{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:49:09Z","timestamp":1787827749338,"version":"build-2784847793"},"update-to":[{"DOI":"10.1371\/journal.pntd.0008308","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000}}],"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T00:00:00Z","timestamp":1606262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/N013514\/1"],"award-info":[{"award-number":["MR\/N013514\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L019035\/1"],"award-info":[{"award-number":["BB\/L019035\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L019035\/1"],"award-info":[{"award-number":["BB\/L019035\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L019035\/1"],"award-info":[{"award-number":["BB\/L019035\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Human African Trypanosomiasis (HAT) is a potentially fatal parasitic infection caused by the trypanosome sub-species\n                    <jats:italic>Trypanosoma brucei gambiense<\/jats:italic>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>b<\/jats:italic>\n                    .\n                    <jats:italic>rhodesiense<\/jats:italic>\n                    transmitted by tsetse flies. Currently, global HAT case numbers are reaching less than 1 case per 10,000 people in many disease foci. As such, there is a need for simple screening tools and strategies to replace active screening of the human population which can be maintained post-elimination for Gambian HAT and long-term for Rhodesian HAT. Here, we describe the proof of principle application of a novel high-resolution melt assay for the xenomonitoring of\n                    <jats:italic>Trypanosoma brucei gambiense<\/jats:italic>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>b<\/jats:italic>\n                    .\n                    <jats:italic>rhodesiense<\/jats:italic>\n                    in tsetse. Both novel and previously described primers which target species-specific single copy genes were used as part of a multiplex qPCR. An additional primer set was included in the multiplex to determine if samples had sufficient genomic material for detecting genes present in low copy number. The assay was evaluated on 96 wild-caught tsetse previously identified to be positive for\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>brucei s<\/jats:italic>\n                    .\n                    <jats:italic>l<\/jats:italic>\n                    . of which two were known to be positive for\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>b<\/jats:italic>\n                    .\n                    <jats:italic>rhodesiense<\/jats:italic>\n                    . The assay was found to be highly specific with no cross-reactivity with non-target trypanosome species and the assay limit of detection was 10\n                    <jats:sup>4<\/jats:sup>\n                    tryps\/mL. The qPCR successfully identified three\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>b<\/jats:italic>\n                    .\n                    <jats:italic>rhodesiense<\/jats:italic>\n                    positive flies, in agreement with the reference species-specific PCRs. This assay provides an alternative to running multiple PCRs when screening for pathogenic sub-species of\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>brucei s<\/jats:italic>\n                    .\n                    <jats:italic>l<\/jats:italic>\n                    . and produces results in less than 2 hours, avoiding gel electrophoresis and subjective analysis. This method could provide a component of a simple and efficient method of screening large numbers of tsetse flies in known HAT foci or in areas at risk of recrudescence or threatened by the changing distribution of both forms of HAT.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0008308","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T13:37:40Z","timestamp":1606311460000},"page":"e0008308","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["A pilot study demonstrating the identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using a multiplexed high-resolution melt qPCR"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7140-1077","authenticated-orcid":true,"given":"Gala","family":"Garrod","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0816-2835","authenticated-orcid":true,"given":"Emily R.","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica K.","family":"Lingley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4141-8625","authenticated-orcid":true,"given":"Isabel","family":"Saldanha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Torr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas J.