{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T19:09:48Z","timestamp":1792868988422,"version":"4.5.0"},"reference-count":86,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T00:00:00Z","timestamp":1378944000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0002442","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T17:20:49Z","timestamp":1379006449000},"page":"e2442","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":173,"title":["Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex"],"prefix":"10.1371","volume":"7","author":[{"given":"Leijiane F.","family":"Sousa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina A.","family":"Nicolau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro S.","family":"Peixoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana L.","family":"Bernardoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e2mella S.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio","family":"Portes-Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa Helena V.","family":"Mour\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isa","family":"Lima-dos-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida S.","family":"Sano-Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hip\u00f3crates M.","family":"Chalkidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H.","family":"Valente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Moura-da-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,9,12]]},"reference":[{"key":"ref1","unstructured":"WHO (2007) Rabies and envenomings. A neglected public health issue. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/whqlibdoc.who.int\/publications\/2007\/9789241563482_eng.pdf\" xlink:type=\"simple\">http:\/\/whqlibdoc.who.int\/publications\/2007\/9789241563482_eng.pdf<\/ext-link>"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"e218","DOI":"10.1371\/journal.pmed.0050218","article-title":"The global burden of snakebite: a literature analysis and modelling based on regional estimates of envenoming and deaths","volume":"5","author":"A Kasturiratne","year":"2008","journal-title":"PLoS Med"},{"key":"ref3","unstructured":"Minist\u00e9rio-da-Sa\u00fade (2001) Manual de diagn\u00f3stico e tratamento de acidentes por animais pe\u00e7onhentos. 2nd ed. Bras\u00edlia: Funda\u00e7\u00e3o Nacional de Sa\u00fade."},{"key":"ref4","unstructured":"Brazil V (1918) Do envenenamento of\u00eddico e seu tratamento. Collectanea de Trabalhos (1901\u20131917) - Instituto Butantan. S\u00e3o Paulo: Typographia do Di\u00e1rio Official. pp. 31\u201352."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1002\/jms.1242","article-title":"Snake venomics. Strategy and applications","volume":"42","author":"JJ Calvete","year":"2007","journal-title":"J Mass Spectrom"},{"key":"ref6","article-title":"Snake venomics: From the inventory of toxins to biology","author":"JJ Calvete","year":"2013","journal-title":"Toxicon"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1586\/epr.11.61","article-title":"Proteomic tools against the neglected pathology of snake bite envenoming","volume":"8","author":"JJ Calvete","year":"2011","journal-title":"Expert Rev Proteomics"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/j.febslet.2009.03.029","article-title":"Venoms, venomics, antivenomics","volume":"583","author":"JJ Calvete","year":"2009","journal-title":"FEBS Lett"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jprot.2009.01.008","article-title":"Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming","volume":"72","author":"JM Guti\u00e9rrez","year":"2009","journal-title":"J Proteomics"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"369","DOI":"10.2174\/187152811797200669","article-title":"Antivenoms for snakebite envenomings","volume":"10","author":"JM Guti\u00e9rrez","year":"2011","journal-title":"Inflamm Allergy Drug Targets"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.toxicon.2012.04.342","article-title":"Second generation snake antivenomics: comparing immunoaffinity and immunodepletion protocols","volume":"60","author":"D Pla","year":"2012","journal-title":"Toxicon"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.4269\/ajtmh.2010.09-0733","article-title":"Antivenomic assessment of the immunological reactivity of EchiTAb-Plus-ICP, an antivenom for the treatment of snakebite envenoming in sub-Saharan Africa","volume":"82","author":"JJ Calvete","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3556","DOI":"10.1021\/pr800332p","article-title":"Snake venomics of the lancehead pitviper Bothrops asper: geographic, individual, and ontogenetic variations","volume":"7","author":"A Alape-Gir\u00f3n","year":"2008","journal-title":"J Proteome Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.toxicon.2009.06.011","article-title":"Studies on the venom proteome of Bothrops asper: perspectives and applications","volume":"54","author":"A Alape-Gir\u00f3n","year":"2009","journal-title":"Toxicon"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jprot.2009.01.005","article-title":"Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: Contributing to its taxonomy