{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:57:48Z","timestamp":1791262668351,"version":"4.1.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T00:00:00Z","timestamp":1450396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0004299","type":"journal-article","created":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T13:46:05Z","timestamp":1450446365000},"page":"e0004299","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":44,"title":["Genomic and Proteomic Studies on the Mode of Action of Oxaboroles against the African Trypanosome"],"prefix":"10.1371","volume":"9","author":[{"given":"Deuan C.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo J.","family":"Foth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Urbaniak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Patterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han B.","family":"Ong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Berriman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan H.","family":"Fairlamb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,12,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0140-6736(09)60829-1","article-title":"Human African trypanosomiasis","volume":"375","author":"R Brun","year":"2010","journal-title":"Lancet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"e1691","DOI":"10.1371\/journal.pntd.0001691","article-title":"Untreated human infections by Trypanosoma brucei gambiense are not 100% fatal","volume":"6","author":"V Jamonneau","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref3","first-page":"257","article-title":"Epidemiology of human African trypanosomiasis","volume":"6","author":"JR Franco","year":"2014","journal-title":"Clin Epidemiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e1859","DOI":"10.1371\/journal.pntd.0001859","article-title":"Estimating and mapping the population at risk of sleeping sickness","volume":"6","author":"PP Simarro","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1017\/S0031182013002102","article-title":"The journey towards elimination of gambiense human African trypanosomiasis: not far, nor easy","volume":"141","author":"JR Franco","year":"2014","journal-title":"Parasitology"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1098\/rsif.2011.0654","article-title":"Predicting the effect of climate change on African trypanosomiasis: integrating epidemiology with parasite and vector biology","volume":"9","author":"S Moore","year":"2012","journal-title":"J R Soc Interface"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.cub.2013.11.044","article-title":"Meiosis and haploid gametes in the pathogen <italic>Trypanosoma brucei<\/italic>","volume":"24","author":"L Peacock","year":"2014","journal-title":"Curr Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"677","DOI":"10.2217\/fmb.11.44","article-title":"Development of novel drugs for human African trypanosomiasis","volume":"6","author":"R Brun","year":"2011","journal-title":"Future Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.drup.2007.02.004","article-title":"Drugs and drug resistance in African trypanosomiasis","volume":"10","author":"V Delespaux","year":"2007","journal-title":"Drug Resist Updat"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/S0140-6736(09)61117-X","article-title":"Nifurtimox-eflornithine combination therapy for second-stage African <italic>Trypanosoma brucei gambiense<\/italic> trypanosomiasis: a multicentre, randomised, phase III, non-inferiority trial","volume":"374","author":"G Priotto","year":"2009","journal-title":"Lancet"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1128\/AAC.00332-10","article-title":"Cross-resistance to nitro drugs and implications for treatment of human African trypanosomiasis","volume":"54","author":"AY Sokolova","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e1001204","DOI":"10.1371\/journal.ppat.1001204","article-title":"A molecular mechanism for eflornithine resistance in African trypanosomes","volume":"6","author":"IM Vincent","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182013001224","article-title":"Emerging paradigms in anti-infective drug design","volume":"141","author":"MP Barrett","year":"2014","journal-title":"Parasitology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.coph.2012.05.001","article-title":"Antiparasitic agents: new drugs on the horizon","volume":"12","author":"P Maser","year":"2012","journal-title":"Curr Opin Pharmacol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"4379","DOI":"10.1128\/AAC.00498-10","article-title":"Discovery of novel orally bioavailable oxaborole 6-carboxamides that demonstrate cure in a murine model of late-stage central nervous system African trypanosomiasis","volume":"54","author":"B Nare","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e1151","DOI":"10.1371\/journal.pntd.0001151","article-title":"SCYX-7158, an orally-active benzoxaborole for the treatment of