{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:57:02Z","timestamp":1787021822085,"version":"build-2736575974"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T00:00:00Z","timestamp":1576454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["404762\/2016-6"],"award-info":[{"award-number":["404762\/2016-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"crossref","award":["E-26\/260.041\/2019"],"award-info":[{"award-number":["E-26\/260.041\/2019"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0007915","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T13:24:34Z","timestamp":1576502674000},"page":"e0007915","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Identification and validation of specific B-cell epitopes of hantaviruses associated to hemorrhagic fever and renal syndrome"],"prefix":"10.1371","volume":"13","author":[{"given":"Fernando de Paiva","family":"Conte","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Corr\u00eaa","family":"Tinoco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9699-3320","authenticated-orcid":true,"given":"Thiago","family":"Santos Chaves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Carvalho de","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janaina","family":"Figueira Mansur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo","family":"Mohana-Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elba Regina Sampaio de","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia Cristina da Costa","family":"Neves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9772-849X","authenticated-orcid":true,"given":"Rodrigo Nunes","family":"Rodrigues-da-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"key":"pntd.0007915.ref001","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jviromet.2013.11.004","article-title":"Development of a SYBR Green I based one-step real-time PCR assay for the detection of Hantaan virus","volume":"196","author":"W Jiang","year":"2014","journal-title":"J Virol Methods"},{"issue":"8","key":"pntd.0007915.ref002","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/nrmicro3066","article-title":"Uncovering the mysteries of hantavirus infections","volume":"11","author":"A Vaheri","year":"2013","journal-title":"Nat Rev Microbiol"},{"issue":"9","key":"pntd.0007915.ref003","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.1016\/j.celrep.2016.02.005","article-title":"Structure of the Hantavirus Nucleoprotein Provides Insights into the Mechanism of RNA Encapsidation","volume":"14","author":"D Olal","year":"2016","journal-title":"Cell Rep"},{"issue":"1","key":"pntd.0007915.ref004","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jviromet.2013.04.021","article-title":"Rapid, whole blood diagnostic test for detecting anti-hantavirus antibody in rats","volume":"193","author":"T Amada","year":"2013","journal-title":"J Virol Methods"},{"issue":"1","key":"pntd.0007915.ref005","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s12250-016-3899-x","article-title":"Hantavirus infection: a global zoonotic challenge","volume":"32","author":"H Jiang","year":"2017","journal-title":"Virol Sin"},{"key":"pntd.0007915.ref006","first-page":"82","article-title":"Hemorrhagic fever with renal syndrome (HFRS)","volume":"36","author":"HW Lee","year":"1982","journal-title":"Scand J Infect Dis Suppl"},{"issue":"4","key":"pntd.0007915.ref007","doi-asserted-by":"crossref","first-page":"810","DOI":"10.4269\/ajtmh.1985.34.810","article-title":"Isolation of a Hantaan-related virus from Brazilian rats and serologic evidence of its widespread distribution in South America","volume":"34","author":"JW LeDuc","year":"1985","journal-title":"Am J Trop Med Hyg"},{"issue":"7","key":"pntd.0007915.ref008","doi-asserted-by":"crossref","DOI":"10.3390\/v11070652","article-title":"Wild Rats, Laboratory Rats, Pet Rats: Global Seoul Hantavirus Disease Revisited","volume":"11","author":"J Clement","year":"2019","journal-title":"Viruses"},{"issue":"1","key":"pntd.0007915.ref009","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1089\/vbz.2013.1378","article-title":"Infections by Leptospira interrogans, Seoul virus, and Bartonella spp. among Norway rats (Rattus norvegicus) from the urban slum environment in Brazil","volume":"14","author":"F Costa","year":"2014","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"9194","key":"pntd.0007915.ref010","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1016\/S0140-6736(05)76782-9","article-title":"Urban leptospirosis versus urban hantavirus infection in Brazil","volume":"354","author":"J Clement","year":"1999","journal-title":"Lancet"},{"key":"pntd.0007915.ref011","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.virusres.2013.12.040","article-title":"Serological diagnosis with recombinant N antigen for hantavirus infection","volume":"187","author":"K Yoshimatsu","year":"2014","journal-title":"Virus Res"},{"issue":"5","key":"pntd.0007915.ref012","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1128\/JCM.00072-10","article-title":"Truncated