{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:41:32Z","timestamp":1780465292578,"version":"3.54.1"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T00:00:00Z","timestamp":1420675200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0003445","type":"journal-article","created":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T19:15:59Z","timestamp":1420744559000},"page":"e3445","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":61,"title":["Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients"],"prefix":"10.1371","volume":"9","author":[{"given":"Yiu-Wing","family":"Kam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwoon-Yong","family":"Pok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Er","family":"Eng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Kiang","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simrandeep","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy W. L.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee-Sin","family":"Leo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee-Ching","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa F. P.","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,1,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"e83014","DOI":"10.1371\/journal.pone.0083014","article-title":"Characterization of the complete genome of chikungunya in Zhejiang, China, using a modified virus discovery method based on cDNA-AFLP","volume":"8","author":"Y Sun","year":"2013","journal-title":"PLoS One"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"410","DOI":"10.4269\/ajtmh.12-0681","article-title":"Kinetics of Chikungunya Infections during an Outbreak in Southern Thailand, 2008-2009","volume":"90","author":"S Chusri","year":"2014","journal-title":"Am J Trop Med Hyg"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/1743-422X-10-169","article-title":"Comparative analysis of the genome sequences and replication profiles of chikungunya virus isolates within the East, Central and South African (ECSA) lineage","volume":"10","author":"KC Chen","year":"2013","journal-title":"Virol J"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e2137","DOI":"10.1371\/journal.pntd.0002137","article-title":"Chikungunya virus-associated long-term arthralgia: a 36-month prospective longitudinal study","volume":"7","author":"C Schilte","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S0140-6736(14)60185-9","article-title":"Chikungunya in the Americas","volume":"383","author":"I Leparc-Goffart","year":"2014","journal-title":"Lancet"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1086\/590003","article-title":"Persistent arthralgia associated with chikungunya virus: a study of 88 adult patients on reunion island","volume":"47","author":"G Borgherini","year":"2008","journal-title":"Clin Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.micinf.2009.09.003","article-title":"Immuno-biology of Chikungunya and implications for disease intervention","volume":"11","author":"YW Kam","year":"2009","journal-title":"Microbes Infect"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.antiviral.2013.09.013","article-title":"Ten years of dengue drug discovery: progress and prospects","volume":"100","author":"SP Lim","year":"2013","journal-title":"Antiviral Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/1469-0691.12211","article-title":"West Nile virus in Europe: emergence, epidemiology, diagnosis, treatment, and prevention","volume":"19","author":"V Sambri","year":"2013","journal-title":"Clin Microbiol Infect"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"4586","DOI":"10.1128\/JCM.01228-10","article-title":"Concurrent isolation of chikungunya virus and dengue virus from a patient with coinfection resulting from a trip to Singapore","volume":"48","author":"SF Chang","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref11","first-page":"749","article-title":"Coinfection with dengue and chikungunya\u2014a case report","volume":"110","author":"KJ Kumar","year":"2012","journal-title":"J Indian Med Assoc"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"720","DOI":"10.4269\/ajtmh.2012.11-0704","article-title":"A comparative study of clinical features between monotypic and dual infection cases with Chikungunya virus and dengue virus in West Bengal, India","volume":"86","author":"D Taraphdar","year":"2012","journal-title":"Am J Trop Med Hyg"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"20730","DOI":"10.2807\/1560-7917.ES2014.19.10.20730","article-title":"Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola, January 2014","volume":"19","author":"R Parreira","year":"2014","journal-title":"Euro Surveill"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e1786","DOI":"10.1371\/journal.pntd.0001786","article-title":"Simple clinical and laboratory predictors of Chikungunya versus dengue infections