{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:56:59Z","timestamp":1787021819660,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pntd.0005790","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T00:00:00Z","timestamp":1501804800000}}],"reference-count":80,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T00:00:00Z","timestamp":1500508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0005790","type":"journal-article","created":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T13:38:50Z","timestamp":1500557930000},"page":"e0005790","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":54,"title":["Small subunit ribosomal metabarcoding reveals extraordinary trypanosomatid diversity in Brazilian bats"],"prefix":"10.1371","volume":"11","author":[{"given":"Maria Augusta","family":"Dario","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Moratelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Schwabl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin S.","family":"Llewellyn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,7,20]]},"reference":[{"key":"ref1","first-page":"33","article-title":"Chagas Disease in Latin America: an epidemiological update based on 2010 estimates","volume":"90","year":"2015","journal-title":"Wkly Epidemiol Rec"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1016\/j.ijpara.2004.08.011","article-title":"Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA","volume":"34","author":"PB Hamilton","year":"2004","journal-title":"Int J Parasitol"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1186\/1756-3305-6-221","article-title":"<italic>Trypanosoma livingstonei<\/italic>: a new species from African bats supports the bat seeding hypothesis for the <italic>Trypanosoma cruzi<\/italic> clade","volume":"6","author":"L Lima","year":"2013","journal-title":"Parasit Vectors"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.meegid.2008.10.011","article-title":"Phylogenetic analysis reveals the presence of the <italic>Trypanosoma cruzi<\/italic> clade in African terrestrial mammals","volume":"9","author":"PB Hamilton","year":"2009","journal-title":"Infect Genet Evol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0020-7519(98)00167-2","article-title":"A nested PCR for the ssrRNA gene detects <italic>Trypanosoma binneyi<\/italic> in platypus and <italic>Trypanosoma<\/italic> sp. in wombats and kangaroos in Australia","volume":"29","author":"HA Noyes","year":"1999","journal-title":"Int J Parasitol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2015.07.018","article-title":"The multiple and complex and changeable scenarios of the <italic>Trypanosoma cruzi<\/italic> transmission cycle in the sylvatic environment","volume":"151","author":"AM Jansen","year":"2015","journal-title":"Acta Trop"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.protis.2011.12.003","article-title":"Evolutionary insights from bat trypanosomes: morphological, developmental and phylogenetic evidence of a new species, <italic>Trypanosoma<\/italic> (<italic>Schizotrypanum<\/italic>) <italic>erneyi<\/italic> sp. nov. in African bats closely related to <italic>Trypanosoma<\/italic> (<italic>Schizotrypanum<\/italic>) <italic>cruzi<\/italic> and allied species","volume":"163","author":"L Lima","year":"2012","journal-title":"Protist"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1186\/s13071-015-1255-x","article-title":"New insights into the evolution of the <italic>Trypanosoma cruzi<\/italic> clade provided by a new trypanosome species tightly linked to Neotropical <italic>Pteronotus<\/italic> bats and related to an Australian lineage of trypanosomes","volume":"8","author":"L Lima","year":"2015","journal-title":"Parasit Vectors"},{"issue":"6118","key":"ref9","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1126\/science.1230835","article-title":"Comparative analysis of bat genomes provides insight into the evolution of flight and immunity","volume":"339","author":"G Zhang","year":"2013","journal-title":"Science"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"741","DOI":"10.3201\/eid2005.130539","article-title":"Bat flight and zoonotic viruses","volume":"20","author":"TJ O\u2019Shea","year":"2014","journal-title":"Emer Infect Dis"},{"key":"ref11","first-page":"843","article-title":"Sur une grande \u00e9pizootie de range","volume":"25","author":"A Carini","year":"1911","journal-title":"Ann Inst Pasteur (Paris)"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1099\/vir.0.020073-0","article-title":"Host immunity to repeated rabies virus infection in big brown bats","volume":"91","author":"AS Turmelle","year":"2010","journal-title":"J Gen Virol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.3201\/eid1612.100601","article-title":"<italic>Bartonella<\/italic> spp. in