{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T22:11:30Z","timestamp":1781561490604,"version":"3.54.5"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T00:00:00Z","timestamp":1417651200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0003317","type":"journal-article","created":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T19:37:04Z","timestamp":1417721824000},"page":"e3317","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Therapeutic Administration of Recombinant Paracoccin Confers Protection against Paracoccidioides brasiliensis Infection: Involvement of TLRs"],"prefix":"10.1371","volume":"8","author":[{"given":"Ana Claudia Paiva","family":"Alegre-Maller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Costa","family":"Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Aparecido","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Ferreira","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Silveira","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebert Seixas","family":"Hanna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor C.","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Gay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Roque-Barreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,12,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.clindermatol.2012.01.006","article-title":"Paracoccidioidomycosis","volume":"30","author":"SA Marques","year":"2012","journal-title":"Clin Dermatol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/CMR.6.2.89","article-title":"Paracoccidioidomycosis: an update","volume":"6","author":"E Brummer","year":"1993","journal-title":"Clin Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0966-842X(01)02292-2","article-title":"The pathobiology of Paracoccidioides brasiliensis","volume":"10","author":"MI Borges-Walmsley","year":"2002","journal-title":"Trends Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1006\/cyto.2000.0824","article-title":"Imbalance of IL-2, IFN-gamma and IL-10 secretion in the immunosuppression associated with human paracoccidioidomycosis","volume":"13","author":"G Benard","year":"2001","journal-title":"Cytokine"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1590\/S0100-879X1998000500003","article-title":"Cytokines produced by susceptible and resistant mice in the course of Paracoccidioides brasiliensis infection","volume":"31","author":"VL Calich","year":"1998","journal-title":"Braz J Med Biol Res"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0923-2494(98)80764-5","article-title":"Immunity to Paracoccidioides brasiliensis infection","volume":"149","author":"VL Calich","year":"1998","journal-title":"Res Immunol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1128\/IAI.66.2.800-806.1998","article-title":"Protective role of gamma interferon in experimental pulmonary paracoccidioidomycosis","volume":"66","author":"LE Cano","year":"1998","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S1286-4579(03)00040-6","article-title":"Production of pro- and anti-inflammatory cytokines by monocytes from patients with paracoccidioidomycosis","volume":"5","author":"MT Peracoli","year":"2003","journal-title":"Microbes Infect"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1016\/S0002-9440(10)65053-5","article-title":"Interferon-gamma and tumor necrosis factor-alpha determine resistance to Paracoccidioides brasiliensis infection in mice","volume":"156","author":"JT Souto","year":"2000","journal-title":"Am J Pathol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1080\/13693780801982762","article-title":"Deficiency of IL-12p40 subunit determines severe paracoccidioidomycosis in mice","volume":"46","author":"MC Livonesi","year":"2008","journal-title":"Med Mycol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1080\/mmy.38.s1.323.333","article-title":"Antifungal compounds: controversies, queries and conclusions","volume":"38","author":"JR Graybill","year":"2000","journal-title":"Med Mycol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1517\/14656566.6.13.2231","article-title":"Drug-drug interactions of antifungal agents and implications for patient care","volume":"6","author":"PO Gubbins","year":"2005","journal-title":"Expert Opin Pharmacother"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nri1255","article-title":"Immunity to fungal infections","volume":"4","author":"L Romani","year":"2004","journal-title":"Nat Rev Immunol"},{"key":"ref14","first-page":"218","article-title":"ArtinM offers new perspectives in the development of antifungal therapy","volume":"3","author":"LP Ruas","year":"2012","journal-title":"Front Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"5461","DOI":"10.1016\/j.vaccine.2008.07.097","article-title":"Protective immunity induced by rPb27 of Paracoccidioides brasiliensis","volume":"26","author":"BS Reis","year":"2008","journal-title":"Vaccine"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/j.micinf.2011.06.004","article-title":"The combined use of Paracoccidioides brasiliensis Pb40 and Pb27 recombinant proteins enhances chemotherapy effects in experimental paracoccidioidomycosis","volume":"13","author":"VC Fernandes","year":"2011","journal-title":"Microbes Infect"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.4161\/hv.21283","article-title":"Paracoccidioidomycosis vaccine","volume":"8","author":"LR Travassos","year":"2012","journal-title":"Hum Vaccin