{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:28:26Z","timestamp":1776450506392,"version":"3.51.2"},"update-to":[{"DOI":"10.1371\/journal.pntd.0005886","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T00:00:00Z","timestamp":1505952000000}}],"reference-count":90,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2017,9,5]],"date-time":"2017-09-05T00:00:00Z","timestamp":1504569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Leverhulme Trust (GB)","award":["Grant number RL2012-025"],"award-info":[{"award-number":["Grant number RL2012-025"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0005886","type":"journal-article","created":{"date-parts":[[2017,9,5]],"date-time":"2017-09-05T17:46:41Z","timestamp":1504633601000},"page":"e0005886","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues"],"prefix":"10.1371","volume":"11","author":[{"given":"Lindsay B.","family":"Tulloch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie K.","family":"Menzies","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew L.","family":"Fraser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eoin R.","family":"Gould","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth F.","family":"King","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija K.","family":"Zacharova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon J.","family":"Florence","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1994-2009","authenticated-orcid":true,"given":"Terry K.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,9,5]]},"reference":[{"key":"ref1","unstructured":"WHO. Trypanosomiasis, human African (sleeping sickness) Fact Sheet 259. Trypanosomiasis, human African (sleeping sickness) Fact Sheet 259. 2013. p. Retrieved from <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/mediacentre\/fact\" xlink:type=\"simple\">http:\/\/www.who.int\/mediacentre\/fact<\/ext-link>."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nrd2582","article-title":"Paul Ehrlich: founder of chemotherapy","volume":"7","author":"SHE Kaufmann","year":"2008","journal-title":"Nature reviews. Drug discovery"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1159\/000149583","article-title":"The contributions of Paul Ehrlich to pharmacology: a tribute on the occasion of the centenary of his Nobel Prize","volume":"82","author":"F Bosch","year":"2008","journal-title":"Pharmacology"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1756-3305-3-15","article-title":"The development of drugs for treatment of sleeping sickness: a historical review","volume":"3","author":"D Steverding","year":"2010","journal-title":"Parasit Vectors"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S1474-4422(12)70296-X","article-title":"Clinical features, diagnosis, and treatment of human African trypanosomiasis (sleeping sickness)","volume":"12","author":"PGE Kennedy","year":"2013","journal-title":"Lancet Neurol"},{"issue":"5963","key":"ref6","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1016\/S0140-6736(00)88968-0","article-title":"NEW TRYPANOCIDAL SUBSTANCES","volume":"230","author":"H King","year":"1937","journal-title":"Lancet"},{"issue":"3\u20134","key":"ref7","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1080\/00034983.1939.11685073","article-title":"Studies in Chemotherapy","volume":"33","author":"EM Lourie","year":"1939","journal-title":"Ann Trop Med Parasitol"},{"issue":"9709","key":"ref8","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0140-6736(09)60829-1","article-title":"Human African trypanosomiasis","volume":"375","author":"R Brun","year":"2010","journal-title":"Lancet"},{"issue":"3","key":"ref9","first-page":"171","article-title":"Therapeutic failure of melarsoprol among patients treated for late stage T.b. gambiense human African trypanosomiasis in Uganda","volume":"92","author":"D Legros","year":"1999","journal-title":"Bull Soc Pathol Exot"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1046\/j.1365-3156.2001.00712.x","article-title":"Melarsoprol refractory T. b. gambiense from Omugo, north-western Uganda","volume":"6","author":"E Matovu","year":"2001","journal-title":"Trop Med Int Health"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1046\/j.1365-3156.2001.00724.x","article-title":"The situation of sleeping sickness in Angola: a calamity","volume":"6","author":"A Stanghellini","year":"2001","journal-title":"Trop Med Int Health"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"966","DOI":"10.3201\/eid1406.071266","article-title":"High failure rates of melarsoprol for sleeping sickness, Democratic Republic of Congo","volume":"14","author":"J Robays","year":"2008","journal-title":"Emerg Infect