bats, Kenya","volume":"16","author":"M Kosoy","year":"2010","journal-title":"Emerg Infect Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.3201\/eid1508.090475","article-title":"Fatal borreliosos in bat caused by relapsing fever spirochete, United Kingdom","volume":"15","author":"NJ Evans","year":"2009","journal-title":"Emerg Infect Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"653","DOI":"10.4269\/ajtmh.1981.30.653","article-title":"Pathogenesis of experimental histoplasmosis in the bat, <italic>Artibeus lituratus<\/italic>","volume":"30","author":"DL Greer","year":"1981","journal-title":"Am J Trop Med Hyg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4161\/viru.22330","article-title":"Pathology in euthermic bats with white nose syndrome suggests a natural manifestation of immune reconstitution inflammatory syndrome","volume":"3","author":"CU Meteyer","year":"2012","journal-title":"Virulence"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.pt.2012.01.006","article-title":"The evolution of <italic>Trypanosoma cruzi<\/italic>: the \u201cbat seeding\u201d hypothesis","volume":"28","author":"PB Hamilton","year":"2012","journal-title":"Trends Parasitol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"17415","DOI":"10.1073\/pnas.1311016110","article-title":"High diversity of West African bat malaria parasites and a tight link with rodent <italic>Plasmodium<\/italic> taxa","volume":"110","author":"J Schaer","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"216","DOI":"10.2307\/3225804","article-title":"Filaroid nematodes in olfactory mucosa, olfactory bulb and brain ventricular ventricular system of bats","volume":"100","author":"JR Lichtenfels","year":"1981","journal-title":"Trans Am Microsc Soc"},{"key":"ref20","first-page":"247","article-title":"About Bats","author":"JE Ubelaker","year":"1970"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.tim.2014.12.004","article-title":"Bats as \u2018special\u2019 reservoirs for emerging zoonotic pathogens","volume":"23","author":"CE Brook","year":"2015","journal-title":"Trends Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/S0169-4758(99)01532-X","article-title":"The molecular evolution of trypanosomes","volume":"15","author":"JR Stevens","year":"1999","journal-title":"Parasitol Today"},{"key":"ref23","article-title":"The trypanosomes of mammals: a zoological monograph","author":"CA Hoare","year":"1972"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1002\/bies.200900181","article-title":"High-throughput DNA sequencing\u2014concepts and limitations","volume":"32","author":"M Kircher","year":"2010","journal-title":"BioEssays"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1111\/j.1755-0998.2011.03024.x","article-title":"Field guide to next-generation DNA sequencers","volume":"11","author":"TC Glenn","year":"2011","journal-title":"Mol Ecol Resour"},{"issue":"8","key":"ref26","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1111\/j.1365-294X.2011.05403.x","article-title":"Who is eating what: diet assessment using next generation sequencing","volume":"21","author":"F Pompanon","year":"2012","journal-title":"Mol Ecol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1111\/j.1365-294X.2012.05470.x","article-title":"Towards next-generation biodiversity assessment using DNA metabarcoding","volume":"21","author":"P Taberlet","year":"2012","journal-title":"Mol Ecol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature09442","article-title":"Origin of the human malaria parasite <italic>Plasmodium falciparum<\/italic> in gorillas","volume":"467","author":"W Liu","year":"2010","journal-title":"Nature"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1111\/ele.12162","article-title":"Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding","volume":"16","author":"Y Ji","year":"2013","journal-title":"Ecol Lett"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1007\/s00248-015-0684-6","article-title":"Amplicon-based pyrosequencing reveals high diversity of protistan parasites in ships\u2019 ballast water: implications for biogeography and infectious diseases","volume":"71","author":"KM Pagenkopp-Lohan","year":"2016","journal-title":"Microbial Ecol"},{"key":"ref31","first-page":"549","article-title":"Mammals of the Neotropics. The Central Neotropics: Ecuador, Peru, Bolivia, Brazil","author":"GAB Fonseca","year":"1999"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1186\/s13071-016-1754-4","article-title":"Ecological scenario and <italic>Trypanosoma cruzi<\/italic> DTU characterization of a fatal acute Chagas disease case transmitted orally (Esp\u00edrito Santo state, Brazil)","volume":"9","author":"MA Dario","year":"2016","journal-title":"Parasit Vectors"},{"key":"ref33","doi-asserted-by":"crossref","DOI":"10.7208\/chicago\/9780226282428.001.0001","article-title":"Mammals