Immunother"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"7893","DOI":"10.1016\/j.vaccine.2007.09.011","article-title":"Immunization with radioattenuated yeast cells of Paracoccidioides brasiliensis induces a long lasting protection in BALB\/c mice","volume":"25","author":"EM do Nascimento Martins","year":"2007","journal-title":"Vaccine"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e1519","DOI":"10.1371\/journal.pntd.0001519","article-title":"Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis","volume":"6","author":"GM Rittner","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e2788","DOI":"10.1371\/journal.pntd.0002788","article-title":"Recombinant paracoccin reproduces the biological properties of the native protein and induces protective Th1 immunity against Paracoccidioides brasiliensis infection","volume":"8","author":"AC Alegre","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0090-1229(79)90108-9","article-title":"The activation of the complement system by Paracoccidioides brasiliensis in vitro: its opsonic effect and possible significance for an in vivo model of infection","volume":"12","author":"VL Calich","year":"1979","journal-title":"Clin Immunol Immunopathol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","article-title":"Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids","volume":"126","author":"LC Green","year":"1982","journal-title":"Anal Biochem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e98512","DOI":"10.1371\/journal.pone.0098512","article-title":"Recognition of TLR2 N-Glycans: Critical Role in ArtinM Immunomodulatory Activity","volume":"9","author":"VS Mariano","year":"2014","journal-title":"PLoS One"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"34589","DOI":"10.1074\/jbc.M403830200","article-title":"Four N-linked glycosylation sites in human toll-like receptor 2 cooperate to direct efficient biosynthesis and secretion","volume":"279","author":"AN Weber","year":"2004","journal-title":"The Journal of biological chemistry"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"37561","DOI":"10.1074\/jbc.M306137200","article-title":"The antifungal drug amphotericin B promotes inflammatory cytokine release by a Toll-like receptor- and CD14-dependent mechanism","volume":"278","author":"K Sau","year":"2003","journal-title":"J Biol Chem"},{"key":"ref26","article-title":"The immunomodulatory effect of plant lectins: a review with emphasis on ArtinM properties","author":"MA Souza","year":"2013","journal-title":"Glycoconj J"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1016\/j.bcp.2011.03.010","article-title":"Plant lectins are novel Toll-like receptor agonists","volume":"81","author":"J Unitt","year":"2011","journal-title":"Biochem Pharmacol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3389\/fmicb.2014.00020","article-title":"Plant lectins and Toll-like receptors: implications for therapy of microbial infections","volume":"5","author":"LC da Silva","year":"2014","journal-title":"Front Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.4049\/jimmunol.153.4.1798","article-title":"A neutrophil migration-inducing lectin from Artocarpus integrifolia","volume":"153","author":"R Santos-de-Oliveira","year":"1994","journal-title":"J Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1023\/A:1006999323098","article-title":"Neutrophil haptotaxis induced by the lectin KM+","volume":"15","author":"L Ganiko","year":"1998","journal-title":"Glycoconj J"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1093\/glycob\/11.12.1035","article-title":"KM(+), a lectin from Artocarpus integrifolia, induces IL-12 p40 production by macrophages and switches from type 2 to type 1 cell-mediated immunity against Leishmania major antigens, resulting in BALB\/c mice resistance to infection","volume":"11","author":"A Panunto-Castelo","year":"2001","journal-title":"Glycobiology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.bbagen.2004.10.012","article-title":"Lectin KM+-induced neutrophil haptotaxis involves binding to laminin","volume":"1721","author":"L Ganiko","year":"2005","journal-title":"Biochim Biophys Acta"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.bbagen.2005.09.011","article-title":"Neutrophil activation induced by the lectin KM+ involves binding to CXCR2","volume":"1760","author":"G Pereira-da-Silva","year":"2006","journal-title":"Biochim Biophys Acta"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"423","DOI":"10.2353\/ajpath.2008.080126","article-title":"Therapeutic administration of KM+ lectin protects mice against Paracoccidioides brasiliensis infection via interleukin-12 production in a toll-like receptor 2-dependent mechanism","volume":"173","author":"KC Coltri","year":"2008","journal-title":"Am J Pathol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1023\/A:1006947306260","article-title":"An intravascular chemoattractant lectin inhibits neutrophil migration","volume":"15","author":"M Sakamoto","year":"1998","journal-title":"Glycoconj J"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"3001","DOI":"10.1016\/j.vaccine.2005.11.067","article-title":"Potential of KM+ lectin in immunization against Leishmania amazonensis infection","volume":"24","author":"CR Teixeira","year":"2006","journal-title":"Vaccine"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.imlet.2009.01.009","article-title":"Neutrophil activation induced by ArtinM: release of inflammatory mediators and enhancement of effector functions","volume":"123","author":"KA