{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:22:02Z","timestamp":1791354122487,"version":"4.3.1"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T00:00:00Z","timestamp":1431043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0003711","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T18:08:21Z","timestamp":1431108501000},"page":"e0003711","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Morphological and Molecular Descriptors of the Developmental Cycle of Babesia divergens Parasites in Human Erythrocytes"],"prefix":"10.1371","volume":"9","author":[{"given":"Ingrid","family":"Rossouw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Maritz-Olivier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jandeli","family":"Niemand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riette","family":"van Biljon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annel","family":"Smit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Olivier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyn-Marie","family":"Birkholtz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,5,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1128\/CMR.13.3.451-469.2000","article-title":"Babesiosis","volume":"13","author":"MJ Homer","year":"2000","journal-title":"Clin Micro Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1007\/s15010-013-0526-8","article-title":"Human Babesiosis in Europe: what clinicians need to know","volume":"41","author":"A Hildebrandt","year":"2013","journal-title":"Infection"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Gray J, Dautel H, Estrada-Pe\u00f1a A, Kahl O, Lindgren E (2009) Effects of climate change on ticks and tick-borne diseases in Europe. Interdisc Perspec Infect Dis 2009.","DOI":"10.1155\/2009\/593232"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.vetpar.2006.01.048","article-title":"Chemotherapy against babesiosis","volume":"138","author":"HJ Vial","year":"2006","journal-title":"Vet Parasitol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbiopara.2008.11.013","article-title":"An overview of the <italic>Babesia<\/italic>, <italic>Plasmodium<\/italic> and <italic>Theileria<\/italic> genomes: A comparative perspective","volume":"164","author":"AO Lau","year":"2009","journal-title":"Mol Biochem Parasitol"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Vannier E, Krause PJ (2009) Update on babesiosis. Interdisc Perspect Infect Diseas 2009.","DOI":"10.1155\/2009\/984568"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.2174\/092986712799828355","article-title":"Current advances in detection and treatment of babesiosis","volume":"19","author":"J Mosqueda","year":"2012","journal-title":"Current Med Chem"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1086\/649859","article-title":"Emergence of resistance to azithromycin-atovaquone in immunocompromised patients with <italic>Babesia microti<\/italic> infection","volume":"50","author":"GP Wormser","year":"2010","journal-title":"Clin Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1056\/NEJM200011163432004","article-title":"Atovaquone and azithromycin for the treatment of babesiosis","volume":"343","author":"PJ Krause","year":"2000","journal-title":"New Eng J of Med"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.ijpara.2006.02.022","article-title":"Characterization and gene expression of <italic>Babesia bovis<\/italic> elongation factor-1alpha","volume":"36","author":"CE Suarez","year":"2006","journal-title":"Int J Parasitol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e148","DOI":"10.1371\/journal.ppat.0030148","article-title":"Genome sequence of <italic>Babesia bovis<\/italic> and comparative analysis of apicomplexan hemoprotozoa","volume":"3","author":"KA Brayton","year":"2007","journal-title":"PLoS Pathog"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/BF00930815","article-title":"Sexual cycle of <italic>Babesia divergens<\/italic> confirmed by DNA measurements","volume":"76","author":"U Mackenstedt","year":"1990","journal-title":"Parasitol Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.ijpara.2008.03.001","article-title":"Babesiosis: recent insights into an ancient disease","volume":"38","author":"K-P Hunfeld","year":"2008","journal-title":"Int J Parasitol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0248-4900(91)90010-K","article-title":"Lipid trafficking between high density lipoproteins and <italic>Babesia divergens<\/italic>-infected human erythrocytes","volume":"73","author":"A Valentin","year":"1991","journal-title":"Biol Cell"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1051\/vetres\/2009020","article-title":"<italic>Babesia<\/italic> and its hosts: adaptation to long-lasting interactions as a way to achieve efficient transmission","volume":"40","author":"A Chauvin","year":"2009","journal-title":"Vet Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ttbdis.2009.11.003","article-title":"Zoonotic babesiosis: overview of the disease and novel aspects of pathogen identity","volume":"1","author":"J Gray","year":"2010","journal-title":"Ticks Tick-borne Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.exppara.2008.10.008","article-title":"<italic>Plasmodium falciparum<\/italic>: Development and validation of a measure of intraerythrocytic growth using SYBR Green I in a flow cytometer","volume":"121","author":"S Izumiyama","year":"2009","journal-title":"Exp Parasitol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1093\/bioinformatics\/btp129","article-title":"dCAS: a desktop application for cDNA sequence annotation","volume":"25","author":"Y Guo","year":"2009","journal-title":"Bioinformatics"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2307\/3280287","article-title":"Synchronization of <italic>Plasmodium falciparum<\/italic> erythrocytic stages in culture","author":"C Lambros","year":"1979","journal-title":"J Parasitol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ijpara.2010.07.012","article-title":"The