{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:56:53Z","timestamp":1787021813929,"version":"build-2736575974"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T00:00:00Z","timestamp":1402531200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0002860","type":"journal-article","created":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T18:52:16Z","timestamp":1402599136000},"page":"e2860","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Comparative Assessment of ELISAs Using Recombinant Saposin-Like Protein 2 and recombinant Cathepsin L-1 from Fasciola hepatica for the Serodiagnosis of Human Fasciolosis"],"prefix":"10.1371","volume":"8","author":[{"given":"Bruno","family":"Gottstein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Schneeberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghalia","family":"Boubaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette","family":"Merkle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Huber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Spiliotis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Garate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus G.","family":"Doherr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1136\/bmj.288.6425.1197","article-title":"Hydatid false positive serological test results in malignancy","volume":"288","author":"FK Dar","year":"1984","journal-title":"Brit Med J"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1111\/j.1469-0691.1999.tb00515.x","article-title":"Immunodiagnosis of echinococcosis in cancer patients","volume":"5","author":"M Pfister","year":"1999","journal-title":"Clin Microbiol Infect"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"193","DOI":"10.4269\/ajtmh.1999.60.193","article-title":"Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies","volume":"60","author":"D Poretti","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1423-0410.1980.tb02345.x","article-title":"Comparative automated assay of anti-P1 antibodies in acute hepatic distomiasis (fascioliasis) and in hydatidosis (1980)","volume":"38","author":"R Ben-Ismail","journal-title":"Vox Sang"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/00034983.1992.11812635","article-title":"Immunological localization of blood P1 antigen is tissues of <italic>Echinococcus granulosus<\/italic>","volume":"86","author":"S Makni","year":"1992","journal-title":"Ann Trop Med Parasitol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1017\/S0031182006002083","article-title":"Improved serodiagnosis of alveolar echinococcosis of humans using an <italic>in vitro<\/italic>-produced <italic>Echinococcus multilocularis<\/italic> antigen","volume":"134","author":"N Mueller","year":"2007","journal-title":"Parasitology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"188","DOI":"10.4269\/ajtmh.1999.60.188","article-title":"Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of <italic>Echinococcus granulosus<\/italic> cyst fluid (antigen B) and <italic>E. multilo<\/italic>cularis protoscolex (Em18)","volume":"60","author":"A Ito","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1128\/JCM.29.9.1831-1835.1991","article-title":"Immunodiagnosis of toxocarosis in humans: evaluation of a new ELISA test kit","volume":"29","author":"P Jacquier","year":"1991","journal-title":"J Clin Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1128\/CVI.00257-08","article-title":"Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis","volume":"15","author":"MA G\u00f3mez-Morales","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1007\/s10096-011-1170-9","article-title":"<italic>Toxocara<\/italic> and <italic>Ascaris<\/italic> seropositivity among patients suspected of visceral and ocular larva migrans in the Netherlands: trends from 1998 to 2009","volume":"30","author":"E Pinelli","year":"2011","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"977","DOI":"10.4269\/ajtmh.2007.76.977","article-title":"Evaluation of Fas2-ELISA for the serological detection of <italic>Fasciola hepatica<\/italic> infection in humans","volume":"76","author":"JR Espinoza","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1111\/j.1365-3156.2012.02964.x","article-title":"Assessing the validity of an ELISA test for the serological diagnosis of human fascioliasis in different epidemiological situations","volume":"17","author":"MA Valero","year":"2012","journal-title":"Trop Med Int Health"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"977","DOI":"10.4269\/ajtmh.2007.76.977","article-title":"Evaluation of Fas2-ELISA for the