{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:13:55Z","timestamp":1791191635460,"version":"4.1.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T00:00:00Z","timestamp":1354147200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0001927","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T22:40:50Z","timestamp":1354228850000},"page":"e1927","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":48,"title":["Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis"],"prefix":"10.1371","volume":"6","author":[{"given":"Estefania","family":"Calvo-\u00c1lvarez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nestor Adrian","family":"Guerrero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"\u00c1lvarez-Velilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher Fern\u00e1ndez","family":"Prada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Mar\u00eda","family":"Requena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Punz\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel \u00c1ngel","family":"Llamas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"Ar\u00e9valo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Rivas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Fresno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"P\u00e9rez-Pertejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Bala\u00f1a-Fouce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa M.","family":"Reguera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,11,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.cimid.2004.03.004","article-title":"Leishmaniasis: current situation and new perspectives","volume":"27","author":"P Desjeux","year":"2004","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1111\/j.1462-5822.2007.00995.x","article-title":"Applications of bioluminescence imaging to the study of infectious diseases","volume":"9","author":"M Hutchens","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.pt.2009.07.006","article-title":"Imaging <italic>Leishmania<\/italic> development in their host cells","volume":"25","author":"T Lang","year":"2009","journal-title":"Trends Parasitol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/IAI.73.2.695-702.2005","article-title":"Bioluminescence imaging of <italic>Toxoplasma gondii<\/italic> infection in living mice reveals dramatic differences between strains","volume":"73","author":"JP Saeij","year":"2005","journal-title":"Infect Immun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/nprot.2006.69","article-title":"Real-time <italic>in vivo<\/italic> imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice","volume":"1","author":"B Franke-Fayard","year":"2006","journal-title":"Nat Protoc"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"e7881","DOI":"10.1371\/journal.pone.0007881","article-title":"Visualisation and quantitative analysis of the rodent malaria liver stage by real time imaging","volume":"4","author":"IH Ploemen","year":"2009","journal-title":"PLoS One"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1462-5822.2004.00468.x","article-title":"Bioluminescent <italic>Leishmania<\/italic> expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice","volume":"7","author":"T Lang","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"600","DOI":"10.4269\/ajtmh.2005.72.600","article-title":"Colorimetric assay for screening compounds against <italic>Leishmania<\/italic> amastigotes grown in macrophages","volume":"72","author":"FS Buckner","year":"2005","journal-title":"Am J Trop Med Hyg"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1128\/AAC.45.4.1168-1173.2001","article-title":"DNA transformation of <italic>Leishmania infantum<\/italic> axenic amastigotes and their use in drug screening","volume":"45","author":"D Sereno","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e34","DOI":"10.1371\/journal.pntd.0000034","article-title":"Optimization of topical therapy for <italic>Leishmania major<\/italic> localized cutaneous leishmaniasis using a reliable C57BL\/6 model","volume":"1","author":"H Lecoeur","year":"2007","journal-title":"PLoS Negl Trop Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e486","DOI":"10.1371\/journal.pntd.0000486","article-title":"Bioluminescent imaging of <italic>Trypanosoma brucei<\/italic> shows preferential testis dissemination, which may hamper drug efficacy in sleeping sickness","volume":"3","author":"F Claes","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nmeth819","article-title":"A guide to choosing fluorescent proteins","volume":"2","author":"NC Shaner","year":"2005","journal-title":"Nat Methods"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0166-6851(96)02580-7","article-title":"Use of the green fluorescent protein as a marker in transfected <italic>Leishmania<\/italic>","volume":"77","author":"DS Ha","year":"1996","journal-title":"Mol Biochem Parasitol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"3654","DOI":"10.1128\/AAC.45.12.3654-3656.2001","article-title":"Expression of green fluorescent protein as a marker for effects of antileishmanial compounds <italic>in vitro<\/italic>","volume":"45","author":"SW Kamau","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"400","DOI":"10.4269\/ajtmh.2004.71.400","article-title":"Short