{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T04:19:36Z","timestamp":1776140376505,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0006683","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000}}],"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2018,8,2]],"date-time":"2018-08-02T00:00:00Z","timestamp":1533168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0006683","type":"journal-article","created":{"date-parts":[[2018,8,2]],"date-time":"2018-08-02T18:12:42Z","timestamp":1533233562000},"page":"e0006683","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide"],"prefix":"10.1371","volume":"12","author":[{"given":"Na","family":"Cheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9828-3077","authenticated-orcid":true,"given":"Xue-Nian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Ting","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Fang","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,8,2]]},"reference":[{"issue":"10020","key":"ref1","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/S0140-6736(15)60313-0","article-title":"Clonorchiasis","volume":"387","author":"MB Qian","year":"2016","journal-title":"Lancet (London, England)"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/2049-9957-1-4","article-title":"The global epidemiology of clonorchiasis and its relation with cholangiocarcinoma","volume":"1","author":"MB Qian","year":"2012","journal-title":"Infectious diseases of poverty"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S1473-3099(04)01252-6","article-title":"Clonorchiasis: a key foodborne zoonosis","volume":"5","author":"ZR Lun","year":"2005","journal-title":"China. Lancet Infect Dis"},{"issue":"5","key":"ref4","first-page":"332","article-title":"A national survey on current status of the important parasitic diseases in human population","volume":"23","year":"2005","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S1470-2045(09)70096-8","article-title":"A review of human carcinogens\u2014Part B: biological agents","volume":"10","author":"V Bouvard","year":"2009","journal-title":"The Lancet Oncology"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.3201\/eid1110.050614","article-title":"Emerging foodborne trematodiasis","volume":"11","author":"J Keiser","year":"2005","journal-title":"Emerg Infect Dis"},{"issue":"7","key":"ref7","doi-asserted-by":"crossref","first-page":"e201","DOI":"10.1371\/journal.pmed.0040201","article-title":"Liver fluke induces cholangiocarcinoma","volume":"4","author":"B Sripa","year":"2007","journal-title":"PLos Med"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3347\/kjp.2003.41.1.35","article-title":"Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA","volume":"41","author":"MH Choi","year":"2003","journal-title":"Korean J Parasitol"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.3346\/jkms.2010.25.9.1272","article-title":"Seroprevalence of tissue invading parasitic infections diagnosed by ELISA in Korea","volume":"25","author":"MK Lee","year":"2010","journal-title":"Journal of Korean medical science"},{"issue":"7","key":"ref10","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.1128\/JCM.02506-09","article-title":"Performance of an enzyme-linked immunosorbent assay for detection of Clonorchis sinensis infestation in high- and low-risk groups","volume":"48","author":"YJ Kim","year":"2010","journal-title":"Journal of clinical microbiology"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1128\/CDLI.11.4.814-817.2004","article-title":"Evaluation of Clonorchis sinensis recombinant 7-kilodalton antigen for serodiagnosis of clonorchiasis","volume":"11","author":"QP Zhao","year":"2004","journal-title":"Clinical and diagnostic laboratory immunology"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.exppara.2007.04.003","article-title":"Clonorchis sinensis: expression, characterization, immunolocalization and serological reactivity of one excretory\/secretory antigen-LPAP homologue","volume":"117","author":"F Hu","year":"2007","journal-title":"Experimental parasitology"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1007\/s00436-007-0676-7","article-title":"Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1alpha","volume":"101","author":"TY Kim","year":"2007","journal-title":"Parasitology research"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1128\/CDLI.11.2.411-416.2004","article-title":"Molecular expression of a cysteine proteinase of Clonorchis sinensis and its application to an enzyme-linked immunosorbent assay for immunodiagnosis of clonorchiasis","volume":"11","author":"I Nagano","year":"2004","journal-title":"Clinical and diagnostic laboratory immunology"},{"issue":"3\u20134","key":"ref15","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0014-4894(03)00056-0","article-title":"Clonorchis