{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:11:54Z","timestamp":1791850314092,"version":"4.3.4"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"5","content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0001147","type":"journal-article","created":{"date-parts":[[2011,5,3]],"date-time":"2011-05-03T20:16:00Z","timestamp":1304453760000},"page":"e1147","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["A New Approach to Dengue Fatal Cases Diagnosis: NS1 Antigen Capture in Tissues"],"prefix":"10.1371","volume":"5","author":[{"given":"Monique da Rocha Queiroz","family":"Lima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita Maria Ribeiro","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann Gon\u00e7alves","family":"Schatzmayr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria Bispo","family":"de Filippis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Limonta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia Barreto","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,5,3]]},"reference":[{"key":"ref1","article-title":"Fact sheet No 117, Dengue and dengue haemorrhagic fever.","year":"2009"},{"key":"ref2","first-page":"476","article-title":"Pathogenesis of dengue: challenges to molecular biology Science","volume":"239","author":"SB Halstead","year":"1988"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1590\/S0074-02761986000200019","article-title":"An outbreak of dengue virus at Rio de Janeiro.","volume":"81","author":"HG Schatzmayr","year":"1986","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"9599","key":"ref4","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1016\/S0140-6736(07)61687-0","article-title":"Dengue.","volume":"370","author":"SB Halstead","year":"2007","journal-title":"Lancet."},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1128\/JCM.38.3.1053-1057.2000","article-title":"An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients.","volume":"38","author":"PR Young","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"8","key":"ref6","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1086\/343813","article-title":"High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever.","volume":"186","author":"DH Libraty","year":"2002","journal-title":"J Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1128\/JCM.40.02.376-381.2002","article-title":"Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections.","volume":"40","author":"S Alcon","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1128\/CVI.00229-06","article-title":"Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum.","volume":"13","author":"P Dussart","year":"2006","journal-title":"Clin Vaccine Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1128\/JCM.00777-06","article-title":"Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections.","volume":"44","author":"H Xu","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jviromet.2006.11.001","article-title":"Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection.","volume":"140","author":"V Kumarasamy","year":"2007","journal-title":"J Virol Methods"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.diagmicrobio.2007.07.011","article-title":"Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos.","volume":"60","author":"SD Blacksell","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.diagmicrobio.2009.01.002","article-title":"Evaluation of dengue NS1 test kits for the diagnosis of dengue fever.","volume":"64","author":"WJ McBride","year":"2009","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jviromet.2008.10.016","article-title":"Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA.","volume":"155","author":"S Zainah","year":"2009","journal-title":"J Virol Methods"},{"issue":"7","key":"ref14","doi-asserted-by":"crossref","first-page":"e738","DOI":"10.1371\/journal.pntd.0000738","article-title":"Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil.","volume":"4","author":"MRQ Lima","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S1201-9712(99)90033-4","article-title":"Fatal dengue hemorrhagic fever in Cuba, 1997.","volume":"3","author":"MG Guzm\u00e1n","year":"1999","journal-title":"Int J Infect Dis"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"e77","DOI":"10.1016\/j.ijid.2008.06.028","article-title":"Fatal severe dengue and cell death in sickle cell disease during the 2001-2002 Havana dengue epidemic.","volume":"13","author":"D Limonta","year":"2009","journal-title":"Int J Infect Dis"},{"issue":"8","key":"ref17","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1086\/383043","article-title":"Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization.","volume":"189","author":"K Jessie","year":"2004","journal-title":"J Infect Dis"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"408","DOI":"10.4269\/ajtmh.1991.45.408","article-title":"Demonstration of yellow fever and dengue antigens in formalin-fixed paraffin-embedded human liver by immunohistochemical analysis.","volume":"45","author":"WC Hall","year":"1991","journal-title":"Am J Trop Med Hyg"},{"key":"ref19","first-page":"204","article-title":"Retrospective study on dengue fatal cases.","volume":"16","author":"MP Miagostovich","year":"1997","journal-title":"Clin Neuropathol"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.jcv.2007.04.024","article-title":"Apoptosis in tissues from fatal dengue shock syndrome.","volume":"40","author":"D Limonta","year":"2007","journal-title":"J Clin Virol"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"416","DOI":"10.4269\/ajtmh.2009.80.416","article-title":"Tropism of dengue virus in mice and humans defined by viral nonstructural protein 3-specific immunostaining.","volume":"80","author":"SJ Balsitis","year":"2009","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jviromet.2008.09.023","article-title":"A retrospective survey of dengue virus infection in fatal cases from an epidemic in Brazil.","volume":"155","author":"JM de Ara\u00fajo","year":"2009","journal-title":"J Virol Methods"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1099\/0022-1317-40-3-531","article-title":"Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses.","volume":"40","author":"A Igarashi","year":"1978","journal-title":"J Gen Virol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"158","DOI":"10.4269\/ajtmh.1984.33.158","article-title":"Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses.","volume":"33","author":"DJ Gubler","year":"1984","journal-title":"Am J Trop Med Hyg"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/JCM.