of South America: marsupials, xenarthrans, shrews, and bats","author":"AL Gardner","year":"2008"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1017\/S0031182008004824","article-title":"Trypanosomes in a declining species of threatened Australian marsupial, the brush-tailed bettong <italic>Bettongia penicillata<\/italic> (Marsupialia: Potoroidae)","volume":"135","author":"A Smith","year":"2008","journal-title":"Parasitology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1038\/ismej.2011.11","article-title":"Reproducibility and quantitation of amplicon sequencing-based detection","volume":"5","author":"J Zhou","year":"2011","journal-title":"ISME J"},{"key":"ref36","unstructured":"Joshi N, Fass J. Sickle: A sliding-window, adaptive, quality-based trimming tool for FastQ files (Version 1.29) [Software]. 2011."},{"key":"ref37","unstructured":"Andrews S. FastQC: a quality control tool for high throughput sequence data (Version 0.11.5, 2016). <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\" xlink:type=\"simple\">http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc<\/ext-link>."},{"issue":"D1","key":"ref38","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"C Quast","year":"2013","journal-title":"Nucl Acids Res"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nat Methods"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"RC Edgar","year":"2013","journal-title":"Nat Methods"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"JG Caporaso","year":"2010","journal-title":"Nat Methods"},{"issue":"12","key":"ref42","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0","volume":"30","author":"K Tamura","year":"2013","journal-title":"Mol Biol Evol"},{"issue":"9","key":"ref43","doi-asserted-by":"crossref","first-page":"e108603","DOI":"10.1371\/journal.pone.0108603","article-title":"High local diversity of <italic>Trypanosoma<\/italic> in a common bat species, and implications for the biogeography and taxonomy of the <italic>T<\/italic>. <italic>cruzi<\/italic> clade","volume":"9","author":"VM Cottontail","year":"2014","journal-title":"PLoS One"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.vetpar.2015.10.016","article-title":"High prevalence of <italic>Trypanosoma vegrandis<\/italic> in bats Western Australia","volume":"214","author":"JM Austen","year":"2015","journal-title":"Vet Parasitol"},{"issue":"10","key":"ref45","doi-asserted-by":"crossref","first-page":"e0139999","DOI":"10.1371\/journal.pone.0139999","article-title":"Trypanosomes, and Triatomines in Ecuador: New Insights into the Diversity, Transmission, and Origins of <italic>Trypanosoma cruzi<\/italic> and Chagas Disease","volume":"10","author":"CM Pinto","year":"2015","journal-title":"PLoS One"},{"issue":"11","key":"ref46","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1017\/S0031182016000925","article-title":"First finding of <italic>Trypanosoma cruzi<\/italic> II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina","volume":"143","author":"HD Argibay","year":"2016","journal-title":"Parasitology"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetpar.2016.05.002","article-title":"<italic>Trypanosoma teixeirae<\/italic>: a new species belonging to the <italic>T<\/italic>. <italic>cruzi<\/italic> clade causing trypanosomiasis in an Australian little red flying fox (<italic>Pteropus scapulatus<\/italic>)","volume":"223","author":"AD Barbosa","year":"2016","journal-title":"Vet Parasitol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.actatropica.2016.08.033","article-title":"Diversity of bats trypanosomes in hydroeletric area of Belo Monte in Brazilian Amazonia","volume":"164","author":"AP da Costa","year":"2016","journal-title":"Acta Trop"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.actatropica.2016.09.013","article-title":"Trypanosome species, including <italic>Trypanosoma cruzi<\/italic>, in sylvatic and peridomestic bats of Texas, USA","volume":"164","author":"CL Hodo","year":"2016","journal-title":"Acta Trop"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.actatropica.2015.12.004","article-title":"A comparative study of <italic>Trypanosoma cruzi<\/italic> infection in sylvatic mammals from a protected and a disturbed area in the Argentine Chaco","volume":"155","author":"MM Orozco","year":"2016","journal-title":"Acta Trop"},{"issue":"5","key":"ref51","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1590\/S0074-02762006000500010","article-title":"Dynamics of feeding and defecation in <italic>Triatoma vitticeps<\/italic> (Stal, 1859) (Hemiptera, Reduviidae, Triatominae) and its potential in the transmission of <italic>Trypanosoma cruzi<\/italic>","volume":"101","author":"CB dos