serological detection of <italic>Fasciola hepatica<\/italic> infection in humans","volume":"76","author":"JR Espinoza","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1645\/GE-3113","article-title":"Molecular cloning of a member of the saposin-like protein family","volume":"89","author":"AM Espino","year":"2003","journal-title":"J Parasitol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.diagmicrobio.2011.03.016","article-title":"<italic>Fasciola hepatica<\/italic> saposin-like protein-2\u2013based ELISA for the serodiagnosis of chronic human fascioliasis","volume":"70","author":"O Figueroa-Santiago","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"749","DOI":"10.4269\/ajtmh.1999.60.749","article-title":"Short report: immunodiagnosis of human fascioliasis using recombinant <italic>Fasciola hepatica<\/italic> cathepsin L1 cysteine proteinase","volume":"60","author":"SM O'Neill","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1515\/CCLM.2001.088","article-title":"Elevated carbohydrate antigen 19-9 in patients with <italic>Echinococcus<\/italic> infection","volume":"39","author":"M Pfister","year":"2001","journal-title":"Clin Chem Lab Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.amjsurg.2007.02.024","article-title":"Serum tumor marker CA19-9 in the follow-up of patients with cystic echinococcosis","volume":"195","author":"BC Yuksel","year":"2008","journal-title":"Am J Surg"},{"key":"ref19","first-page":"904","article-title":"Erkrankungen bei Menschen durch Befall mit dem grossen Leberegel (<italic>Fasciola hepatica<\/italic>)","volume":"49","year":"2009","journal-title":"FOPH Bulletin"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"605","DOI":"10.4269\/ajtmh.1987.37.605","article-title":"Immunodiagnosis of human fascioliasis by enzyme-linked immunosorbent assay using excretory-secretory products","volume":"37","author":"AM Espino","year":"1987","journal-title":"Am J Trop Med Hyg"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"308","DOI":"10.4269\/ajtmh.1986.35.308","article-title":"Demonstration of species-specific and cross-reactive components of <italic>Taenia solium<\/italic> metacestode antigens","volume":"35","author":"B Gottstein","year":"1986","journal-title":"Am J Trop Med Hyg"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.trstmh.2008.09.020","article-title":"Isolation and molecular characterization of recombinant <italic>Echinococcus granulosus<\/italic> P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients","volume":"103","author":"N Ben Nouir","year":"2009","journal-title":"Trans Roy Soc Trop Med Hyg"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1128\/JCM.31.2.373-376.1993","article-title":"Imporoved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus-antigen","volume":"31","author":"B Gottstein","year":"1993","journal-title":"J Clin Microbiol"},{"key":"ref24","unstructured":"Hillyer GV (1999) Immunodiagnosis of human and animal fasciolosis. In: Dalton JP, editor. Fasciolosis. Oxon, UK: CABI Publishing, pp. 435\u2013447."},{"key":"ref25","unstructured":"Mas-Coma S, Bargues MD, Esteban JG (1999) Human fasciolosis. In: Dalton JP, editor. Fasciolosis. Oxon, UK: CABI Publishing, pp. 411\u2013434."},{"key":"ref26","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0732-8893(01)00288-7","article-title":"Immunodiagnosis of fasciolosis using recombinant procathepsin L cysteine proteinase","volume":"41","author":"S Carnevale","year":"2001","journal-title":"Diagn Microbiol Inf Dis"},{"issue":"(9)","key":"ref27","doi-asserted-by":"crossref","first-page":"e2414","DOI":"10.1371\/journal.pntd.0002414","article-title":"The diagnosis of human fasciolosis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease","volume":"7","author":"B Gonzales Santana","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1017\/S0031182007003435","article-title":"Evaluation of immunoglobulin G4 subclass antibody in a peptide-based enzyme-linked immunosorbent assay for the serodiagnosis of human fascioliasis","volume":"134","author":"C Tantrawatpan","year":"2007","journal-title":"Parasitology"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0002860","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T19:19:26Z","timestamp":1597951166000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0002860"}},"subtitle":[],"editor":[{"given":"Patrick J.","family":"Lammie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,12]]},"references-count":28,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0002860","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,12]]}}}