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Codeco","year":"2009","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"issue":"12","key":"ref6","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.3201\/eid2112.151167","article-title":"Outbreak of Exanthematous Illness Associated with Zika, Chikungunya, and Dengue Viruses, Salvador, Brazil","volume":"21","author":"CW Cardoso","year":"2015","journal-title":"Emerging infectious diseases"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/s12916-015-0348-x","article-title":"Emergence and potential for spread of Chikungunya virus in Brazil","volume":"13","author":"MR Nunes","year":"2015","journal-title":"BMC Med"},{"key":"ref8","unstructured":"PAHO\/WHO 1 December 2015. Epidemiological Alert Neurological syndrome, congenital malformations, and Zika virus infection. Implications for public health in the Americas. Available at: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.paho.org\/hq\/index.php?option=com_docman&task=doc_download&Itemid=&gid=32405&lang=en\" xlink:type=\"simple\">http:\/\/www.paho.org\/hq\/index.php?option=com_docman&amp;task=doc_download&amp;Itemid=&amp;gid=32405&amp;lang=en<\/ext-link>. Accessed 16.12.2015."},{"issue":"9","key":"ref9","article-title":"Zika virus infection complicated by Guillain-Barre syndrome\u2014case report, French Polynesia, December 2013","volume":"19","author":"E Oehler","year":"2014","journal-title":"Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1473-3099(07)70107-X","article-title":"Chikungunya, an epidemic arbovirosis","volume":"7","author":"G Pialoux","year":"2007","journal-title":"The Lancet infectious diseases"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0035-9203(64)90201-9","article-title":"Zika Virus Infection in Man","volume":"58","author":"DI Simpson","year":"1964","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"10","key":"ref12","doi-asserted-by":"crossref","first-page":"O595","DOI":"10.1111\/1469-0691.12707","article-title":"Rapid spread of emerging Zika virus in the Pacific area","volume":"20","author":"D Musso","year":"2014","journal-title":"Clin Microbiol Infect"},{"issue":"7","key":"ref13","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.medmal.2014.04.008","article-title":"Current Zika virus epidemiology and recent epidemics","volume":"44","author":"S Ioos","year":"2014","journal-title":"Medecine et maladies infectieuses"},{"issue":"24","key":"ref14","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika virus outbreak on Yap Island, Federated States of Micronesia","volume":"360","author":"MR Duffy","year":"2009","journal-title":"N Engl J Med"},{"issue":"10","key":"ref15","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.3201\/eid2110.150847","article-title":"Zika Virus Outbreak, Bahia, Brazil","volume":"21","author":"GS Campos","year":"2015","journal-title":"Emerging infectious diseases"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jcv.2015.11.014","article-title":"First detection of autochthonous Zika virus transmission in a HIV-infected patient in Rio de Janeiro, Brazil","volume":"74","author":"GA Calvet","year":"2016","journal-title":"Journal of Clinical Virology"},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1590\/0074-02760150192","article-title":"First report of autochthonous transmission of Zika virus in Brazil","volume":"110","author":"C Zanluca","year":"2015","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"issue":"6","key":"ref18","doi-asserted-by":"crossref","first-page":"679","DOI":"10.20344\/amp.7341","article-title":"Zika Virus: A New Chapter in the History of Medicine","volume":"28","author":"C Brito","year":"2015","journal-title":"Acta Medica Portugesa"},{"key":"ref19","unstructured":"Besnard M, Mallet HP: Increase of cerebral congenital malformations among newborns and fetus in French Polynesia, 2014\u20132015, following a Zika virus outbreak. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/m.box.com\/shared_item\/https%3A%2F%2Fapp.box.com%2Fs%2Fptvqdargwr10m3ag849rv59c5iditco7\" xlink:type=\"simple\">https:\/\/m.box.com\/shared_item\/https%3A%2F%2Fapp.box.com%2Fs%2Fptvqdargwr10m3ag849rv59c5iditco7<\/ext-link>."