sinensis: immunolocalization of 26 kDa glutathione S-transferase in adult worms","volume":"102","author":"SJ Hong","year":"2002","journal-title":"Experimental parasitology"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1006\/expr.2001.4606","article-title":"Clonorchis sinensis: molecular cloning and characterization of 28-kDa glutathione S-transferase","volume":"97","author":"SY Kang","year":"2001","journal-title":"Experimental parasitology"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0166-6851(00)00220-6","article-title":"Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis","volume":"108","author":"SJ Hong","year":"2000","journal-title":"Mol Biochem Parasitol"},{"issue":"4","key":"ref18","first-page":"245","article-title":"Molecular cloning and characterization of a novel Clonorchis sinensis antigenic protein containing tandem repeat sequences","volume":"31","author":"Q Liu","year":"2013","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"issue":"6","key":"ref19","first-page":"401","article-title":"Cloning, expression of PPMP antigen genes of Clonorchis sinensis and immunogenic identification of the recombinant proteins","volume":"28","author":"XN Xu","year":"2010","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"issue":"3","key":"ref20","first-page":"172","article-title":"Evaluation of Clonorchis sinensis PPMP I antigen Cs2 recombinant protein for immunodiagnosis of clonorchiasis","volume":"29","author":"Y Zhou","year":"2011","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"issue":"3","key":"ref21","first-page":"135","article-title":"Immunological characterization and localization of Cs4 recombinant protein of Clonorchis sinensis, one of the glycine rich antigen 2a gene family member","volume":"38","author":"Z CNXXZHZYTYBYZLXBJHLXF","year":"2011","journal-title":"Guo Ji Yi Xue Ji Sheng Chong Bing Za Zhi"},{"issue":"7","key":"ref22","doi-asserted-by":"crossref","first-page":"3939","DOI":"10.1128\/IAI.00101-06","article-title":"Cloning, characterization, and serodiagnostic evaluation of Leishmania infantum tandem repeat proteins","volume":"74","author":"Y Goto","year":"2006","journal-title":"Infection and immunity"},{"key":"ref23","first-page":"123","article-title":"Murine monoclonal antibody development","volume":"51","author":"DR Johnson","year":"1995","journal-title":"Methods in molecular biology (Clifton, NJ)"},{"issue":"4","key":"ref24","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.4049\/jimmunol.135.4.2589","article-title":"Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies","volume":"135","author":"B Akerstrom","year":"1985","journal-title":"Journal of immunology (Baltimore, Md: 1950)"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.bbrc.2011.01.048","article-title":"Biased cellular locations of tandem repeat antigens in African trypanosomes","volume":"405","author":"Y Goto","year":"2011","journal-title":"Biochemical and biophysical research communications"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1073\/pnas.82.2.543","article-title":"Interspersed blocks of repetitive and charged amino acids in a dominant immunogen of Plasmodium falciparum","volume":"82","author":"HD Stahl","year":"1985","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"8","key":"ref27","doi-asserted-by":"crossref","first-page":"e1003488","DOI":"10.1371\/journal.ppat.1003488","article-title":"Asparagine repeats in Plasmodium falciparum proteins: good for nothing?","volume":"9","author":"V Muralidharan","year":"2013","journal-title":"PLoS Pathog"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1128\/IAI.01205-06","article-title":"Bioinformatic identification of tandem repeat antigens of the Leishmania donovani complex","volume":"75","author":"Y Goto","year":"2007","journal-title":"Infection and immunity"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1073\/pnas.90.2.775","article-title":"Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis","volume":"90","author":"JM Burns Jr.","year":"1993","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1128\/IAI.01102-09","article-title":"Upregulated expression of B-cell antigen family tandem repeat proteins by Leishmania amastigotes","volume":"78","author":"Y Goto","year":"2010","journal-title":"Infection and immunity"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1086\/514199","article-title":"The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain","volume":"177","author":"CA Buscaglia","year":"1998","journal-title":"J Infect Dis"},{"issue":"9","key":"ref32","doi-asserted-by":"crossref","first-page":"3967","DOI":"10.1128\/IAI.00604-08","article-title":"Immunological dominance of Trypanosoma