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Vectors"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/physci241020a0","article-title":"Controlled nucleation for regulation of particle-size in monodisperse gold suspensions","volume":"241","author":"G Frens","year":"1973","journal-title":"Nat Phys Science"},{"key":"ref37","unstructured":"Delgado JM, Soriano F, Camacho A, Rojas J, Mendoza J, Rojas A. Evaluation of a new immunochromatographic assay for the detection of antibodies against Echinococcus granulosus. 2010. XX ECCMID. Vienna, Austria. P2125 (<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.blackwellpublishing.com\/eccmid20\/PDFs\/eccmid20_posters.pdf\" xlink:type=\"simple\">http:\/\/www.blackwellpublishing.com\/eccmid20\/PDFs\/eccmid20_posters.pdf<\/ext-link>)."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e0004585","DOI":"10.1371\/journal.pntd.0004585","article-title":"Diagnostic Accuracy of Antigen 5-Based ELISAs for Human Cystic Echinococcosis","volume":"10","author":"D Pagnozzi","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1365-3024.2010.01264.x","article-title":"Favourable prognostic value of antibodies anti-HSP20 in patients with cystic echinococcosis: a differential immunoproteomic approach","volume":"33","author":"D Vacirca","year":"2011","journal-title":"Parasite Immunol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1007\/s11033-010-0332-7","article-title":"Molecular characterization of antigen B2 subunit in two genotypes of <italic>Echinococcus granulosus<\/italic> from Indian bubaline isolates, its stage specific expression and serological evaluation","volume":"38","author":"D Pan","year":"2011","journal-title":"Mol Biol Rep"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1251\/bpo74","article-title":"Recombinant antigens for immunodiagnosis of cystic echinococcosis","volume":"6","author":"J Li","year":"2004","journal-title":"Biol Proced Online"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.trstmh.2008.09.020","article-title":"Isolation and molecular characterization of recombinant <italic>Echinococcus granulosus<\/italic> P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients","volume":"103","author":"N Ben Nouir","year":"2009","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/tmi.12859","article-title":"Follow-up of surgically treated patients with cystic echinococcosis: can novel recombinant antigens compete with imaging? 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