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Global Health Observatory data repository: Human African Trypanosomiasis 2015 [cited 2016 11th January,]. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/apps.who.int\/gho\/data\/node.main.A1635\" xlink:type=\"simple\">http:\/\/apps.who.int\/gho\/data\/node.main.A1635<\/ext-link>."},{"key":"ref4","unstructured":"WHO. London Declaration on Neglected Tropical Diseases. Ending the neglect and reaching 2020 goals. 2012 [cited 2017 04\/06]. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/unitingtocombatntds.org\/resource\/london-declaration\" xlink:type=\"simple\">http:\/\/unitingtocombatntds.org\/resource\/london-declaration<\/ext-link>."},{"issue":"1\u20132","key":"ref5","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.physbeh.2007.05.045","article-title":"Migration of African trypanosomes across the blood-brain barrier","volume":"92","author":"W Masocha","year":"2007","journal-title":"Physiology & behavior"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-308X(08)60410-8","article-title":"The treatment of human African trypanosomiasis","volume":"33","author":"J Pepin","year":"1994","journal-title":"Advances in parasitology"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.2217\/fmb.11.88","article-title":"Drug resistance in human African 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Markham","year":"2014","journal-title":"Drugs"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1128\/AAC.02580-12","article-title":"Comparative in vitro activities of GSK2251052, a novel boron-containing leucyl-tRNA synthetase inhibitor, against 916 anaerobic organisms","volume":"57","author":"EJ Goldstein","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1128\/AAC.02058-12","article-title":"Discovery of a novel class of boron-based antibacterials with activity against gram-negative bacteria","volume":"57","author":"V Hernandez","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"23","key":"ref13","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1016\/j.bmcl.2015.10.044","article-title":"Discovery of an orally bioavailable isoxazoline benzoxaborole (AN8030) as a long acting animal 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methyltransferases and of the transformation of chick embryo fibroblasts by Rous sarcoma virus","volume":"85","author":"M Vedel","year":"1978","journal-title":"Biochemical and biophysical research communications"},{"issue":"11","key":"ref37","doi-asserted-by":"crossref","first-page":"4097","DOI":"10.1128\/AAC.48.11.4097-4102.2004","article-title":"Modified fixed-ratio isobologram method for studying in vitro interactions between atovaquone and proguanil or dihydroartemisinin against drug-resistant strains of Plasmodium falciparum","volume":"48","author":"QL Fivelman","year":"2004","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"6","key":"ref38","doi-asserted-by":"crossref","first-page":"2768","DOI":"10.1128\/AAC.00044-13","article-title":"Metabolomics guides rational development of a simplified cell culture medium for drug screening against Trypanosoma brucei","volume":"57","author":"DJ Creek","year":"2013","journal-title":"Antimicrobial agents and 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Lamont","year":"1986","journal-title":"Parasitology"},{"issue":"3","key":"ref42","doi-asserted-by":"crossref","first-page":"e1003260","DOI":"10.1371\/journal.ppat.1003260","article-title":"DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes","volume":"9","author":"L Glover","year":"2013","journal-title":"PLoS Pathog"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"e1000731","DOI":"10.1371\/journal.ppat.1000731","article-title":"Persistent ER stress induces the spliced leader RNA silencing pathway (SLS), leading to programmed cell death in Trypanosoma brucei","volume":"6","author":"H Goldshmidt","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/1756-3305-5-107","article-title":"Spliced leader RNA silencing (SLS)\u2014a programmed cell death pathway in Trypanosoma brucei that is induced upon ER 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cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication","volume":"36","author":"R Brun","year":"1979","journal-title":"Acta tropica"},{"issue":"3","key":"ref59","doi-asserted-by":"crossref","first-page":"360","DOI":"10.2307\/3279142","article-title":"A simple monophasic medium for axenic culture of hemoflagellates","volume":"62","author":"RL Berens","year":"1976","journal-title":"The Journal of parasitology"},{"issue":"2","key":"ref60","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0001-706X(97)00079-X","article-title":"The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro","volume":"68","author":"B Raz","year":"1997","journal-title":"Acta tropica"},{"issue":"5","key":"ref61","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1093\/jac\/11.5.427","article-title":"The fractional inhibitory concentration (FIC) index as a measure of synergy","volume":"11","author":"MJ Hall","year":"1983","journal-title":"The Journal of antimicrobial chemotherapy"},{"issue":"Pt 1","key":"ref62","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1242\/jcs.95.1.49","article-title":"Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei","volume":"95","author":"R Woodward","year":"1990","journal-title":"Journal of cell science"},{"issue":"6","key":"ref63","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.mib.2003.10.010","article-title":"Coordination of cell cycle and cytokinesis in Trypanosoma brucei","volume":"6","author":"PG McKean","year":"2003","journal-title":"Current opinion in microbiology"},{"issue":"7","key":"ref64","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nature methods"},{"key":"ref65","unstructured":"Team RC. R: A language and environment for statistical computing 2017 [cited 2017 04\/06]. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.R-project.org\/\" xlink:type=\"simple\">https:\/\/www.R-project.org\/<\/ext-link>."},{"issue":"10","key":"ref66","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1038\/nbt.2377","article-title":"A cross-platform toolkit for mass spectrometry and proteomics","volume":"30","author":"MC Chambers","year":"2012","journal-title":"Nature biotechnology"},{"issue":"7","key":"ref67","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1093\/bioinformatics\/bts069","article-title":"IDEOM: an Excel interface for analysis of LC-MS-based metabolomics data","volume":"28","author":"DJ Creek","year":"2012","journal-title":"Bioinformatics"},{"issue":"7","key":"ref68","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1021\/ac2000994","article-title":"PeakML\/mzMatch: a file format, Java library, R library, and tool-chain for mass spectrometry data analysis","volume":"83","author":"RA Scheltema","year":"2011","journal-title":"Analytical chemistry"},{"issue":"2","key":"ref69","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1093\/bioinformatics\/bts674","article-title":"mzMatch-ISO: an R tool for the annotation and relative quantification of isotope-labelled mass spectrometry data","volume":"29","author":"A Chokkathukalam","year":"2013","journal-title":"Bioinformatics"},{"issue":"D1","key":"ref70","doi-asserted-by":"crossref","first-page":"D471","DOI":"10.1093\/nar\/gkv1164","article-title":"The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway\/genome databases","volume":"44","author":"R Caspi","year":"2016","journal-title":"Nucleic acids research"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"W423","DOI":"10.1093\/nar\/gkn282","article-title":"KEGG Atlas mapping for global analysis of metabolic pathways","volume":"36","author":"S Okuda","year":"2008","journal-title":"Nucleic acids research"},{"issue":"W1","key":"ref72","doi-asserted-by":"crossref","first-page":"W251","DOI":"10.1093\/nar\/gkv380","article-title":"MetaboAnalyst 3.0\u2014making metabolomics more meaningful","volume":"43","author":"J Xia","year":"2015","journal-title":"Nucleic acids research"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0006450","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T00:00:00Z","timestamp":1527638400000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0006450","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T17:31:44Z","timestamp":1661275904000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0006450"}},"subtitle":[],"editor":[{"given":"Timothy G.","family":"Geary","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,5,14]]},"references-count":72,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2018,5,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0006450","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0006450","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,14]]}}}