{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:46:53Z","timestamp":1782409613874,"version":"3.54.5"},"reference-count":18,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T00:00:00Z","timestamp":1459814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0004593","type":"journal-article","created":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T19:51:55Z","timestamp":1459885915000},"page":"e0004593","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["The \u201cBuruli Score\u201d: Development of a Multivariable Prediction Model for Diagnosis of Mycobacterium ulcerans Infection in Individuals with Ulcerative Skin Lesions, Akonolinga, Cameroon"],"prefix":"10.1371","volume":"10","author":[{"given":"Yolanda K.","family":"Mueller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Bastard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Nkemenang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Comte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Ehounou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Eyangoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Rusch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Earnest Njih","family":"Tabah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence Toutous","family":"Trellu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Francois","family":"Etard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,4,5]]},"reference":[{"issue":"2","key":"ref1","first-page":"191","article-title":"Diagnosis of Mycobacterium ulcerans infection (Buruli ulcer) at a treatment centre in Ghana: a retrospective analysis of laboratory results of clinically diagnosed cases","volume":"13","author":"E Mensah-Quainoo","year":"2008","journal-title":"TropMedIntHealth"},{"key":"ref2","article-title":"Laboratory diagnosis of buruli ulcer: a manual for health care providers","author":"F Portaels","year":"2014"},{"issue":"8","key":"ref3","first-page":"1055","article-title":"Comparative study of the sensitivity of different diagnostic methods for the laboratory diagnosis of Buruli ulcer disease","volume":"48","author":"KH Herbinger","year":"2009","journal-title":"ClinInfectDis"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"e0003477","DOI":"10.1371\/journal.pntd.0003477","article-title":"Identification of the Mycobacterium ulcerans protein MUL_3720 as a promising target for the development of a diagnostic test for Buruli ulcer","volume":"9","author":"A Dreyer","year":"2015","journal-title":"PLoS neglected tropical diseases"},{"issue":"9","key":"ref5","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1136\/heartjnl-2011-301246","article-title":"Risk prediction models: I. Development, internal validation, and assessing the incremental value of a new (bio)marker","volume":"98","author":"KG Moons","year":"2012","journal-title":"Heart"},{"issue":"5","key":"ref6","first-page":"900","article-title":"Combining PCR with microscopy to reduce costs of laboratory diagnosis of Buruli ulcer","volume":"85","author":"D Yeboah-Manu","year":"2011","journal-title":"AmJTropMedHyg"},{"issue":"6","key":"ref7","first-page":"e466","article-title":"Prevalence of Buruli ulcer in Akonolinga health district, Cameroon: results of a cross sectional survey","volume":"3","author":"K Porten","year":"2009","journal-title":"PLoSNeglTropDis"},{"issue":"9","key":"ref8","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.1128\/JCM.41.9.4231-4237.2003","article-title":"Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA","volume":"41","author":"S Rondini","year":"2003","journal-title":"Journal of clinical microbiology"},{"key":"ref9","article-title":"Latent Class Analysis","author":"A McCutcheon","year":"1987"},{"issue":"2","key":"ref10","first-page":"e11","article-title":"Evaluation of latent class analysis and decision thresholds to guide the diagnosis of pediatric tuberculosis in a Rwandan reference hospital","volume":"29","author":"L Tuyisenge","year":"2010","journal-title":"PediatrInfectDisJ"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"e4385","DOI":"10.1371\/journal.pntd.0004385","article-title":"Differential diagnosis of skin ulcers in a Mycobacterium ulcerans endemic area: data from a prospective study in Cameroon","volume":"10","author":"L Toutous Trellu","year":"2016","journal-title":"PLoS neglected tropical diseases"},{"issue":"2","key":"ref12","first-page":"167","article-title":"Buruli ulcer in Ghana: results of a national case search","volume":"8","author":"G Amofah","year":"2002","journal-title":"EmergInfectDis"},{"issue":"7","key":"ref13","doi-asserted-by":"crossref","first-page":"e422","DOI":"10.1016\/S2214-109X(14)70223-2","article-title":"Clinical epidemiology of laboratory-confirmed Buruli ulcer in Benin: a cohort study","volume":"2","author":"QB Vincent","year":"2014","journal-title":"Lancet GlobHealth"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"341","DOI":"10.5694\/mja12.10087","article-title":"Epidemiology, clinical features and diagnosis of Mycobacterium ulcerans in an Australian population","volume":"196","author":"SC Boyd","year":"2012","journal-title":"The Medical journal of Australia"},{"issue":"3","key":"ref15","first-page":"291","article-title":"Buruli ulcer","volume":"27","author":"F Portaels","year":"2009","journal-title":"ClinDermatol"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1128\/IAI.01588-07","article-title":"Mycolactone is responsible for the painlessness of Mycobacterium ulcerans infection (buruli ulcer) in a murine study","volume":"76","author":"J En","year":"2008","journal-title":"Infection and immunity"},{"issue":"7","key":"ref17","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1016\/j.cell.2014.04.040","article-title":"Mycobacterial toxin induces analgesia in buruli ulcer by targeting the angiotensin pathways","volume":"157","author":"E Marion","year":"2014","journal-title":"Cell"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"e445","DOI":"10.1371\/journal.pntd.0000445","article-title":"A word of caution against the stigma trend in neglected tropical disease research and control","volume":"3","author":"J Muela Ribera","year":"2009","journal-title":"PLoS neglected tropical diseases"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0004593","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T13:28:19Z","timestamp":1540301299000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0004593"}},"subtitle":[],"editor":[{"given":"Christian","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,4,5]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,4,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0004593","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,5]]}}}