{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T22:54:47Z","timestamp":1771455287486,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0007363","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000}}],"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0007363","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T13:24:55Z","timestamp":1560777895000},"page":"e0007363","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Development and validation of a multiplexed-tandem qPCR tool for diagnostics of human soil-transmitted helminth infections"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1291-6783","authenticated-orcid":true,"given":"Katharina","family":"Stracke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille V.","family":"Awburn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Vaz Nery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virak","family":"Khieu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca J.","family":"Traub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron R.","family":"Jex","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"key":"ref1","unstructured":"World Health Organization. Soil-Transmitted Helminthiases. Eliminating Soil-Transmitted Helminthiases as a Public Health Problem in Children: Progress Report 2001\u20132010 and Strategic Plan 2011\u20132020. World Health Organization, Geneva, Switzerland. 2012."},{"issue":"10053","key":"ref2","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/S0140-6736(16)31460-X","article-title":"Global, regional, and national disability-adjusted life-years (DALYs) for 315 diseases and injuries and healthy life expectancy (HALE), 1990\u20132015: a systematic analysis for the Global Burden of Disease Study 2015","volume":"388","author":"NJ Kassebaum","year":"2016","journal-title":"The Lancet"},{"issue":"9521","key":"ref3","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1016\/S0140-6736(06)68653-4","article-title":"Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm","volume":"367","author":"J Bethony","year":"2006","journal-title":"The Lancet"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-7-37","article-title":"Global numbers of infection and disease burden of soil transmitted helminth infections in 2010","volume":"7","author":"RL Pullan","year":"2014","journal-title":"Parasites & Vectors"},{"key":"ref5","first-page":"221","article-title":"Global Epidemiology, Ecology","volume":"62","author":"S Brooker","year":"2006","journal-title":"and Control of Soil-Transmitted Helminth Infections"},{"issue":"9674","key":"ref6","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1016\/S0140-6736(09)60233-6","article-title":"Rescuing the bottom billion through control of neglected tropical diseases","volume":"373","author":"PJ Hotez","year":"2009","journal-title":"The Lancet"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/B978-0-12-385897-9.00004-5","article-title":"Soil-transmitted helminths of humans in Southeast Asia\u2014towards integrated control","volume":"74","author":"AR Jex","year":"2011","journal-title":"Adv Parasitol"},{"key":"ref8","article-title":"Helminth infections: soil-transmitted helminth infections and schistosomiasis","author":"PJ Hotez","year":"2006","journal-title":"Helminth infections: soil-transmitted helminth infections and schistosomiasis"},{"key":"ref9","article-title":"Guideline: preventive chemotherapy to control soil-transmitted helminth infections in at-risk population groups","year":"2017","journal-title":"Guideline: preventive chemotherapy to control soil-transmitted helminth infections in at-risk population groups"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13071-016-1310-2","article-title":"Epidemiological surveys of, and research on, soil-transmitted helminths in Southeast Asia: a systematic review","volume":"9","author":"JC Dunn","year":"2016","journal-title":"Parasites & vectors"},{"key":"ref11","article-title":"Helminth control in school-age children: a guide for managers of control programmes","year":"2011"},{"issue":"4","key":"ref12","first-page":"867","article-title":"The global state of helminth control and elimination in children","volume":"64","author":"JE Weatherhead","year":"2017","journal-title":"Pediatric Clinics"},{"issue":"12","key":"ref13","doi-asserted-by":"crossref","first-page":"e3313","DOI":"10.1371\/journal.pntd.0003313","article-title":"Simple fecal flotation is a superior alternative to guadruple Kato Katz smear examination for the detection of hookworm eggs in human stool","volume":"8","author":"T Inpankaew","year":"2014","journal-title":"PLoS neglected tropical diseases"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.pt.2009.01.004","article-title":"Diagnostic dilemmas in helminthology: what tools to use and when?","volume":"25","author":"R Bergquist","year":"2009","journal-title":"Trends Parasitol"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"175","DOI":"10.4103\/0974-777X.81696","article-title":"Prevention of soil-transmitted helminth