{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:14:06Z","timestamp":1793661246657,"version":"4.5.1"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0002504","type":"journal-article","created":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T21:17:28Z","timestamp":1382044648000},"page":"e2504","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":39,"title":["Diagnostic Accuracy of Loopamp Trypanosoma brucei Detection Kit for Diagnosis of Human African Trypanosomiasis in Clinical Samples"],"prefix":"10.1371","volume":"7","author":[{"given":"Patrick","family":"Mitashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Epco","family":"Hasker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieudonn\u00e9 Mumba","family":"Ngoyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pati Patient","family":"Pyana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veerle","family":"Lejon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Van der Veken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Lutumba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"B\u00fcscher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen","family":"Boelaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stijn","family":"Deborggraeve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,10,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"e55","DOI":"10.1371\/journal.pmed.0050055","article-title":"Eliminating human African trypanosomiasis: where do we stand and what comes next?","volume":"5","author":"PP Simarro","year":"2008","journal-title":"PLoS Med"},{"key":"ref2","unstructured":"WHO (2012) Trypanosomiasis, Human African (sleeping sickness). Available from:<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/mediacentre\/factsheets\/fs259\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/mediacentre\/factsheets\/fs259\/en\/<\/ext-link>. Accessed:21 March 2013. Fact sheet 259. 10-1-2012."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1128\/CMR.18.1.133-146.2005","article-title":"Options for field diagnosis of human african trypanosomiasis","volume":"18","author":"F Chappuis","year":"2005","journal-title":"Clin Microbiol Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e471","DOI":"10.1371\/journal.pntd.0000471","article-title":"Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for sleeping sickness diagnosis and staging","volume":"3","author":"P Buscher","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1111\/j.1365-3156.2010.02546.x","article-title":"Sleeping sickness diagnosis: use of buffy coats improves the sensitivity of the mini anion exchange centrifugation test","volume":"15","author":"M Camara","year":"2010","journal-title":"Trop Med Int Health"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1586\/erm.12.72","article-title":"Recent progress in molecular diagnosis of sleeping sickness","volume":"12","author":"S Deborggraeve","year":"2012","journal-title":"Expert Rev Mol Diagn"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e1438","DOI":"10.1371\/journal.pntd.0001438","article-title":"Diagnostic accuracy of molecular amplification tests for human African trypanosomiasis\u2013systematic review","volume":"6","author":"CM Mugasa","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"e1919","DOI":"10.1371\/journal.pntd.0001919","article-title":"Human african trypanosomiasis diagnosis in first-line health services of endemic countries, a systematic review","volume":"6","author":"P Mitashi","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e1572","DOI":"10.1371\/journal.pntd.0001572","article-title":"Loop-mediated isothermal amplification technology: towards point of care diagnostics","volume":"6","author":"ZK Njiru","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e865","DOI":"10.1371\/journal.pntd.0000865","article-title":"LAMP for human African trypanosomiasis: a comparative study of detection formats","volume":"4","author":"SL Wastling","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e1950","DOI":"10.1371\/journal.pntd.0001950","article-title":"Human african trypanosomiasis in the democratic republic of the congo: a looming emergency?","volume":"6","author":"E Hasker","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref12","first-page":"384","article-title":"The haematocrit centrifuge technique for the diagnosis of African trypanosomiasis","volume":"27","author":"PT Woo","year":"1970","journal-title":"Acta Trop"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e972","DOI":"10.1371\/journal.pntd.0000972","article-title":"Diagnostic accuracy of PCR in gambiense sleeping sickness diagnosis, staging and post-treatment follow-up: a 2-year longitudinal study","volume":"5","author":"S Deborggraeve","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e2180","DOI":"10.1371\/journal.pntd.0002180","article-title":"False positivity of non-targeted infections in malaria rapid diagnostic tests: the case of human african trypanosomiasis","volume":"7","author":"P Gillet","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2307\/2529786","article-title":"An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers","volume":"33","author":"JR Landis","year":"1977","journal-title":"Biometrics"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1128\/JCM.01817-10","article-title":"Detection of Group 1 Trypanosoma brucei gambiense by loop-mediated isothermal amplification","volume":"49","author":"ZK Njiru","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.1128\/JCM.00259-09","article-title":"Leishmania OligoC-TesT as a simple, rapid, and standardized tool for molecular diagnosis of cutaneous leishmaniasis in Peru","volume":"47","author":"D Espinosa","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0001-706X(92)90054-2","article-title":"Absence of the LiTat 1.3 (CATT antigen) gene in Trypanosoma brucei gambiense stocks from Cameroon","volume":"51","author":"P Dukes","year":"1992","journal-title":"Acta Trop"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1080\/00034983.1998.11813349","article-title":"Parasitological detection of Trypanosoma brucei gambiense in serologically negative sleeping-sickness suspects from north-western Uganda","volume":"92","author":"JC Enyaru","year":"1998","journal-title":"Ann Trop Med Parasitol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0001-706X(02)00029-3","article-title":"Comparison of different DNA preparation protocols for PCR diagnosis of Human African Trypanosomosis in Cote d&apos;Ivoire","volume":"82","author":"P Solano","year":"2002","journal-title":"Acta Trop"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.diagmicrobio.2008.03.006","article-title":"Molecular analysis of archived blood slides reveals an atypical human Trypanosoma infection","volume":"61","author":"S Deborggraeve","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e1007","DOI":"10.1371\/journal.pntd.0001007","article-title":"The human African trypanosomiasis control and surveillance programme of the World Health Organization 2000\u20132009: the way forward","volume":"5","author":"PP Simarro","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1056\/NEJMc1210373","article-title":"Rapid diagnostic test for sleeping sickness","volume":"368","author":"P Buscher","year":"2013","journal-title":"N Engl J Med"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0002504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T19:37:34Z","timestamp":1539977854000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0002504"}},"subtitle":[],"editor":[{"given":"Joseph Mathu","family":"Ndung'u","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,17]]},"references-count":23,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0002504","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,17]]}}}