{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:29:26Z","timestamp":1792373366830,"version":"4.4.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2011,9,13]],"date-time":"2011-09-13T00:00:00Z","timestamp":1315872000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0001310","type":"journal-article","created":{"date-parts":[[2011,9,13]],"date-time":"2011-09-13T21:17:49Z","timestamp":1315948669000},"page":"e1310","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":46,"title":["High-Throughput Analysis of Synthetic Peptides for the Immunodiagnosis of Canine Visceral Leishmaniasis"],"prefix":"10.1371","volume":"5","author":[{"given":"Ang\u00e9lica R.","family":"Faria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00edriam M.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio S.","family":"Giusta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Grimaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus L. O.","family":"Penido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo T.","family":"Gazzinelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9lida M.","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2011,9,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S0169-4758(00)01637-9","article-title":"The strange case of <italic>Leishmania infantum<\/italic>.","volume":"16","author":"IL Maur\u00edcio","year":"2000","journal-title":"Parasitol Today"},{"key":"ref2","first-page":"161","article-title":"Reservoirs of <italic>Leishmania donovani<\/italic> in Brazil.","volume":"7","author":"LM Deane","year":"1961","journal-title":"Rev Assoc Med Bras"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.vetpar.2006.03.008","article-title":"Seroepidemiological survey on canine leishmaniasis among dogs from an urban area of Brazil.","volume":"140","author":"F Dantas-Torres","year":"2006","journal-title":"Vet Parasitol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.vetpar.2007.03.004","article-title":"Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to <italic>Lutzomia longipalpis<\/italic> phlebotomine sand flies.","volume":"147","author":"EM Michalsky","year":"2007","journal-title":"Vet Parasitol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"287","DOI":"10.4269\/ajtmh.1995.52.287","article-title":"Control of zoonotic visceral leishmaniasis: is it time to change strategies?","volume":"52","author":"R Tesh","year":"1995","journal-title":"Am J Trop Med Hyg"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0304-4017(01)00643-4","article-title":"Evaluation of the <italic>Leishmania<\/italic> recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzyme-linked immunosorbent assay.","volume":"104","author":"A Scalone","year":"2002","journal-title":"Vet Parasitol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1128\/CDLI.4.5.598-603.1997","article-title":"Synthetic-peptide-based enzyme-linked immunosorbent assay for screening human serum or plasma for antibodies to human immunodeficiency virus type 1 and type 2.","volume":"4","author":"L Gonz\u00e1lez","year":"1997","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0304-4017(02)00327-8","article-title":"<italic>Taenia saginata<\/italic> derived synthetic peptides with potential for the diagnosis of bovine cysticercosis.","volume":"111","author":"E Ferrer","year":"2003","journal-title":"Vet Parasitol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1021\/pr101286y","article-title":"Analysis of <italic>Leishmania chagasi<\/italic> by 2-D Difference Gel Eletrophoresis (2-D DIGE) and Immunoproteomic: identification of novel candidate antigens for diagnostic tests and vaccine.","volume":"10","author":"MM Costa","year":"2011","journal-title":"J Proteome Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1017\/S0031182001007363","article-title":"Detection of <italic>Leishmania infantum<\/italic> by PCR, serology and cellular immune response in a cohort study of Brazilian dogs.","volume":"122","author":"RJ Quinnell","year":"2001","journal-title":"Parasitology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1745-7580-2-2","article-title":"Improved method for predicting linear B-cell epitopes.","volume":"2","author":"JE Larsen","year":"2006","journal-title":"Immunome Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B-cell epitopes in an antigen using recurrent neural network.","volume":"65","author":"S Saha","year":"2006","journal-title":"Proteins"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/jmr.893","article-title":"Predicting linear B-cell epitopes using string kernels.","volume":"21","author":"Y El-Manzalawy","year":"2008","journal-title":"J Mol Recog"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"9217","DOI":"10.1016\/S0040-4020(01)85612-X","article-title":"Spot-synthesis: an easy technique for the positionally addressable parallel chemical synthesis on a membrane support.","volume":"48","author":"R Frank","year":"1992","journal-title":"Tetrahedron"},{"key":"ref15","first-page":"149","article-title":"SPOT-synthesis: epitope analysis with arrays of synthetic peptides prepared on cellulose membranes.","volume":"66","author":"R Frank","year":"1996","journal-title":"Methods Mol Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1002\/jmr.825","article-title":"Systematic epitope analysis of the p26 EIAV core protein.","volume":"20","author":"A Soutullo","year":"2007","journal-title":"J Mol Recognit"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0014-5793(76)80276-1","article-title":"A polyamide support for solid-phase protein sequencing.","volume":"64","author":"E Atherton","year":"1976","journal-title":"FEBS Lett"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1590\/S0102-311X2004000100043","article-title":"Reflex\u00f5es sobre a qualidade do diagn\u00f3stico da leishmaniose visceral canina em inqu\u00e9ritos epidemiol\u00f3gicos: o caso da epidemia de Belo Horizonte, Minas Gerais, Brasil, 1993-1997.","volume":"20","author":"WA Alves","year":"2004","journal-title":"Cad Saude Publica,"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0167-5877(00)00115-X","article-title":"Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests.","volume":"45","author":"M Greiner","year":"2000","journal-title":"Prev Vet Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1037\/h0026256","article-title":"Weighted kappa: nominal scale agreement with provisions for scales disagreement of partial credit.","volume":"70","author":"J Cohen","year":"1968","journal-title":"Psychol Bull"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2307\/2529786","article-title":"An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers.","volume":"33","author":"JR Landis","year":"1977","journal-title":"Biometrics"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3","article-title":"Index for rating diagnostic tests.","volume":"3","author":"WJ Youden","year":"1950","journal-title":"Cancer"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1126\/science.3287615","article-title":"Measuring the accuracy of diagnostic systems.","volume":"240","author":"JA Swets","year":"1988","journal-title":"Science"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/rmv.602","article-title":"An introduction to epitope methods and software.","volume":"19","author":"X Yang","year":"2009","journal-title":"Rev Med Virol"},{"key":"ref25","first-page":"3","article-title":"Strength in numbers: Achieving greater accuracy in MHC-I binding prediction by combining the results from multiple prediction tools.","volume":"24","author":"B Trost","year":"2007","journal-title":"Immunome Res"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1128\/JCM.36.1.58-63.1998","article-title":"Multicomponent chimeric antigen for serodiagnosis of canine visceral leishmaniasis.","volume":"36","author":"M Soto","year":"1998","journal-title":"J Clin Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1128\/CVI.00420-06","article-title":"Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic <italic>Leishmania infantum<\/italic> visceral infections in dogs.","volume":"14","author":"R Porrozzi","year":"2007","journal-title":"Clin Vacc Immunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.vetpar.2008.07.028","article-title":"Methods for diagnosis of canine leishmaniasis and immune response to infection.","volume":"158","author":"C Maia","year":"2008","journal-title":"Vet Parasitol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.vetpar.2008.08.010","article-title":"Comparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogs.","volume":"157","author":"TC C\u00e2ndido","year":"2008","journal-title":"Vet Parasitol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.vetpar.2009.02.011","article-title":"A recombinant cysteine proteinase from <italic>Leishmania (Leishmania) chagasi<\/italic> as an antigen for delayed-type hypersensitivity assays and serodiagnosis of canine visceral leishmaniasis.","volume":"162","author":"PH Pinheiro","year":"2009","journal-title":"Vet Parasitol"},{"issue":"11","key":"ref31","doi-asserted-by":"crossref","first-page":"5515","DOI":"10.1128\/JCM.43.11.5515-5519.2005","article-title":"Evaluation of enzyme-Linked immunosorbent assays, an immunofluorescent-antibody test, and two rapid tests (immunochromatographic-dipstick and gel tests) for serological diagnosis of symptomatic and asymptomatic <italic>Leishmania<\/italic> infections in dogs.","volume":"43","author":"M Mettler","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref32","article-title":"Reprodutibilidade do diagn\u00f3stico sorol\u00f3gico da Leishmaniose Visceral Canina utilizando a rea\u00e7\u00e3o de ELISA em sangue dessecado em papel de filtro.","author":"GL Machado-Coelho","year":"2005"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.vetpar.2005.12.020","article-title":"Canine visceral leishmaniosis: A comparative analysis of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos and the IFI-leishmaniose-visceral-canina-Bio-Manguinhos