infection","volume":"3","author":"L Mascarini-Serra","year":"2011","journal-title":"Journal of global infectious diseases"},{"issue":"10","key":"ref16","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1016\/j.ijpara.2010.04.004","article-title":"Estimating the global distribution and disease burden of intestinal nematode infections: adding up the numbers\u2014a review","volume":"40","author":"S Brooker","year":"2010","journal-title":"Int J Parasitol"},{"issue":"03","key":"ref17","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1017\/S0031182000078410","article-title":"The evaluation of potential global morbidity attributable to intestinal nematode infections","volume":"109","author":"M Chan","year":"1994","journal-title":"Parasitology"},{"key":"ref18","first-page":"19","article-title":"Soil-transmitted helminth infections: updating the global picture","author":"NR De Silva","year":"2003","journal-title":"Trends Parasitol"},{"key":"ref19","first-page":"i","article-title":"Prevention and control of schistosomiasis and soil-transmitted helminthiasis","volume":"912","year":"2002"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.pt.2017.01.007","article-title":"Economic considerations for moving beyond the Kato-Katz technique for diagnosing intestinal parasites as we move towards elimination","volume":"33","author":"HC Turner","year":"2017","journal-title":"Trends in parasitology"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.pt.2009.01.004","article-title":"Diagnostic dilemmas in helminthology: what tools to use and when?","volume":"25","author":"R Bergquist","year":"2009","journal-title":"Trends in parasitology"},{"issue":"11","key":"ref22","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.ijpara.2014.05.009","article-title":"Sensitivity of diagnostic tests for human soil-transmitted helminth infections: a meta-analysis in the absence of a true gold standard","volume":"44","author":"B Nikolay","year":"2014","journal-title":"International journal for parasitology"},{"issue":"7","key":"ref23","doi-asserted-by":"crossref","first-page":"e0004737","DOI":"10.1371\/journal.pntd.0004737","article-title":"Sustaining progress towards NTD elimination: an opportunity to leverage lymphatic filariasis elimination programs to interrupt transmission of soil-transmitted helminths","volume":"10","author":"AR Means","year":"2016","journal-title":"PLoS neglected tropical diseases"},{"issue":"12","key":"ref24","doi-asserted-by":"crossref","first-page":"e2586","DOI":"10.1371\/journal.pntd.0002586","article-title":"Impact of six rounds of mass drug administration on Brugian filariasis and soil-transmitted helminth infections in eastern Indonesia","volume":"7","author":"T Supali","year":"2013","journal-title":"PLoS neglected tropical diseases"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"e1621","DOI":"10.1371\/journal.pntd.0001621","article-title":"Soil-transmitted helminth reinfection after drug treatment: a systematic review and meta-analysis","volume":"6","author":"T-W Jia","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1186\/s13071-015-1135-4","article-title":"What impact will the achievement of the current World Health Organisation targets for anthelmintic treatment coverage in children have on the intensity of soil transmitted helminth infections?","volume":"8","author":"J Truscott","year":"2015","journal-title":"Parasites & vectors"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s13071-017-1979-x","article-title":"Assessing the interruption of the transmission of human helminths with mass drug administration alone: optimizing the design of cluster randomized trials","volume":"10","author":"R Anderson","year":"2017","journal-title":"Parasites & vectors"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"e0006114","DOI":"10.1371\/journal.pntd.0006114","article-title":"Testing for soil-transmitted helminth transmission elimination: Analysing the impact of the sensitivity of different diagnostic tools","volume":"12","author":"M Werkman","year":"2018","journal-title":"PLoS neglected tropical diseases"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1186\/1756-3305-7-266","article-title":"Can chemotherapy alone eliminate the transmission of soil transmitted helminths?","volume":"7","author":"JE Truscott","year":"2014","journal-title":"Parasites & vectors"},{"key":"ref30","article-title":"Soil-transmitted helminth infections","author":"PM Jourdan","year":"2017","journal-title":"The Lancet"},{"issue":"7","key":"ref31","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.ijpara.2015.02.011","article-title":"Multiplex real-time PCR monitoring of intestinal helminths in humans reveals widespread polyparasitism in Northern Samar, the Philippines","volume":"45","author":"CA Gordon","year":"2015","journal-title":"International journal for parasitology"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"338","DOI":"10.4269\/ajtmh.2011.10-0499","article-title":"A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths","volume":"84","author":"M