Dis"},{"issue":"4","key":"ref13","first-page":"411","article-title":"Development of drug resistance in Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense. Treatment of human African trypanosomiasis with natural products (Review)","volume":"22","author":"S Gehrig","year":"2008","journal-title":"Int J Mol Med"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1086\/649917","article-title":"How to shorten patient follow-up after treatment for Trypanosoma brucei gambiense sleeping sickness","volume":"201","author":"D Mumba Ngoyi","year":"2010","journal-title":"J Infect Dis"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1021\/ol1028699","article-title":"Synthesis and Stereochemical Assignment of (+)-Chamuvarinin","volume":"13","author":"GJ Florence","year":"2011","journal-title":"Org Lett"},{"issue":"25","key":"ref16","doi-asserted-by":"crossref","first-page":"8309","DOI":"10.1002\/chem.201204527","article-title":"Total synthesis, stereochemical assignment, and biological activity of chamuvarinin and structural analogues","volume":"19","author":"GJ Florence","year":"2013","journal-title":"Chem\u2014A Eur J"},{"issue":"11","key":"ref17","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1002\/cmdc.201402272","article-title":"Non-natural Acetogenin Analogues as Potent Trypanosoma brucei Inhibitors","volume":"9","author":"GJ Florence","year":"2014","journal-title":"ChemMedChem"},{"issue":"22","key":"ref18","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1039\/B500186M","article-title":"Acetogenins from Annonaceae: recent progress in isolation, synthesis and mechanisms of action","volume":"1","author":"A Bermejo","year":"2005","journal-title":"Nat Prod Reports"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1002\/ps.2780330405","article-title":"Molecular mode of action of annonins","volume":"33","author":"M Londershausen","year":"1991","journal-title":"Pestic Sci"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1042\/bj3010161","article-title":"Natural substances (acetogenins) from the family Annonaceae are powerful inhibitors of mitochondrial NADH dehydrogenase (Complex I)","volume":"301","author":"M Degli Esposti","year":"1994","journal-title":"Biochem J"},{"issue":"23","key":"ref21","doi-asserted-by":"crossref","first-page":"4794","DOI":"10.1021\/bi100454b","article-title":"Exploring the binding site of delta(lac)-acetogenin in bovine heart mitochondrial NADH-ubiquinone oxidoreductase","volume":"49","author":"N Kakutani","year":"2010","journal-title":"Biochemistry"},{"issue":"20","key":"ref22","doi-asserted-by":"crossref","first-page":"11160","DOI":"10.1021\/jf203017f","article-title":"Nematicidal and antifungal activities of annonaceous acetogenins from <italic>Annona squamosa<\/italic> against various plant pathogens","volume":"59","author":"Kim WK Dang Q Le","year":"2011","journal-title":"J Agric Food Chem"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Tolosa D, Hidalgo JR, Sal PE, Popich S, Bard\u00f3n A, Neske A. Insecticidal Effects of the Annonaceous Acetogenin Squamocin and the Acetogenin Fraction of Seeds of Rollinia occidentalis on Soybean and Corn Pests. 2014;(November):156\u201360.","DOI":"10.4236\/jacen.2014.34019"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"138","DOI":"10.2174\/1871520615666150629101827","article-title":"Acetogenins as Potential Anticancer Agents","volume":"16","author":"M Mangal","year":"2015","journal-title":"Anticancer Agents Med Chem"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1016\/j.bmcl.2012.02.109","article-title":"Antitumor activity of annonaceous acetogenins in HepS and S180 xenografts bearing mice","volume":"22","author":"Y Chen","year":"2012","journal-title":"Bioorg Med Chem Lett"},{"issue":"9","key":"ref26","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.1046\/j.1432-1327.2000.01263.x","article-title":"Design syntheses and mitochondrial complex I inhibitory activity of novel acetogenin mimics","volume":"267","author":"K Kuwabara","year":"2000","journal-title":"Eur J Biochem"},{"issue":"2\u20133","key":"ref27","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/S0005-2728(00)00156-0","article-title":"Definition of crucial structural factors of acetogenins, potent inhibitors of mitochondrial complex I","volume":"1460","author":"M Takada","year":"2000","journal-title":"Biochim Biophys Acta"},{"issue":"5","key":"ref28","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/j.bmcl.2013.01.018","article-title":"Critical role of a methyl group on the gamma-lactone ring of annonaceous acetogenins in the potent inhibition of mitochondrial complex I","volume":"23","author":"N Kojima","year":"2013","journal-title":"Bioorg Med Chem