{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:27:29Z","timestamp":1790908049781,"version":"4.1.0"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T00:00:00Z","timestamp":1577404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"DOI":"10.1371\/journal.pntd.0007946","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T18:23:54Z","timestamp":1577471034000},"page":"e0007946","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Nested PCR and the TaqMan SNP Genotyping Assay enhanced the sensitivity of drug resistance testing of Mycobacterium leprae using clinical specimens of leprosy patients"],"prefix":"10.1371","volume":"13","author":[{"given":"Xiaohua","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuangang","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianchao","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5792-6299","authenticated-orcid":true,"given":"Yan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,12,27]]},"reference":[{"key":"pntd.0007946.ref001","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/s41467-017-02576-z","article-title":"Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae","volume":"9","author":"A Benjak","year":"2018","journal-title":"Nat Commun"},{"key":"pntd.0007946.ref002","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1093\/cid\/ciw572","article-title":"Transmission of drug-resistant leprosy in guinea-conakry detected using molecular epidemiological approaches","volume":"63","author":"C Avanzi","year":"2016","journal-title":"Clin Infect Dis"},{"key":"pntd.0007946.ref003","first-page":"317","article-title":"Global leprosy situation, 2012","volume":"87","author":"World Health Organization","year":"2012","journal-title":"Wkly Epidemiol Rec"},{"key":"pntd.0007946.ref004","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.clindermatol.2015.10.019","article-title":"Drug-resistant Mycobacterium leprae","volume":"34","author":"PR Saunderson","year":"2015","journal-title":"Clin Dermatol"},{"key":"pntd.0007946.ref005","first-page":"75","article-title":"Global surveillance system to monitor the development of drug resistance in Mycobacterium leprae","volume":"6","author":"M Matsuoka","year":"2015","journal-title":"Res Rep Trop Med"},{"key":"pntd.0007946.ref006","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0140-6736(05)60888-4","article-title":"Multidrug-resistance to dapsone, rifampicin, and ofloxacin in Mycobacterium leprae","volume":"349","author":"E Cambau","year":"1997","journal-title":"Lancet"},{"key":"pntd.0007946.ref007","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.1128\/AAC.45.12.3635-3639.2001","article-title":"Multidrug resistant Mycobacterium leprae from patients with leprosy","volume":"45","author":"S Maeda","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"pntd.0007946.ref008","first-page":"452","article-title":"A Mycobacterium leprae isolate resistant to dapsone, rifampin, ofloxacin and sparfloxacin","volume":"68","author":"M Matsuoka","year":"2000","journal-title":"Int J Lepr Other Mycobact Dis"},{"key":"pntd.0007946.ref009","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1489\/1544-581X(2003)71<240:ASCOMM>2.0.CO;2","article-title":"A second case of multidrug-resistant Mycobacterium leprae isolated from a Japanese patient with relapsed lepromatous leprosy","volume":"71","author":"M Matsuoka","year":"2003","journal-title":"Int J Lepr Other Mycobact Dis"},{"key":"pntd.0007946.ref010","doi-asserted-by":"crossref","first-page":"343","DOI":"10.47276\/lr.78.4.343","article-title":"The frequency of drug resistance mutations in Mycobacterium leprae isolates in untreated and relapsed leprosy patients from Myanmar, Indonesia and the Philippines","volume":"78","author":"M Matsuoka","year":"2007","journal-title":"Lepr Rev"},{"key":"pntd.0007946.ref011","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1128\/JCM.06561-11","article-title":"Drug and multidrug resistance among Mycobacterium leprae isolates from Brazilian relapsed leprosy patients","volume":"50","author":"ADS Rocha","year":"2012","journal-title":"J Clin Microbiol"},{"key":"pntd.0007946.ref012","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.jinf.2004.03.012","article-title":"Mutations in genes related to drug resistance in Mycobacterium leprae isolates from leprosy patients in Korea","volume":"50","author":"EY You","year":"2005","journal-title":"J Infect"},{"key":"pntd.0007946.ref013","volume-title":"Guidelines for global surveillance of drug resistance in leprosy","author":"WHO Regional Office for South-East Asia","year":"2009"},{"key":"pntd.0007946.ref014","doi-asserted-by":"crossref","first-page":"e1739","DOI":"10.1371\/journal.pntd.0001739","article-title":"Detection of antibiotic resistance in leprosy using GenoType LepraeDR, a novel ready-to-use molecular test","volume":"6","author":"E Cambau","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0007946.ref015","doi-asserted-by":"crossref","first-page":"e0005506","DOI":"10.1371\/journal.pntd.0005506","article-title":"qPCR-high resolution melt analysis for drug susceptibility testing of Mycobacterium leprae directly from clinical specimens of leprosy patients","volume":"11","author":"S Araujo","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0007946.ref016","doi-asserted-by":"crossref","first-page":"e0132253","DOI":"10.1371\/journal.pone.0132253","article-title":"Nested PCR biases in interpreting microbial community structure in 16S rRNA gene sequence datasets","volume":"10","author":"G Yu","year":"2015","journal-title":"PLoS One"},{"key":"pntd.0007946.ref017","doi-asserted-by":"crossref","first-page":"e127","DOI":"10.1093\/cid\/ciq217","article-title":"Analysis of drug-resistant strains of Mycobacterium leprae in an endemic area of Vietnam","volume":"52","author":"M Kai","year":"2011","journal-title":"Clin Infect Dis"},{"key":"pntd.0007946.ref018","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1128\/AAC.01212-10","article-title":"Mutation analysis of the Mycobacterium leprae folP1 gene and dapsone resistance","volume":"55","author":"N Nakata","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"pntd.0007946.ref019","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jdermsci.2012.05.002","article-title":"Present situation of leprosy in Japan, 2006\u20132010: Analysis of drug resistance in new registered and relapsed cases by molecular biological methods","volume":"67","author":"S Mori","year":"2012","journal-title":"J Dermatol Sci"},{"key":"pntd.0007946.ref020","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1111\/ced.12665","article-title":"Drug resistance in Mycobacterium leprae from patients with leprosy in China","volume":"40","author":"D Liu","year":"2015","journal-title":"Clin Exp Dermatol"},{"key":"pntd.0007946.ref021","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/j.cmi.2018.02.022","article-title":"Antimicrobial resistance in leprosy: Results of the first prospective open survey conducted by a WHO surveillance network for the period 2009\u201315","volume":"24","author":"E Cambau","year":"2018","journal-title":"Clin Microbiol Infect"},{"key":"pntd.0007946.ref022","doi-asserted-by":"crossref","first-page":"e0005041","DOI":"10.1371\/journal.pntd.0005041","article-title":"Leprosy drug resistance surveillance in Colombia: The experience of a sentinel country","volume":"10","author":"C Beltr\u00e1n-Alzate","year":"2016","journal-title":"PLoS Negl Trop Dis"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007946","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T09:12:16Z","timestamp":1613293936000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0007946"}},"subtitle":[],"editor":[{"given":"Ruifu","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,12,27]]},"references-count":22,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0007946","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,27]]}}}