{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:55:12Z","timestamp":1791352512723,"version":"4.3.1"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2016,9,29]],"date-time":"2016-09-29T00:00:00Z","timestamp":1475107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0004953","type":"journal-article","created":{"date-parts":[[2016,9,29]],"date-time":"2016-09-29T15:37:01Z","timestamp":1475163421000},"page":"e0004953","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":72,"title":["A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus"],"prefix":"10.1371","volume":"10","author":[{"given":"Pranav","family":"Patel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abd El Wahed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumar","family":"Faye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Pr\u00fcger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Kaiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasikanya","family":"Thaloengsok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukathida","family":"Ubol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anavaj","family":"Sakuntabhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Leparc-Goffart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank T.","family":"Hufert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou A.","family":"Sall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Niedrig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,9,29]]},"reference":[{"issue":"1","key":"ref1","first-page":"28","article-title":"An epidemic of virus disease in Southern Province, Tanganyika Territory, in 1952\u201353. I. Clinical features","volume":"49","author":"MC Robinson","year":"1955","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4269\/ajtmh.1968.17.107","article-title":"Chikungunya disease","volume":"17","author":"JJ Deller Jr.","year":"1968","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"984","DOI":"10.4269\/ajtmh.1969.18.984","article-title":"Dengue chikungunya virus infection in man in Thailand, 1962\u20131964. 3. Clinical, epidemiologic, and virologic observations on disease in non-indigenous white persons","volume":"18","author":"SB Halstead","year":"1969","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1097\/MD\/0b013e31806010a5","article-title":"Chikungunya infection: an emerging rheumatism among travelers returned from Indian Ocean islands. Report of 47 cases","volume":"86","author":"F Simon","year":"2007","journal-title":"Medicine (Baltimore)"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"616","DOI":"10.4269\/ajtmh.2005.72.616","article-title":"Differential infectivities of o'nyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes","volume":"72","author":"DL Vanlandingham","year":"2005","journal-title":"The American journal of tropical medicine and hygiene"},{"key":"ref6","first-page":"409","article-title":"Outbreak and spread of chikungunya","volume":"82","author":"WH Organization","year":"2007","journal-title":"Wkly Epidemiol Rec"},{"issue":"Pt 9","key":"ref7","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1099\/vir.0.82858-0","article-title":"Changing patterns of chikungunya virus: re-emergence of a zoonotic arbovirus","volume":"88","author":"AM Powers","year":"2007","journal-title":"The Journal of general virology"},{"issue":"9602","key":"ref8","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1016\/S0140-6736(07)61779-6","article-title":"Infection with chikungunya virus in Italy: an outbreak in a temperate region","volume":"370","author":"G Rezza","year":"2007","journal-title":"Lancet"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"333","DOI":"10.4269\/ajtmh.2008.78.333","article-title":"Seroprevalence of Chikungunya virus (CHIKV) infection on Lamu Island, Kenya, October 2004","volume":"78","author":"K Sergon","year":"2008","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.3201\/eid1210.060529","article-title":"Chikungunya outbreaks caused by African genotype, India","volume":"12","author":"PN Yergolkar","year":"2006","journal-title":"Emerging infectious diseases"},{"issue":"13","key":"ref11","doi-asserted-by":"crossref","first-page":"6497","DOI":"10.1128\/JVI.01603-09","article-title":"Genome-scale phylogenetic analyses of chikungunya virus reveal independent emergences of recent epidemics and various evolutionary rates","volume":"84","author":"SM Volk","year":"2010","journal-title":"Journal of virology"},{"issue":"11","key":"ref12","doi-asserted-by":"crossref","first-page":"e1168","DOI":"10.1371\/journal.pone.0001168","article-title":"Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus","volume":"2","author":"M Vazeille","year":"2007","journal-title":"PLoS ONE"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"12279","DOI":"10.1038\/srep12279","article-title":"Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Philippines 2012","volume":"5","author":"KK Tan","year":"2015","journal-title":"Scientific reports"},{"key":"ref14","article-title":"Zika virus in Brazil and the danger of infestation by Aedes (Stegomyia) mosquitoes","author":"CB Marcondes","year":"2015","journal-title":"Rev Soc Bras Med Trop"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4103\/0019-5359.104781","article-title":"Laboratory diagnosis of chikungunya virus: do we really need it?","volume":"65","author":"M Dash","year":"2011","journal-title":"Indian J Med Sci"},{"issue":"8","key":"ref16","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1373\/clinchem.2007.086595","article-title":"Development of a sensitive real-time reverse transcriptase PCR assay with an internal control to detect and quantify chikungunya virus","volume":"53","author":"P Laurent","year":"2007","journal-title":"Clin Chem"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"521","DOI":"10.4269\/ajtmh.2007.77.521","article-title":"Rapid detection and quantification of Chikungunya virus by a one-step reverse transcription polymerase chain reaction real-time assay","volume":"77","author":"F Carletti","year":"2007","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/jmv.10085","article-title":"Combined detection and genotyping of Chikungunya virus by a specific reverse transcription-polymerase chain reaction","volume":"67","author":"F Hasebe","year":"2002","journal-title":"Journal of medical virology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1743-422X-7-13","article-title":"Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection","volume":"7","author":"PS