{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T18:07:57Z","timestamp":1775498877722,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0007928","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000}}],"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:00:00Z","timestamp":1575504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0007928","type":"journal-article","created":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T19:00:12Z","timestamp":1575572412000},"page":"e0007928","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Challenges in diagnosing scrub typhus among hospitalized patients with undifferentiated fever at a national tertiary hospital in northern Vietnam"],"prefix":"10.1371","volume":"13","author":[{"given":"Shungo","family":"Katoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngo Chi","family":"Cuong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9926-8098","authenticated-orcid":true,"given":"Sugihiro","family":"Hamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pham Thanh","family":"Thuy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Do Duy","family":"Cuong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le Kim","family":"Anh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi Hien","family":"Anh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dang Duc","family":"Anh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiichiro","family":"Sando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motoi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michio","family":"Yasunami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9567-6764","authenticated-orcid":true,"given":"Keisuke","family":"Yoshihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7567-3248","authenticated-orcid":true,"given":"Lay-Myint","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4743-5987","authenticated-orcid":true,"given":"Daniel Henry","family":"Paris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1468-5296","authenticated-orcid":true,"given":"Koya","family":"Ariyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"issue":"9","key":"pntd.0007928.ref001","doi-asserted-by":"crossref","first-page":"e44269","DOI":"10.1371\/journal.pone.0044269","article-title":"Mapping the aetiology of non-malarial febrile illness in Southeast Asia through a systematic review\u2014terra incognita impairing treatment policies","volume":"7","author":"N Acestor","year":"2012","journal-title":"PLoS One"},{"issue":"9","key":"pntd.0007928.ref002","doi-asserted-by":"crossref","first-page":"e0005838","DOI":"10.1371\/journal.pntd.0005838","article-title":"Estimating the burden of scrub typhus: A systematic review","volume":"11","author":"A Bonell","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"pntd.0007928.ref003","doi-asserted-by":"crossref","first-page":"796","DOI":"10.4269\/ajtmh.1973.22.796","article-title":"Epidemiology of the acute fevers of unknown origin in South Vietnam: effect of laboratory support upon clinical diagnosis","volume":"22","author":"SJ Berman","year":"1973","journal-title":"Am J Trop Med Hyg"},{"issue":"11","key":"pntd.0007928.ref004","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1093\/trstmh\/tru145","article-title":"Scrub typhus in the northern provinces of Vietnam: an observational study of admissions to a national referral hospital","volume":"108","author":"B Nadjm","year":"2014","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"5","key":"pntd.0007928.ref005","doi-asserted-by":"crossref","first-page":"972","DOI":"10.4269\/ajtmh.14-0806","article-title":"Clinical and Epidemiological Characteristics of Scrub Typhus and Murine Typhus among Hospitalized Patients with Acute Undifferentiated Fever in Northern Vietnam","volume":"92","author":"S Hamaguchi","year":"2015","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"pntd.0007928.ref006","doi-asserted-by":"crossref","first-page":"e0005397","DOI":"10.1371\/journal.pntd.0005397","article-title":"Use of eschar swabbing for the molecular diagnosis and genotyping of Orientia tsutsugamushi causing scrub typhus in Quang Nam province, Vietnam","volume":"11","author":"N Le Viet","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"pntd.0007928.ref007","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1128\/JCM.00997-08","article-title":"Orientia tsutsugamushi bacteremia and cytokine levels in Vietnamese scrub typhus patients","volume":"47","author":"S Kramme","year":"2009","journal-title":"J Clin Microbiol"},{"issue":"1","key":"pntd.0007928.ref008","doi-asserted-by":"crossref","first-page":"26","DOI":"10.7326\/0003-4819-79-1-26","article-title":"Scrub typhus in South Vietnam. A study of 87 cases","volume":"79","author":"SJ Berman","year":"1973","journal-title":"Ann Intern Med"},{"issue":"3","key":"pntd.0007928.ref009","doi-asserted-by":"crossref","first-page":"368","DOI":"10.4269\/ajtmh.2010.09-0233","article-title":"Diagnosis of scrub typhus","volume":"82","author":"GC Koh","year":"2010","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"pntd.0007928.ref010","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1128\/CVI.00553-15","article-title":"Diagnostic Accuracy of the InBios Scrub Typhus Detect Enzyme-Linked Immunoassay for the Detection of IgM Antibodies in Northern Thailand","volume":"23","author":"SD Blacksell","year":"2015","journal-title":"Clin Vaccine Immunol"},{"issue":"5","key":"pntd.0007928.ref011","doi-asserted-by":"crossref","first-page":"e0114930","DOI":"10.1371\/journal.pone.0114930","article-title":"How