{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:45:15Z","timestamp":1770882315241,"version":"3.50.1"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"crossref","award":["RSA5580003"],"award-info":[{"award-number":["RSA5580003"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["089275\/Z\/09\/Z"],"award-info":[{"award-number":["089275\/Z\/09\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0007232","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T18:41:19Z","timestamp":1554921679000},"page":"e0007232","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Whole cell matrix assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8164-3523","authenticated-orcid":true,"given":"Piengchan","family":"Sonthayanon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janthima","family":"Jaresitthikunchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0989-6857","authenticated-orcid":true,"given":"Suthee","family":"Mangmee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tipparat","family":"Thiangtrongjit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Premjit","family":"Amornchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Newton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rattanaphone","family":"Phetsouvanh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas PJ","family":"Day","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sittiruk","family":"Roytrakul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,4,10]]},"reference":[{"issue":"9","key":"ref1","doi-asserted-by":"crossref","first-page":"e0003898","DOI":"10.1371\/journal.pntd.0003898","article-title":"Global Morbidity and Mortality of Leptospirosis: A Systematic Review","volume":"9","author":"F Costa","year":"2015","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"ref2","first-page":"60","article-title":"Annual Epidemiological Surveillance Report 2011","author":"S Hinjoy","year":"2011"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3201\/eid1301.060718","article-title":"Clinical diagnosis and geographic distribution of leptospirosis, Thailand","volume":"13","author":"V Wuthiekanun","year":"2007","journal-title":"Emerg Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1471-2334-2-13","article-title":"A quantitative PCR (TaqMan) assay for pathogenic <italic>Leptospira<\/italic> spp","volume":"2","author":"LD Smythe","year":"2002","journal-title":"BMC Infect Dis"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"471","DOI":"10.4269\/ajtmh.2011.11-0276","article-title":"Leptospirosis outbreak in Sri Lanka in 2008: lessons for assessing the global burden of disease","volume":"85","author":"SB Agampodi","year":"2011","journal-title":"Am J Trop Med Hyg"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"614","DOI":"10.4269\/ajtmh.2011.10-0473","article-title":"Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand","volume":"84","author":"P Sonthayanon","year":"2011","journal-title":"Am J Trop Med Hyg"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"9213","DOI":"10.1038\/s41598-018-27555-2","article-title":"Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of <italic>Leptospira<\/italic> at the Onset of Disease","volume":"8","author":"LM Esteves","year":"2018","journal-title":"Scientific reports"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1186\/1756-0500-7-330","article-title":"<italic>Leptospira<\/italic> species and serovars identified by MALDI-TOF mass spectrometry after database implementation","volume":"7","author":"A Calderaro","year":"2014","journal-title":"BMC research notes"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/1471-2180-12-185","article-title":"<italic>Leptospira<\/italic> spp. strain identification by MALDI TOF MS is an equivalent tool to 16S rRNA gene sequencing and multi locus sequence typing (MLST)","volume":"12","author":"A Rettinger","year":"2012","journal-title":"BMC microbiology"},{"issue":"3\u20134","key":"ref10","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.vetmic.2012.04.013","article-title":"Rapid MALDI-TOF mass spectrometry identification of <italic>Leptospira<\/italic> organisms: a reply","volume":"159","author":"Z Djelouadji","year":"2012","journal-title":"Vet Microbiol"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1128\/JCM.02430-06","article-title":"Optimization of culture of <italic>Leptospira<\/italic> from humans with leptospirosis","volume":"45","author":"V Wuthiekanun","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1186\/1471-2334-11-338","article-title":"Molecular detection and speciation of pathogenic <italic>Leptospira<\/italic> spp. in blood from patients with culture-negative leptospirosis","volume":"11","author":"S Boonsilp","year":"2011","journal-title":"BMC Infect Dis"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"e56","DOI":"10.1371\/journal.pntd.0000056","article-title":"A dominant clone of <italic>Leptospira interrogans<\/italic> associated with an outbreak of human leptospirosis in Thailand","volume":"1","author":"J Thaipadungpanit","year":"2007","journal-title":"PLoS neglected tropical diseases"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1128\/AAC.01812-12","article-title":"Rapid isolation and susceptibility testing of <italic>Leptospira<\/italic> spp. using a new solid medium, LVW agar","volume":"57","author":"V Wuthiekanun","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"4","key":"ref15","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.jasms.2004.12.018","article-title":"Optimization of a MALDI TOF-TOF mass spectrometer for intact protein analysis","volume":"16","author":"Z Liu","year":"2005","journal-title":"J Am Soc Mass Spectrom"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4269\/ajtmh.15-0180","article-title":"Antimicrobial Disk Susceptibility Testing of <italic>Leptospira<\/italic> spp. Using Leptospira Vanaporn Wuthiekanun (LVW) Agar","volume":"93","author":"V Wuthiekanun","year":"2015","journal-title":"Am J Trop Med Hyg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13104-017-2384-4","article-title":"Meta-analysis to estimate the load of <italic>Leptospira<\/italic> excreted in urine: beyond rats as important sources of transmission in low-income rural communities","volume":"10","author":"V Barragan","year":"2017","journal-title":"BMC research notes"},{"issue":"6","key":"ref18","doi-asserted-by":"crossref","first-page":"e2902","DOI":"10.1371\/journal.pntd.0002902","article-title":"Epidemiology of <italic>Leptospira<\/italic> transmitted by rodents in southeast Asia","volume":"8","author":"JF Cosson","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"ref19","first-page":"375","article-title":"Lipopolysaccharide biosynthesis in <italic>Leptospira<\/italic>","volume":"2","author":"DM Bulach","year":"2000","journal-title":"J Mol Microbiol Biotechnol"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"695","DOI":"10.4269\/ajtmh.2009.09-0252","article-title":"The microscopic agglutination test (MAT) is an unreliable predictor of infecting <italic>Leptospira<\/italic> serovar in Thailand","volume":"81","author":"LD Smythe","year":"2009","journal-title":"Am J Trop Med Hyg"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0007232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T15:50:50Z","timestamp":1589212250000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007232"}},"subtitle":[],"editor":[{"given":"Tao","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,10]]},"references-count":20,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0007232","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,10]]}}}