},{"key":"ref20","article-title":"Ocular Findings in Infants With Microcephaly Associated With Presumed Zika Virus Congenital Infection in Salvador, Brazil","author":"B de Paula Freitas","year":"2016","journal-title":"JAMA Ophthalmol"},{"key":"ref21","article-title":"Zika Virus Infection in Pregnant Women in Rio de Janeiro\u2014Preliminary Report","author":"P Brasil","year":"2016","journal-title":"N Engl J Med"},{"key":"ref22","unstructured":"Brazil Ministry of Health press release. Available at: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/portalsaude.saude.gov.br\/index.php\/cidadao\/principal\/agencia-saude\/17701-confirmacao-do-zika-virus-no-brasil\" xlink:type=\"simple\">http:\/\/portalsaude.saude.gov.br\/index.php\/cidadao\/principal\/agencia-saude\/17701-confirmacao-do-zika-virus-no-brasil<\/ext-link>. Accessed 19.01.2016."},{"issue":"8","key":"ref23","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007","volume":"14","author":"RS Lanciotti","year":"2008","journal-title":"Emerg Infect Dis"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/JCM.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction","volume":"30","author":"RS Lanciotti","year":"1992","journal-title":"Journal of clinical microbiology"},{"key":"ref25","article-title":"R: A language and environment for statistical computing","year":"2015"},{"key":"ref26","unstructured":"Instituto Nacional de Meteorologia (INMET). Available at: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.inmet.gov.br\/sonabra\/pg_iframe.php?codEst=A652&mesAno=2015\" xlink:type=\"simple\">http:\/\/www.inmet.gov.br\/sonabra\/pg_iframe.php?codEst=A652&amp;mesAno=2015<\/ext-link>. Accessed 16.12.2015."},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1590\/S0074-02761986000200019","article-title":"An outbreak of dengue virus at Rio de Janeiro\u20141986","volume":"81","author":"HG Schatzmayr","year":"1986","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3201\/eid2102.141363","article-title":"Potential sexual transmission of Zika virus","volume":"21","author":"D Musso","year":"2015","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"ref29","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3201\/eid1705.101939","article-title":"Probable non-vector-borne transmission of Zika virus, Colorado, USA","volume":"17","author":"BD Foy","year":"2011","journal-title":"Emerging infectious diseases"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3201\/eid2101.140894","article-title":"Detection of Zika virus in urine","volume":"21","author":"AC Gourinat","year":"2015","journal-title":"Emerging infectious diseases"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jcv.2015.04.021","article-title":"Detection of Zika virus in saliva","volume":"68","author":"D Musso","year":"2015","journal-title":"J Clin Virol"},{"key":"ref32","unstructured":"Brazil. Ministry of Health. Microcephaly. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/portalsaude.saude.gov.br\/index.php\/cidadao\/principal\/agencia-saude\/21254-ministerio-da-saude-atualiza-numeros-de-microcefalia-relacionados-ao-zika\" xlink:type=\"simple\">http:\/\/portalsaude.saude.gov.br\/index.php\/cidadao\/principal\/agencia-saude\/21254-ministerio-da-saude-atualiza-numeros-de-microcefalia-relacionados-ao-zika<\/ext-link>. Accessed 14.01.2016."},{"issue":"10","key":"ref33","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.3201\/eid2110.151125","article-title":"Zika Virus Transmission from French Polynesia to Brazil","volume":"21","author":"D Musso","year":"2015","journal-title":"Emerging infectious diseases"},{"issue":"10016","key":"ref34","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0140-6736(16)00080-5","article-title":"Anticipating the international spread of Zika virus from Brazil","volume":"387","author":"Bogoch II","year":"2016","journal-title":"Lancet"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0004636","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T13:58:18Z","timestamp":1600437498000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0004636"}},"subtitle":[],"editor":[{"given":"Ann M","family":"Powers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,4,12]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,4,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0004636","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,12]]}}}