Lett"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.molbiopara.2010.11.003","article-title":"Complex i (NADH:ubiquinone oxidoreductase) is active in but non-essential for procyclic Trypanosoma brucei","volume":"175","author":"Z Verner","year":"2011","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1128\/EC.05282-11","article-title":"Enigmatic presence of mitochondrial complex I in Trypanosoma brucei bloodstream forms","volume":"11","author":"S Surve","year":"2012","journal-title":"Eukaryot Cell"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4155\/fmc.14.152","article-title":"Photoaffinity labeling in target- and binding-site identification","volume":"7","author":"E Smith","year":"2015","journal-title":"Future Med Chem"},{"issue":"20","key":"ref32","doi-asserted-by":"crossref","first-page":"14280","DOI":"10.1074\/jbc.274.20.14280","article-title":"Photoaffinity labeling and mass spectrometry identify ribosomal protein S3 as a potential target for hybrid polar cytodifferentiation agents","volume":"274","author":"Y Webb","year":"1999","journal-title":"J Biol Chem"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"e61551","DOI":"10.1371\/journal.pone.0061551","article-title":"Identification of a mitochondrial target of thiazolidinedione insulin sensitizers (mTOT)\u2014relationship to newly identified mitochondrial pyruvate carrier proteins","volume":"8","author":"JR Colca","year":"2013","journal-title":"PLoS One"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1021\/bc015588t","article-title":"Identification of two tamoxifen target proteins by photolabeling with 4-(2-morpholinoethoxy)benzophenone","volume":"13","author":"F Mesange","year":"2002","journal-title":"Bioconjug Chem"},{"issue":"5","key":"ref35","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1158\/1535-7163.MCT-04-0333","article-title":"The discovery and mechanism of action of novel tumor-selective and apoptosis-inducing 3,5-diaryl-1,2,4-oxadiazole series using a chemical genetics approach","volume":"4","author":"KA Jessen","year":"2005","journal-title":"Mol Cancer Ther"},{"issue":"5","key":"ref36","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1124\/mol.113.090985","article-title":"Identifying barbiturate binding sites in a nicotinic acetylcholine receptor with [3H]allyl m-trifluoromethyldiazirine mephobarbital, a photoreactive barbiturate","volume":"85","author":"AK Hamouda","year":"2014","journal-title":"Mol Pharmacol"},{"issue":"6787","key":"ref37","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/35015085","article-title":"Photoactivated gamma-secretase inhibitors directed to the active site covalently label presenilin 1","volume":"405","author":"YM Li","year":"2000","journal-title":"Nature"},{"issue":"9","key":"ref38","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1038\/nchembio.2007.16","article-title":"Splicing factor SF3b as a target of the antitumor natural product pladienolide","volume":"3","author":"Y Kotake","year":"2007","journal-title":"Nat Chem Biol"},{"issue":"9","key":"ref39","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.chembiol.2009.07.010","article-title":"Chemical probes identify a role for histone deacetylase 3 in Friedreich\u2019s ataxia gene silencing","volume":"16","author":"C Xu","year":"2009","journal-title":"Chem Biol"},{"issue":"34","key":"ref40","doi-asserted-by":"crossref","first-page":"26315","DOI":"10.1074\/jbc.M110.104547","article-title":"Identification of a substrate-binding site in a peroxisomal beta-oxidation enzyme by photoaffinity labeling with a novel palmitoyl derivative","volume":"285","author":"Y Kashiwayama","year":"2010","journal-title":"J Biol Chem"},{"issue":"9","key":"ref41","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1016\/j.bmc.2010.03.037","article-title":"Anti-microtubule \u201cplinabulin\u201d chemical probe KPU-244-B3 labeled both alpha- and beta-tubulin","volume":"18","author":"Y Yamazaki","year":"2010","journal-title":"Bioorg Med Chem"},{"issue":"24","key":"ref42","doi-asserted-by":"crossref","first-page":"7559","DOI":"10.1016\/j.bmc.2011.10.027","article-title":"Photoaffinity labeling of Ras converting enzyme using peptide substrates that incorporate benzoylphenylalanine (Bpa) residues: improved labeling and structural implications","volume":"19","author":"K Kyro","year":"2011","journal-title":"Bioorg Med Chem"},{"issue":"9","key":"ref43","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1016\/j.bmcl.2013.02.098","article-title":"Characterization of the receptor binding residues of kisspeptins by positional scanning using peptide photoaffinity probes","volume":"23","author":"R Misu","year":"2013","journal-title":"Bioorg Med Chem