Santos","year":"2006","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"7","key":"ref52","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.3201\/eid1907.121576","article-title":"Molecular epidemiologic source tracking of orally transmitted Chagas disease, Venezuela","volume":"19","author":"M Segovia","year":"2013","journal-title":"Emerg Infect Dis"},{"key":"ref53","first-page":"225","article-title":"Prevalencia de <italic>Trypanosoma cruzi<\/italic> en roedores y marsupiales en dos localidades de Manab\u00ed, Ecuador","volume":"71","author":"CM Pinto","year":"2003","journal-title":"Rev Pontif Univ Cat\u00f3lica Ecuad"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1645\/GE-886R.1","article-title":"Infection by trypanosomes in marsupials and rodents associated with human dwellings in Ecuador","volume":"92","author":"CM Pinto","year":"2006","journal-title":"J Parasitol"},{"issue":"6","key":"ref55","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1017\/S0031182009005861","article-title":"A new genotype of <italic>Trypanosoma cruzi<\/italic> associated with bats evidenced by phylogenetic analyses using SSU rDNA, cytochrome b and Histone H2B genes and genotyping based on ITS1 rDNA","volume":"136","author":"A Marcili","year":"2009","journal-title":"Parasitology"},{"issue":"6","key":"ref56","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1016\/j.meegid.2012.04.013","article-title":"TcBat a bat-exclusive lineage of <italic>Trypanosoma cruzi<\/italic> in the Panama Canal Zone, with comments on its classification and the use of the 18S rRNA gene for lineage identification","volume":"12","author":"CM Pinto","year":"2012","journal-title":"Infect Genet Evol"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.meegid.2013.06.022","article-title":"Trypanosome species in neo-tropical bats: biological, evolutionary and epidemiological implications","volume":"22","author":"JD Ram\u00edrez","year":"2014","journal-title":"Infect Genet Evol"},{"issue":"5","key":"ref58","doi-asserted-by":"crossref","first-page":"e0003765","DOI":"10.1371\/journal.pntd.0003765","article-title":"Multiplex real-time PCR assay using TaqMan probes for the identification of Trypanosoma cruzi DTUs in biological and clinical samples","volume":"9","author":"CI Cura","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"ref59","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1590\/S0074-02761991000100012","article-title":"<italic>Trypanosoma rangeli<\/italic> (Tejera, 1920) isolated from a sylvatic rodent (<italic>Echimys dasythrix<\/italic>) in Santa Catarina Island, Santa Catarina State: first report of this trypanosome in southern Brazil","volume":"86","author":"M Steindel","year":"1991","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"16","key":"ref60","doi-asserted-by":"crossref","first-page":"3361","DOI":"10.1111\/j.1365-294X.2007.03371.x","article-title":"Comparative phylogeography of <italic>Trypanosoma rangeli<\/italic> and <italic>Rhodnius<\/italic> (Hemiptera: Reduviidae) supports a long coexistence of parasite lineages and their sympatric vectors","volume":"16","author":"F Maia Da Silva","year":"2007","journal-title":"Mol Ecol"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.actatropica.2008.11.005","article-title":"<italic>Trypanosoma rangeli<\/italic> isolates of bats from Central Brazil: genotyping and phylogenetic analysis enable description of a new lineage using spliced-leader gene sequences","volume":"109","author":"F Maia da Silva","year":"2009","journal-title":"Acta Trop"},{"issue":"3","key":"ref62","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/j.1550-7408.1994.tb01506.x","article-title":"Randomly amplified polymorphic DNA (RAPD) and isoenzyme analysis of <italic>Trypanosoma rangeli<\/italic> strains","volume":"41","author":"M Steindel","year":"1994","journal-title":"J Eukaryot Microbiol"},{"issue":"12","key":"ref63","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1111\/j.1365-3156.2005.01511.x","article-title":"Field ecology of sylvatic <italic>Rhodnius<\/italic> populations (Heteroptera, Triatominae): risk factors for palm tree infestation in western Ecuador","volume":"10","author":"F Abad-Franch","year":"2005","journal-title":"Trop Med Int Health"},{"issue":"6","key":"ref64","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1093\/aob\/mcp197","article-title":"The evolution of bat pollination: a phylogenetic perspective","volume":"104","author":"TH Fleming","year":"2009","journal-title":"Ann Bot"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ympev.2007.03.023","article-title":"Patterns of coevolution between trypanosomes and their hosts deduced from ribosomal RNA and protein-coding gene phylogenies","volume":"44","author":"PB Hamilton","year":"2007","journal-title":"Mol Phylog Evol"},{"key":"ref66","first-page":"161","article-title":"Parasitic