Basuni","year":"2011","journal-title":"The American journal of tropical medicine and hygiene"},{"key":"ref33","first-page":"267","article-title":"Triplex polymerase chain reaction assay for detection of major soil-transmitted helminths, Ascaris lumbricoides, Trichuris trichiura, Necator americanus, in fecal samples","volume":"45","author":"O Phuphisut","year":"2014","journal-title":"Southeast Asian J Trop Med Public Health"},{"issue":"5","key":"ref34","doi-asserted-by":"crossref","first-page":"e0006389","DOI":"10.1371\/journal.pntd.0006389","article-title":"(S) WASH-D for Worms: A pilot study investigating the differential impact of school-versus community-based integrated control programs for soil-transmitted helminths","volume":"12","author":"NE Clarke","year":"2018","journal-title":"PLOS Neglected Tropical Diseases"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"e0004380","DOI":"10.1371\/journal.pntd.0004380","article-title":"Application of a Multiplex Quantitative PCR to Assess Prevalence and Intensity Of Intestinal Parasite Infections in a Controlled Clinical Trial","volume":"10","author":"S Llewellyn","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"ref36","article-title":"Development and evaluation of a multiplex quantitative real-time PCR for hookworm species in human stool","author":"SF Hii","year":"2018","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"issue":"13\u201314","key":"ref37","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1016\/j.ijpara.2012.10.005","article-title":"Establishment of a robotic, high-throughput platform for the specific diagnosis of gastrointestinal nematode infections in sheep","volume":"42","author":"F Roeber","year":"2012","journal-title":"Int J Parasitol"},{"key":"ref38","article-title":"Statistical power analysis for the behavioral sciences","author":"J Cohen","year":"1988"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1177\/001316446002000104","article-title":"A coefficient of agreement for nominal scales","volume":"20","author":"J Cohen","year":"1960","journal-title":"Educational and psychological measurement"},{"issue":"3","key":"ref40","doi-asserted-by":"crossref","first-page":"508","DOI":"10.4269\/ajtmh.16-0266","article-title":"Molecular diagnostics for soil-transmitted helminths","volume":"95","author":"EM O'Connell","year":"2016","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"4","key":"ref41","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.mcp.2011.05.002","article-title":"DNA amplification approaches for the diagnosis of key parasitic helminth infections of humans","volume":"25","author":"CA Gordon","year":"2011","journal-title":"Molecular and cellular probes"},{"issue":"6","key":"ref42","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.4269\/ajtmh.12-0726","article-title":"A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations","volume":"88","author":"R Mejia","year":"2013","journal-title":"The American journal of tropical medicine and hygiene"},{"issue":"2","key":"ref43","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1590\/S0037-86822005000200016","article-title":"Comparison of the thick smear and Kato-Katz techniques for diagnosis of intestinal helminth infections","volume":"38","author":"FLN Santos","year":"2005","journal-title":"Revista da Sociedade Brasileira de Medicina Tropical"},{"issue":"3","key":"ref44","doi-asserted-by":"crossref","first-page":"e0006213","DOI":"10.1371\/journal.pntd.0006213","article-title":"Diagnostic tools for soil-transmitted helminths control and elimination programs: A pathway for diagnostic product development","volume":"12","author":"MD Lim","year":"2018","journal-title":"PLoS neglected tropical diseases"},{"issue":"3","key":"ref45","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1637\/7341-020805R.1","article-title":"Comparison of the specificity and sensitivity of PCR, nested PCR, and real-time PCR for the diagnosis of histomoniasis","volume":"49","author":"H Hafez","year":"2005","journal-title":"Avian diseases"},{"issue":"10","key":"ref46","doi-asserted-by":"crossref","first-page":"e78230","DOI":"10.1371\/journal.pone.0078230","article-title":"Intra-genomic variation in the ribosomal repeats of nematodes","volume":"8","author":"HM Bik","year":"2013","journal-title":"PLoS One"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0007363","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pntd.0007363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T18:29:45Z","timestamp":1588962585000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007363"}},"subtitle":[],"editor":[{"given":"H\u00e9l\u00e8ne","family":"Carabin","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,6,17]]},"references-count":46,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,6,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0007363","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0007363","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,17]]}}}