report: fluorescent <italic>Leishmania<\/italic>: application to anti-leishmanial drug testing","volume":"71","author":"N Singh","year":"2004","journal-title":"Am J Trop Med Hyg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1007\/s00436-002-0706-4","article-title":"A microplate assay for <italic>Leishmania amazonensis<\/italic> promastigotes expressing multimeric green fluorescent protein","volume":"89","author":"MM Chan","year":"2003","journal-title":"Parasitol Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1538\/expanim.52.109","article-title":"Applications of recombinant <italic>Leishmania amazonensis<\/italic> expressing <italic>egfp<\/italic> or the <italic>\u03b2-galactosidase<\/italic> gene for drug screening and histopathological analysis","volume":"52","author":"T Okuno","year":"2003","journal-title":"Exp Anim"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1007\/s00436-005-1339-1","article-title":"Refractoriness to the treatment of sodium stibogluconate in Indian kala-azar field isolates persist in <italic>in vitro<\/italic> and <italic>in vivo<\/italic> experimental models","volume":"96","author":"A Dube","year":"2005","journal-title":"Parasitol Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jim.2008.01.004","article-title":"Intracellular parasite kill: flow cytometry and NO detection for rapid discrimination between anti-leishmanial activity and macrophage activation","volume":"333","author":"D Kram","year":"2008","journal-title":"J Immunol Methods"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1093\/jac\/dkp206","article-title":"Transgenic <italic>Leishmania donovani<\/italic> clinical isolates expressing green fluorescent protein constitutively for rapid and reliable <italic>ex vivo<\/italic> drug screening","volume":"64","author":"N Singh","year":"2009","journal-title":"J Antimicrob Chemother"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.exppara.2010.12.006","article-title":"Fluorescent <italic>Leishmania<\/italic> species: development of stable GFP expression and its application for <italic>in vitro<\/italic> and <italic>in vivo<\/italic> studies","volume":"127","author":"A Bolhassani","year":"2011","journal-title":"Exp Parasitol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.actatropica.2011.11.015","article-title":"Improvement of the green fluorescent protein reporter system in <italic>Leishmania<\/italic> spp. for the in vitro and in vivo screening of antileishmanial drugs","volume":"122","author":"SA Pulido","year":"2012","journal-title":"Acta Trop"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0166-6851(00)00195-X","article-title":"Targeted integration into a rRNA locus results in uniform and high level expression of transgenes in <italic>Leishmania<\/italic> amastigotes","volume":"107","author":"A Mi\u03b2litz","year":"2000","journal-title":"Mol Biochem Parasitol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1128\/CVI.00270-08","article-title":"Real-time <italic>in vivo<\/italic> green fluorescent protein imaging of a murine leishmaniasis model as a new tool for <italic>Leishmania<\/italic> vaccine and drug discovery","volume":"15","author":"SR Mehta","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.exppara.2010.05.014","article-title":"Imaging of the host\/parasite interplay in cutaneous leishmaniasis","volume":"126","author":"OR Millington","year":"2010","journal-title":"Exp Parasitol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1126\/science.1159194","article-title":"<italic>In vivo<\/italic> imaging reveals an essential role for neutrophils in leishmaniasis transmitted by sandflies","volume":"321","author":"N Peters","year":"2008","journal-title":"Science"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e1002536","DOI":"10.1371\/journal.ppat.1002536","article-title":"Efficient capture of infected neutrophils by dendritic cells in the skin inhibits the early anti-<italic>Leishmania<\/italic> response","volume":"8","author":"F Ribeiro-Gomes","year":"2012","journal-title":"PLoS Pathog"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1128\/IAI.00338-10","article-title":"Leukocytes infiltrate the skin and draining lymph nodes in response to the protozoan <italic>Leishmania infantum<\/italic> chagasi","volume":"79","author":"CJ Thalhofer","year":"2011","journal-title":"Infect Immun"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.micinf.2009.09.014","article-title":"Sorting of <italic>Leishmania<\/italic>-bearing dendritic cells reveals subtle parasite-induced modulation of host-cell gene expression","volume":"12","author":"H Lecoeur","year":"2010","journal-title":"Microbes Infect"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e1000494","DOI":"10.1371\/journal.ppat.1000494","article-title":"iNOS-Producing inflammatory dendritic cells constitute the major infected cell type during the chronic <italic>Leishmania major<\/italic> infection phase of C57BL\/6 resistant mice","volume":"5","author":"C De Trez","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e19851","DOI":"10.1371\/journal.pone.0019851","article-title":"Visualisation of <italic>Leishmania donovani<\/italic> fluorescent hybrids