Protozoa","volume":"10","author":"RS Telford","year":"1995"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1017\/S0031182008004253","article-title":"Phylogeny of snake trypanosomes inferred by SSU rRNA sequences, their possible transmission by phlebotomines, and taxonomic appraisal by molecular, cross-infection and morphological analysis","volume":"135","author":"LB Viola","year":"2008","journal-title":"Parasitology"},{"issue":"6","key":"ref68","first-page":"776","article-title":"Molecular detection of <italic>Trypanosoma<\/italic> sp. and <italic>Blastocrithidia<\/italic> sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil","volume":"48","author":"ST Ferreira","year":"2015"},{"issue":"S1","key":"ref69","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1590\/S0074-02761999000700007","article-title":"Immunopathology of Chagas disease","volume":"94","author":"ZA Andrade","year":"1999","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"4","key":"ref70","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.pt.2014.01.008","article-title":"Chagas disease: the challenge of polyparasitism?","volume":"30","author":"CJ Perez","year":"2014","journal-title":"Trends Parasitol"},{"issue":"10","key":"ref71","doi-asserted-by":"crossref","first-page":"e846","DOI":"10.1371\/journal.pntd.0000846","article-title":"Coinfection with different <italic>Trypanosoma cruzi<\/italic> strains interferes with the host immune response to infection","volume":"4","author":"CM Rodrigues","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref72","first-page":"5","article-title":"Chagas disease in a wormy world","volume":"71","author":"M Gal\u00e1n-Puchades","year":"2012","journal-title":"Rev Ibero-Latinoam Parasitol"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1645\/12-156.1","article-title":"Isolation and phylogenetic relationships of bat trypanosomes from different biomes in Mato Grosso, Brazil","volume":"99","author":"A Marcili","year":"2013","journal-title":"J. Parasitol"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.ijpara.2009.08.015","article-title":"Phylogeographical, ecological and biological patterns shown by nuclear (ssrRNA and gGAPDH) and mitochondrial (Cyt b) genes of trypanosomes of the subgenus <italic>Schizotrypanum<\/italic> parasitic in Brazilian bats","volume":"40","author":"M Cavazzana Jr","year":"2010","journal-title":"Int J Parasitol"},{"issue":"8","key":"ref75","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1111\/j.1365-294X.2012.05550.x","article-title":"Bioinformatic challenges for DNA metabarcoding of plants and animals","volume":"21","author":"E Coissac","year":"2012","journal-title":"Mol Ecol"},{"issue":"1","key":"ref76","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3201\/eid1801.110716","article-title":"Colpodella spp.\u2013like parasite infection in woman, China","volume":"18","author":"CL Yuan","year":"2012","journal-title":"Emerg Infect Dis"},{"issue":"3","key":"ref77","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S1434-4610(98)70031-8","article-title":"Diversity and geographic distribution of free-living heterotrophic flagellates\u2014Analysis by PRIMER","volume":"149","author":"WJ Lee","year":"1998","journal-title":"Protist"},{"key":"ref78","first-page":"519","article-title":"Adaptation to life at high salt concentrations in Archaea, Bacteria, and Eukarya. Cellular origin, life in extreme habitats and astrobiology","volume":"vol. 9","author":"G. Hauer","year":"2005"},{"issue":"1","key":"ref79","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1128\/CMR.12.1.147","article-title":"Antiseptics and disinfectants: activity, action, and resistance","volume":"12","author":"G McDonnell","year":"1999","journal-title":"Clin Microbiol Rev"},{"issue":"8","key":"ref80","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1128\/AEM.01803-07","article-title":"Improved methodology for identification of protists and microalgae from plankton samples preserved in lugol\u2019s iodine solution: combining microscopic analysis with single-cell PCR","volume":"74","author":"BM Auinger","year":"2008","journal-title":"Appl Environ Microbiol"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0005790","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T00:00:00Z","timestamp":1501804800000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0005790","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T01:02:40Z","timestamp":1569891760000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0005790"}},"subtitle":[],"editor":[{"given":"Carlos A.","family":"Buscaglia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,7,20]]},"references-count":80,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0005790","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,20]]}}}