{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:32:22Z","timestamp":1792690342233,"version":"4.5.0"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T00:00:00Z","timestamp":1516233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["SON15001"],"award-info":[{"award-number":["SON15001"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0006130","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T18:28:13Z","timestamp":1516300093000},"page":"e0006130","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":53,"title":["A comparative analysis of preservation techniques for the optimal molecular detection of hookworm DNA in a human fecal specimen"],"prefix":"10.1371","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9012-2703","authenticated-orcid":true,"given":"Marina","family":"Papaiakovou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Pilotte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Baumer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Grant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristjana","family":"Asbjornsdottir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Schaer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffi","family":"Aroian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judd","family":"Walson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,1,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jviromet.2003.10.003","article-title":"Comparison of storage methods for reverse-transcriptase PCR amplification of rotavirus RNA from gorilla (Gorilla g. gorilla) fecal samples","volume":"116","author":"CA Whittier","year":"2004","journal-title":"J Virol Methods"},{"key":"ref2","article-title":"Fecal Bacteria Act as Novel Biomarkers for Noninvasive Diagnosis of Colorectal Cancer","author":"Q Liang","year":"2016","journal-title":"Clin Cancer Res Off J Am Assoc Cancer Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e331","DOI":"10.1371\/journal.pntd.0000331","article-title":"Diagnosis of Soil-Transmitted Helminths in the Era of Preventive Chemotherapy: Effect of Multiple Stool Sampling and Use of Different Diagnostic Techniques","volume":"2","author":"S Knopp","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e0004578","DOI":"10.1371\/journal.pntd.0004578","article-title":"Improved PCR-Based Detection of Soil Transmitted Helminth Infections Using a Next-Generation Sequencing Approach to Assay Design","volume":"10","author":"N Pilotte","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e0005734","DOI":"10.1371\/journal.pntd.0005734","article-title":"A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite <italic>Ancylostoma ceylanicum<\/italic> in human stool","volume":"11","author":"M Papaiakovou","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1097\/01.pas.0000176768.18423.7e","article-title":"DNA stabilization is critical for maximizing performance of fecal DNA-based colorectal cancer tests","volume":"14","author":"J Olson","year":"2005","journal-title":"Diagn Mol Pathol Am J Surg Pathol"},{"key":"ref7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0126935","article-title":"Differential Nuclear and Mitochondrial DNA Preservation in Post-Mortem Teeth with Implications for Forensic and Ancient DNA Studies","volume":"10","author":"D Higgins","year":"2015","journal-title":"PLoS ONE"},{"key":"ref8","article-title":"Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field Studies","volume":"1","author":"SJ Song","year":"2016","journal-title":"MSystems"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1128\/JCM.32.12.3072-3074.1994","article-title":"Comparison of three stool-processing methods for detection of <italic>Salmonella<\/italic> serogroups B, C2, and D by PCR","volume":"32","author":"U Kongmuang","year":"1994","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"4763","DOI":"10.1128\/JCM.40.12.4763-4764.2002","article-title":"Rotavirus Particles Can Survive Storage in Ambient Tropical Temperatures for More than 2 Months","volume":"40","author":"TK Fischer","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.vetpar.2016.03.012","article-title":"Testing storage methods of faecal samples for subsequent measurement of helminth egg numbers in the domestic horse","volume":"221","author":"JAH Crawley","year":"2016","journal-title":"Vet Parasitol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.trstmh.2007.09.009","article-title":"FLOTAC: a new sensitive technique for the diagnosis of hookworm infections in humans","volume":"102","author":"J Utzinger","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e754","DOI":"10.1371\/journal.pntd.0000754","article-title":"Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for <italic>Schistosoma mansoni<\/italic> and soil-transmitted helminths","volume":"4","author":"D Glinz","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref14","author":"C Wang","year":"2012"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","article-title":"PCR inhibitors\u2014occurrence, properties and removal","volume":"113","author":"C Schrader","year":"2012","journal-title":"J Appl Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1590\/S0074-02762012000800001","article-title":"Stool sample storage conditions for the preservation of <italic>Giardia intestinalis<\/italic> DNA","volume":"107","author":"S Kuk","year":"2012","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.mimet.2009.06.018","article-title":"Preservation of <italic>Giardia<\/italic> cysts in stool samples for subsequent PCR analysis","volume":"78","author":"H Wilke","year":"2009","journal-title":"J Microbiol Methods"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e0004380","DOI":"10.1371\/journal.pntd.0004380","article-title":"Application of a Multiplex Quantitative PCR to Assess Prevalence and Intensity Of Intestinal Parasite Infections in a Controlled Clinical Trial","volume":"10","author":"S Llewellyn","year":"2016","journal-title":"PLOS Negl Trop Dis"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1002\/ajp.20351","article-title":"Molecular analyses of the intestinal microbiota of chimpanzees in the wild and in captivity","volume":"69","author":"G Uenishi","year":"2007","journal-title":"Am J Primatol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.actatropica.2017.03.019","article-title":"Epidemiology of polyparasitism with <italic>Taenia solium<\/italic>, schistosomes and soil-transmitted helminths in the co-endemic village of Malanga, Democratic Republic of Congo","volume":"171","author":"J Madinga","year":"2017","journal-title":"Acta Trop"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.trstmh.2007.10.011","article-title":"Multiplex real-time PCR for the detection and quantification of <italic>Schistosoma mansoni<\/italic> and <italic>S<\/italic>. <italic>haematobium<\/italic> infection in stool