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Morphology, in comparison with <italic>A<\/italic>. <italic>glabratus<\/italic>","volume":"15","author":"WL Paraense","year":"1955","journal-title":"Rev Brasil Biol"},{"key":"ref32","first-page":"105","article-title":"Estado atual da sistem\u00e1tica dos planorb\u00eddeos brasileiros","volume":"55","author":"WL Paraense","year":"1975","journal-title":"Arq Mus Nac"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1590\/S0074-02761981000200011","article-title":"<italic>Biomphalaria occidentalis<\/italic> spn from South America (<italic>Mollusca<\/italic>: <italic>Basommatophora<\/italic>: <italic>Pulmonata<\/italic>)","volume":"76","author":"WL Paraense","year":"1981","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1590\/S0074-02761984000400012","article-title":"<italic>Biomphalaria tenagophila guaibensis<\/italic> ssp.n. from southern Brazil and Uruguay (<italic>Pulmonata<\/italic>: <italic>Planorbidae<\/italic>) I: Morphology","volume":"79","author":"WL Paraense","year":"1984","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1590\/S0074-02761988000100001","article-title":"<italic>Biomphalaria kuhniana<\/italic> (Clessin, 1883), planorbid mollusc from South America","volume":"83","author":"WL Paraense","year":"1988","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref36","first-page":"16","article-title":"Effect of light and temperature on shedding of <italic>Schistosoma mansoni<\/italic> cercarie","volume":"7","author":"ER Kuntz","year":"1946","journal-title":"Naval Med Res Inst"},{"key":"ref37","article-title":"Molecular cloning: A laboratory manual","author":"J Sambrook","year":"1989"},{"key":"ref38","first-page":"413","article-title":"Interactive spatial data analysis","author":"TC Bailey","year":"1995"},{"key":"ref39","first-page":"244","article-title":"Local model for spacial analisis","author":"CD Lloyd","year":"2006"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1590\/S0037-86821987000200002","article-title":"Tentativa de avalia\u00e7\u00e4o do programa especial de controle da esquistossomose (PECE) no Estado da Para\u00edba, Brasil","author":"JR Coura","year":"1987","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.actatropica.2008.09.016","article-title":"The effect of the number of stool samples on the observed prevalence and the infection intensity with <italic>Schistosoma mansoni<\/italic> among a population in an area of low transmission","volume":"108","author":"MJ Enk","year":"2008","journal-title":"Acta Trop"},{"issue":"7","key":"ref42","doi-asserted-by":"crossref","first-page":"e0003959","DOI":"10.1371\/journal.pntd.0003959","article-title":"Is PCR the next reference standard for the diagnosis of Schistosoma in stool? 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