{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:56:59Z","timestamp":1787021819555,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pntd.0006550","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T00:00:00Z","timestamp":1529366400000}}],"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI113475"],"award-info":[{"award-number":["R21AI113475"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0006550","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T13:51:16Z","timestamp":1527861076000},"page":"e0006550","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy"],"prefix":"10.1371","volume":"12","author":[{"given":"Alejandro J.","family":"Krolewiecki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artemis","family":"Koukounari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miryam","family":"Romano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reynaldo N.","family":"Caro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan L.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Fleitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Cimino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0208-635X","authenticated-orcid":true,"given":"Clive J.","family":"Shiff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,6,1]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"e2165","DOI":"10.1371\/journal.pntd.0002165","article-title":"A public health response against Strongyloides stercoralis: time to look at soil-transmitted helminthiasis in full","volume":"7","author":"AJ Krolewiecki","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref2","unstructured":"WHO. Soil-transmitted helminthiases: eliminating soil-transmitted helminthiases as a public health problem in children: progress report 2001\u20132010 and strategic plan 2011\u20132020. 2012. p. 89."},{"key":"ref3","unstructured":"WHO. Preventive chemotherapy to contrl soil-transmitted helminth infectons in at-risk populations groups. Geneva: 2017."},{"key":"ref4","unstructured":"WHO. Helminth control in school age children: a guide for managers of control programmes. 2nd ed. Geneva: WHO; 2011. p. 90."},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"e2002","DOI":"10.1371\/journal.pntd.0002002","article-title":"The laboratory diagnosis and follow up of strongyloidiasis: a systematic review","volume":"7","author":"A Requena-Mendez","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"e2214","DOI":"10.1371\/journal.pntd.0002214","article-title":"<italic>Strongyloides stercorali<\/italic>s: A Plea for Action","volume":"7","author":"Z Bisoffi","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"e2640","DOI":"10.1371\/journal.pntd.0002640","article-title":"Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection","volume":"8","author":"Z Bisoffi","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"e0004578","DOI":"10.1371\/journal.pntd.0004578","article-title":"Improved PCR-Based Detection of Soil Transmitted Helminth Infections Using a Next-Generation Sequencing Approach to Assay Design","volume":"10","author":"N Pilotte","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1186\/s13071-015-0994-z","article-title":"Identification of human intestinal parasites affecting an asymptomatic peri-urban Argentinian population using multi-parallel quantitative real-time polymerase chain reaction","volume":"8","author":"RO Cimino","year":"2015","journal-title":"Parasit Vectors"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"24","DOI":"10.11613\/BM.2012.004","article-title":"Free DNA\u2014new potential analyte in clinical laboratory diagnostics?","volume":"22","author":"J Wagner","year":"2012","journal-title":"Biochemia medica"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.pt.2016.01.006","article-title":"Cell-Free DNA as a Diagnostic Tool for Human Parasitic Infections","volume":"32","author":"KG Weerakoon","year":"2016","journal-title":"Trends Parasitol"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1309\/LMKKOX6UJZQGW0EA","article-title":"Recent advances in clinical applications of circulating cell-free DNA integrity","volume":"45","author":"J Yu","year":"2014","journal-title":"Laboratory medicine"},{"issue":"8","key":"ref13","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1093\/clinchem\/46.8.1078","article-title":"Genetic analysis of DNA excreted in urine: a new approach for detecting specific genomic DNA sequences from cells dying in an organism","volume":"46","author":"I Botezatu","year":"2000","journal-title":"Clinical chemistry"},{"issue":"6","key":"ref14","doi-asserted-by":"crossref","first-page":"998","DOI":"10.4269\/ajtmh.2011.10-0691","article-title":"Diagnosis of Schistosoma haematobium by detection of specific DNA fragments from filtered urine samples","volume":"84","author":"OA Ibironke","year":"2011","journal-title":"Am J Trop Med Hyg"},{"issue":"7","key":"ref15","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.1158\/1078-0432.CCR-04-2147","article-title":"Increased mitochondrial DNA content in saliva associated with head and neck cancer","volume":"11","author":"WW Jiang","year":"2005","journal-title":"Clin Cancer Res"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1053\/j.gastro.2008.05.039","article-title":"Analysis of mutations in DNA isolated from plasma and stool of colorectal cancer patients","volume":"135","author":"F Diehl","year":"2008","journal-title":"Gastroenterology"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.lungcan.2008.01.007","article-title":"Can free DNA be detected in sputum of lung cancer patients?","volume":"61","author":"MA van der Drift","year":"2008","journal-title":"Lung cancer"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S1525-1578(10)60497-7","article-title":"Human urine contains small, 150 to 250 nucleotide-sized, soluble DNA derived from the circulation and may be useful in the detection of colorectal cancer","volume":"6","author":"YH Su","year":"2004","journal-title":"The Journal of molecular diagnostics: JMD"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"e91144","DOI":"10.1371\/journal.pone.0091144","article-title":"Detection of parasite-specific DNA in urine sediment obtained by filtration differentiates between single and mixed infections of Schistosoma mansoni and S. haematobium from endemic areas in Ghana","volume":"9","author":"N Lodh","year":"2014","journal-title":"PLoS One"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.prevetmed.2017.01.006","article-title":"STARD-BLCM: Standards for the Reporting of