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Geneva, Switzerland: World Health Organization."},{"key":"ref2","first-page":"43","article-title":"Buruli ulcer: first programme review meeting for West Africa\u2013summary report","volume":"84","journal-title":"Wkly Epidemiol Rec"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1440-0960.1985.tb01819.x","article-title":"Clinical features of <italic>Mycobacterium ulcerans<\/italic> infection","volume":"26","author":"J Hayman","year":"1985","journal-title":"Australas J Dermatol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"651","DOI":"10.3201\/eid0906.020485","article-title":"Histopathologic features of <italic>Mycobacterium ulcerans<\/italic> infection","volume":"9","author":"J Guarner","year":"2003","journal-title":"Emerging Infect Dis"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1128\/AAC.49.8.3182-3186.2005","article-title":"Efficacy of the Combination Rifampin-Streptomycin in Preventing Growth of <italic>Mycobacterium ulcerans<\/italic> in Early Lesions of Buruli Ulcer in Humans","volume":"49","author":"S Etuaful","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/S0140-6736(09)61962-0","article-title":"Antimicrobial treatment for early, limited <italic>Mycobacterium ulcerans<\/italic> infection: a randomised controlled trial","volume":"375","author":"WA Nienhuis","year":"2010","journal-title":"The Lancet"},{"key":"ref7","unstructured":"World Health Organisation (2006) Buruli ulcer: Prevention of disability (POD). Geneva: World Health Organisation WHO\/CDS\/NTD\/\/GBUI\/2006.12. 120p <comment>Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/buruli\/information\/publications\/BU-0POD-presentation.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/buruli\/information\/publications\/BU-0POD-presentation.pdf<\/ext-link> Assessed 5 October 2010<\/comment>."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1126\/science.283.5403.854","article-title":"Mycolactone: a polyketide toxin from <italic>Mycobacterium ulcerans<\/italic> required for virulence","volume":"283","author":"KM George","year":"1999","journal-title":"Science"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1084\/jem.20070234","article-title":"Selective suppression of dendritic cell functions by <italic>Mycobacterium ulcerans<\/italic> toxin mycolactone","volume":"204","author":"E Coutanceau","year":"2007","journal-title":"J Exp Med"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3928","DOI":"10.4049\/jimmunol.163.7.3928","article-title":"The inhibitory action of <italic>Mycobacterium ulcerans<\/italic> soluble factor on monocyte\/T cell cytokine production and NF-kappa B function","volume":"163","author":"AA Pahlevan","year":"1999","journal-title":"J Immunol"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"367","DOI":"10.3201\/eid0503.990307","article-title":"Emergence of a unique group of necrotizing mycobacterial diseases","volume":"5","author":"KM Dobos","year":"1999","journal-title":"Emerging Infect Dis"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Percival S, Cutting K (2010) Microbiology of Wounds. Boca Raton FL. Taylor and Francis Group: CRC Press 385p","DOI":"10.1201\/9781420079944"},{"key":"ref13","unstructured":"Baranoski S, Ayello EA (2008) Wound Care Essentials Practice Principles. Philadelphia: Lippincott Williams and Wilkins. 479p"},{"key":"ref14","unstructured":"Todar K (2008) The Normal Bacterial Flora of Humans. Todars online Textbook of Bacteriology. <comment>Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.textbookofbacteriology.net\" xlink:type=\"simple\">http:\/\/www.textbookofbacteriology.net<\/ext-link> Accessed 10<sup>th<\/sup> October 2010<\/comment>"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1097\/00044067-200208000-00005","article-title":"Evaluating and managing open skin wounds: colonization versus infection","volume":"13","author":"AB Wysocki","year":"2002","journal-title":"AACN Clin Issues"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1128\/CMR.14.2.244-269.2001","article-title":"Wound Microbiology and Associated Approaches to Wound Management","volume":"14","author":"PG Bowler","year":"2001","journal-title":"Clinical Microbiology Reviews"},{"issue":"2","key":"ref17","first-page":"123","article-title":"The significance of beta-haemolytic streptococci in chronic leg ulcers","volume":"72","author":"IG Schraibman","year":"1990","journal-title":"Ann R Coll Surg Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/modpathol.2010.219","article-title":"Interface membrane