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Emergency use assessment and listing for Ebola virus disease IVDs, public report, product: Xpert Ebola assay. [Accessed Apr 2017]. Available from: http:\/\/www.who.int\/diagnosticslaboratory\/procurement\/purchasing\/en\/<\/ext-link>. 2015."},{"key":"ref35","unstructured":"R. Carter and L. Van Niekerk. Social Innovation in Health Initiative\u2014Riders for Health. Available at: https:\/\/socialinnovationinhealth.org\/case-studies\/riders-for-health-2\/<\/ext-link>. 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Available from: http:\/\/www.who.int\/mediacentre\/news\/statements\/2015\/liberia-ends-ebola\/en\/<\/ext-link> 2015."},{"issue":"suppl_3","key":"ref39","doi-asserted-by":"crossref","first-page":"S169","DOI":"10.1093\/infdis\/jiw216","article-title":"Ebola Laboratory Response at the Eternal Love Winning Africa Campus, Monrovia, Liberia, 2014\u20132015","volume":"214","author":"E de Wit","year":"2016","journal-title":"The Journal of Infectious Diseases"},{"key":"ref40","unstructured":"World Health Organization. WHO Statement. Ebola transmission in Liberia over, Nation enters 90-day intensive surveillance period [Accessed Oct 2017]. Available from: http:\/\/www.who.int\/mediacentre\/news\/statements\/2015\/ebola-transmission-over-liberia\/en\/<\/ext-link>. 2015."},{"key":"ref41","unstructured":"World Health Organization. WHO Ebola Situation Report 25th November 2015. [Accessed Apr 2017]. Available from: http:\/\/apps.who.int\/ebola\/current-situation\/ebola-situation-report-25-november-2015<\/ext-link> 2015."},{"key":"ref42","unstructured":"World Health Organization. WHO Situation report Zika Virus Disease, Yellow Fever, Ebola Virus Disease, 28 April 2016. [Accessed May 2017]. Available from: http:\/\/apps.who.int\/iris\/bitstream\/10665\/205686\/1\/WHOsitrep_28Apr2016_eng.pdf?ua=1<\/ext-link>. 2016."},{"issue":"513","key":"ref43","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/513145a","article-title":"Make diagnostic centres a priority for Ebola crisis","author":"JD Kelly","year":"2014","journal-title":"Nature"},{"issue":"22","key":"ref44","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.1056\/NEJMoa1411680","article-title":"Clinical Illness and Outcomes in Patients with Ebola in Sierra Leone","volume":"371","author":"JS Schieffelin","year":"2014","journal-title":"New England Journal of Medicine"},{"key":"ref45","unstructured":"World Health Organization. Emergency guidance, selection and use of Ebola in vitro diagnostic (IVD) assays. [Accessed Apr 2017]. 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