and snakebite management","volume":"72","author":"JJ Calvete","year":"2009","journal-title":"J Proteomics"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.jprot.2011.01.003","article-title":"Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management","volume":"74","author":"JJ Calvete","year":"2011","journal-title":"J Proteomics"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4396","DOI":"10.1021\/pr8003826","article-title":"Snake venomics of the Lesser Antillean pit vipers Bothrops caribbaeus and Bothrops lanceolatus: correlation with toxicological activities and immunoreactivity of a heterologous antivenom","volume":"7","author":"JM Guti\u00e9rrez","year":"2008","journal-title":"J Proteome Res"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jprot.2009.07.013","article-title":"Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Per\u00fa and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism","volume":"73","author":"V N\u00fa\u00f1ez","year":"2009","journal-title":"J Proteomics"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2707","DOI":"10.1016\/j.jprot.2012.03.028","article-title":"Combined snake venomics and venom gland transcriptomic analysis of Bothropoides pauloensis","volume":"75","author":"RS Rodrigues","year":"2012","journal-title":"J Proteomics"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.jprot.2008.07.007","article-title":"Snake venomics of the Brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy markers","volume":"71","author":"AK Tashima","year":"2008","journal-title":"J Proteomics"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1016\/j.toxicon.2006.01.022","article-title":"Comparison of indirect and direct approaches using ion-trap and Fourier transform ion cyclotron resonance mass spectrometry for exploring viperid venom proteomes","volume":"47","author":"JW Fox","year":"2006","journal-title":"Toxicon"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0003-9861(89)90350-0","article-title":"Degradation of extracellular matrix proteins by hemorrhagic metalloproteinases","volume":"275","author":"EN Baramova","year":"1989","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2422","DOI":"10.1021\/pr901128x","article-title":"The Venomics of Bothrops alternatus is a Pool of Acidic Proteins with Predominant Hemorrhagic and Coagulopathic Activities","volume":"9","author":"M Ohler","year":"2010","journal-title":"Journal of Proteome Research"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.jprot.2009.01.001","article-title":"Bothrops insularis venomics: a proteomic analysis supported by transcriptomic-generated sequence data","volume":"72","author":"RH Valente","year":"2009","journal-title":"J Proteomics"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"4218","DOI":"10.1002\/pmic.201100287","article-title":"Bothrops jararaca venom proteome rearrangement upon neonate to adult transition","volume":"11","author":"A Zelanis","year":"2011","journal-title":"Proteomics"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1016\/j.toxicon.2009.12.018","article-title":"Immunome and venome of Bothrops jararacussu: a proteomic approach to study the molecular immunology of snake toxins","volume":"55","author":"C Correa-Netto","year":"2010","journal-title":"Toxicon"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1016\/j.jprot.2012.01.020","article-title":"Exploring the proteomes of the venoms of the Peruvian pit vipers Bothrops atrox, B. barnetti and B. pictus","volume":"75","author":"M Kohlhoff","year":"2012","journal-title":"J Proteomics"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1186\/1471-2164-11-605","article-title":"A transcriptomic analysis of gene expression in the venom gland of the snake Bothrops alternatus (urutu)","volume":"11","author":"KC Cardoso","year":"2010","journal-title":"BMC Genomics"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.toxicon.2009.01.006","article-title":"Transcriptome analysis of the Amazonian viper Bothrops atrox venom gland using expressed sequence tags (ESTs)","volume":"53","author":"M Neiva","year":"2009","journal-title":"Toxicon"},{"key":"ref30","first-page":"211","article-title":"Analysis of Bothrops jararacussu venomous gland transcriptome focusing on structural and functional aspects: I\u2013gene expression profile of highly expressed phospholipases A2","volume":"86","author":"S Kashima","year":"2004","journal-title":"Biochimie"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.toxicon.2006.07.008","article-title":"Bothrops jararaca venom gland transcriptome: analysis of the gene expression pattern","volume":"48","author":"DA Cidade","year":"2006","journal-title":"Toxicon"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1186\/1471-2164-12-259","article-title":"Profiling the venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing","volume":"12","author":"J Durban","year":"2011","journal-title":"BMC Genomics"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.toxicon.2005.02.020","article-title":"Snake venom serine proteinases: sequence homology vs. substrate specificity, a paradox to be solved","volume":"45","author":"SM Serrano","year":"2005","journal-title":"Toxicon"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.toxicon.2012.09.006","article-title":"Phospholipases A(2): Unveiling the secrets of a functionally versatile group of snake venom toxins","volume":"62","author":"JM Guti\u00e9rrez","year":"2013","journal-title":"Toxicon"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.2174\/138161207782023711","article-title":"Importance of snake venom metalloproteinases in cell biology: Effects on platelets, inflammatory and endothelial cells","volume":"13","author":"AM Moura-da-Silva","year":"2007","journal-title":"Current Pharmaceutical Design"},{"key":"ref36","unstructured":"Tu AT (1977) Venoms: Chemistry and Molecular Biology. New York, USA: John Wiley."