Lett"},{"issue":"14","key":"ref44","doi-asserted-by":"crossref","first-page":"9710","DOI":"10.1074\/jbc.M112.398602","article-title":"gamma-Secretase modulator (GSM) photoaffinity probes reveal distinct allosteric binding sites on presenilin","volume":"288","author":"N Pozdnyakov","year":"2013","journal-title":"J Biol Chem"},{"issue":"47","key":"ref45","doi-asserted-by":"crossref","first-page":"14560","DOI":"10.1021\/ja076250y","article-title":"Photo-leucine incorporation reveals the target of a cyclodepsipeptide inhibitor of cotranslational translocation","volume":"129","author":"AL MacKinnon","year":"2007","journal-title":"J Am Chem Soc"},{"issue":"30","key":"ref46","doi-asserted-by":"crossref","first-page":"10777","DOI":"10.1021\/ja505517t","article-title":"Mapping the protein interaction landscape for fully functionalized small-molecule probes in human cells","volume":"136","author":"T Kambe","year":"2014","journal-title":"J Am Chem Soc"},{"issue":"24","key":"ref47","doi-asserted-by":"crossref","first-page":"8669","DOI":"10.1021\/ja502372j","article-title":"Chemoproteomic profiling of lysine acetyltransferases highlights an expanded landscape of catalytic acetylation","volume":"136","author":"DC Montgomery","year":"2014","journal-title":"J Am Chem Soc"},{"issue":"6","key":"ref48","doi-asserted-by":"crossref","first-page":"3001","DOI":"10.1021\/ja208518u","article-title":"Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes","volume":"134","author":"H Shi","year":"2012","journal-title":"J Am Chem Soc"},{"issue":"39","key":"ref49","doi-asserted-by":"crossref","first-page":"10286","DOI":"10.1002\/anie.201304987","article-title":"Identification of malate dehydrogenase 2 as a target protein of the HIF-1 inhibitor LW6 using chemical probes","volume":"52","author":"K Lee","year":"2013","journal-title":"Angew Chem Int Ed Engl"},{"issue":"11","key":"ref50","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1002\/cbic.200300705","article-title":"Azide-Alkyne Coupling: A Powerful Reaction for Bioconjugate Chemistry","volume":"4","author":"R Breinbauer","year":"2003","journal-title":"ChemBioChem"},{"issue":"4","key":"ref51","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1111\/j.1550-7408.1972.tb03561.x","article-title":"The utilization of glucose and proline by culture forms of Trypanosoma brucei","volume":"19","author":"DA Evans","year":"1972","journal-title":"J Protozool"},{"issue":"2","key":"ref52","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0035-9203(73)90157-0","article-title":"Metabolism of proline by the culture midgut form of Trypanosoma rhodesiense","volume":"67","author":"WC Ford","year":"1973","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"349","DOI":"10.2741\/3931","article-title":"Multiple roles of proline transport and metabolism in trypanosomatids","volume":"17","author":"F Bringaud","year":"2012","journal-title":"Front Biosci"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0166-6851(00)00303-0","article-title":"ATP production in isolated mitochondria of procyclic Trypanosoma brucei","volume":"111","author":"N Allemann","year":"2000","journal-title":"Mol Biochem Parasitol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/978-1-59745-365-3_27","article-title":"ATP production in isolated mitochondria of procyclic Trypanosoma brucei","volume":"372","author":"A Schneider","year":"2007","journal-title":"Methods Mol Biol"},{"issue":"2","key":"ref56","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1042\/bj2590363","article-title":"The role of succinate in the respiratory chain of Trypanosoma brucei procyclic trypomastigotes","volume":"259","author":"JF Turrens","year":"1989","journal-title":"Biochem J"},{"issue":"12","key":"ref57","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1177\/44.12.8985128","article-title":"Analysis of mitochondrial morphology and function with novel fixable fluorescent stains","volume":"44","author":"M Poot","year":"1996","journal-title":"J Histochem Cytochem"},{"issue":"2","key":"ref58","doi-asserted-by":"crossref","first-page":"98","DOI":"10.2144\/000113610","article-title":"Gradient: a Practical Usage Guide","volume":"50","author":"SW Perry","year":"2011","journal-title":"Biotechniques"},{"issue":"2","key":"ref59","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/cyto.a.20033","article-title":"Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues","volume":"61","author":"W Pendergrass","year":"2004","journal-title":"Cytometry A"},{"issue":"11","key":"ref60","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1128\/EC.4.11.1942-1950.2005","article-title":"Highly Efficient Tandem Affinity Purification of Trypanosome Protein Complexes Based on a Novel Epitope Combination