stage 2 Human African trypanosomiasis","volume":"5","author":"RT Jacobs","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1038\/srep02475","article-title":"Discovery of a potent benzoxaborole-based anti-pneumococcal agent targeting leucyl-tRNA synthetase","volume":"3","author":"QH Hu","year":"2013","journal-title":"Sci Rep"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1126\/science.1142189","article-title":"An antifungal agent inhibits an aminoacyl-tRNA synthetase by trapping tRNA in the editing site","volume":"316","author":"FL Rock","year":"2007","journal-title":"Science"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4462","DOI":"10.1016\/j.bmc.2014.04.065","article-title":"The unique chemistry of benzoxaboroles: current and emerging applications in biotechnology and therapeutic treatments","volume":"22","author":"CT Liu","year":"2014","journal-title":"Bioorg Med Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1002\/anie.201208749","article-title":"Target identification for small bioactive molecules: finding the needle in the haystack","volume":"52","author":"S Ziegler","year":"2013","journal-title":"Angew Chem Int Ed Engl"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev-pharmtox-011613-135915","article-title":"Antiparasitic chemotherapy: from genomes to mechanisms","volume":"54","author":"D Horn","year":"2014","journal-title":"Annu Rev Pharmacol Toxicol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e506","DOI":"10.1371\/journal.pntd.0000506","article-title":"Adenosine kinase of T. b. rhodesiense identified as the putative target of 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine using chemical proteomics","volume":"3","author":"S Kuettel","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0166-6851(99)00002-X","article-title":"A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in <italic>Trypanosoma brucei<\/italic>","volume":"99","author":"E Wirtz","year":"1999","journal-title":"Mol Biochem Parasitol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1742-4658.2008.06788.x","article-title":"A comparative study of methylglyoxal metabolism in trypanosomatids","volume":"276","author":"N Greig","year":"2009","journal-title":"FEBS J"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1016\/j.bcp.2010.07.038","article-title":"Identification of a kappa-opioid agonist as a potent and selective lead for drug development against human African trypanosomiasis","volume":"80","author":"DC Jones","year":"2010","journal-title":"Biochem Pharmacol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0001-706X(97)00079-X","article-title":"The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.b.rhodesiense and T.b.gambiense) in vitro","volume":"68","author":"B Raz","year":"1997","journal-title":"Acta Trop"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"985","DOI":"10.2307\/3282883","article-title":"Continuous cultivation of <italic>Trypanosoma brucei<\/italic> blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers","volume":"75","author":"H Hirumi","year":"1989","journal-title":"J Parasitol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1021\/pr400086y","article-title":"Global quantitative SILAC phosphoproteomics reveals differential phosphorylation is widespread between the procyclic and bloodstream form lifecycle stages of <italic>Trypanosoma brucei<\/italic>","volume":"12","author":"MD Urbaniak","year":"2013","journal-title":"J Proteome Res"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1021\/ml100013s","article-title":"Discovery of novel benzoxaborole-based potent antitrypanosomal agents","volume":"1","author":"D Ding","year":"2010","journal-title":"ACS Med Chem Letters"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"J Cox","year":"2008","journal-title":"Nat Biotechnol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1021\/pr101065j","article-title":"Andromeda: a peptide search engine integrated into the MaxQuant environment","volume":"10","author":"J Cox","year":"2011","journal-title":"J Proteome Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkp851","article-title":"TriTrypDB: a functional genomic resource for the Trypanosomatidae","volume":"38","author":"M Aslett","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1002\/(SICI)1097-0320(20000201)39:2<141::AID-CYTO7>3.0.CO;2-O","article-title":"Apoptosis can be detected in attached colonic adenocarcinoma HT29 cells using annexin V binding, but not by TUNEL assay or sub-G0 DNA content","volume":"39","author":"RG Clarke","year":"2000","journal-title":"Cytometry"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1186\/1471-2164-13-341","article-title":"A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers","volume":"13","author":"MA Quail","year":"2012","journal-title":"BMC Genomics"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1126\/science.1112642","article-title":"The genome of the African trypanosome <italic>Trypanosoma