cruzi tandem repeat proteins","volume":"76","author":"Y Goto","year":"2008","journal-title":"Infection and immunity"},{"issue":"Pt 2","key":"ref33","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1017\/S0031182099006253","article-title":"The major tegumental antigen of Fasciola hepatica contains repeated elements","volume":"121","author":"A Trudgett","year":"2000","journal-title":"Parasitology"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0166-6851(00)00298-X","article-title":"Identification of a Clonorchis sinensis gene encoding a vitellaria antigenic protein containing repetitive sequences","volume":"111","author":"HJ Yang","year":"2000","journal-title":"Mol Biochem Parasitol"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3347\/kjp.2001.39.1.57","article-title":"Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis","volume":"39","author":"TY Kim","year":"2001","journal-title":"Korean J Parasitol"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3347\/kjp.1998.36.3.183","article-title":"Immunodiagnosis of clonorchiasis using a recombinant antigen","volume":"36","author":"TS Yong","year":"1998","journal-title":"Korean J Parasitol"},{"issue":"1\u20132","key":"ref37","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.molimm.2012.06.001","article-title":"Structural analysis of B-cell epitopes in antibody:protein complexes","volume":"53","author":"JV Kringelum","year":"2013","journal-title":"Molecular immunology"},{"issue":"Suppl 1","key":"ref38","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1186\/1472-6807-10-S1-S6","article-title":"Mining for the antibody-antigen interacting associations that predict the B cell epitopes","volume":"10","author":"L Zhao","year":"2010","journal-title":"BMC structural biology"},{"issue":"5","key":"ref39","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1002\/cbdv.201100360","article-title":"Predicting antigenicity of proteins in a bacterial proteome; a protein microarray and naive Bayes classification approach","volume":"9","author":"L Liang","year":"2012","journal-title":"Chemistry & biodiversity"},{"issue":"12","key":"ref40","doi-asserted-by":"crossref","first-page":"e50748","DOI":"10.1371\/journal.pone.0050748","article-title":"Diagnostic peptide discovery: prioritization of pathogen diagnostic markers using multiple features","volume":"7","author":"SJ Carmona","year":"2012","journal-title":"PloS one"},{"issue":"8","key":"ref41","doi-asserted-by":"crossref","first-page":"e0134835","DOI":"10.1371\/journal.pone.0134835","article-title":"Analysis of Conformational B-Cell Epitopes in the Antibody-Antigen Complex Using the Depth Function and the Convex Hull","volume":"10","author":"W Zheng","year":"2015","journal-title":"PloS one"},{"issue":"51","key":"ref42","doi-asserted-by":"crossref","first-page":"48009","DOI":"10.1074\/jbc.M108941200","article-title":"The cGMP-gated channel and related glutamic acid-rich proteins interact with peripherin-2 at the rim region of rod photoreceptor disc membranes","volume":"276","author":"A Poetsch","year":"2001","journal-title":"The Journal of biological chemistry"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"13370","DOI":"10.1038\/srep13370","article-title":"An equation to estimate the difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic peptide","volume":"5","author":"Y Guan","year":"2015","journal-title":"Scientific reports"},{"issue":"6746","key":"ref44","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1038\/23468","article-title":"Interaction of glutamic-acid-rich proteins with the cGMP signalling pathway in rod photoreceptors","volume":"400","author":"HG Korschen","year":"1999","journal-title":"Nature"},{"issue":"30","key":"ref45","doi-asserted-by":"crossref","first-page":"18083","DOI":"10.1016\/S0021-9258(19)84681-1","article-title":"Molecular cloning of a histidine-rich Ca2+-binding protein of sarcoplasmic reticulum that contains highly conserved repeated elements","volume":"264","author":"SL Hofmann","year":"1989","journal-title":"The Journal of biological chemistry"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0006683","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0006683","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T04:01:26Z","timestamp":1693800086000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0006683"}},"subtitle":[],"editor":[{"given":"Maria Elena","family":"Bottazzi","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,8,2]]},"references-count":45,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2018,8,2]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0006683","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0006683","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,2]]}}}