{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:36:17Z","timestamp":1791272177322,"version":"4.1.0"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T00:00:00Z","timestamp":1453939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0004312","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T18:48:53Z","timestamp":1454006933000},"page":"e0004312","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction"],"prefix":"10.1371","volume":"10","author":[{"given":"Johanna L.","family":"H\u00f6\u00f6g","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Lacomble","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cedric","family":"Bouchet-Marquis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Briggs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Hoenger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Gull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,1,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/cne.20513","article-title":"Development and properties of stereociliary link types in hair cells of the mouse cochlea","volume":"485","author":"RJ Goodyear","year":"2005","journal-title":"J Comp Neurol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.mib.2007.09.014","article-title":"The cell biology of Trypanosoma brucei differentiation","volume":"10","author":"K Fenn","year":"2007","journal-title":"Current Opinion in Microbiology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/jcs.74.1.1","article-title":"Differentiation in Trypanosoma brucei: host-parasite cell junctions and their persistence during acquisition of the variable antigen coat","volume":"74","author":"L Tetley","year":"1985","journal-title":"J Cell Sci"},{"issue":"Pt 1","key":"ref4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1017\/S0031182097001042","article-title":"Cytoskeletal architecture and components involved in the attachment of Trypanosoma congolense epimastigotes","volume":"115","author":"P Beattie","year":"1997","journal-title":"Parasitology"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"3117","DOI":"10.1096\/fj.09-151381","article-title":"Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans","volume":"24","author":"E Gluenz","year":"2010","journal-title":"FASEB J"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/j.1550-7408.1973.tb00909.x","article-title":"The mode of attachment of Trypanosoma vivax in the proboscis of the tsetse fly Glossina fuscipes: an ultrastructural study of the epimastigote stage of the trypanosome","volume":"20","author":"K Vickerman","year":"1973","journal-title":"J Protozool"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1242\/jcs.045740","article-title":"Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography","volume":"122","author":"S Lacomble","year":"2009","journal-title":"J Cell Sci"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S1369-5274(03)00092-4","article-title":"Host\u2013parasite interactions and trypanosome morphogenesis: a flagellar pocketful of goodies","volume":"6","author":"K Gull","year":"2003","journal-title":"Current Opinion in Microbiology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1038\/35300","article-title":"Paraflagellar rod is vital for trypanosome motility","volume":"391","author":"P Bastin","year":"1998","journal-title":"Nature"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijpara.2009.10.005","article-title":"The paraflagellar rod of kinetoplastid parasites: From structure to components and function","volume":"40","author":"N Portman","year":"2010","journal-title":"International Journal for Parasitology. Australian Society for Parasitology Inc"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0035-9203(62)90072-X","article-title":"The mechanism of cyclical development in trypanosomes of the Trypanosoma brucei sub-group: an hypothesis based on ultrastructural observations","volume":"56","author":"K Vickerman","year":"1962","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jsb.2012.01.009","article-title":"Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei","volume":"178","author":"JL H\u00f6\u00f6g","year":"2012","journal-title":"J Struct Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1242\/jcs.166447","article-title":"A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes","volume":"128","author":"JD Sunter","year":"2015","journal-title":"J Cell Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1126\/science.1063775","article-title":"A Trypanosome Structure Involved in Transmitting Cytoplasmic Information During Cell Division","volume":"294","author":"FF Moreira-Leite","year":"2001","journal-title":"Science"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e01479","DOI":"10.7554\/eLife.01479","article-title":"Modes of flagellar assembly in Chlamydomonas reinhardtii and Trypanosoma brucei","volume":"3","author":"JL H\u00f6\u00f6g","year":"2014","journal-title":"eLife"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"5748","DOI":"10.1242\/jcs.139139","article-title":"A cell-body groove housing the new flagellum tip suggests an adaptation of cellular morphogenesis for parasitism in the bloodstream form of Trypanosoma brucei","volume":"126","author":"L Hughes","year":"2013","journal-title":"J Cell Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1242\/jcs.00995","article-title":"The flagella connector of Trypanosoma brucei: an unusual mobile transmembrane junction","volume":"117","author":"LJ Briggs","year":"2004","journal-title":"J Cell Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1242\/jcs.03203","article-title":"Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation","volume":"119","author":"J Davidge","year":"2006","journal-title":"J Cell Sci"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1098\/rstb.1989.0037","article-title":"The Cell Division Cycle of Trypanosoma brucei brucei: Timing of Event Markers and Cytoskeletal Modulations","volume":"323","author":"T Sherwin","year":"1989","journal-title":"Philosophical Transactions of the Royal Society B: Biological Sciences"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1038\/sj.emboj.7600366","article-title":"Cryo-electron microscopy of vitreous sections","volume":"23","author":"A Al-Amoudi","year":"2004","journal-title":"EMBO J"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1242\/jcs.074161","article-title":"Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei","volume":"123","author":"S Lacomble","year":"2010","journal-title":"J Cell Sci"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jsb.2005.01.003","article-title":"Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy","volume":"150","author":"A Alamoudi","year":"2005","journal-title":"J Struct Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1242\/jcs.02969","article-title":"Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes","volume":"119","author":"C Gadelha","year":"2006","journal-title":"J Cell Sci"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/0092-8674(89)90691-0","article-title":"Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration","volume":"56","author":"N Hirokawa","year":"1989","journal-title":"Cell"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/338355a0","article-title":"Identification of globular mechanochemical heads of kinesin","volume":"338","author":"JM Scholey","year":"1989","journal-title":"Nature"},{"key":"ref26","first-page":"23","article-title":"Intraflagellar transport (IFT) role in ciliary assembly, resorption and signalling","volume":"85","author":"LB Pedersen","year":"2008","journal-title":"Curr Top Dev Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1083\/jcb.131.6.1517","article-title":"The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane","volume":"131","author":"KG Kozminski","year":"1995","journal-title":"J Cell Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1083\/jcb.200905103","article-title":"Electron-tomographic analysis of intraflagellar transport particle trains in situ","volume":"187","author":"G Pigino","year":"2009","journal-title":"J Cell Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0091-679X(10)96008-1","article-title":"Ultrastructural investigation methods for Trypanosoma brucei","volume":"96","author":"JL H\u00f6\u00f6g","year":"2010","journal-title":"Methods Cell Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1083\/jcb.17.1.208","article-title":"The use of lead citrate at high pH as an electron-opaque stain in electron microscopy","volume":"17","author":"ES Reynolds","year":"1963","journal-title":"J Cell Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"116","author":"JR Kremer","year":"1996","journal-title":"J Struct Biol"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0004312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T17:42:03Z","timestamp":1654191723000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0004312"}},"subtitle":[],"editor":[{"given":"Armando","family":"Jardim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,1,28]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,1,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0004312","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,28]]}}}