brucei<\/italic>","volume":"309","author":"M Berriman","year":"2005","journal-title":"Science"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"H Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/nbt.2839","article-title":"ProteomeXchange provides globally coordinated proteomics data submission and dissemination","volume":"32","author":"JA Vizcaino","year":"2014","journal-title":"Nat Biotechnol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1017\/S003118201300098X","article-title":"Pharmacokinetics and pharmacodynamics utilizing unbound target tissue exposure as part of a disposition-based rationale for lead optimization of benzoxaboroles in the treatment of Stage 2 Human African Trypanosomiasis","volume":"141","author":"S Wring","year":"2014","journal-title":"Parasitology"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1101\/gr.115089.110","article-title":"High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome","volume":"21","author":"S Alsford","year":"2011","journal-title":"Genome Res"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbiopara.2007.01.017","article-title":"Cell cycle regulation in <italic>Trypanosoma brucei<\/italic>","volume":"153","author":"TC Hammarton","year":"2007","journal-title":"Mol Biochem Parasitol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.mib.2007.10.005","article-title":"Cytokinesis in trypanosomatids","volume":"10","author":"TC Hammarton","year":"2007","journal-title":"Curr Opin Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1186\/s12864-015-1505-5","article-title":"RNA interference screen reveals a high proportion of mitochondrial proteins essential for correct cell cycle progress in <italic>Trypanosoma brucei<\/italic>","volume":"16","author":"DE Mbang-Benet","year":"2015","journal-title":"BMC Genomics"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1128\/AAC.02578-12","article-title":"Induced resistance to methionyl-tRNA synthetase inhibitors in <italic>Trypanosoma brucei<\/italic> is due to overexpression of the target","volume":"57","author":"RM Ranade","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1146\/annurev-biochem-093010-153308","article-title":"Ubiquitin-Like Proteins","volume":"81","author":"AG van der Veen","year":"2012","journal-title":"Annu Rev Biochem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1186\/1747-1028-3-5","article-title":"Emerging roles of the SUMO pathway in mitosis","volume":"3","author":"M Dasso","year":"2008","journal-title":"Cell Division"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.yexcr.2009.12.017","article-title":"The small ubiquitin-like modifier (SUMO) is essential in cell cycle regulation in Trypanosoma brucei","volume":"316","author":"SH Liao","year":"2010","journal-title":"Exp Cell Res"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/prot.22409","article-title":"Solution structure of SUMO from Trypanosoma brucei and its interaction with Ubc9","volume":"76","author":"Q Shang","year":"2009","journal-title":"Proteins-Structure Function and Bioinformatics"},{"key":"ref48","article-title":"Different proteomic strategies to identify genuine Small Ubiquitin-like MOdifier targets and their modification sites in <italic>Trypanosoma brucei<\/italic> procyclic forms","author":"PA Iribarren","year":"2015","journal-title":"Cell Microbiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"e180","DOI":"10.7717\/peerj.180","article-title":"SUMOylation in Trypanosoma brucei","volume":"1","author":"CA Klein","year":"2013","journal-title":"PeerJ"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.molbiopara.2004.04.006","article-title":"RNA interference of <italic>Trypanosoma brucei<\/italic> topoisomerase IB: both subunits are essential","volume":"136","author":"RP Bakshi","year":"2004","journal-title":"Mol Biochem Parasitol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/mmi.12853","article-title":"Homoserine and quorum-sensing acyl homoserine lactones as alternative sources of threonine: a potential role for homoserine kinase in insect-stage <italic>Trypanosoma brucei<\/italic>","volume":"95","author":"HB Ong","year":"2015","journal-title":"Mol Microbiol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1101\/gad.1214604","article-title":"SUMO and ubiquitin in the nucleus: different functions, similar mechanisms?","volume":"18","author":"G Gill","year":"2004","journal-title":"Genes Dev"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0004299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:35:37Z","timestamp":1588970137000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0004299"}},"subtitle":[],"editor":[{"given":"Michael P.","family":"Pollastri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,12,18]]},"references-count":52,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2015,12,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0004299","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,18]]}}}