{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:46:33Z","timestamp":1791236793990,"version":"4.1.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T00:00:00Z","timestamp":1369872000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0002239","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T17:04:49Z","timestamp":1369933489000},"page":"e2239","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":100,"title":["Cooler Temperatures Destabilize RNA Interference and Increase Susceptibility of Disease Vector Mosquitoes to Viral Infection"],"prefix":"10.1371","volume":"7","author":[{"given":"Zach N.","family":"Adelman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle A. E.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Wiley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta G.","family":"Murreddu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glady Hazitha","family":"Samuel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine M.","family":"Morazzani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Myles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,5,30]]},"reference":[{"key":"ref1","unstructured":"Confalonieri U, Menne R, Akhtar KL, Ebi KL, Hauengue M, <etal>et al<\/etal>.. (2007) Human Heath. In: Parry ML, Canziani OF, Palutikof JP, van der Linden PJ, Hanson CE, editors. Climate Change 2007: Impacts, adaptation and vulnerability. Contribution of working group II to the fourth assessment report of the Intergovernmental Panel on Climate Change. Cambridge, U.K.: Cambridge University Press. pp. 391\u2013431."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.2307\/3435012","article-title":"Climate variability and change in the United States: potential impacts on vector- and rodent-borne diseases","volume":"109 Suppl 2","author":"DJ Gubler","year":"2001","journal-title":"Environ Health Perspect"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.trstmh.2008.07.025","article-title":"Impact of climate change and other factors on emerging arbovirus diseases","volume":"103","author":"EA Gould","year":"2009","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref4","first-page":"383","article-title":"Climate change and mosquito-borne disease: knowing the horse before hitching the cart","volume":"27","author":"P Reiter","year":"2008","journal-title":"Rev Sci Tech"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1242\/jeb.037564","article-title":"Challenges in predicting climate and environmental effects on vector-borne disease episystems in a changing world","volume":"213","author":"WJ Tabachnick","year":"2010","journal-title":"J Exp Biol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1258\/ebm.2011.010402","article-title":"The role of climate variability and change in the transmission dynamics and geographic distribution of dengue","volume":"236","author":"KT Thai","year":"2011","journal-title":"Exp Biol Med (Maywood)"},{"key":"ref7","first-page":"163","article-title":"The effect of various temperatures in modifying the extrinsic incubation period of the yellow fever virus in <italic>Ae. aegypti<\/italic>","volume":"16","author":"NC Davis","year":"1932","journal-title":"Am J Hyg"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jmedent\/10.1.1","article-title":"The effect of environmental temperature upon the transmission of St. Louis encephalitis virus by <italic>Culex pipiens quinquefasciatus<\/italic>","volume":"10","author":"HS Hurlbut","year":"1973","journal-title":"J Med Entomol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"543","DOI":"10.4269\/ajtmh.1990.43.543","article-title":"Effect of environmental temperature on the vector competence of <italic>Aedes aegypti<\/italic> and <italic>Ae. taeniorhynchus<\/italic> for Ockelbo virus","volume":"43","author":"MJ Turell","year":"1990","journal-title":"Am J Trop Med Hyg"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"672","DOI":"10.4269\/ajtmh.1993.49.672","article-title":"Effect of environmental temperature on the vector competence of <italic>Aedes taeniorhynchus<\/italic> for Rift Valley fever and Venezuelan equine encephalitis viruses","volume":"49","author":"MJ Turell","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1089\/vbz.2009.0035","article-title":"Larval environmental temperature and the susceptibility of <italic>Aedes albopictus<\/italic> Skuse (Diptera: Culicidae) to chikungunya virus","volume":"10","author":"CJ Westbrook","year":"2009","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.4269\/ajtmh.1983.32.1130","article-title":"Effect of temperature of extrinsic incubation on the vector competence of <italic>Culex tarsalis<\/italic> for western equine encephalomyelitis virus","volume":"32","author":"LD Kramer","year":"1983","journal-title":"Am J Trop Med Hyg"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/jmedent\/35.3.289","article-title":"Characterization of modulation of western equine encephalomyelitis virus by <italic>Culex tarsalis<\/italic> (Diptera: Culicidae) maintained at 32 degrees C following parenteral infection","volume":"35","author":"LD Kramer","year":"1998","journal-title":"J Med Entomol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1603\/0022-2585-39.1.99","article-title":"Enhancement or modulation of the vector competence of <italic>Ochlerotatus vigilax<\/italic> (Diptera: Culicidae) for Ross River virus by temperature","volume":"39","author":"BH Kay","year":"2002","journal-title":"J Med Entomol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"963","DOI":"10.4269\/ajtmh.1980.29.963","article-title":"Effect of rearing temperature on transovarial transmission of St. Louis encephalitis virus in mosquitoes","volume":"29","author":"JL Hardy","year":"1980","journal-title":"Am J Trop Med Hyg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"19938","DOI":"10.1073\/pnas.0803408105","article-title":"Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes","volume":"105","author":"KM Myles","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1471-2180-8-47","article-title":"<italic>Aedes aegypti<\/italic> uses RNA interference in defense against Sindbis virus infection","volume":"8","author":"CL Campbell","year":"2008","journal-title":"BMC Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e1000299","DOI":"10.1371\/journal.ppat.1000299","article-title":"Dengue virus type 2 infections of <italic>Aedes aegypti<\/italic> are modulated by the mosquito&apos;s RNA interference pathway","volume":"5","author":"I