{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T20:33:29Z","timestamp":1779309209241,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0006264","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000}}],"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T00:00:00Z","timestamp":1518566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federal Ministry of education and Research\/KfW, Germany","award":["202060457"],"award-info":[{"award-number":["202060457"]}]},{"name":"Ministry of Foreign Affairs, Government of the Netherlands","award":["22211"],"award-info":[{"award-number":["22211"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0006264","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T13:27:06Z","timestamp":1518614826000},"page":"e0006264","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":60,"title":["Sensitive and less invasive confirmatory diagnosis of visceral leishmaniasis in Sudan using loop-mediated isothermal amplification (LAMP)"],"prefix":"10.1371","volume":"12","author":[{"given":"Maowia","family":"Mukhtar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sababil S.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salah A.","family":"Boshara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Albertini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9verine","family":"Monnerat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Bessell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyoshi","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Kubota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph M.","family":"Ndung\u2019u","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2200-2360","authenticated-orcid":true,"given":"Israel","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,2,14]]},"reference":[{"key":"ref1","article-title":"Control of the leishmaniasis: report of a meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva 22\u201326 March 2010. WHO technical report series No. 949","year":"2010"},{"key":"ref2","first-page":"285","article-title":"Leishmaniasis in high-burden countries: an epidemiological update based on data reported in 2014","volume":"91","year":"2016","journal-title":"Weekly Epidemiological Record"},{"key":"ref3","unstructured":"World Health Organization (2015) Visceral leishmaniasis: control strategies and epidemiological situation update in East Africa: report of a WHO bi-regional consultation Addis Ababa, Ethiopia, 9\u201311 March 2015. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/apps.who.int\/iris\/handle\/10665\/190168\" xlink:type=\"simple\">http:\/\/apps.who.int\/iris\/handle\/10665\/190168<\/ext-link>. Accessed 15 February 2017."},{"key":"ref4","first-page":"470","article-title":"Challenges in the Diagnosis of Visceral Leishmaniasis in Sudan","volume":"8","author":"E Abass","year":"2016","journal-title":"International Journal of Collaborative Research on Internal Medicine & Public Health"},{"key":"ref5","unstructured":"Federal Ministry of Health, Republic of Sudan, Neglected Tropical Disease Division (2014) Manual for the diagnosis and treatment of leishmaniasis. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.sudanap.org\/gp7.html\" xlink:type=\"simple\">http:\/\/www.sudanap.org\/gp7.html<\/ext-link>. Accessed 15 February 2017."},{"issue":"Suppl 1","key":"ref6","doi-asserted-by":"crossref","first-page":"S27","DOI":"10.1016\/S0035-9203(01)90218-4","article-title":"Leishmaniasis in Sudan. Visceral leishmaniasis","volume":"95","author":"EE Zijlstra","year":"2001","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1128\/CVI.00473-10","article-title":"Persistence of <italic>Leishmania donovani<\/italic> antibodies in past visceral leishmaniasis cases in India","volume":"18","author":"K Gidwani","year":"2011","journal-title":"Clin Vaccine Immunol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1086\/518167","article-title":"Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review of the literature","volume":"44","author":"S Antinori","year":"2007","journal-title":"Clin Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1128\/JCM.00372-14","article-title":"Molecular tools for diagnosis of visceral leishmaniasis: systematic review and meta-analysis of diagnostic test accuracy","volume":"52","author":"CM de Ruiter","year":"2014","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.4269\/ajtmh.2009.09-0285","article-title":"Short report: Contribution of quantitative real-time polymerase chain reaction to follow-up of visceral leishmaniasis patients treated with meglumine antimoniate","volume":"81","author":"K Aoun","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.actatropica.2009.09.005","article-title":"PCR for diagnosis and assessment of cure in kala-azar patients in Bangladesh","volume":"113","author":"MA Salam","year":"2010","journal-title":"Acta Trop"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"T Notomi","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12275-015-4656-9","article-title":"Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects","volume":"53","author":"T Notomi","year":"2015","journal-title":"J Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1186\/s13071-017-2120-x","article-title":"Diagnostic antigens for visceral leishmaniasis: clarification of nomenclatures","volume":"10","author":"T Bhattacharyya","year":"2017","journal-title":"Parasit Vectors"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/0035-9203(87)90423-8","article-title":"Evaluation of a newly developed direct agglutination test (DAT) for serodiagnosis and seroepidemiological studies of visceral leishmaniasis: comparison of IFAT and ELISA","volume":"81","author":"AE Harith","year":"1987","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1590\/S1020-49892010000100012","article-title":"The Epidat program","volume":"27","author":"MI Santiago P\u00e9rez","year":"2010","journal-title":"Rev Panam Salud Publica"},{"key":"ref17","unstructured":"World Health Organization. The use of loop-mediated isothermal amplification (TB-LAMP) for the diagnosis of pulmonary tuberculosis: policy guidance. WHO\/HTM\/TB\/2016.07. Available at: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/apps.who.int\/iris\/bitstream\/10665\/249154\/1\/9789241511186-eng.pdf\" xlink:type=\"simple\">http:\/\/apps.who.int\/iris\/bitstream\/10665\/249154\/1\/9789241511186-eng.pdf<\/ext-link>. Accessed 15 February 2017."