{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:30:54Z","timestamp":1792737054666,"version":"4.5.0"},"reference-count":19,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2011,9,13]],"date-time":"2011-09-13T00:00:00Z","timestamp":1315872000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0001307","type":"journal-article","created":{"date-parts":[[2011,9,13]],"date-time":"2011-09-13T21:17:37Z","timestamp":1315948657000},"page":"e1307","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":79,"title":["Diagnostic Accuracy of a Loop-Mediated Isothermal PCR Assay for Detection of Orientia tsutsugamushi during Acute Scrub Typhus Infection"],"prefix":"10.1371","volume":"5","author":[{"given":"Daniel H.","family":"Paris","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Blacksell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pruksa","family":"Nawtaisong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemajittra","family":"Jenjaroen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achara","family":"Teeraratkul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirongrong","family":"Chierakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pacharee","family":"Kantipong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P. J.","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,9,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"256","DOI":"10.3201\/eid1202.050900","article-title":"Rickettsial infections and fever, Vientiane, Laos.","volume":"12","author":"S Phongmany","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.diagmicrobio.2006.01.014","article-title":"Determination and geographic distribution of <italic>Orientia tsutsugamushi<\/italic> serotypes in Thailand by nested polymerase chain reaction.","volume":"55","author":"J Manosroi","year":"2006","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1086\/510585","article-title":"Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion.","volume":"44","author":"SD Blacksell","year":"2007","journal-title":"Clin Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"900","DOI":"10.4269\/ajtmh.1976.25.900","article-title":"Adaptation of a microimmunofluorescence test to the study of human <italic>Rickettsia tsutsugamushi<\/italic> antibody.","volume":"25","author":"DM Robinson","year":"1976","journal-title":"Am J Trop Med Hyg"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"368","DOI":"10.4269\/ajtmh.2010.09-0233","article-title":"Diagnosis of Scrub Typhus.","volume":"82","author":"GC Koh","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.1469-0691.2008.02671.x","article-title":"A highly sensitive quantitative real-time PCR assay based on the <italic>groEL<\/italic> gene of contemporary Thai strains of <italic>Orientia tsutsugamushi<\/italic>.","volume":"15","author":"DH Paris","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers.","volume":"16","author":"K Nagamine","year":"2002","journal-title":"Mol Cell Probes"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA.","volume":"28","author":"T Notomi","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/bbrc.2001.5921","article-title":"Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation.","volume":"289","author":"Y Mori","year":"2001","journal-title":"Biochem Biophys Res Commun"},{"key":"ref10","first-page":"175","article-title":"Patient and sample-related factors that effect the success of in vitro isolation of <italic>Orientia tsutsugamushi<\/italic>.","volume":"38","author":"R Luksameetanasan","year":"2007","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"647","DOI":"10.4269\/ajtmh.1996.54.647","article-title":"Genotypic identification of <italic>Rickettsia tsutsugamushi<\/italic> by restriction fragment length polymorphism analysis of DNA amplified by the polymerase chain reaction.","volume":"54","author":"H Horinouchi","year":"1996","journal-title":"Am J Trop Med Hyg"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"351","DOI":"10.4269\/ajtmh.2004.70.351","article-title":"Development of a quantitative real-time polymerase chain reaction assay specific for <italic>Orientia tsutsugamushi<\/italic>.","volume":"70","author":"J Jiang","year":"2004","journal-title":"Am J Trop Med Hyg"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.trstmh.2007.11.001","article-title":"Real-time multiplex PCR assay for detection and differentiation of rickettsiae and orientiae.","volume":"102","author":"DH Paris","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.trstmh.2008.04.040","article-title":"Simple, rapid and sensitive detection of <italic>Orientia tsutsugamushi<\/italic> by loop-isothermal DNA amplification.","volume":"102","author":"DH Paris","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.4269\/ajtmh.1983.32.1101","article-title":"Diagnostic criteria for scrub typhus: probability values for immunofluorescent antibody and Proteus OXK agglutinin titers.","volume":"32","author":"GW Brown","year":"1983","journal-title":"Am J Trop Med Hyg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"497","DOI":"10.4269\/ajtmh.2002.67.497","article-title":"Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to <italic>Orientia tsutsugamushi<\/italic> in Thailand.","volume":"67","author":"RE Coleman","year":"2002","journal-title":"Am J Trop Med Hyg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"S203","DOI":"10.1086\/596576","article-title":"Scrub typhus: the geographic distribution of phenotypic and genotypic variants of <italic>Orientia tsutsugamushi<\/italic>.","volume":"48","author":"DJ Kelly","year":"2009","journal-title":"Clin Infect Dis"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.diagmicrobio.2007.07.011","article-title":"Evaluation of the PanBio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos.","volume":"60","author":"SD Blacksell","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e280","DOI":"10.1371\/journal.pntd.0000280","article-title":"Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum.","volume":"2","author":"P Dussart","year":"2008","journal-title":"PLoS Negl Trop Dis"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0001307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T15:53:03Z","timestamp":1589039583000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0001307"}},"subtitle":[],"editor":[{"given":"David H.","family":"Walker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,9,13]]},"references-count":19,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2011,9,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0001307","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,13]]}}}