Sanchez-Vargas","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e1002470","DOI":"10.1371\/journal.ppat.1002470","article-title":"Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma","volume":"8","author":"EM Morazzani","year":"2012","journal-title":"PLoS Pathog"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e30861","DOI":"10.1371\/journal.pone.0030861","article-title":"Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells","volume":"7","author":"N Vodovar","year":"2012","journal-title":"PLoS One"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1126\/science.1125694","article-title":"RNA interference directs innate immunity against viruses in adult Drosophila","volume":"312","author":"XH Wang","year":"2006","journal-title":"Science"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"19390","DOI":"10.1073\/pnas.1014378107","article-title":"RNAi-mediated immunity provides strong protection against the negative-strand RNA vesicular stomatitis virus in Drosophila","volume":"107","author":"S Mueller","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1038\/nri2824","article-title":"RNA-based antiviral immunity","volume":"10","author":"SW Ding","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.coi.2010.01.007","article-title":"Innate antiviral immunity in Drosophila","volume":"22","author":"LR Sabin","year":"2010","journal-title":"Curr Opin Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.ibmb.2008.04.002","article-title":"A transgenic sensor strain for monitoring the RNAi pathway in the yellow fever mosquito, <italic>Aedes aegypti<\/italic>","volume":"38","author":"ZN Adelman","year":"2008","journal-title":"Insect Biochem Mol Biol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.1128\/JVI.80.10.4992-4997.2006","article-title":"Effects of an Opal Termination Codon Preceding the nsP4 Gene Sequence in the O&apos;Nyong-Nyong Virus Genome on <italic>Anopheles gambiae<\/italic> Infectivity","volume":"80","author":"KM Myles","year":"2006","journal-title":"J Virol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1365-2583.2010.01005.x","article-title":"Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic <italic>Aedes aegypti<\/italic>","volume":"19","author":"MA Anderson","year":"2010","journal-title":"Insect Mol Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e7468","DOI":"10.1371\/journal.pone.0007468","article-title":"Accurate strand-specific quantification of viral RNA","volume":"4","author":"NE Plaskon","year":"2009","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"B Langmead","year":"2009","journal-title":"Genome Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.virol.2008.04.021","article-title":"West Nile virus infection of <italic>Drosophila melanogaster<\/italic> induces a protective RNAi response","volume":"377","author":"HL Chotkowski","year":"2008","journal-title":"Virology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1093\/emboj\/cdg74","article-title":"Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation","volume":"22","author":"G Szittya","year":"2003","journal-title":"Embo J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1104\/pp.105.066563","article-title":"Effect of temperature on geminivirus-induced RNA silencing in plants","volume":"138","author":"P Chellappan","year":"2005","journal-title":"Plant Physiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"143","DOI":"10.4269\/ajtmh.1987.36.143","article-title":"Effect of temperature on the vector efficiency of <italic>Aedes aegypti<\/italic> for dengue 2 virus","volume":"36","author":"DM Watts","year":"1987","journal-title":"Am J Trop Med Hyg"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1017\/S0950268809002052","article-title":"Assessing the effects of temperature on dengue transmission","volume":"137","author":"HM Yang","year":"2009","journal-title":"Epidemiol Infect"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e1470","DOI":"10.1371\/journal.pntd.0001470","article-title":"Climate-based models for understanding and forecasting dengue epidemics","volume":"6","author":"E Descloux","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1093\/jmedent\/43.3.484","article-title":"Ecological factors influencing <italic>Aedes aegypti<\/italic> (Diptera: Culicidae) productivity in artificial containers in Salinas, Puerto Rico","volume":"43","author":"R Barrera","year":"2006","journal-title":"J Med Entomol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1365-2915.2008.00783.x","article-title":"The effect of shade on the container index and pupal productivity of the mosquitoes <italic>Aedes aegypti<\/italic> and <italic>Culex pipiens<\/italic> breeding in artificial containers","volume":"23","author":"D Vezzani","year":"2009","journal-title":"Med Vet Entomol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1089\/vbz.2007.0649","article-title":"Geographic distribution and developmental sites of <italic>Aedes albopictus<\/italic> (Diptera: Culicidae) during a chikungunya epidemic event","volume":"8","author":"H Delatte","year":"2008","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"e201","DOI":"10.1371\/journal.ppat.0030201","article-title":"A single mutation in chikungunya virus affects vector specificity and epidemic potential","volume":"3","author":"KA Tsetsarkin","year":"2007","journal-title":"PLoS Pathog"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e1168","DOI":"10.1371\/journal.pone.0001168","article-title":"Two chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, <italic>Aedes albopictus<\/italic>","volume":"2","author":"M Vazeille","year":"2007","journal-title":"PLoS One"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1126\/science.272.5263.884","article-title":"Genetically engineered resistance to dengue-2 virus transmission in mosquitoes","volume":"272","author":"KE Olson","year":"1996","journal-title":"Science"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.1073\/pnas.0600479103","article-title":"Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified <italic>Aedes aegypti<\/italic>","volume":"103","author":"AW Franz","year":"2006","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0002239","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,11]],"date-time":"2024-05-11T06:58:24Z","timestamp":1715410704000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0002239"}},"subtitle":[],"editor":[{"given":"Michael J.","family":"Turell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,5,30]]},"references-count":42,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0002239","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,30]]}}}