Highly Efficient Tandem Affinity Purification of Trypanosome Protein Complexes Based on a Novel Epitope Combination","volume":"4","author":"B Schimanski","year":"2005","journal-title":"Eukaryot Cell"},{"issue":"5","key":"ref61","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000436","article-title":"The F0F1-ATP synthase complex contains novel subunits and is essential for procyclic Trypanosoma brucei","volume":"5","author":"A Z\u00edkov\u00e1","year":"2009","journal-title":"PLoS Pathog"},{"issue":"5","key":"ref62","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1073\/pnas.1612386114","article-title":"In situ structure of trypanosomal ATP synthase dimer reveals a unique arrangement of catalytic subunits","volume":"114","author":"AW Muhleip","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6491","key":"ref63","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/370621a0","article-title":"Structure at 2.8 A resolution of F1-ATPase from bovine heart mitochondria","volume":"370","author":"JP Abrahams","year":"1994","journal-title":"Nature"},{"issue":"5445","key":"ref64","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1126\/science.286.5445.1700","article-title":"Molecular architecture of the rotary motor in ATP synthase","volume":"286","author":"D Stock","year":"1999","journal-title":"Science"},{"issue":"24","key":"ref65","doi-asserted-by":"crossref","first-page":"9585","DOI":"10.1073\/pnas.87.24.9585","article-title":"Ligand-dependent structural variations in Escherichia coli F1 ATPase revealed by cryoelectron microscopy","volume":"87","author":"EP Gogol","year":"1990","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6622","key":"ref66","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/386299a0","article-title":"Direct observation of the rotation of F1-ATPase","volume":"386","author":"H Noji","year":"1997","journal-title":"Nature"},{"issue":"11","key":"ref67","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1038\/nchembio.1635","article-title":"Chemomechanical coupling of human mitochondrial F1-ATPase motor","volume":"10","author":"T Suzuki","year":"2014","journal-title":"Nat Chem Biol"},{"issue":"38","key":"ref68","doi-asserted-by":"crossref","first-page":"29502","DOI":"10.1074\/jbc.M110.124529","article-title":"Crystal structure of the Mg.ADP-inhibited state of the yeast F1c10-ATP synthase","volume":"285","author":"A Dautant","year":"2010","journal-title":"J Biol Chem"},{"issue":"2","key":"ref69","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"O Trott","year":"2010","journal-title":"J Comput Chem"},{"issue":"8","key":"ref70","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1002\/j.1460-2075.1982.tb01276.x","article-title":"Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold","volume":"1","author":"JE Walker","year":"1982","journal-title":"EMBO J. England"},{"issue":"10","key":"ref71","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1093\/protein\/15.10.783","article-title":"A conformational analysis of Walker motif A [GXXXXGKT (S)] in nucleotide-binding and other proteins","volume":"15","author":"C Ramakrishnan","year":"2002","journal-title":"Protein Eng"},{"issue":"2","key":"ref72","first-page":"1","article-title":"Targeting Energy Metabolism in Mycobacterium tuberculosis, a New Paradigm in Antimycobacterial Drug Discovery","volume":"8","author":"D Bald","year":"2017","journal-title":"Am J Microbiol"},{"issue":"23","key":"ref73","doi-asserted-by":"crossref","first-page":"4029","DOI":"10.1038\/sj.emboj.7600862","article-title":"The F1-ATP synthase complex in bloodstream stage trypanosomes has an unusual and essential function","volume":"24","author":"A Schnaufer","year":"2005","journal-title":"EMBO J"},{"issue":"2","key":"ref74","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.ppat.1004660","article-title":"ATPaseTb2, a Unique Membrane-bound FoF1-ATPase Component, Is Essential in Bloodstream and Dyskinetoplastic Trypanosomes","volume":"11","author":"K \u0160ubrtov\u00e1","year":"2015","journal-title":"PLoS Pathog"},{"issue":"21","key":"ref75","doi-asserted-by":"crossref","first-page":"13034","DOI":"10.1016\/S0021-9258(18)33618-4","article-title":"Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria","volume":"257","author":"SM Gasser","year":"1982","journal-title":"J Biol Chem"},{"issue":"9","key":"ref76","doi-asserted-by":"crossref","first-page":"6253","DOI":"10.1128\/MCB.19.9.6253","article-title":"In vitro import of a nuclearly encoded tRNA into the mitochondrion of Trypanosoma brucei","volume":"19","author":"AE Yermovsky-Kammerer","year":"1999","journal-title":"Mol Cell Biol"},{"issue":"1","key":"ref77","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1475-9292-2-11","article-title":"Evolution of energy metabolism and its compartmentation in Kinetoplastida","volume":"2","author":"V Hannaert","year":"2003","journal-title":"Kinetoplastid Biol Dis."