during early stage development in the sandfly vector","volume":"6","author":"J Sadlova","year":"2011","journal-title":"PLoS One"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"10125","DOI":"10.1073\/pnas.0802331105","article-title":"Quantification of the infectious dose of <italic>Leishmania major<\/italic> transmitted to the skin by single sandflies","volume":"105","author":"N Kimblin","year":"2008","journal-title":"Proc Nat Acad Sci USA"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1002\/biot.200900035","article-title":"Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and <italic>in vitro<\/italic> live imaging of <italic>Plasmodium<\/italic> parasites","volume":"4","author":"S Graewe","year":"2009","journal-title":"Biotechnol J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1002\/jbio.200710024","article-title":"Fluorescent proteins for single-molecule fluorescence applications","volume":"1","author":"B Seefeldt","year":"2008","journal-title":"J Biophotonics"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1186\/1756-3305-4-150","article-title":"<italic>Leishmania infantum<\/italic> HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation","volume":"4","author":"J Carrion","year":"2011","journal-title":"Parasit Vectors"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"35172","DOI":"10.1074\/jbc.M505559200","article-title":"The translational efficiencies of the two <italic>Leishmania infantum<\/italic> HSP70 mRNAs, differing in their 3\u2032-untranslated regions, are affected by shifts in the temperature of growth through different mechanisms","volume":"280","author":"C Folgueira","year":"2005","journal-title":"J Biol Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1038\/nbt1037","article-title":"Improved monomeric red, orange and yellow fluorescent proteins derived from <italic>Discosoma<\/italic> sp. red fluorescent protein","volume":"22","author":"NC Shaner","year":"2004","journal-title":"Nat Biotechnol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1128\/MCB.10.3.1084","article-title":"Stable transfection of the human parasite <italic>Leishmania major<\/italic> delineates a 30-kilobase region sufficient for extrachromosomal replication and expression","volume":"10","author":"GM Kapler","year":"1990","journal-title":"Mol Cell Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1006\/expr.2001.4656","article-title":"A lipophosphoglycan-independent method for isolation of infective <italic>Leishmania<\/italic> metacyclic promastigotes by density gradient centrifugation","volume":"99","author":"GF Sp\u00e4th","year":"2001","journal-title":"Exp Parasitol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1006\/expr.1996.4139","article-title":"<italic>Leishmania major<\/italic>: promastigotes induce expression of a subset of chemokine genes in murine macrophages","volume":"85","author":"EL Racoosin","year":"1997","journal-title":"Exp Parasitol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.exppara.2009.03.014","article-title":"<italic>Leishmania donovani<\/italic> lacking the Golgi GDP-Man transporter LPG2 exhibit attenuated virulence in mammalian hosts","volume":"122","author":"U Gaur","year":"2009","journal-title":"Exp Parasitol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1126\/science.6701528","article-title":"Identification of an infective stage of <italic>Leishmania<\/italic> promastigotes","volume":"223","author":"DL Sacks","year":"1984","journal-title":"Science"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"e962","DOI":"10.1371\/journal.pntd.0000962","article-title":"Identification of small molecule lead compounds for visceral leishmaniasis using a novel <italic>ex vivo<\/italic> splenic explant model system","volume":"5","author":"Y Osorio","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0169-4758(96)40010-2","article-title":"A simple method for quantifying <italic>Leishmania<\/italic> in tissues of infected animals","volume":"13","author":"HC Lima","year":"1997","journal-title":"Parasitol Today"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.pt.2009.06.006","article-title":"Reporter genes facilitating discovery of drugs targeting protozoan parasites","volume":"25","author":"A Dube","year":"2009","journal-title":"Trends Parasitol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.chembiol.2009.10.009","article-title":"Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals","volume":"16","author":"MZ Lin","year":"2009","journal-title":"Chem Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0166-6851(00)00270-X","article-title":"Episomal and stable expression of the luciferase reporter gene for quantifying <italic>Leishmania<\/italic> spp. infections in macrophages and in animal models","volume":"110","author":"G Roy","year":"2000","journal-title":"Mol Biochem Parasitol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1093\/jac\/dkp206","article-title":"Transgenic <italic>Leishmania donovani<\/italic> isolates expressing green fluorescent protein constitutively for rapid and reliable ex vivo drug screening","volume":"64","author":"N Singh","year":"2009","journal-title":"J Antimicrob Chemother"},{"key":"ref49","article-title":"The transcriptome of