samples collected in northern Senegal","volume":"102","author":"RJ ten Hove","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s00040-003-0709-x","article-title":"Recommendations on the use of alcohols for preservation of ant specimens (<italic>Hymenoptera<\/italic>, <italic>Formicidae<\/italic>)","volume":"51","author":"JR King","year":"2004","journal-title":"Insectes Sociaux"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1002\/ajp.20961","article-title":"Analysis of the hydrogenotrophic microbiota of wild and captive black howler monkeys (<italic>Alouatta pigra<\/italic>) in palenque national park, Mexico","volume":"73","author":"N Nakamura","year":"2011","journal-title":"Am J Primatol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1017\/S0031182017000129","article-title":"Improved diagnosis of <italic>Trichuris trichiura<\/italic> by using a bead-beating procedure on ethanol preserved stool samples prior to DNA isolation and the performance of multiplex real-time PCR for intestinal parasites","volume":"144","author":"MMM Kaisar","year":"2017","journal-title":"Parasitology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e0005310","DOI":"10.1371\/journal.pntd.0005310","article-title":"Diagnosing Polyparasitism in a High-Prevalence Setting in Beira, Mozambique: Detection of Intestinal Parasites in Fecal Samples by Microscopy and Real-Time PCR","volume":"11","author":"L Meurs","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"685","DOI":"10.4269\/ajtmh.2007.77.685","article-title":"Simultaneous detection and quantification of <italic>Ancylostoma duodenale<\/italic>, <italic>Necator americanus<\/italic>, and <italic>Oesophagostomum bifurcum<\/italic> in fecal samples using multiplex real-time PCR","volume":"77","author":"JJ Verweij","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"16350","DOI":"10.1038\/srep16350","article-title":"Sample storage conditions significantly influence faecal microbiome profiles","volume":"5","author":"JM Choo","year":"2015","journal-title":"Sci Rep"},{"key":"ref28","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0134802","article-title":"Methods for Improving Human Gut Microbiome Data by Reducing Variability through Sample Processing and Storage of Stool","volume":"10","author":"MA Gorzelak","year":"2015","journal-title":"PLoS ONE"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1158\/1055-9965.EPI-07-2515","article-title":"Exfoliated cells in stool: a source for reverse transcription-PCR-based analysis of biomarkers of gastrointestinal cancer","volume":"17","author":"YJ Yu","year":"2008","journal-title":"Cancer Epidemiol Biomark Prev Publ Am Assoc Cancer Res Cosponsored Am Soc Prev Oncol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1111\/j.1365-294X.2004.02207.x","article-title":"Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method","volume":"13","author":"AM Nsubuga","year":"2004","journal-title":"Mol Ecol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.mimet.2007.11.007","article-title":"Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces","volume":"72","author":"JM Nechvatal","year":"2008","journal-title":"J Microbiol Methods"},{"key":"ref32","author":"DD Bowman","year":"2008"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1111\/j.1472-765X.2010.02804.x","article-title":"A versatile internal control for use as DNA in real-time PCR and as RNA in real-time reverse transcription PCR assays","volume":"50","author":"DM Deer","year":"2010","journal-title":"Lett Appl Microbiol"},{"key":"ref34","author":"H Wickham","year":"2009"},{"key":"ref35","author":"JJ Faraway","year":"2016"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.mcp.2007.05.006","article-title":"Real-time PCR for the detection of <italic>Dientamoeba fragilis<\/italic> in fecal samples","volume":"21","author":"JJ Verweij","year":"2007","journal-title":"Mol Cell Probes"},{"key":"ref37","article-title":"The effect of tropical ambient temperature on productive performance and grazing behavior of sows kept in an outdoor system","volume":"12","author":"RHS Ricalde","year":"2000","journal-title":"Livest Res Rural Dev"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1017\/S0950268804003024","article-title":"Spatial analysis of the distribution of intestinal nematode infections in Uganda","volume":"132","author":"S Brooker","year":"2004","journal-title":"Epidemiol Infect"},{"key":"ref39","doi-asserted-by":"crossref","DOI":"10.1186\/s12864-015-1694-y","article-title":"Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation","volume":"16","author":"M Reck","year":"2015","journal-title":"BMC Genomics"},{"key":"ref40","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-016-1657-4","article-title":"The level of embryonation influences detection of <italic>Ostertagia ostertagi<\/italic> eggs by semi-quantitative PCR","volume":"9","author":"M Drag","year":"2016","journal-title":"Parasit Vectors"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.vetpar.2009.09.043","article-title":"Effects of fecal collection and storage factors on strongylid egg counts in horses","volume":"167","author":"MK Nielsen","year":"2010","journal-title":"Vet Parasitol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2478\/bvip-2013-0030","article-title":"Effect of Storage and Preservation of Horse Faecal Samples on the Detectability and Viability of Strongylid Nematode Eggs and Larvae","volume":"57","author":"E Jag\u0142a","year":"2013","journal-title":"Bull Vet Inst Pulawy"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/S0099-1767(97)90106-9","article-title":"DNA evidence collection","volume":"23","author":"LE Ledray","year":"1997","journal-title":"J Emerg Nurs JEN Off Publ Emerg Dep Nurses Assoc"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1111\/j.1471-8286.2007.01829.x","article-title":"Ageing and environmental factors affect PCR success in wolf (<italic>Canis lupus<\/italic>) excremental DNA samples","volume":"7","author":"A Santini","year":"2007","journal-title":"Mol Ecol Notes"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1023\/A:1020503330767","article-title":"An evaluation of long-term preservation methods for brown bear (Ursus arctos) faecal DNA samples","volume":"3","author":"MA Murphy","year":"2002","journal-title":"Conserv Genet"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0006130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,25]],"date-time":"2020-10-25T20:43:40Z","timestamp":1603658620000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0006130"}},"subtitle":[],"editor":[{"given":"Cinzia","family":"Cantacessi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,1,18]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,1,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0006130","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,18]]}}}