progression of the intra-erythrocytic cell cycle of <italic>Plasmodium falciparum<\/italic> and the role of the centriolar plaques in asynchronous mitotic division during schizogony","volume":"41","author":"DE Arnot","year":"2011","journal-title":"Int J Parasitol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1297-9716-42-62","article-title":"The invasion process of bovine erythrocyte by <italic>Babesia divergen<\/italic>s: knowledge from an in vitro assay","volume":"42","author":"Y Sun","year":"2011","journal-title":"Vet Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.exppara.2012.10.007","article-title":"Validation of BdCCp2 as a marker for Babesia divergens sexual stages in ticks","volume":"133","author":"CA Becker","year":"2013","journal-title":"Exp Parasitol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/BF00219605","article-title":"An electron microscopic study of <italic>Babesia microti<\/italic> invading erythrocytes","volume":"169","author":"MA Rudzinska","year":"1976","journal-title":"Cell Tissue Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.molbiopara.2010.06.011","article-title":"Identification of three CCp genes in Babesia divergens: novel markers for sexual stages parasites","volume":"174","author":"CA Becker","year":"2010","journal-title":"Mol Biochem Parasitol"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01194-14","article-title":"High-Quality Draft Genome Sequence of <italic>Babesia divergens<\/italic>, the Etiological Agent of Cattle and Human Babesiosis","volume":"2","author":"I Cuesta","year":"2014","journal-title":"Genome Announc"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"7113","DOI":"10.1093\/nar\/gku322","article-title":"The evolutionary dynamics of variant antigen genes in Babesia reveal a history of genomic innovation underlying host-parasite interaction","volume":"42","author":"AP Jackson","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1371\/journal.pbio.0000005","article-title":"The transcriptome of the intraerythrocytic developmental cycle of <italic>Plasmodium falciparum<\/italic>","volume":"1","author":"Z Bozdech","year":"2003","journal-title":"PLoS Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1128\/AAC.00022-14","article-title":"Evaluation of a fluorescence-based method for antibabesial drug screening","volume":"58","author":"A Guswanto","year":"2014","journal-title":"Antimicro Agents Chemother"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1186\/1475-2875-12-187","article-title":"Designing the next generation of medicines for malaria control and eradication","volume":"12","author":"JN Burrows","year":"2013","journal-title":"Malar J"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.pt.2014.07.004","article-title":"Defining the biology component of the drug discovery strategy for malaria eradication","volume":"30","author":"D Leroy","year":"2014","journal-title":"Trends Parasitol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1128\/AAC.42.4.818","article-title":"In vitro evaluation of drug susceptibilities of <italic>Babesia divergens<\/italic> Isolates","volume":"42","author":"P Brasseur","year":"1998","journal-title":"Antimicro Agents Chemother"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1002\/cyto.a.20541","article-title":"Monitoring <italic>Plasmodium falciparum<\/italic> growth and development by UV flow cytometry using an optimized Hoechst\u2010thiazole orange staining strategy","volume":"73","author":"BT Grimberg","year":"2008","journal-title":"Cytometry Part A"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/BF00934377","article-title":"Cytological and immunological responses to <italic>Babesia divergens<\/italic> in different hosts: Ox, gerbil, man","volume":"77","author":"A Gorenflot","year":"1991","journal-title":"Parasitol Res"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"W Trager","year":"1976","journal-title":"Science"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"334","DOI":"10.4269\/ajtmh.2003.68.334","article-title":"Growth inhibitory effect of triclosan on equine and bovine <italic>Babesia<\/italic> parasites","volume":"68","author":"S Bork","year":"2003","journal-title":"Am J Trop Med Hyg"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"6624","DOI":"10.1021\/jm200463z","article-title":"Discovery of novel alkylated (bis) urea and (bis) thiourea polyamine analogues with potent antimalarial activities","volume":"54","author":"BK Verlinden","year":"2011","journal-title":"J Med Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"4635","DOI":"10.1074\/jbc.M807085200","article-title":"Co-inhibition of <italic>Plasmodium falciparum<\/italic> S-adenosylmethionine decarboxylase\/ornithine decarboxylase reveals perturbation-specific compensatory mechanisms by transcriptome, proteome, and metabolome analyses","volume":"284","author":"AC van Brummelen","year":"2009","journal-title":"J Biol Chem"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"14863","DOI":"10.1073\/pnas.95.25.14863","article-title":"Cluster analysis and display of genome-wide expression patterns","volume":"95","author":"MB Eisen","year":"1998","journal-title":"Proc Nat Acad Sci"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.1093\/bioinformatics\/bti610","article-title":"Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research","volume":"21","author":"A Conesa","year":"2005","journal-title":"Bioinformatics"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0003711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T08:08:40Z","timestamp":1589011720000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0003711"}},"subtitle":[],"editor":[{"given":"Pamela L. C.","family":"Small","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,8]]},"references-count":39,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0003711","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,8]]}}}