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World Health Organization technical report series: 1\u2013186."},{"key":"ref3","year":"2014"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1051\/parasite\/1997042181","article-title":"Epidemiology of human visceral leishmaniasis in Taounate, a northern province of Morocco","volume":"4","author":"N Guessous-Idrissi","year":"1997","journal-title":"Parasite"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1051\/parasite\/1998054325","article-title":"Canine leishmaniasis due to Leishmania infantum MON-1 in northern Morocco","volume":"5","author":"R Nejjar","year":"1998","journal-title":"Parasite"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1051\/parasite\/199166396","article-title":"Leishmania tropica au Maroc. 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Leishmania Taxonomie et phylogen\u00e8se Application \u00e9co-\u00e9pid\u00e9miologique (Coll int CNRS\/INSERM, 1984) IMEEE, Montpellier: 356\u2013395."},{"key":"ref10","article-title":"A new focus of cutaneous leishmaniasis caused by Leishmania infantum in northern Morocco","volume":"4","author":"M Lemrani","year":"1999","journal-title":"Giornale Italiano di Medicina Tropicale"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","DOI":"10.4081\/mr.2012.e7","article-title":"Socio-economic and environmental factors associated with Montenegro skin test positivity in an endemic area of visceral leishmaniasis in northern Morocco","volume":"3","author":"S Hamdi","year":"2012","journal-title":"Microbiology Research"},{"key":"ref12","first-page":"179","article-title":"Leishmaniose cutan\u00e9e humaine \u00e0 Leishmania infantum MON-24 au Maroc","author":"JA Rioux","year":"1996","journal-title":"Bull Soc Fr Parasitol"},{"key":"ref13","article-title":"Distribution and bionomic of sand flies in five ecologically different cutaneous leishmaniasis foci in Morocco","volume":"2013","author":"C Faraj","year":"2012","journal-title":"ISRN Epidemiology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"86","DOI":"10.4103\/0972-9062.134785","article-title":"Phlebotomus sergenti a common vector of Leishmania tropica and Toscana virus in Morocco","volume":"51","author":"N Es-Sette","year":"2014","journal-title":"Journal of vector borne diseases"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.actatropica.2017.05.016","article-title":"New epidemiological pattern of cutaneous leishmaniasis in two pre-Saharan arid provinces, southern Morocco","volume":"173","author":"MA Kbaich","year":"2017","journal-title":"Acta Tropica"},{"key":"ref16","unstructured":"Dereure J, Velez ID, Pratlong F, Denial M, Lardi M, et al. (1986) La leishmaniose visc\u00e9rale autochtone au Maroc m\u00e9ridional: Pr\u00e9sence de Leishmania infantum MON-1 chez le Chien en zone pr\u00e9saharienne. Leishmania Taxonomie et phylogen\u00e8se Application \u00e9co-\u00e9pid\u00e9miologique (Coll int CNRS\/INSERM, 1984) IMEEE, Montpellier: 421\u2013425."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1186\/s13071-016-1910-x","article-title":"New epidemiological aspects of visceral and cutaneous leishmaniasis in Taza, Morocco","volume":"9","author":"M Hakkour","year":"2016","journal-title":"Parasit Vectors"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/S0035-9203(97)90511-3","article-title":"Cutaneous leishmaniasis: an emerging epidemic focus of Leishmania tropica in north Morocco","volume":"91","author":"N Guessous-Idrissi","year":"1997","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref19","unstructured":"Plan MRHCd (2011) Monographie de la r\u00e9gion Taza-Al Hoceima-Taounate."},{"key":"ref20","first-page":"1","article-title":"Les phl\u00e9botomes de la r\u00e9gion \u00e9thiopienne (Diptera, Psychodidae)","volume":"55","author":"E Abonnenc","year":"1972","journal-title":"Cahiers de l'ORSTOM, s\u00e9rie Entomologie m\u00e9dicale et Parasitologie"},{"key":"ref21","article-title":"Lutte Contre les Leishmanioses, Guide des Activit\u00e9s","year":"2010"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.0269-283X.2004.00548.x","article-title":"Distribution of phlebotomine sandflies, of the subgenus Larroussius, in Morocco","volume":"19","author":"S Guernaoui","year":"2005","journal-title":"Medical and veterinary entomology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1093\/jmedent\/36.1.116","article-title":"Morphological and isoenzymatic differentiation of sympatric populations of Phlebotomus perniciosus and Phlebotomus longicuspis (Diptera: Psychodidae) in northern Morocco","volume":"36","author":"I Benabdennbi","year":"1999","journal-title":"Journal of medical entomology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1051\/parasite\/1983586611","article-title":"About the differentiation of females from Larroussius s. gen. 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