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"pntd.0008308.ref001","unstructured":"World Health Organization. Trypanosomiasis, human African (sleeping sickness) [Internet]. [cited 2018 Sep 6]. http:\/\/www.who.int\/news-room\/fact-sheets\/detail\/trypanosomiasis-human-african-(sleeping-sickness)"},{"issue":"10","key":"pntd.0008308.ref002","doi-asserted-by":"crossref","first-page":"e3244","DOI":"10.1371\/journal.pntd.0003244","article-title":"First WHO Meeting of Stakeholders on Elimination of Gambiense Human African Trypanosomiasis","volume":"8","author":"P Holmes","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0008308.ref003","unstructured":"World Health Organization. Accelerating work to overcome the global impact of neglected tropical diseases: a roadmap for implementation: executive summary. 2012;"},{"key":"pntd.0008308.ref004","unstructured":"World Health Organization. Control and surveillance of human African trypanosomiasis. [cited 2018 Jun 18]; http:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/95732\/9789241209847_eng.pdf?sequence=1&isAllowed=y"},{"issue":"1","key":"pntd.0008308.ref005","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1752-1505-5-7","article-title":"Challenges of controlling sleeping sickness in areas of violent conflict: experience in the Democratic Republic of Congo","volume":"5","author":"J Tong","year":"2011","journal-title":"Confl Health"},{"issue":"1","key":"pntd.0008308.ref006","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1186\/s13071-017-2487-8","article-title":"Serological tests for gambiense human African trypanosomiasis detect antibodies in cattle","volume":"10","author":"E Matovu","year":"2017","journal-title":"Parasit Vectors"},{"issue":"2","key":"pntd.0008308.ref007","doi-asserted-by":"crossref","first-page":"e0003480","DOI":"10.1371\/journal.pntd.0003480","article-title":"Accuracy of Individual Rapid Tests for Serodiagnosis of Gambiense Sleeping Sickness in West Africa","volume":"9","author":"V Jamonneau","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pntd.0008308.ref008","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004608","article-title":"Sensitivity and Specificity of a Prototype Rapid Diagnostic Test for the Detection of Trypanosoma brucei gambiense Infection: A Multi-centric Prospective Study","volume":"10","author":"S Bisser","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"pntd.0008308.ref009","doi-asserted-by":"crossref","first-page":"e359","DOI":"10.1016\/S2214-109X(14)70203-7","article-title":"Sensitivity and specificity of HAT Sero-K-SeT, a rapid diagnostic test for serodiagnosis of sleeping sickness caused by Trypanosoma brucei gambiense: A case-control study","volume":"2","author":"P B\u00fcscher","year":"2014","journal-title":"Lancet Glob Heal"},{"key":"pntd.0008308.ref010","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ebiom.2017.10.032","article-title":"A Phase III Diagnostic Accuracy Study of a Rapid Diagnostic Test for Diagnosis of Second-Stage Human African Trypanosomiasis in the Democratic Republic of the Congo","volume":"27","author":"M Boelaert","year":"2018","journal-title":"EBioMedicine"},{"key":"pntd.0008308.ref011","first-page":"4","volume-title":"Acta Tropica","author":"J Keating","year":"2015"},{"issue":"4","key":"pntd.0008308.ref012","doi-asserted-by":"crossref","first-page":"593","DOI":"10.4269\/ajtmh.2007.77.593","article-title":"A critical appraisal of molecular xenomonitoring as a tool for assessing progress toward elimination of lymphatic filariasis","volume":"77","author":"HA Farid","year":"2007","journal-title":"Am J Trop Med Hyg"},{"issue":"8","key":"pntd.0008308.ref013","doi-asserted-by":"crossref","first-page":"e3087","DOI":"10.1371\/journal.pntd.0003087","article-title":"Molecular Xenomonitoring Using Mosquitoes to Map Lymphatic Filariasis after Mass Drug Administration in American Samoa","volume":"8","author":"MA Schmaedick","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"pntd.0008308.ref014","doi-asserted-by":"crossref","first-page":"e0005108","DOI":"10.1371\/journal.pntd.0005108","article-title":"Lymphatic Filariasis Elimination in American Samoa: Evaluation of Molecular Xenomonitoring as a Surveillance Tool in the Endgame","volume":"10","author":"CL Lau","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0008308.ref015","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0001-706X(98)00014-X","article-title":"Detection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in Cameroon","volume":"70","author":"I Morlais","year":"1998","journal-title":"Acta