Toledo","year":"2009","journal-title":"Immunol Lett"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e9776","DOI":"10.1371\/journal.pone.0009776","article-title":"The lectin ArtinM induces recruitment of rat mast cells from the bone marrow to the peritoneal cavity","volume":"5","author":"PA de Almeida Buranello","year":"2010","journal-title":"PloS one"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.bbrc.2011.11.033","article-title":"The lectin ArtinM binds to mast cells inducing cell activation and mediator release","volume":"416","author":"VC Barbosa-Lorenzi","year":"2011","journal-title":"Biochem Biophys Res Commun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"433","DOI":"10.2174\/187152812803589985","article-title":"Neutrophil activation induced by plant lectins: modulation of inflammatory processes","volume":"11","author":"G Pereira-da-Silva","year":"2012","journal-title":"Inflamm Allergy Drug Targets"},{"key":"ref41","article-title":"Activation of spleen cells by ArtinM may account for its immunomodulatory properties","author":"TA da Silva","year":"2014","journal-title":"Cell Tissue Res"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"9183","DOI":"10.1016\/j.vaccine.2011.09.136","article-title":"ArtinM, a D-mannose-binding lectin from Artocarpus integrifolia, plays a potent adjuvant and immunostimulatory role in immunization against Neospora caninum","volume":"29","author":"MR Cardoso","year":"2011","journal-title":"Vaccine"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.intimp.2011.05.005","article-title":"Protective effect of Artin M from extract of Artocarpus integrifolia seeds by Th1 and Th17 immune response on the course of infection by Candida albicans","volume":"11","author":"LA Custodio","year":"2011","journal-title":"Int Immunopharmacol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"792","DOI":"10.3109\/13693780903501671","article-title":"Protection against Paracoccidioides brasiliensis infection conferred by the prophylactic administration of native and recombinant ArtinM","volume":"48","author":"KC Coltri","year":"2010","journal-title":"Med Mycol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.micinf.2005.09.008","article-title":"Paracoccin, a GlcNAc-binding lectin from Paracoccidioides brasiliensis, binds to laminin and induces TNF-alpha production by macrophages","volume":"8","author":"KC Coltri","year":"2006","journal-title":"Microbes Infect"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1111\/j.1365-2133.2006.07161.x","article-title":"Cytokines released from blood monocytes and expressed in mucocutaneous lesions of patients with paracoccidioidomycosis evaluated before and during trimethoprim-sulfamethoxazole treatment","volume":"154","author":"MR Parise-Fortes","year":"2006","journal-title":"Br J Dermatol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s11046-007-9065-0","article-title":"An overview of the immunopathology of human paracoccidioidomycosis","volume":"165","author":"G Benard","year":"2008","journal-title":"Mycopathologia"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S1286-4579(02)00078-3","article-title":"Effect of cytokines on the in vitro fungicidal activity of monocytes from paracoccidioidomycosis patients","volume":"5","author":"SA Calvi","year":"2003","journal-title":"Microbes Infect"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1006\/cyto.2002.0884","article-title":"IL-12 and neutralization of endogenous IL-10 revert the in vitro antigen-specific cellular immunosuppression of paracoccidioidomycosis patients","volume":"18","author":"CC Romano","year":"2002","journal-title":"Cytokine"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"S55","DOI":"10.1016\/S0264-410X(03)00201-9","article-title":"The role of Toll-like receptor 2 in microbial disease and immunity","volume":"21","author":"LM Wetzler","year":"2003","journal-title":"Vaccine"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1093\/intimm\/13.7.933","article-title":"Discrimination of bacterial lipoproteins by Toll-like receptor 6","volume":"13","author":"O Takeuchi","year":"2001","journal-title":"Int Immunol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4049\/jimmunol.169.1.10","article-title":"Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins","volume":"169","author":"O Takeuchi","year":"2002","journal-title":"J Immunol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1074\/jbc.M109910200","article-title":"MD-2 and TLR4 N-linked glycosylations are important for a functional lipopolysaccharide receptor","volume":"277","author":"J da Silva Correia","year":"2002","journal-title":"J Biol Chem"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1189\/jlb.1206763","article-title":"Crosstalk among Jak-STAT, Toll-like receptor, and ITAM-dependent pathways in macrophage activation","volume":"82","author":"X Hu","year":"2007","journal-title":"J Leukoc Biol"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0003317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T12:58:49Z","timestamp":1690721929000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0003317"}},"subtitle":[],"editor":[{"given":"Joseph M.","family":"Vinetz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,12,4]]},"references-count":54,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2014,12,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0003317","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,4]]}}}