of chitin-binding lectins of <italic>Entamoeba invadens<\/italic> cyst walls.","volume":"5","author":"KL Van Dellen","year":"2006","journal-title":"Eukaryotic Cell"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0166-6851(96)02817-4","article-title":"Cloning and expression of chitinases of <italic>Entamoebae<\/italic>.","volume":"85","author":"H de la Vega","year":"1997","journal-title":"Mol Biochem Parasitol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.exppara.2009.07.008","article-title":"<italic>Entamoeba invadens<\/italic>: cloning and molecular characterization of chitinases.","volume":"123","author":"T Dey","year":"2009","journal-title":"Exp Parasitol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1128\/IAI.70.6.3259-3263.2002","article-title":"<italic>Entamoeba histolytica<\/italic> lectins contain unique 6-Cys or 8-Cys chitin-binding domains.","volume":"70","author":"K Van Dellen","year":"2002","journal-title":"Infect Immun"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e1000498","DOI":"10.1371\/journal.ppat.1000498","article-title":"Evidence for a \u201cwattle and daub\u201d model of the cyst wall of entamoeba.","volume":"5","author":"A Chatterjee","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/nature03291","article-title":"The genome of the protist parasite <italic>Entamoeba histolytica<\/italic>.","volume":"433","author":"B Loftus","year":"2005","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.molbiopara.2006.03.002","article-title":"The cyst wall of <italic>Entamoeba invadens<\/italic> contains chitosan (deacetylated chitin).","volume":"48","author":"S Das","year":"2006","journal-title":"Mol Biochem Parasitol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1128\/EC.5.1.203-206.2006","article-title":"Heterologous expression of an <italic>Entamoeba histolytica<\/italic> chitin synthase in <italic>Saccharomyces cerevisiae<\/italic>.","volume":"5","author":"KL Van Dellen","year":"2006","journal-title":"Eukaryotic Cell"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1111\/j.1462-5822.2006.00882.x","article-title":"Identification of developmentally regulated genes in <italic>Entamoeba histolytica<\/italic>: insights into mechanisms of stage conversion in a protozoan parasite.","volume":"9","author":"GM Ehrenkaufer","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"3815","DOI":"10.1128\/JCM.38.10.3815-3821.2000","article-title":"Molecular epidemiology of <italic>Entamoebae<\/italic>: Evidence of a bottleneck (demographic sweep) and transcontinental spread of diploid parasites.","volume":"38","author":"SK Ghosh","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4081","DOI":"10.1128\/JCM.40.11.4081-4090.2002","article-title":"Remarkable genetic polymorphism among <italic>Entamoeba histolytica<\/italic> isolates from a limited geographic area.","volume":"40","author":"A Haghighi","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0166-6851(93)90006-J","article-title":"A family of transcripts (K2) of <italic>Entamoeba histolytica<\/italic> contains polymorphic repetitive regions with highly conserved elements.","volume":"59","author":"S Kohler","year":"1993","journal-title":"Mol Biochem Parasitol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1006\/expr.1993.1105","article-title":"<italic>Entamoeba histolytica<\/italic>: a method for isolate identification.","volume":"77","author":"CG Clark","year":"1993","journal-title":"Exp Parasitol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"3248","DOI":"10.1073\/pnas.88.8.3248","article-title":"Sequence of a cysteine-rich galactose-specific lectin of <italic>Entamoeba histolytica<\/italic>.","volume":"88","author":"BJ Mann","year":"1991","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1128\/IAI.62.4.1166-1170.1994","article-title":"Protection of gerbils from amebic liver abscess by immunization with a recombinant <italic>Entamoeba histolytica<\/italic> antigen.","volume":"62","author":"T Zhang","year":"1994","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1086\/320740","article-title":"Amebiasis and mucosal IgA antibody against the <italic>Entamoeba histolytica<\/italic> adherence lectin in Bangladeshi children.","volume":"183","author":"R Haque","year":"2001","journal-title":"J Infect Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1128\/IAI.74.2.904-909.2006","article-title":"<italic>Entamoeba histolytica<\/italic> infection in children and protection from subsequent amebiasis.","volume":"74","author":"R Haque","year":"2006","journal-title":"Infect Immun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1586\/14760584.4.5.657","article-title":"Vaccine prospects for amebiasis.","volume":"4","author":"OA Chaudhry","year":"2005","journal-title":"Expert Rev Vaccines"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.","volume":"25","author":"SF Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","article-title":"A combined transmembrane topology and signal peptide prediction method.","volume":"338","author":"L K\u00e4ll","year":"2004","journal-title":"J Mol Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0166-6851(96)02733-8","article-title":"Overexpression of P-glycoprotein gene 1 by transfected <italic>Entamoeba histolytica<\/italic> confers emetine-resistance.","volume":"82","author":"SK Ghosh","year":"1996","journal-title":"Mol Biochem Parasitol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/PL00006478","article-title":"Evolution of chitin-binding proteins in invertebrates.","volume":"48","author":"Z Shen","year":"1999","journal-title":"J Mol Evol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"18355","DOI":"10.1074\/jbc.M800725200","article-title":"Unique Asn-linked oligosaccharides of the human pathogen <italic>Entamoeba histolytica.<\/italic>","volume":"283","author":"P Magnelli","year":"2008","journal-title":"J Biol Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1128\/JCM.44.2.632-634.2006","article-title":"Multilocus genotyping of <italic>Cryptosporidium sp.<\/italic> isolates from human immunodeficiency virus-infected individuals in South India.","volume":"44","author":"D Muthusamy","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0001-706X(93)90032-7","article-title":"Molecular variation in <italic>Plasmodium falciparum<\/italic>: polymorphic antigens of asexual erythrocytic stages.","volume":"53","author":"RF Anders","year":"1993","journal-title":"Acta Trop"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"13040","DOI":"10.1073\/pnas.94.24.13040","article-title":"<italic>Plasmodium falciparum<\/italic> antigenic diversity: evidence of clonal population structure.","volume":"94","author":"SM Rich","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"5842","DOI":"10.1128\/JCM.43.12.5842-5847.2005","article-title":"Use of PCR amplification of tRNA gene-linked short tandem repeats for genotyping <italic>Entamoeba histolytica<\/italic>.","volume":"43","author":"IK Ali","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref34","first-page":"274","article-title":"A proposal for a molecular biologic system for classifying isolates of <italic>Entamoeba histolytica<\/italic> and <italic>Entamoeba dispar<\/italic>.","volume":"28","author":"J Samuelson","year":"1997","journal-title":"Arch Med Res"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0000750","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T07:48:04Z","timestamp":1591516084000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0000750"}},"subtitle":[],"editor":[{"given":"Daniel","family":"Eichinger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,7,20]]},"references-count":34,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2010,7,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0000750","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,20]]}}}