Diagnostic accuracy studies that use Bayesian Latent Class Models","volume":"138","author":"P NS Kostoulas","year":"2017","journal-title":"Prev Vet Med"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1207\/S15328007SEM0904_8","article-title":"How to Use a Monte Carlo Study to Decide on Sample Size and Determine Power","volume":"9","author":"B.O. Muthen LKaM","year":"2002","journal-title":"Structural Equation Modelling: A Multidisiplinary Journal"},{"key":"ref22","article-title":"Mplus User&apos;s Guide","author":"LK Muthen","year":"2010"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.actatropica.2016.07.014","article-title":"Diagnosis of Strongyloides stercoralis: Detection of parasite-derived DNA in urine","volume":"163","author":"N Lodh","year":"2016","journal-title":"Acta tropica"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"e955","DOI":"10.1371\/journal.pntd.0000955","article-title":"A coproantigen diagnostic test for Strongyloides infection","volume":"5","author":"AM Sykes","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1128\/9781555817961.ch11","article-title":"Diagnostic Medical Parasitology","author":"L Garcia","year":"2001"},{"issue":"10","key":"ref26","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1128\/CVI.00259-10","article-title":"Improved diagnosis of Strongyloides stercoralis using recombinant antigen-based serologies in a community-wide study in northern Argentina","volume":"17","author":"AJ Krolewiecki","year":"2010","journal-title":"Clinical and vaccine immunology: CVI"},{"issue":"1\u20132","key":"ref27","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0166-6851(02)00214-1","article-title":"Characterization of a recombinant immunodiagnostic antigen (NIE) from Strongyloides stercoralis L3-stage larvae","volume":"125","author":"V Ravi","year":"2002","journal-title":"Mol Biochem Parasitol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.actatropica.2016.07.014","article-title":"Diagnosis of <italic>Strongyloides stercoralis<\/italic>: Detection of parasite-derived DNA in urine","volume":"163","author":"N Lodh","year":"2016","journal-title":"Acta Tropica"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/oxfordjournals.aje.a117428","article-title":"Bayesian estimation of disease prevalence and the parameters of diagnostic tests in the absence of a gold standard","volume":"141","author":"L Joseph","year":"1995","journal-title":"Am J Epidemiol"},{"issue":"7","key":"ref30","doi-asserted-by":"crossref","first-page":"e1254","DOI":"10.1371\/journal.pntd.0001254","article-title":"Randomized clinical trial on ivermectin versus thiabendazole for the treatment of strongyloidiasis","volume":"5","author":"Z Bisoffi","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"e0003491","DOI":"10.1371\/journal.pntd.0003491","article-title":"Accuracy of five serologic tests for the follow up of Strongyloides stercoralis infection","volume":"9","author":"D Buonfrate","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1023\/A:1008929526011","article-title":"WinBUGS\u2014A Bayesian Modelling Framework: concepts, structure and extensibility","volume":"10","author":"D Lunn","year":"2000","journal-title":"Statistics nas Computing"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1177\/001316446002000104","article-title":"A coefficient of agreement fro nomincal scales","volume":"20","author":"J Cohen","year":"1960","journal-title":"Educational and Psychological Measurement"},{"key":"ref34","article-title":"The London Declaration on Neglected Tropical Diseases: 5 years on","author":"DH Molyneux","year":"2017","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s13071-017-1979-x","article-title":"Assessing the interruption of the transmission of human helminths with mass drug administration alone: optimizing the design of cluster randomized trials","volume":"10","author":"R Anderson","year":"2017","journal-title":"Parasit Vectors"},{"issue":"7","key":"ref36","doi-asserted-by":"crossref","first-page":"e3059","DOI":"10.1371\/journal.pntd.0003059","article-title":"Predicted impact of mass drug administration on the development of protective immunity against Schistosoma haematobium","volume":"8","author":"KM Mitchell","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"ref37","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.ijpara.2014.05.009","article-title":"Sensitivity of diagnostic tests for human soil-transmitted helminth infections: a meta-analysis in the absence of a true gold standard","volume":"44","author":"B Nikolay","year":"2014","journal-title":"Int J Parasitol"},{"issue":"3","key":"ref38","doi-asserted-by":"crossref","first-page":"508","DOI":"10.4269\/ajtmh.16-0266","article-title":"Molecular Diagnostics for Soil-Transmitted Helminths","volume":"95","author":"EM O'Connell","year":"2016","journal-title":"Am J Trop Med Hyg"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.ijpara.2009.09.003","article-title":"Estimating the sensitivity and specificity of Kato-Katz stool examination technique for detection of hookworms, Ascaris lumbricoides and Trichuris trichiura infections in humans in the absence of a &apos;gold standard&apos;","volume":"40","author":"MR Tarafder","year":"2010","journal-title":"Int J Parasitol"},{"issue":"4","key":"ref40","doi-asserted-by":"crossref","first-page":"FSO295","DOI":"10.4155\/fsoa-2017-0140","article-title":"Kinetics of circulating cell-free DNA for biomedical applications: critical appraisal of the literature","volume":"4","author":"S Khier","year":"2018","journal-title":"Future science OA"},{"issue":"1","key":"ref41","doi-asserted-by":"crossref","first-page":"e1464","DOI":"10.1371\/journal.pntd.0001464","article-title":"Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis","volume":"6","author":"O Ibironke","year":"2012","journal-title":"PLoS Negl Trop Dis"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0006550","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T00:00:00Z","timestamp":1529366400000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0006550","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T20:26:03Z","timestamp":1604348763000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0006550"}},"subtitle":[],"editor":[{"given":"Sitara S. R.","family":"Ajjampur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,6,1]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2018,6,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0006550","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,1]]}}}