},{"key":"ref78","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1146\/annurev.mi.41.100187.001015","article-title":"Compartmentation of carbohydrate metabolism in trypanosomes","volume":"41","author":"FR Opperdoes","year":"1987","journal-title":"Annu Rev Microbiol"},{"issue":"1","key":"ref79","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1432-1033.1992.tb17278.x","article-title":"The mitochondrion in bloodstream forms of Trypanosoma brucei is energized by the electrogenic pumping of protons catalysed by the F1F0-ATPase","volume":"209","author":"DP Nolan","year":"1992","journal-title":"Eur J Biochem"},{"issue":"1","key":"ref80","first-page":"44","article-title":"ATP Synthase Is Responsible for Maintaining Mitochondrial Membrane Potential in Bloodstream Form Trypanosoma brucei ATP Synthase Is Responsible for Maintaining Mitochondrial Membrane Potential in Bloodstream Form Trypanosoma brucei","volume":"5","author":"V Brown S","year":"2006","journal-title":"Society"},{"issue":"1979","key":"ref81","first-page":"289","article-title":"Sch\u00f6nenberger. Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication","volume":"36","author":"R Brun","year":"1979","journal-title":"Acta Trop"},{"issue":"1","key":"ref82","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0166-6851(99)00002-X","article-title":"A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei","volume":"99","author":"E Wirtz","year":"1999","journal-title":"Mol Biochem Parasitol"},{"issue":"45","key":"ref83","doi-asserted-by":"crossref","first-page":"35224","DOI":"10.1074\/jbc.M110.165860","article-title":"The dithiol glutaredoxins of African trypanosomes have distinct roles and are closely linked to the unique trypanothione metabolism","volume":"285","author":"S Ceylan","year":"2010","journal-title":"J Biol Chem"},{"issue":"2","key":"ref84","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/nmeth.1293","article-title":"Large-Scale Profiling of Protein Palmitoylation in Mammalian Cells","volume":"6","author":"BR Martin","year":"2009","journal-title":"Nat Methods"},{"key":"ref85","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jchromb.2014.07.023","article-title":"Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS\/MS analysis","volume":"967","author":"P Podolec","year":"2014","journal-title":"J Chromatogr B Anal Technol Biomed Life Sci"},{"issue":"36","key":"ref86","doi-asserted-by":"crossref","first-page":"22983","DOI":"10.1074\/jbc.273.36.22983","article-title":"Functional F1-ATPase essential in maintaining growth and membrane potential of human mitochondrial DNA-depleted rho degrees cells","volume":"273","author":"K Buchet","year":"1998","journal-title":"J Biol Chem"},{"issue":"6","key":"ref87","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1002\/j.1939-4640.2000.tb03420.x","article-title":"The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation","volume":"21","author":"J Baumber","year":"2000","journal-title":"J Androl. United States"},{"issue":"17","key":"ref88","doi-asserted-by":"crossref","first-page":"9505","DOI":"10.1073\/pnas.181319898","article-title":"Mitochondria play no roles in Mn(II)-induced apoptosis in HeLa cells","volume":"98","author":"H Oubrahim","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref89","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.cbi.2008.07.002","article-title":"Aclarubicin-induced ROS generation and collapse of mitochondrial membrane potential in human cancer cell lines","volume":"176","author":"A Rogalska","year":"2008","journal-title":"Chem Biol Interact"},{"issue":"16","key":"ref90","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility","volume":"30","author":"GM Morris","year":"2009","journal-title":"J Comput Chem"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0005886","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T00:00:00Z","timestamp":1505952000000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0005886","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T18:44:37Z","timestamp":1719427477000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0005886"}},"subtitle":[],"editor":[{"given":"Walderez O.","family":"Dutra","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,9,5]]},"references-count":90,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2017,9,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0005886","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0005886","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,5]]}}}