in adults","volume":"6","author":"VJ Lee","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jviromet.2004.11.002","article-title":"Development of a TaqMan RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses","volume":"124","author":"B Pastorino","year":"2005","journal-title":"J Virol Methods"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.jcv.2007.05.008","article-title":"Molecular diagnosis and analysis of Chikungunya virus","volume":"39","author":"CJ Edwards","year":"2007","journal-title":"J Clin Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jcv.2007.04.015","article-title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus","volume":"39","author":"SR Santhosh","year":"2007","journal-title":"J Clin Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.patbio.2007.07.002","article-title":"Molecular and serological diagnosis of Chikungunya virus infection","volume":"55","author":"P Grivard","year":"2007","journal-title":"Pathol Biol (Paris)"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jviromet.2008.01.004","article-title":"Evaluation of the first commercial chikungunya virus indirect immunofluorescence test","volume":"149","author":"N Litzba","year":"2008","journal-title":"J Virol Methods"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1002\/emmm.201200213","article-title":"Early neutralizing IgG response to Chikungunya virus in infected patients targets a dominant linear epitope on the E2 glycoprotein","volume":"4","author":"YW Kam","year":"2012","journal-title":"EMBO Mol Med"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"13005","DOI":"10.1128\/JVI.01780-12","article-title":"Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development","volume":"86","author":"YW Kam","year":"2012","journal-title":"J Virol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6295","DOI":"10.4049\/jimmunol.1300304","article-title":"An essential role of antibodies in the control of Chikungunya virus infection","volume":"190","author":"FM Lum","year":"2013","journal-title":"J Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e95647","DOI":"10.1371\/journal.pone.0095647","article-title":"Unique epitopes recognized by antibodies induced in chikungunya virus-infected non-human primates: implications for the study of immunopathology and vaccine development","volume":"9","author":"YW Kam","year":"2014","journal-title":"PLoS One"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"664","DOI":"10.4269\/ajtmh.1975.24.664","article-title":"The distribution and prevalence of group A arbovirus neutralizing antibodies among human populations in Southeast Asia and the Pacific islands","volume":"24","author":"RB Tesh","year":"1975","journal-title":"Am J Trop Med Hyg"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e1168","DOI":"10.1371\/journal.pone.0001168","article-title":"Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus","volume":"2","author":"M Vazeille","year":"2007","journal-title":"PLoS One"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.4269\/ajtmh.2010.10-0279","article-title":"Seroprevalence survey of Chikungunya virus in Bagan Panchor, Malaysia","volume":"83","author":"SM Ayu","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e753","DOI":"10.1371\/journal.pntd.0000753","article-title":"Evaluation of Chikungunya diagnostic assays: differences in sensitivity of serology assays in two independent outbreaks","volume":"4","author":"G Yap","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.3201\/eid1812.120471","article-title":"Reemergence of Chikungunya virus in Cambodia","volume":"18","author":"V Duong","year":"2012","journal-title":"Emerg Infect Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1093\/trstmh\/trt083","article-title":"Risk factors for symptomatic and asymptomatic chikungunya infection","volume":"107","author":"P Nakkhara","year":"2013","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e2483","DOI":"10.1371\/journal.pntd.0002483","article-title":"Evidence for endemic chikungunya virus infections in Bandung, Indonesia","volume":"7","author":"H Kosasih","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1002\/jmv.23875","article-title":"Correlation of plasma viral loads and presence of Chikungunya IgM antibodies with cytokine\/chemokine levels during acute Chikungunya virus infection","volume":"86","author":"V Reddy","year":"2014","journal-title":"J Med Virol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e787","DOI":"10.1371\/journal.pntd.0000787","article-title":"Arboviral etiologies of acute febrile illnesses in Western South America, 2000\u20132007","volume":"4","author":"BM Forshey","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nrrheum.2012.64","article-title":"Arthritogenic alphaviruses\u2014an overview","volume":"8","author":"A Suhrbier","year":"2012","journal-title":"Nat Rev