to Determine the Accuracy of an Alternative Diagnostic Test when It Is Actually Better than the Reference Tests: A Re-Evaluation of Diagnostic Tests for Scrub Typhus Using Bayesian LCMs","volume":"10","author":"C Lim","year":"2015","journal-title":"PLoS One"},{"issue":"6","key":"pntd.0007928.ref012","first-page":"580","article-title":"A ten years experience on diagnosis of rickettsial diseases using the indirect immunoperoxidase methods","volume":"35","author":"T. Suto","year":"1991","journal-title":"Acta Virol"},{"key":"pntd.0007928.ref013","first-page":"95","volume-title":"Diagnostics and Therapeutics for Tick-Borne Bacterial Diseases","author":"H Fujita","year":"2012"},{"key":"pntd.0007928.ref014","first-page":"109","volume-title":"Diagnostics and Therapeutics for Tick-Borne Bacterial Diseases","author":"H Fujita","year":"2013"},{"issue":"3","key":"pntd.0007928.ref015","doi-asserted-by":"crossref","first-page":"74","DOI":"10.3390\/tropicalmed3030074","article-title":"Serological Cross-Reactivity among Orientia tsutsugamushi Serotypes but Not with Rickettsia japonica in Japan","volume":"3","author":"E Sando","year":"2018","journal-title":"Trop Med Infect Dis"},{"issue":"4","key":"pntd.0007928.ref016","doi-asserted-by":"crossref","first-page":"351","DOI":"10.4269\/ajtmh.2004.70.351","article-title":"Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi","volume":"70","author":"J Jiang","year":"2004","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"pntd.0007928.ref017","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1089\/vbz.2012.1112","article-title":"Diversity of the 47-kD HtrA nucleic acid and translated amino acid sequences from 17 recent human isolates of Orientia","volume":"13","author":"J Jiang","year":"2013","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"6","key":"pntd.0007928.ref018","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1128\/JCM.31.6.1637-1640.1993","article-title":"Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction","volume":"31","author":"Y Furuya","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"12","key":"pntd.0007928.ref019","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis version 6.0","volume":"30","author":"K Tamura","year":"2013","journal-title":"Mol Biol Evol"},{"issue":"3","key":"pntd.0007928.ref020","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"K Tamura","year":"1993","journal-title":"Mol Biol Evol"},{"issue":"2","key":"pntd.0007928.ref021","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/BF02101694","article-title":"Dating of the human-ape splitting by a molecular clock of mitochondrial DNA","volume":"22","author":"M Hasegawa","year":"1985","journal-title":"J Mol Evol"},{"key":"pntd.0007928.ref022","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2019"},{"key":"pntd.0007928.ref023","first-page":"753","article-title":"Bach Mai Hospital Guidelines for diagnosis and treatment of internal medicine [in Vietnamese]","author":"NQ Anh","year":"2013","journal-title":"NXB Y hoc"},{"issue":"12","key":"pntd.0007928.ref024","doi-asserted-by":"crossref","first-page":"e909","DOI":"10.1371\/journal.pntd.0000909","article-title":"Contrasting spatial distribution and risk factors for past infection with scrub typhus and murine typhus in Vientiane City, Lao PDR","volume":"4","author":"J Vallee","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"pntd.0007928.ref025","doi-asserted-by":"crossref","first-page":"e0007158","DOI":"10.1371\/journal.pntd.0007158","article-title":"The validity of diagnostic cut-offs for commercial and in-house scrub typhus IgM and IgG ELISAs: A review of the evidence","volume":"13","author":"K Saraswati","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"pntd.0007928.ref026","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3390\/tropicalmed3030095","article-title":"Diagnostic Accuracy of the InBios Scrub Typhus Detect ELISA for the Detection of IgM Antibodies in Chittagong, Bangladesh","volume":"3","author":"S Blacksell","year":"2018","journal-title":"Tropical Medicine and Infectious Disease"},{"issue":"3","key":"pntd.0007928.ref027","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/trstmh\/trx022","article-title":"The genotypes of Orientia tsutsugamushi, identified in scrub typhus patients in northern Vietnam","volume":"111","author":"HLK Nguyen","year":"2017","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"pntd.0007928.ref028","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.meegid.2011.01.004","article-title":"Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity","volume":"15","author":"V Duong","year":"2013","journal-title":"Infect Genet Evol"},{"issue":"7","key":"pntd.0007928.ref029","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.1128\/IAI.58.7.2076-2084.1990","article-title":"The 56-kilodalton major protein antigen of Rickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope","volume":"58","author":"CK Stover","year":"1990","journal-title":"Infect Immun"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0007928","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T16:43:46Z","timestamp":1612975426000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007928"}},"subtitle":[],"editor":[{"given":"Allen L.","family":"Richards","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,12,5]]},"references-count":29,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0007928","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0007928","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,5]]}}}