},{"key":"ref37","unstructured":"Tu AT (1991) Reptile Venoms and Toxins. Handbook of Natural Toxins. New York: Marcel Dekker, Inc."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1477-5956-4-11","article-title":"Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox","volume":"4","author":"RA Guercio","year":"2006","journal-title":"Proteome Sci"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0041-0101(03)00171-5","article-title":"Ontogenetic variability of Bothrops atrox and Bothrops asper snake venoms from Colombia","volume":"42","author":"MM Saldarriaga","year":"2003","journal-title":"Toxicon"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.toxicon.2005.11.007","article-title":"Sex-based individual variation of snake venom proteome among eighteen Bothrops jararaca siblings","volume":"47","author":"MC Menezes","year":"2006","journal-title":"Toxicon"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s00239-008-9186-1","article-title":"Snake population venomics: proteomics-based analyses of individual variation reveals significant gene regulation effects on venom protein expression in Sistrurus rattlesnakes","volume":"68","author":"HL Gibbs","year":"2009","journal-title":"J Mol Evol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/379537a0","article-title":"Diet and snake venom evolution","volume":"379","author":"JC Daltry","year":"1996","journal-title":"Nature"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/j.toxicon.2009.01.034","article-title":"Functional basis of a molecular adaptation: prey-specific toxic effects of venom from Sistrurus rattlesnakes","volume":"53","author":"HL Gibbs","year":"2009","journal-title":"Toxicon"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1098\/rspb.2009.0048","article-title":"Coevolution of diet and prey-specific venom activity supports the role of selection in snake venom evolution","volume":"276","author":"A Barlow","year":"2009","journal-title":"Proc Biol Sci"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.toxicon.2005.02.024","article-title":"Snake venom disintegrins: evolution of structure and function","volume":"45","author":"JJ Calvete","year":"2005","journal-title":"Toxicon"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/BF02338834","article-title":"Evolution of Disintegrin Cysteine-rich and mammalian Matrix-degrading Metalloproteinases: Gene duplication and divergence of a common ancestor rather than convergent evolution","volume":"43","author":"AM Moura-da-Silva","year":"1996","journal-title":"Journal of Molecular Evolution"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1463-6409.2011.00511.x","article-title":"Morphology, phylogeny and taxonomy of South American bothropoid pit vipers (Serpentes, Viperidae)","volume":"41","author":"PA Carrasco","year":"2012","journal-title":"Zoologica Scripta"},{"key":"ref48","unstructured":"W\u00fcster W, Salom\u00e3o MG, Quijada-Mascarenhas JA, Thorpe RS (2002) Origins and evolution of the South American pitvipers fauna: evidence from mitocondrial DNA sequence analysis. In: Shuett W, H\u00f6ggren M, Douglas ME, Greene HW, editors. Biology of the vipers. Eagle Mountain, UT: Eagle Mountain Publishing. pp. 111\u2013129."},{"key":"ref49","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ympev.2005.12.014","article-title":"Bayesian mixed models and the phylogeny of pitvipers (Viperidae: Serpentes)","volume":"39","author":"TA Castoe","year":"2006","journal-title":"Mol Phylogenet Evol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/j.1096-3642.2008.00495.x","article-title":"Morphological and molecular evidence for phylogeny and classification of South American pitvipers, genera <italic>Bothrops<\/italic>, <italic>Bothriopsis<\/italic>, and <italic>Bothrocophias<\/italic> (Serpentes: Viperidae)","volume":"156","author":"AM Fenwick","year":"2009","journal-title":"Zoological Journal of the Linnean Society"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.toxicon.2003.10.010","article-title":"Snake venom metalloproteinases: structure\/function relationships studies using monoclonal antibodies","volume":"42","author":"I Tanjoni","year":"2003","journal-title":"Toxicon"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.toxicon.2003.10.011","article-title":"Phylogenetic conservation of a snake venom metalloproteinase epitope recognized by a monoclonal antibody that neutralizes hemorrhagic