kits.","volume":"137","author":"RA Lira","year":"2006","journal-title":"Vet Parasitol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1017\/S0031182001007363","article-title":"Detection of <italic>Leishmania infantum<\/italic> by PCR, serology and immune response in a cohort study of Brazilian dogs.","volume":"122","author":"RJ Quinnell","year":"2001","journal-title":"Parasitology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1128\/JCM.40.7.2352-2356.2002","article-title":"Rapid detection of <italic>Leishmania infantum<\/italic> infection in dogs: comparative study using an immunochromatographic dipstick test, enzyme-linked immunosorbent assay, and PCR.","volume":"40","author":"R Reithinger","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1017\/S0950268800051074","article-title":"Serological diagnosis of leishmaniasis: on detecting infection as well as disease.","volume":"103","author":"C Dye","year":"1993","journal-title":"Epidemiol and Infect"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1590\/S0074-02762005000200015","article-title":"Evaluation of enzyme-linked immunosorbent assay using crude <italic>Leishmania<\/italic> and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis.","volume":"100","author":"EY Ros\u00e1rio","year":"2005","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4269\/ajtmh.81.1.27","article-title":"Comparative study of serologic tests for the diagnosis of asymptomatic visceral leishmaniasis in an endemic area.","volume":"81","author":"HD Romero","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetpar.2007.07.007","article-title":"The role of dogs as reservoirs of <italic>Leishmania<\/italic> parasites, with emphasis on <italic>Leishmania (Leishmania) infantum<\/italic> and <italic>Leishmania (Viannia) braziliensis<\/italic>.","volume":"149","author":"F Dantas-Torres","year":"2007","journal-title":"Vet Parasitol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0035-9203(03)90146-5","article-title":"Wild and synanthropic hosts of <italic>Leishmania (Viannia) braziliensis<\/italic> in the endemic cutaneous leishmaniasis locality of Amaraji, Pernambuco State, Brazil.","volume":"97","author":"SP Brand\u00e3o-Filho","year":"2003","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1128\/IAI.68.4.1760-1764.2000","article-title":"Comparison of the immune profile of nonhealing cutaneous leishmaniasis patients with those with active lesions and those who have recovered from infection.","volume":"68","author":"S Ajdary","year":"2000","journal-title":"Infect and Immun"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.exppara.2007.03.003","article-title":"<italic>Leishmania (Viannia) braziliensis:<\/italic> epidemiology of canine cutaneous leishmaniasis in the state of Paran\u00e1 (Brazil).","volume":"117","author":"EA Castro","year":"2007","journal-title":"Exp Parasitol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1590\/S0102-311X2004000500012","article-title":"Triatomine infestation and vector-borne transmission of Chagas disease in northwest and central Paran\u00e1, Brazil.","volume":"20","author":"AL Falavigna-Guilherme","year":"2004","journal-title":"Cad Saude Publica"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1128\/CDLI.12.5.647-653.2005","article-title":"Development of recombinant chimeric antigen expressing immunodominant B epitopes of <italic>Leishmania infantum<\/italic> for serodiagnosis of visceral leishmaniasis.","volume":"12","author":"A Boarino","year":"2005","journal-title":"Clin Diag Lab Immun"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.parint.2010.11.002","article-title":"Development of an immunochromatographic strip for the rapid detection of <italic>Toxoplasma gondii<\/italic> circulating antigens.","volume":"60","author":"Y Wang","year":"2011","journal-title":"Parasitol Int"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jviromet.2010.04.015","article-title":"Development of a convenient immunochromatographic strip for the diagnosis of infection with Japanese encephalitis virus in swine.","volume":"168","author":"Y Li","year":"2010","journal-title":"J Virol Meth"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1292\/jvms.09-0528","article-title":"A simple and rapid strip test for detection of antibodies to avian infectious bronchitis virus.","volume":"72","author":"J Zhang","year":"2010","journal-title":"J Vet Med Sci"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0001310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,4]],"date-time":"2021-12-04T16:11:03Z","timestamp":1638634263000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0001310"}},"subtitle":[],"editor":[{"given":"Ana","family":"Rodriguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,9,13]]},"references-count":47,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2011,9,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0001310","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,13]]}}}