Rheumatol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/jmv.10085","article-title":"Combined detection and genotyping of Chikungunya virus by a specific reverse transcription-polymerase chain reaction","volume":"67","author":"F Hasebe","year":"2002","journal-title":"J Med Virol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1128\/JCM.22.2.250-254.1985","article-title":"Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization","volume":"22","author":"DM Morens","year":"1985","journal-title":"J Clin Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"285","DOI":"10.4049\/jimmunol.99.2.285","article-title":"A plaque reduction test for dengue virus neutralizing antibodies","volume":"99","author":"PK Russell","year":"1967","journal-title":"J Immunol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0092-8674(01)00291-4","article-title":"Virus evolution: how does an enveloped virus make a regular structure?","volume":"105","author":"JH Strauss","year":"2001","journal-title":"Cell"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1128\/CVI.00140-08","article-title":"Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection","volume":"15","author":"K Bessoff","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"333","DOI":"10.4269\/ajtmh.2008.78.333","article-title":"Seroprevalence of Chikungunya virus (CHIKV) infection on Lamu Island, Kenya, October 2004","volume":"78","author":"K Sergon","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e111305","DOI":"10.1371\/journal.pone.0111305","article-title":"Cytokines in acute chikungunya","volume":"9","author":"A Venugopalan","year":"2014","journal-title":"PLoS One"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1093\/infdis\/jit811","article-title":"Serum antibody response to matrix protein 2 following natural infection with 2009 pandemic influenza A(H1N1) virus in humans","volume":"209","author":"W Zhong","year":"2014","journal-title":"J Infect Dis"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1111\/j.1537-2995.2012.03960.x","article-title":"Viremic profiles in asymptomatic and symptomatic chikungunya fever: a blood transfusion threat?","volume":"53","author":"H Appassakij","year":"2013","journal-title":"Transfusion"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1126\/science.286.5447.2156","article-title":"Control of early viral and bacterial distribution and disease by natural antibodies","volume":"286","author":"AF Ochsenbein","year":"1999","journal-title":"Science"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/S0167-5699(00)01754-0","article-title":"Natural antibodies and complement link innate and acquired immunity","volume":"21","author":"AF Ochsenbein","year":"2000","journal-title":"Immunol Today"},{"key":"ref45","first-page":"2897","article-title":"Protection by natural IgG: a sweet partnership with soluble lectins does the trick! EMBO J","volume":"32","author":"I Puga","year":"2013"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"10118","DOI":"10.1128\/JVI.75.21.10118-10131.2001","article-title":"Evolutionary relationships and systematics of the alphaviruses","volume":"75","author":"AM Powers","year":"2001","journal-title":"J Virol"},{"key":"ref47","unstructured":"NovaTec Immunodiagnostica Gmbh. ELISA for CHKV antigen-specific IgG. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.novatec-id.com\/products\/infectious-diseases\/virology\/chikungunya-new\" xlink:type=\"simple\">http:\/\/www.novatec-id.com\/products\/infectious-diseases\/virology\/chikungunya-new<\/ext-link>. Accessed 19 May 2014."},{"key":"ref48","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1586\/eci.13.20","article-title":"Immune monitoring of children with respiratory syncytial virus infection","volume":"9","author":"A Mejias","year":"2013","journal-title":"Expert Rev Clin Immunol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1086\/432478","article-title":"Humoral immune response in acute hepatitis C virus infection","volume":"41","author":"DM Netski","year":"2005","journal-title":"Clin Infect Dis"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1093\/infdis\/jis033","article-title":"Early appearance of neutralizing immunoglobulin G3 antibodies is associated with chikungunya virus clearance and long-term clinical protection","volume":"205","author":"YW Kam","year":"2012","journal-title":"J Infect Dis"}],"container-title":["PLoS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0003445","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T17:40:29Z","timestamp":1690825229000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0003445"}},"subtitle":[],"editor":[{"given":"Ann M.","family":"Powers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,1,8]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,1,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0003445","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,8]]}}}