Trop"},{"issue":"6","key":"pntd.0008308.ref016","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2471\/BLT.12.112706","article-title":"External quality assessment of Giemsa-stained blood film microscopy for the diagnosis of malaria and sleeping sickness in the Democratic Republic of the Congo","volume":"91","author":"P Mukadi","year":"2013","journal-title":"Bull World Health Organ"},{"issue":"1","key":"pntd.0008308.ref017","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1017\/S0031182000061023","article-title":"Detection of trypanosoma congolense and trypanosoma brucei subspecies by dna amplification using the polymerase chain reaction","volume":"99","author":"DR Moser","year":"1989","journal-title":"Parasitology"},{"issue":"1","key":"pntd.0008308.ref018","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0166-6851(01)00208-0","article-title":"A receptor-like flagellar pocket glycoprotein specific to Trypanosoma brucei gambiense","volume":"113","author":"M Berberof","year":"2001","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"pntd.0008308.ref019","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0166-6851(89)90189-8","article-title":"A gene expressed only in serum-resistant variants of Trypanosoma brucei rhodesiense","volume":"36","author":"C De Greef","year":"1989","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"pntd.0008308.ref020","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/0166-6851(94)90172-4","article-title":"The serum resistance-associated (SRA) gene of Trypanosoma brucei rhodesiense encodes a variant surface glycoprotein-like protein","volume":"68","author":"C De Greef","year":"1994","journal-title":"Mol Biochem Parasitol"},{"issue":"1","key":"pntd.0008308.ref021","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.exppara.2007.05.014","article-title":"A multiplex PCR that discriminates between Trypanosoma brucei brucei and zoonotic T. b. rhodesiense","volume":"118","author":"K Picozzi","year":"2008","journal-title":"Exp Parasitol"},{"key":"pntd.0008308.ref022","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.85.23.8914","article-title":"cDNA encoding the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei (glycolipid anchor\/variant surface glycoprotein)","volume":"Vol. 85","author":"D Hereld","year":"1988","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"3","key":"pntd.0008308.ref023","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0166-6851(89)90091-1","article-title":"Sequence and expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei","volume":"33","author":"M Carrington","year":"1989","journal-title":"Mol Biochem Parasitol"},{"key":"pntd.0008308.ref024","unstructured":"Winkle S. Gei\u00dfeln der Menschheit. Kulturgeschichte der Seuchen. Kult der Seuchen. 1997;D\u00fcsseldorf."},{"key":"pntd.0008308.ref025","article-title":"Genomic Organization, Chromosomal Localization, and Developmentally Regulated Expression of the Glycosyl-Phosphatidylinositol-Specific Phospholipase C of Trypanosoma brucei [Internet]","author":"K Mensa Wilmot","year":"1990","journal-title":"MOLECULAR AND CELLULAR BIOLOGY"},{"issue":"1","key":"pntd.0008308.ref026","doi-asserted-by":"crossref","DOI":"10.1186\/1756-3305-6-112","article-title":"Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) through the implementation of a High-Resolution Melting (HRM) genotyping assay","volume":"6","author":"SL Higuera","year":"2013","journal-title":"Parasites and Vectors"},{"issue":"4","key":"pntd.0008308.ref027","doi-asserted-by":"crossref","first-page":"315","DOI":"10.4103\/0972-9062.256568","article-title":"High resolution melting analysis as an accurate method for identifying Leishmania infantum in canine serum samples","volume":"55","author":"A Hosseini-Safa","year":"2018","journal-title":"J Vector Borne Dis"},{"issue":"1","key":"pntd.0008308.ref028","doi-asserted-by":"crossref","first-page":"e581","DOI":"10.1371\/journal.pntd.0000581","article-title":"Detection and Identification of Old World Leishmania by High Resolution Melt Analysis","volume":"4","author":"D Talmi-Frank","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0008308.ref029","unstructured":"Radwanska M, Claes F, Magez S, Magnus E, P\u00e9rez-Morga D, Pays E, et al. Novel primer sequences for Polymerase Chain Reaction-based detection of [Internet]. 2014 [cited 2018 Sep 10]. https:\/\/www.researchgate.net\/publication\/11055435."