hantavirus nucleocapsid proteins for serotyping Sin Nombre, Andes, and Laguna Negra hantavirus infections in humans and rodents","volume":"48","author":"T Koma","year":"2010","journal-title":"J Clin Microbiol"},{"issue":"4","key":"pntd.0007915.ref013","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/jmv.1890460402","article-title":"Human B-cell epitopes of Puumala virus nucleocapsid protein, the major antigen in early serological response","volume":"46","author":"O Vapalahti","year":"1995","journal-title":"J Med Virol"},{"issue":"1","key":"pntd.0007915.ref014","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0166-0934(02)00282-3","article-title":"Diagnostic rapid tests for acute hantavirus infections: specific tests for Hantaan, Dobrava and Puumala viruses versus a hantavirus combination test","volume":"108","author":"H Hujakka","year":"2003","journal-title":"J Virol Methods"},{"issue":"3","key":"pntd.0007915.ref015","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1128\/JCM.35.3.600-608.1997","article-title":"Rapid and specific detection of Sin Nombre virus antibodies in patients with hantavirus pulmonary syndrome by a strip immunoblot assay suitable for field diagnosis","volume":"35","author":"B Hjelle","year":"1997","journal-title":"J Clin Microbiol"},{"issue":"5","key":"pntd.0007915.ref016","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1002\/jcb.25765","article-title":"Prediction of B Cell Epitopes Among Hantavirus Strains Causing Hemorragic Fever With Renal Syndrome","volume":"118","author":"S Kalaiselvan","year":"2017","journal-title":"J Cell Biochem"},{"issue":"8","key":"pntd.0007915.ref017","doi-asserted-by":"crossref","first-page":"2320","DOI":"10.1002\/jcb.25887","article-title":"Prediction of Pan-Specific B-Cell Epitopes From Nucleocapsid Protein of Hantaviruses Causing Hantavirus Cardiopulmonary Syndrome","volume":"118","author":"S Kalaiselvan","year":"2017","journal-title":"J Cell Biochem"},{"issue":"W1","key":"pntd.0007915.ref018","doi-asserted-by":"crossref","first-page":"W24","DOI":"10.1093\/nar\/gkx346","article-title":"BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"MC Jespersen","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"pntd.0007915.ref019","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1471-2105-8-4","article-title":"VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines","volume":"8","author":"IA Doytchinova","year":"2007","journal-title":"BMC Bioinformatics"},{"issue":"1999","key":"pntd.0007915.ref020","first-page":"95","article-title":"BioEdit: User-friendly biological sequence alignment editor and analysis program for windows 95\/98\/NT","volume":"41","author":"TA Hall","journal-title":"Nucleic Acids Symposium Series"},{"issue":"1\u20132","key":"pntd.0007915.ref021","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1399-3011.1996.tb00814.x","article-title":"Automated multiple peptide synthesis: improvements in obtaining quality peptides","volume":"47","author":"T Luu","year":"1996","journal-title":"Int J Pept Protein Res"},{"issue":"6","key":"pntd.0007915.ref022","first-page":"315","article-title":"Automated multiple peptide synthesis","volume":"5","author":"H Gausepohl","year":"1992","journal-title":"Pept Res"},{"issue":"Suppl 18","key":"pntd.0007915.ref023","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1186\/s12859-016-1339-4","article-title":"Homology modeling and molecular dynamics provide structural insights into tospovirus nucleoprotein","volume":"17","author":"RN Lima","year":"2016","journal-title":"BMC Bioinformatics"},{"key":"pntd.0007915.ref024","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"C Camacho","year":"2009","journal-title":"BMC Bioinformatics"},{"issue":"2","key":"pntd.0007915.ref025","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nmeth.1818","article-title":"HHblits: lightning-fast iterative protein sequence searching by HMM-HMM alignment","volume":"9","author":"M Remmert","year":"2011","journal-title":"Nat Methods"},{"issue":"Web Server issue","key":"pntd.0007915.ref026","doi-asserted-by":"crossref","first-page":"W252","DOI":"10.1093\/nar\/gku340","article-title":"SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information","volume":"42","author":"M Biasini","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"Suppl 1","key":"pntd.0007915.ref027","doi-asserted-by":"crossref","first-page":"S162","DOI":"10.1002\/elps.200900140","article-title":"Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: a historical perspective","volume":"30","author":"N Guex","year":"2009","journal-title":"Electrophoresis"},{"issue":"3","key":"pntd.0007915.ref028","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modelling by satisfaction of spatial restraints","volume":"234","author":"A Sali","year":"1993","journal-title":"J Mol Biol"},{"issue":"5016","key":"pntd.0007915.ref029","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1126\/science.1853201","article-title":"A method to identify