{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T05:53:00Z","timestamp":1780725180026,"version":"3.54.1"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2015,7,10]],"date-time":"2015-07-10T00:00:00Z","timestamp":1436486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0003884","type":"journal-article","created":{"date-parts":[[2015,7,10]],"date-time":"2015-07-10T14:08:41Z","timestamp":1436537321000},"page":"e0003884","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":61,"title":["Development of Recombinase Polymerase Amplification Assays for Detection of Orientia tsutsugamushi or Rickettsia typhi"],"prefix":"10.1371","volume":"9","author":[{"given":"Chien-Chung","family":"Chao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Belinskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Mei","family":"Ching","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,7,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S39","DOI":"10.1086\/518145","article-title":"Rickettsiae and Rickettsial Infections: The Current State of Knowledge","volume":"45","author":"DH Walker","year":"2007","journal-title":"Clin Infec Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.7205\/MILMED.170.4S.49","article-title":"History of U. S. Military Contributions to the study of Rickettsial Diseases","volume":"170","author":"MF Bavaro","year":"2005","journal-title":"Military Medicine"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"S145","DOI":"10.1086\/339908","article-title":"The past and present threat of rickettsial diseases to military medicine and international public health","volume":"34","author":"DJ Kelly","year":"2002","journal-title":"Clinical Infect. Dis."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1097\/00001432-200410000-00008","article-title":"Rickettsial infections\u2014a threat to travellers?","volume":"17","author":"JM Rolain","year":"2004","journal-title":"Curr Opin Infect Dis"},{"key":"ref5","first-page":"239","article-title":"Rickettsial diseases and their serological diagnosis","volume":"46","author":"E Kovacova","year":"2000","journal-title":"Clin Lab"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1128\/JCM.35.11.2715-2727.1997","article-title":"Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases","volume":"35","author":"B La Scola","year":"1997","journal-title":"J Clin Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1574-695X.2011.00899.x","article-title":"Widespread use of real-time PCR for rickettsial diagnosis","volume":"64","author":"A Renvois\u00e9","year":"2012","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1111\/j.1574-695X.2011.00910.x","article-title":"Development of a duplex real-time PCR for the detection of Rickettsia spp. and typhus group rickettsia in clinical samples","volume":"64","author":"S Giulieri","year":"2012","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/j.1469-0691.2008.02242.x","article-title":"Assessment of a quantitative multiplex 5\u2019 nuclease real-time PCR for spotted fever and typhus group rickettsioses and <italic>Orientia tsutsugamushi<\/italic>","volume":"2","author":"JAJ Prakash","year":"2009","journal-title":"Clin Microbiol Infect Suppl"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"351","DOI":"10.4269\/ajtmh.2004.70.351","article-title":"Development of a quantitative real-time polymerase chain reaction assay specific for <italic>Orientia tsutsugamushi<\/italic>","volume":"70","author":"J Jiang","year":"2004","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.1469-0691.2008.02671.x","article-title":"A highly sensitive quantitative real- time PCR assay based on the <italic>groEL<\/italic> gene of contemporary 339 Thai strains of <italic>Orientia tsutsugamushi<\/italic>","volume":"15","author":"DH Paris","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1099\/00207713-47-2-252","article-title":"Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the <italic>rickettsiae<\/italic>","volume":"47","author":"V Roux","year":"1997","journal-title":"Int J Syst Bacteriol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.4269\/ajtmh.2005.73.1083","article-title":"A highly sensitive and specific real-time PCR assay for the detection of spotted fever and typhus group <italic>Rickettsiae<\/italic>","volume":"73","author":"J Stenos","year":"2005","journal-title":"Am J Trop Med Hyg"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jcv.2007.02.001","article-title":"Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method","volume":"39","author":"M Ihira","year":"2007","journal-title":"J Clin Vir"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1128\/JCM.41.6.2616-2622.2003","article-title":"Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium and M. intercellulare in sputum samples","volume":"41","author":"T Iwamoto","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jcv.2006.07.011","article-title":"Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method","volume":"37","author":"S Iwata","year":"2006","journal-title":"J Clin Vir"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2665","DOI":"10.1128\/JCM.00216-08","article-title":"Discriminating between varicella-zoster virus vaccine and wild-type strains by loop-mediated isothermal amplification","volume":"46","author":"Y Higashimoto","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/j.1574-695X.2008.00417.x","article-title":"Rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method","volume":"53","author":"J Miyagawa","year":"2008","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"3653","DOI":"10.1128\/JCM.01188-08","article-title":"Real-time reverse-transcription loop-mediated isothermal amplification for rapid detection of Rift Valley Fever virus","volume":"46","author":"CN Peyrefitte","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.trstmh.2008.04.040","article-title":"Simple, rapid and sensitive detection of <italic>Orientia tsutusgamushi<\/italic> by loop-isothermal DNA amplification","volume":"102","author":"DH Paris","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref21","first-page":"112","article-title":"Loop-mediated isothermal amplification assay targeting the 47-kDa gene of <italic>Orientia tsutsugamushi<\/italic>: a rapid and sensitive alternative to real-time PCR","volume":"1","author":"E Huber","year":"2012","journal-title":"J Med Microbiol and Diag"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA Detection Using recombination Proteins","volume":"4","author":"O