Leishmania major by deep RNA sequencing: transcript annotation and relative expression levels in the axenic promastigote stage","author":"A Rastrojo","year":"2012","journal-title":"Nucl Acids Res"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.molbiopara.2005.12.009","article-title":"Expression profiling by whole-genome interspecies microarray hybridization reveals differential gene expression in procyclic promastigotes, lesion-derived amastigotes, and axenic amastigotes in <italic>Leishmania mexicana<\/italic>","volume":"146","author":"TR Holzer","year":"2006","journal-title":"Mol Biochem Parasitol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1128\/AAC.41.4.818","article-title":"An axenic amastigote system for drug screening","volume":"41","author":"HL Callahan","year":"1997","journal-title":"Antimicrob Agents Chemother"},{"key":"ref52","doi-asserted-by":"crossref","DOI":"10.1007\/s00284-012-0209-1","article-title":"Development of luciferase expressing <italic>Leishmania donovani<\/italic> axenic amastigotes as primary model for in vitro screening of antileishmanial compounds","author":"Ravinder","year":"2012","journal-title":"Curr Microbiol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.molbiopara.2008.12.012","article-title":"Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of <italic>Leishmania infantum<\/italic>","volume":"165","author":"A Rochette","year":"2009","journal-title":"Mol Biochem Parasitol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.parint.2006.09.001","article-title":"Advances and perspectives in <italic>Leishmania<\/italic> cell based drug-screening procedures","volume":"56","author":"D Sereno","year":"2007","journal-title":"Parasitol Int"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mimet.2012.01.013","article-title":"A fluorometric method for evaluation of pharmacological activity against intracellular <italic>Leishmania<\/italic> amastigotes","volume":"89","author":"P Bilbao-Ramos","year":"2012","journal-title":"J Microbiol Methods"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/j.micinf.2011.05.014","article-title":"The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of <italic>Leishmania<\/italic>","volume":"13","author":"CH Hsiao","year":"2011","journal-title":"Microbes Infect"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1006\/expr.2001.4598","article-title":"Application of flow cytometry and microscopical methods to characterize the effect of herbal drugs on <italic>Leishmania<\/italic> spp","volume":"97","author":"A Plock","year":"2001","journal-title":"Exp Parasitol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"3776","DOI":"10.1128\/AAC.49.9.3776-3783.2005","article-title":"Use of <italic>Leishmania donovani<\/italic> field isolates expressing the luciferase reporter gene in in vitro drug screening","volume":"49","author":"S Ashutosh, Gupta","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"e1671","DOI":"10.1371\/journal.pntd.0001671","article-title":"An image-based high-content screening assay for compounds targeting intracellular <italic>Leishmania donovani<\/italic> amastigotes in human macrophages","volume":"6","author":"JL Siqueira-Neto","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"e1253","DOI":"10.1371\/journal.pntd.0001253","article-title":"A screen against <italic>Leishmania<\/italic> intracellular amastigotes: comparison to a promastigote screen and identification of a host cell-specific hit","volume":"5","author":"G De Muylder","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"5264","DOI":"10.1128\/AAC.00499-12","article-title":"Indotecan (LMP400) and AM13-55: Two novel indenoisoquinolines show potential for treating visceral leishmaniasis","volume":"56","author":"R Bala\u00f1a-Fouce","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"ref62","first-page":"pii 1980","article-title":"<italic>In vivo<\/italic> imaging of transgenic <italic>Leishmania<\/italic> parasites in a live host","volume":"41","author":"CJ Thalhofer","year":"2010","journal-title":"J Vis Exp"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1128\/AAC.01310-09","article-title":"Early curative applications of the aminoglycoside WR279396 on an experimental <italic>Leishmania major<\/italic>-loaded cutaneous site do not impair the acquisition of immunity","volume":"54","author":"H Lecoeur","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1002\/jbio.201000008","article-title":"Monitoring the efficacy of antimicrobial photodynamic therapy in a murine model of cutaneous leishmaniasis using <italic>L. major<\/italic> expressing GFP","volume":"3","author":"E Latorre-Esteves","year":"2010","journal-title":"J Biophotonics"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0001927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T05:52:28Z","timestamp":1594965148000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0001927"}},"subtitle":[],"editor":[{"given":"Genevieve","family":"Milon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,11,29]]},"references-count":64,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2012,11,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0001927","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,29]]}}}