Ho","year":"2010","journal-title":"Virology journal"},{"issue":"9","key":"ref20","doi-asserted-by":"crossref","first-page":"3014","DOI":"10.1128\/JCM.01024-09","article-title":"Performance of the RealStar Chikungunya virus real-time reverse transcription-PCR kit","volume":"47","author":"M Panning","year":"2009","journal-title":"J Clin Microbiol"},{"issue":"1\u20132","key":"ref21","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jviromet.2004.11.002","article-title":"Development of a TaqMan RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses","volume":"124","author":"B Pastorino","year":"2005","journal-title":"J Virol Methods"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1046\/j.1439-0450.2002.00535.x","article-title":"Specific detection of chikungunya virus using a RT-PCR\/nested PCR combination","volume":"49","author":"M Pfeffer","year":"2002","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jcv.2007.04.015","article-title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus","volume":"39","author":"SR Santhosh","year":"2007","journal-title":"J Clin Virol"},{"issue":"7","key":"ref24","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"O Piepenburg","year":"2006","journal-title":"PLoS Biol"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"e71642","DOI":"10.1371\/journal.pone.0071642","article-title":"A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus","volume":"8","author":"A Abd El Wahed","year":"2013","journal-title":"PLoS ONE"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"e0129682","DOI":"10.1371\/journal.pone.0129682","article-title":"Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection","volume":"10","author":"A Abd El Wahed","year":"2015","journal-title":"PLoS ONE"},{"key":"ref27","article-title":"Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus","volume":"5","author":"A Abd El Wahed","year":"2013","journal-title":"PLoS currents"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jcv.2015.05.004","article-title":"Diagnostics-in-a-Suitcase: Development of a portable and rapid assay for the detection of the emerging avian influenza A (H7N9) virus","volume":"69","author":"A Abd El Wahed","year":"2015","journal-title":"J Clin Virol"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jviromet.2013.06.027","article-title":"A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay","volume":"193","author":"HM Amer","year":"2013","journal-title":"J Virol Methods"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00135-13","article-title":"Rapid Detection of HIV-1 Proviral DNA for Early Infant Diagnosis Using Recombinase Polymerase Amplification","volume":"4","author":"DS Boyle","year":"2013","journal-title":"mBio"},{"issue":"8","key":"ref31","doi-asserted-by":"crossref","first-page":"e103091","DOI":"10.1371\/journal.pone.0103091","article-title":"Rapid detection of Mycobacterium tuberculosis by recombinase polymerase amplification","volume":"9","author":"DS Boyle","year":"2014","journal-title":"PLoS ONE"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"e2730","DOI":"10.1371\/journal.pntd.0002730","article-title":"Rapid molecular assays for the detection of yellow fever virus in low-resource settings","volume":"8","author":"C Escadafal","year":"2014","journal-title":"PLoS negl trop dis"},{"issue":"44","key":"ref33","article-title":"Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015","volume":"20","author":"O Faye","year":"2015","journal-title":"Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin"},{"issue":"12","key":"ref34","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"M Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref35","author":"M Kaiser","year":"2014"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"635","DOI":"10.4269\/ajtmh.13-0627","article-title":"Vector competence of Aedes aegypti and Aedes vittatus (Diptera: Culicidae) from Senegal and Cape Verde archipelago for West African lineages of chikungunya virus","volume":"91","author":"CT Diagne","year":"2014","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"5","key":"ref37","doi-asserted-by":"crossref","first-page":"764","DOI":"10.3201\/eid1305.070015","article-title":"Chikungunya virus in US travelers returning from India, 2006","volume":"13","author":"RS Lanciotti","year":"2007","journal-title":"Emerging infectious diseases"},{"issue":"3","key":"ref38","doi-asserted-by":"crossref","first-page":"416","DOI":"10.3201\/eid1403.070906","article-title":"Chikungunya fever in travelers returning to Europe from the Indian Ocean region, 2006","volume":"14","author":"M Panning","year":"2008","journal-title":"Emerging infectious diseases"},{"key":"ref39","article-title":"External quality assessment studies for laboratory performance of molecular and serological diagnosis of Chikungunya virus infection","author":"S Jacobsen","year":"2016","journal-title":"Journal of Clinical Virology"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"947","DOI":"10.4269\/ajtmh.2012.11-0721","article-title":"Rapid identification of Chikungunya and Dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method","volume":"87","author":"X Lu","year":"2012","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"2","key":"ref41","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1128\/JCM.01734-06","article-title":"Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay","volume":"45","author":"MM Parida","year":"2007","journal-title":"J Clin Microbiol"},{"issue":"12","key":"ref42","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"T Notomi","year":"2000","journal-title":"Nucleic acids research"},{"issue":"2","key":"ref43","doi-asserted-by":"crossref","first-page":"e0004472","DOI":"10.1371\/journal.pntd.0004472","article-title":"Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea","volume":"10","author":"Y Kurosaki","year":"2016","journal-title":"PLoS negl trop dis"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0004953","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T23:43:09Z","timestamp":1568418189000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0004953"}},"subtitle":[],"editor":[{"given":"Maya","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,9,29]]},"references-count":43,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2016,9,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0004953","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,29]]}}}