Piepenburg","year":"2004","journal-title":"PLoS Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jmoldx.2013.08.003","article-title":"Sensitive and Rapid Detection of Chlamydia trachomatis by Recombinase Polymerase Amplification Directly from Urine Samples","volume":"16","author":"K Kr\u00f5lov","year":"2014","journal-title":"J Mol Diag"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e00135","DOI":"10.1128\/mBio.00135-13","article-title":"Rapid Detection of HIV-1 Proviral DNA for Early Infant Diagnosis Using Recombinase Polymerase Amplification","volume":"4","author":"DS Boyle","year":"2013","journal-title":"MBio"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e2244","DOI":"10.1371\/journal.pntd.0002244","article-title":"International External Quality Assessment of Molecular Detection of Rift Valley Fever Virus","volume":"7","author":"C Escadafal","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1128\/JCM.02704-12","article-title":"Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents","volume":"51","author":"M Euler","year":"2013","journal-title":"J Clin Microbiol"},{"key":"ref27","article-title":"Reverse Transcription Recombinase Polymerase Amplification Assay for the Detection of Middle East Respiratory Syndrome Coronavirus","volume":"5","author":"A Abd El Wahed","year":"2013","journal-title":"PLoS Curr"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e71642","DOI":"10.1371\/journal.pone.0071642","article-title":"A Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus","volume":"8","author":"A Abd El Wahed","year":"2013","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jviromet.2013.06.027","article-title":"A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay","volume":"193","author":"HM Amer","year":"2013","journal-title":"J Viro Meth"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1021\/ac403750z","article-title":"Nucleic Acid Test to Diagnose Cryptosporidiosis: Lab Assessment in Animal and Patient Specimens","volume":"86","author":"ZA Crannell","year":"2014","journal-title":"Anal Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1007\/s00604-014-1198-5","article-title":"Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens","volume":"181","author":"S Kersting","year":"2014","journal-title":"Microchim Acta"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1128\/JCM.06504-11","article-title":"Recombinase Polymerase Amplification Assay for Rapid Detection of <italic>Francisella tularensis<\/italic>","volume":"50","author":"M Euler","year":"2012","journal-title":"J Clin Microbio"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1373\/clinchem.2013.213504","article-title":"Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal\/Anal Samples","volume":"60","author":"RK Daher","year":"2014","journal-title":"Clin Chem"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.aca.2013.12.017","article-title":"Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis","volume":"811","author":"S Santiago-Felipe","year":"2014","journal-title":"Anal Chim Acta"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1002\/jmv.21490","article-title":"Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1","volume":"81","author":"KA Curtis","year":"2009","journal-title":"J Med Virol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1128\/JB.186.2.556-565.2004","article-title":"Comparative genomics of <italic>Rickettsia prowazekii<\/italic> Madrid E and Breinl strains","volume":"186","author":"H Ge","year":"2004","journal-title":"J Bacteriol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.mcp.2006.06.002","article-title":"Development of quantitative real-time PCR assay to detect <italic>Rickettsia typhi<\/italic> and <italic>Rickettsia felis<\/italic>, the causative agents of murine typhus and flea-borne spotted fever","volume":"21","author":"KM Henry","year":"2007","journal-title":"Mol Cell Probes"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"e54570","DOI":"10.1371\/journal.pone.0054570","article-title":"An Intradermal Inoculation Model of Scrub Typhus in Swiss CD-1 Mice Demonstrates More Rapid Dissemination of Virulent Strains of Orientia tsutsugamushi","volume":"8","author":"P Sunyakumthorn","year":"2013","journal-title":"PLoS ONE"},{"key":"ref39","unstructured":"Jackson A, Belinskya T, Ching W-M, Chao C-C (2013) Recombinase Polymerase Amplification Assay for Rapid Detection of Q fever. Abstract # 719, 62<sup>nd<\/sup> Annual ASTMH meeting, Nov 13\u201317, 2013, Marriott Wardman Park, Washington DC, USA"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1128\/IAI.01298-08","article-title":"Identification of cross-reactive epitopes on the conserved 47-kilodalton antigen of <italic>Orientia tsutsugamushi<\/italic> and human serine protease","volume":"77","author":"HW Chen","year":"2009","journal-title":"Infect Immun"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1089\/vbz.2012.1112","article-title":"Diversity of the 47-kD HtrA Nucleic Acid and Translated Amino Acid Sequences from 17 Recent Human Isolates of Orientia","volume":"13","author":"J Jiang","year":"2013","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"4404","DOI":"10.1128\/JCM.01526-10","article-title":"Isolation of a novel Orientia species (O. Chuto sp. Nov.) from a patient infected in Dubai","volume":"48","author":"L Izzard","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/JCM.01927-08","article-title":"Association of high <italic>Orientia tsutsugamushi<\/italic> DNA loads with disease of greater severity in adults with scrub typhus","volume":"47","author":"P Sonthayanon","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1128\/JCM.02786-13","article-title":"Loop-Mediated Isothermal Amplification for Rickettsia typhi (the Causal Agent of Murine Typhus): Problems with Diagnosis at the Limit of Detection","volume":"52","author":"S Dittrich","year":"2014","journal-title":"J. Clin. Microbiol"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0003884","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T18:11:12Z","timestamp":1599243072000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0003884"}},"subtitle":[],"editor":[{"given":"Didier","family":"Raoult","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,7,10]]},"references-count":44,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2015,7,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0003884","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725638667.793508296","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,10]]}}}