},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s12936-017-1692-4","article-title":"Performance of loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria among malaria suspected pregnant women in Northwest Ethiopia","volume":"16","author":"B Tegegne","year":"2017","journal-title":"Malar J"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"e0171126","DOI":"10.1371\/journal.pone.0171126","article-title":"Development and clinical performance of high throughput loop-mediated isothermal amplification for detection of malaria","volume":"12","author":"RS Perera","year":"2017","journal-title":"PLoS One"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"578","DOI":"10.4269\/ajtmh.2009.09-0145","article-title":"Sensitive, specific, and rapid detection of <italic>Leishmania donovani<\/italic> DNA by loop-mediated isothermal amplification","volume":"81","author":"H Takagi","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2013.12.016","article-title":"Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for <italic>Leishmania<\/italic> infection","volume":"132","author":"CO Nzelu","year":"2014","journal-title":"Acta Trop"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1590\/0074-02760160254","article-title":"Comparison of LAMP and PCR for molecular mass screening of sand flies for <italic>Leishmania martiniquensis<\/italic> infection","volume":"112","author":"S Tiwananthagorn","year":"2017","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1\u20132","key":"ref23","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.vetpar.2013.07.038","article-title":"Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of <italic>Leishmania infantum<\/italic> in canine leishmaniasis based on cysteine protease B genes","volume":"198","author":"M Chaouch","year":"2013","journal-title":"Vet Parasitol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1186\/s13071-015-0991-2","article-title":"Development of a LAMP assay for detection of <italic>Leishmania infantum<\/italic> infection in dogs using conjunctival swab samples","volume":"8","author":"CH Gao","year":"2015","journal-title":"Parasit Vectors"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.actatropica.2016.06.009","article-title":"Optimization of loop-mediated isothermal amplification (LAMP) assays for the detection of <italic>Leishmania<\/italic> DNA in human blood samples","volume":"162","author":"I Abbasi","year":"2016","journal-title":"Acta Trop"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4038\/cmj.v61i2.8286","article-title":"Loop-mediated isothermal amplification assay as a sensitive diagnostic tool for <italic>Leishmania donovani<\/italic> infections in Sri Lanka","volume":"61","author":"HS Kothalawala","year":"2016","journal-title":"Ceylon Med J"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actatropica.2015.10.013","article-title":"A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool","volume":"153","author":"CO Nzelu","year":"2016","journal-title":"Acta Trop"},{"issue":"1","key":"ref28","first-page":"50","article-title":"Development and Assessment of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Iran","volume":"9","author":"M Ghasemian","year":"2014","journal-title":"Iran J Parasitol"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.diagmicrobio.2013.01.011","article-title":"Application of loop-mediated isothermal amplification assay for the sensitive and rapid diagnosis of visceral leishmaniasis and post-kala-azar dermal leishmaniasis","volume":"75","author":"S Verma","year":"2013","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1186\/s12879-017-2318-8","article-title":"Development of a rapid loop-mediated isothermal amplification assay for diagnosis and assessment of cure of <italic>Leishmania<\/italic> infection","volume":"17","author":"S Verma","year":"2017","journal-title":"BMC Infect Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1186\/1756-3305-5-280","article-title":"Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of <italic>Leishmania<\/italic> DNA in buffy coat from visceral leishmaniasis patients","volume":"5","author":"MG Khan","year":"2012","journal-title":"Parasit Vectors"},{"issue":"9","key":"ref32","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1093\/trstmh\/trv060","article-title":"Diagnostic accuracy of rK28-based immunochromatographic rapid diagnostic tests for visceral leishmaniasis: a prospective clinical cohort study in Sudan","volume":"109","author":"M Mukhtar","year":"2015","journal-title":"Trans R Soc Trop Med Hyg"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0006264","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0006264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T17:57:57Z","timestamp":1540317477000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0006264"}},"subtitle":[],"editor":[{"given":"Anne-Laure","family":"Ba\u00f1uls","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,2,14]]},"references-count":32,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,2,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0006264","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0006264","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,14]]}}}