},{"issue":"1","key":"pntd.0008308.ref030","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0166-6851(82)90049-4","article-title":"On the DNA content and ploidy of trypanosomes","volume":"6","author":"P Borst","year":"1982","journal-title":"Mol Biochem Parasitol"},{"key":"pntd.0008308.ref031","doi-asserted-by":"crossref","unstructured":"JS Lord, RS. Lea, FK. Allan, M Byamungu, DR Hall, J Lingley, F Mramba, E Paxton, GA Vale, JW Hargrove, LJ Morrison, SJ Torr HA. Assessing the effect of insecticide-treated cattle on tsetse abundance and trypanosome transmission at the wildlife-livestock interface in Serengeti, Tanzania. bioRxiv doi. 2020;","DOI":"10.1101\/2020.04.14.040873"},{"key":"pntd.0008308.ref032","doi-asserted-by":"crossref","unstructured":"Masiga DK, Smyth AJ, Hayes P, Bromidge TJ, Gibson WC. SENSITIVE DETECTION OF TRYPANOSOMES IN TSETSE FLIES BY DNA AMPLIFICATION. 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Predicting the effect of climate change on African trypanosomiasis: integrating epidemiology with parasite and vector biology. [cited 2020 Feb 18]; http:\/\/rsif.royalsocietypublishing.org."},{"issue":"9282","key":"pntd.0008308.ref039","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/S0140-6736(01)05778-6","article-title":"The origins of a new Trypanosoma brucei rhodesiense sleeping sickness outbreak in eastern Uganda","volume":"358","author":"EM F\u00e8vre","year":"2001","journal-title":"Lancet"},{"key":"pntd.0008308.ref040","unstructured":"Picozzi K, F\u00e8vre EM, Odiit M, Carrington M, Eisler MC, Maudlin I, et al. Sleeping sickness in Uganda: a thin line between two fatal diseases. 2001 [cited 2020 Feb 17]; http:\/\/www.bmj.com\/."},{"issue":"7","key":"pntd.0008308.ref041","doi-asserted-by":"crossref","first-page":"e0005792","DOI":"10.1371\/journal.pntd.0005792","article-title":"Adding tsetse control to medical activities contributes to decreasing transmission of sleeping sickness in the Mandoul focus (Chad)","volume":"11","author":"MH Mahamat","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"8","key":"pntd.0008308.ref042","doi-asserted-by":"crossref","first-page":"e0003727","DOI":"10.1371\/journal.pntd.0003727","article-title":"Reducing Human-Tsetse Contact Significantly Enhances the Efficacy of Sleeping Sickness Active Screening Campaigns: A Promising Result in the Context of Elimination","volume":"9","author":"F Courtin","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0008308.ref043","first-page":"1","article-title":"Impact of Tiny Targets on Glossina fuscipes quanzensis, the primary vector of Human African Trypanosomiasis in the Democratic Republic of Congo","author":"I Tirados","year":"2020","journal-title":"bioRxiv"},{"issue":"8","key":"pntd.0008308.ref044","doi-asserted-by":"crossref","first-page":"e0003822","DOI":"10.1371\/journal.pntd.0003822","article-title":"Tsetse Control and Gambian Sleeping Sickness; Implications for Control Strategy","volume":"9","author":"I Tirados","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"1\u20132","key":"pntd.0008308.ref045","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.actatropica.2006.10.002","article-title":"Trypanosome identification in wild tsetse populations in Tanzania using generic primers to amplify the ribosomal RNA ITS-1 region","volume":"100","author":"ER Adams","year":"2006","journal-title":"Acta Trop"},{"issue":"2","key":"pntd.0008308.ref046","doi-asserted-by":"crossref","first-page":"167","DOI":"10.2217\/17460913.3.2.167","article-title":"New molecular tools for the identification of trypanosome species","volume":"3","author":"ER Adams","year":"2008","journal-title":"Futur Microbiol"},{"issue":"5","key":"pntd.0008308.ref047","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.ijpara.2007.09.006","article-title":"African trypanosomiasis: Sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA","volume":"38","author":"ZK Njiru","year":"2008","journal-title":"Int J Parasitol"},{"issue":"11","key":"pntd.0008308.ref048","doi-asserted-by":"crossref","first-page":"e865","DOI":"10.1371\/journal.pntd.0000865","article-title":"LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats","volume":"4","author":"SL Wastling","year":"2010","journal-title":"PLoS Negl Trop Dis"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0008308","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0008308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T19:58:55Z","timestamp":1607543935000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0008308"}},"subtitle":[],"editor":[{"given":"Enock","family":"Matovu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,25]]},"references-count":48,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0008308","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.04.21.052928","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,25]]}}}