activity","volume":"42","author":"I Tanjoni","year":"2003","journal-title":"Toxicon"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.biochi.2007.04.003","article-title":"Cytosolic glutathione peroxidase from liver of pacu (Piaractus mesopotamicus), a hypoxia-tolerant fish of the Pantanal","volume":"89","author":"VL Cunha Bastos","year":"2007","journal-title":"Biochimie"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1002\/rcm.1603","article-title":"MS1, MS2, and SQT-three unified, compact, and easily parsed file formats for the storage of shotgun proteomic spectra and identifications","volume":"18","author":"WH McDonald","year":"2004","journal-title":"Rapid Commun Mass Spectrom"},{"key":"ref55","first-page":"S","article-title":"ProLuCID, a fast and sensitive tandem mass spectra-based protein identification program","volume":"5","author":"T Xu","year":"2006","journal-title":"Molecular and Cellular Proteomics"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.1021\/ac025747h","article-title":"Empirical statistical model to estimate the accuracy of peptide identifications made by MS\/MS and database search","volume":"74","author":"A Keller","year":"2002","journal-title":"Anal Chem"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1021\/ac0341261","article-title":"A statistical model for identifying proteins by tandem mass spectrometry","volume":"75","author":"AI Nesvizhskii","year":"2003","journal-title":"Anal Chem"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0041-0101(01)00245-8","article-title":"Pharmacological characterization and neutralization of the venoms used in the production of Bothropic antivenom in Brazil","volume":"40","author":"KU Camey","year":"2002","journal-title":"Toxicon"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0041-0101(89)90096-2","article-title":"Comparative study on coagulant, defibrinating, fibrinolytic and fibrinogenolytic activities of Costa Rican crotaline snake venoms and their neutralization by a polyvalent antivenom","volume":"27","author":"JA Gen\u00e9","year":"1989","journal-title":"Toxicon"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/0041-0101(90)90094-N","article-title":"Differences in distribution of myotoxic proteins in venoms from different Bothrops species","volume":"28","author":"AM Moura-da-Silva","year":"1990","journal-title":"Toxicon"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/0041-0101(91)90063-W","article-title":"Isolation and Comparison of Myotoxins Isolated From Venoms of Different Species of <italic>Bothrops<\/italic> Snakes","volume":"29","author":"AM Moura-da-Silva","year":"1991","journal-title":"Toxicon"},{"key":"ref62","unstructured":"Mackessy SP (2008) Venom composition in rattlesnakes: trends and biological significance. In: Hayes WK, Beaman KR, Cardwell MD, Bush SP, editors. The Biology of Rattlesnakes. Loma Linda, CA, USA : Loma Linda University Press. pp. 495\u2013510."},{"key":"ref63","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1021\/pr700610z","article-title":"Snake venomics of Central American pitvipers: clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi","volume":"7","author":"Y Angulo","year":"2008","journal-title":"J Proteome Res"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"4234","DOI":"10.1021\/pr100545d","article-title":"Snake venomics of Bothriechis nigroviridis reveals extreme variability among palm pitviper venoms: different evolutionary solutions for the same trophic purpose","volume":"9","author":"J Fern\u00e1ndez","year":"2010","journal-title":"J Proteome Res"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"e67220","DOI":"10.1371\/journal.pone.0067220","article-title":"Phylogeny-Based Comparative Analysis of Venom Proteome Variation in a Clade of Rattlesnakes (Sistrurus sp.)","volume":"8","author":"HL Gibbs","year":"2013","journal-title":"PLoS One"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/j.toxicon.2010.02.028","article-title":"Evolutionary trends in venom composition in the western rattlesnakes (Crotalus viridis sensu lato): toxicity vs. tenderizers","volume":"55","author":"SP Mackessy","year":"2010","journal-title":"Toxicon"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1021\/pr9008749","article-title":"Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America","volume":"9","author":"JJ Calvete","year":"2010","journal-title":"J Proteome Res"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1111\/j.1742-4658.2008.06466.x","article-title":"Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity","volume":"275","author":"JW Fox","year":"2008","journal-title":"Febs Journal"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"39746","DOI":"10.1074\/jbc.M604855200","article-title":"The cysteine-rich domain of snake venom metalloproteinases is a ligand for von Willebrand factor A domains: role in substrate targeting","volume":"281","author":"SM Serrano","year":"2006","journal-title":"Journal of Biological Chemistry"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"e727","DOI":"10.1371\/journal.pntd.0000727","article-title":"Mechanisms of Vascular Damage by Hemorrhagic Snake Venom Metalloproteinases: Tissue Distribution and In Situ Hydrolysis","volume":"4","author":"C Baldo","year":"2010","journal-title":"Plos Neglected Tropical Diseases"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0300-9084(00)01163-9","article-title":"Snake venom metalloproteinases: Their role in the pathogenesis of local tissue damage","volume":"82","author":"JM Gutierrez","year":"2000","journal-title":"Biochimie"},{"key":"ref72","first-page":"589","article-title":"Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains","volume":"386","author":"JC Modesto","year":"2005","journal-title":"Biol Chem"},{"key":"ref73","first-page":"315","article-title":"Randomized comparative trial of three antivenoms in the treatment of envenoming by lance-headed vipers (Bothrops jararaca) in S\u00e3o Paulo, Brazil","volume":"86","author":"JL Cardoso","year":"1993","journal-title":"Quarterly Journal of Medicine"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/S0041-0101(98)00145-7","article-title":"Neutralization of local tissue damage induced by Bothrops asper (terciopelo) snake venom","volume":"36","author":"JM Gutierrez","year":"1998","journal-title":"Toxicon"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3181\/00379727-219-44322","article-title":"Viper venom disintegrins and related molecules","volume":"219","author":"MA McLane","year":"1998","journal-title":"Proceedings of the Society for Experimental Biology and Medicine"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/j.toxicon.2009.12.014","article-title":"Antigenic cross-reactivity and immunogenicity of Bothrops venoms from snakes of the Amazon region","volume":"55","author":"MeF Furtado","year":"2010","journal-title":"Toxicon"},{"key":"ref77","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/j.toxicon.2008.10.002","article-title":"Interspecific variation in venom composition and toxicity of Brazilian snakes from Bothrops genus","volume":"52","author":"GP Queiroz","year":"2008","journal-title":"Toxicon"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1016\/S0041-0101(00)00082-9","article-title":"Neutralizing potency of horse antibothropic Brazilian antivenom against Bothrops snake venoms from the Amazonian rain forest","volume":"38","author":"EG Muniz","year":"2000","journal-title":"Toxicon"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.toxicon.2010.07.001","article-title":"Preclinical assessment of the neutralizing capacity of antivenoms produced in six Latin American countries against medically-relevant Bothrops snake venoms","volume":"56","author":"A Segura","year":"2010","journal-title":"Toxicon"},{"key":"ref80","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0041-0101(00)00122-7","article-title":"The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms","volume":"39","author":"P Saravia","year":"2001","journal-title":"Toxicon"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/S0035-9203(03)00005-1","article-title":"Clinical trial of two antivenoms for the treatment of Bothrops and Lachesis bites in the north eastern Amazon region of Brazil","volume":"98","author":"PPO Pardal","year":"2004","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"ref82","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1016\/j.toxicon.2009.12.015","article-title":"Antivenomics and venom phenotyping: A marriage of convenience to address the performance and range of clinical use of antivenoms","volume":"56","author":"JJ Calvete","year":"2010","journal-title":"Toxicon"},{"key":"ref83","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1016\/j.jprot.2011.07.004","article-title":"Omic technologies to fight the neglect","volume":"74","author":"JJ Calvete","year":"2011","journal-title":"J Proteomics"},{"key":"ref84","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.toxicon.2012.11.016","article-title":"Assessing the preclinical efficacy of antivenoms: From the lethality neutralization assay to antivenomics","volume":"69","author":"JM Guti\u00e9rrez","year":"2013","journal-title":"Toxicon"},{"key":"ref85","doi-asserted-by":"crossref","first-page":"2637","DOI":"10.1093\/molbev\/msr091","article-title":"Domain loss facilitates accelerated evolution and neofunctionalization of duplicate snake venom metalloproteinase toxin genes","volume":"28","author":"NR Casewell","year":"2011","journal-title":"Mol Biol Evol"},{"key":"ref86","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jprot.2009.01.009","article-title":"Evolution and diversification of the Toxicofera reptile venom system","volume":"72","author":"BG Fry","year":"2009","journal-title":"J Proteomics"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0002442","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T12:22:57Z","timestamp":1540210977000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0002442"}},"subtitle":[],"editor":[{"given":"Jos\u00e9","family":"Guti\u00e9rrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,9,12]]},"references-count":86,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2013,9,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0002442","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,12]]}}}