protein sequences that fold into a known three-dimensional structure","volume":"253","author":"JU Bowie","year":"1991","journal-title":"Science"},{"issue":"6364","key":"pntd.0007915.ref030","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/356083a0","article-title":"Assessment of protein models with three-dimensional profiles","volume":"356","author":"R Luthy","year":"1992","journal-title":"Nature"},{"issue":"Pt 1","key":"pntd.0007915.ref031","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1107\/S0907444909042073","article-title":"MolProbity: all-atom structure validation for macromolecular crystallography","volume":"66","author":"VB Chen","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"issue":"Pt 4","key":"pntd.0007915.ref032","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"P Emsley","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"issue":"1","key":"pntd.0007915.ref033","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1186\/s12879-016-2012-2","article-title":"Clinical characterization of two severe cases of hemorrhagic fever with renal syndrome (HFRS) caused by hantaviruses Puumala and Dobrava-Belgrade genotype Sochi","volume":"16","author":"E Krautkramer","year":"2016","journal-title":"BMC Infect Dis"},{"issue":"2","key":"pntd.0007915.ref034","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/jmv.20448","article-title":"Puumala virus in Croatia in the 2002 HFRS outbreak","volume":"77","author":"L Cvetko","year":"2005","journal-title":"J Med Virol"},{"issue":"9013","key":"pntd.0007915.ref035","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1016\/S0140-6736(96)91717-1","article-title":"Hantaan and Puumala virus antibodies in blood donors in Samara, an HFRS-endemic region in European Russia","volume":"347","author":"OA Alexeyev","year":"1996","journal-title":"Lancet"},{"key":"pntd.0007915.ref036","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jcv.2016.10.004","article-title":"Hantavirus cardiopulmonary syndrome due to Puumala virus in Germany","volume":"84","author":"P Vollmar","year":"2016","journal-title":"J Clin Virol"},{"key":"pntd.0007915.ref037","first-page":"w13937","article-title":"Human hantavirus infections: epidemiology, clinical features, pathogenesis and immunology","volume":"144","author":"T Manigold","year":"2014","journal-title":"Swiss Med Wkly"},{"issue":"2","key":"pntd.0007915.ref038","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1128\/JVI.02523-15","article-title":"Crystal Structure of the Core Region of Hantavirus Nucleocapsid Protein Reveals the Mechanism for Ribonucleoprotein Complex Formation","volume":"90","author":"Y Guo","year":"2016","journal-title":"J Virol"},{"issue":"5","key":"pntd.0007915.ref039","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1002\/jmv.24118","article-title":"Co-circulation in a single biome of the Juquitiba and Araraquara hantavirus detected in human sera in a sub-tropical region of Brazil","volume":"87","author":"J de Araujo","year":"2015","journal-title":"J Med Virol"},{"key":"pntd.0007915.ref040","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.virusres.2014.01.015","article-title":"Hantaviruses and cardiopulmonary syndrome in South America","volume":"187","author":"LT Figueiredo","year":"2014","journal-title":"Virus Res"},{"issue":"8","key":"pntd.0007915.ref041","doi-asserted-by":"crossref","first-page":"e71610","DOI":"10.1371\/journal.pone.0071610","article-title":"Computational and experimental validation of B and T-cell epitopes of the in vivo immune response to a novel malarial antigen","volume":"8","author":"ES Bergmann-Leitner","year":"2013","journal-title":"PLoS One"},{"key":"pntd.0007915.ref042","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.molimm.2018.05.019","article-title":"Biochemical analysis and identification of linear B-cell epitopes from recombinant Sm21.7 antigen from Schistosoma mansoni","volume":"101","author":"CMF Rezende","year":"2018","journal-title":"Mol Immunol"},{"issue":"7","key":"pntd.0007915.ref043","doi-asserted-by":"crossref","first-page":"e0181177","DOI":"10.1371\/journal.pone.0181177","article-title":"Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies","volume":"12","author":"J Ti","year":"2017","journal-title":"PLoS One"},{"issue":"2","key":"pntd.0007915.ref044","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.vaccine.2018.10.016","article-title":"Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9","volume":"37","author":"RN Rodrigues-da-Silva","year":"2019","journal-title":"Vaccine"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007915","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T22:53:24Z","timestamp":1695509604000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007915"}},"subtitle":[],"editor":[{"given":"Abdallah M.","family":"Samy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,12,16]]},"references-count":44,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0007915","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,16]]}}}