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.","volume":"30","author":"RS Lanciotti","year":"1992","journal-title":"J Clin Microbiol"},{"issue":"1\u20132","key":"ref26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0166-0934(01)00280-4","article-title":"Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype-specific 3\u2032 noncoding sequences.","volume":"95","author":"HS Houng","year":"2001","journal-title":"J Virol Methods"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/CMR.11.3.480","article-title":"Dengue and dengue hemorrhagic fever.","volume":"11","author":"DJ Gubler","year":"1998","journal-title":"Clin Microbiol Rev"},{"issue":"9571","key":"ref28","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1016\/S0140-6736(07)60376-6","article-title":"Clinical, educational, and epidemiological value of autopsy.","volume":"369","author":"JL Burton","year":"2007","journal-title":"Lancet"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0928-0197(94)00032-P","article-title":"Diagnosing dengue virus infection in archived autopsy tissues by means of the in situ PCR method: a case report.","volume":"3","author":"D Kangwanpong","year":"1995","journal-title":"Clin Diagn Virol"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0166-0934(93)90121-7","article-title":"Detection of dengue virus by in situ hybridization.","volume":"41","author":"H Killen","year":"1993","journal-title":"J Virol Methods"},{"issue":"9","key":"ref31","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0046-8177(99)90230-7","article-title":"Report of a fatal case of dengue infection with hepatitis: demonstration of dengue antigens in hepatocytes and liver apoptosis.","volume":"30","author":"A Couvelard","year":"1999","journal-title":"Hum Pathol"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1006\/viro.2000.0524","article-title":"Involvement of oxidative stress, NF-IL-6, and RANTES expression in dengue-2-virus-infected human liver cells.","volume":"276","author":"YL Lin","year":"2000","journal-title":"Virology"},{"issue":"9209","key":"ref33","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/S0140-6736(00)02036-5","article-title":"Neurological manifestations of dengue infection.","volume":"355","author":"T Solomon","year":"2000","journal-title":"Lancet"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0167-5273(98)00008-4","article-title":"Cardiac involvement in Dengue Haemorrhagic Fever.","volume":"64","author":"JP Wali","year":"1998","journal-title":"Int J Cardiol"},{"issue":"7\u20138","key":"ref35","first-page":"477","article-title":"Disorders of atrio-ventricular conduction in patients with hemorrhagic dengue.","volume":"34","author":"E Donegani","year":"1986","journal-title":"Minerva Cardioangiol"},{"issue":"4","key":"ref36","first-page":"642","article-title":"Morbitz type I second degree AV block during recovery from dengue hemorrhagic fever.","volume":"31","author":"A Khongphatthallayothin","year":"2000","journal-title":"Southeast Asian J Trop Med Public Health"},{"issue":"6","key":"ref37","first-page":"569","article-title":"Transient ventricular arrhythmia as a cardiac manifestation in dengue haemorrhagic fever--a case report.","volume":"28","author":"SK Chuah","year":"1987"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1590\/S0074-02762007000200009","article-title":"Histopathological and ultrastructural aspects of mice lungs experimentally infected with dengue virus serotype 2.","volume":"102","author":"DF Barreto","year":"2007","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1590\/S1413-86702005000400012","article-title":"Pathologic study of a fatal case of dengue-3 virus infection in Rio de Janeiro, Brazil.","volume":"9","author":"CA Bas\u00edlio-de-Oliveira","year":"2005","journal-title":"Braz J Infect Dis"},{"issue":"2","key":"ref40","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s004280000329","article-title":"Liver histopathology and biological correlates in five cases of fatal dengue fever in Vietnamese children.","volume":"438","author":"MR Huerre","year":"2001","journal-title":"Virchows Arch"},{"issue":"6","key":"ref41","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1086\/507111","article-title":"Dengue fever-induced hemolytic uremic syndrome.","volume":"43","author":"WJ Wiersinga","year":"2006","journal-title":"Clin Infect Dis"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"121","DOI":"10.7589\/0090-3558-39.1.121","article-title":"European brown hare syndrome virus in free-ranging European brown hares from Argentina.","volume":"39","author":"K Fr\u00f6lich","year":"2003","journal-title":"J Wildl Dis"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1128\/JCM.40.6.2023-2030.2002","article-title":"Detection of West Nile virus antigen in mosquitoes and avian tissues by a monoclonal antibody-based capture enzyme immunoassay.","volume":"40","author":"AR Hunt","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1128\/JCM.30.4.947-950.1992","article-title":"Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates.","volume":"30","author":"TG Ksiazek","year":"1992","journal-title":"J Clin Microbiol"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0001147","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T15:40:14Z","timestamp":1686066014000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0001147"}},"subtitle":[],"editor":[{"given":"Alan L.","family":"Rothman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,5,3]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2011,5,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0001147","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,3]]}}}