is the best sample for histopathological study to diagnose prosthetic joint infection","volume":"24","author":"G Bori","year":"2011","journal-title":"Modern Pathology"},{"key":"ref19","unstructured":"Collier M (2004) Recognition and Management of Wound Infections. <comment>Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.worldwidewounds.com\/2004\/january\/Collier\/Management-of-Wound-infections.html\" xlink:type=\"simple\">http:\/\/www.worldwidewounds.com\/2004\/january\/Collier\/Management-of-Wound-infections.html<\/ext-link>. Accessed: 10<sup>th<\/sup> October 2010<\/comment>"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1365-3156.2007.01990.x","article-title":"Diagnosis of <italic>Mycobacterium ulcerans<\/italic> infection (Buruli ulcer) at a treatment centre in Ghana: a retrospective analysis of laboratory results of clinically diagnosed cases","volume":"13","author":"E Mensah-Quainoo","year":"2008","journal-title":"Trop Med Int Health"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"900","DOI":"10.4269\/ajtmh.2011.11-0362","article-title":"Combining PCR with Microscopy to Reduce Costs of Laboratory Diagnosis of Buruli Ulcer","volume":"85","author":"D Yeboah-Manu","year":"2011","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1097\/00005373-197602000-00002","article-title":"The quantitative swab culture and smears: a quick, simple method for determining the number of viable aerobic bacteria on open wounds","volume":"16","author":"NS Levine","year":"1976","journal-title":"J Trauma"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1097\/00004045-199903000-00008","article-title":"Identification and evaluating wound infection","volume":"17","author":"NA Stotts","year":"1999","journal-title":"Home Health Nurse"},{"key":"ref24","first-page":"185","article-title":"\u201c\u00dcber die isolierte F\u00e4rbung der Schizomyceten in Schnitt- und Trockenpr\u00e4paraten\u201d (in German)","volume":"2","author":"HC Gram","year":"1884"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1021\/ac00171a028","article-title":"Laser desorption ionization of proteins with molecular masses exceeding 10,000 Daltons","volume":"60","author":"M Karas","year":"1988","journal-title":"Anal Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disk method","volume":"36","author":"AW Bauer","year":"1966","journal-title":"Am J Clin Pathol"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1128\/IAI.68.2.877-883.2000","article-title":"A <italic>Mycobacterium ulcerans<\/italic> toxin, mycolactone, causes apoptosis in guinea pig ulcers and tissue culture cells","volume":"68","author":"KM George","year":"2000","journal-title":"Infect Immun"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1111\/j.1462-5822.2005.00557.x","article-title":"<italic>Mycobacterium ulcerans<\/italic> toxic macrolide, mycolactone modulates the host immune response and cellular location of M. ulcerans in vitro and in vivo","volume":"7","author":"S Adusumilli","year":"2005","journal-title":"Cell Microbiol"},{"issue":"8","key":"ref29","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1111\/j.1462-5822.2005.00546.x","article-title":"Modulation of the host immune response by a transient intracellular stage of <italic>Mycobacterium ulcerans<\/italic>: the contribution of endogenous mycolactone toxin","volume":"7","author":"E Coutanceau","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1146\/annurev.mi.47.100193.004303","article-title":"Polyketide synthesis: prospects for hybrid antibiotics","volume":"47","author":"L Katz","year":"1993","journal-title":"Annu Rev Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1136\/jcp.34.7.701","article-title":"Investigation into the microbial flora of healing and non-healing decubitus ulcers","volume":"34","author":"RC Daltery","year":"1981","journal-title":"J Clin Pathol"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1097\/00000658-197302000-00001","article-title":"<italic>Providencia Stuartii<\/italic> sepsis: A new challenge in the treatment of thermal injury","volume":"177","author":"W Curreri","year":"1973","journal-title":"Annals of surgery"},{"key":"ref33","first-page":"1","article-title":"Multiple bacterial species reside in chronic wound: a longitudinal study","volume":"1","author":"K Gj\u00d8dsb\u00d8l","year":"2006","journal-title":"Int Wound J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"452","DOI":"10.12968\/jowc.1998.7.9.452","article-title":"Defining infection in chronic wounds: methodology. 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