{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:59:36Z","timestamp":1790751576984,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0008776","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000}}],"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T00:00:00Z","timestamp":1602720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["31830087"],"award-info":[{"award-number":["31830087"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81829004"],"award-info":[{"award-number":["81829004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"the National Institutes of Health, USA","doi-asserted-by":"crossref","award":["AI136850"],"award-info":[{"award-number":["AI136850"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Natural Science Foundation of Guangdong Province","award":["2014A030312016"],"award-info":[{"award-number":["2014A030312016"]}]},{"DOI":"10.13039\/501100009334","name":"Pearl River S and T Nova Program of Guangzhou","doi-asserted-by":"crossref","award":["201807010005"],"award-info":[{"award-number":["201807010005"]}],"id":[{"id":"10.13039\/501100009334","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100009334","name":"Pearl River S and T Nova Program of Guangzhou","doi-asserted-by":"crossref","award":["201803040006"],"award-info":[{"award-number":["201803040006"]}],"id":[{"id":"10.13039\/501100009334","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by <jats:italic>Aedes<\/jats:italic> mosquitoes. <jats:italic>Aedes albopictus<\/jats:italic> is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic>, while studies on <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> are very limited. To explore vertical transmission in <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic>, a series of laboratory studies were carried out.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>In this study, <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> were blood-fed with ZIKV-infectious blood, and the ovaries and offspring viral infection rates were analyzed by reverse transcription PCR (RT-PCR), real-time reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC). ZIKV was detected in the ovaries and oviposited eggs in two gonotrophic cycles. The minimum filial egg infection rates in two gonotrophic cycles were 2.06% and 0.69%, and the effective population transmission rate was 1.87%. The hatching, pupation, and emergence rates of infected offspring were not significantly different from those of uninfected offspring, indicating that ZIKV did not prevent the offspring from completing the growth and development process. ZIKV was detected in three of thirteen C57BL\/6 suckling mice bitten by ZIKV-positive F<jats:sub>1<\/jats:sub> females, and the viremia persisted for at least seven days.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>ZIKV can be vertically transmitted in <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> via transovarial transmission. The vertical transmission rates in F<jats:sub>1<\/jats:sub> eggs and adults were 2.06% and 1.87%, respectively. Even though the vertical transmission rates were low, the female mosquitoes infected via the congenital route horizontally transmitted ZIKV to suckling mice through bloodsucking. This is the first experimental evidence of offspring with vertically transmitted ZIKV initiating new horizontal transmission. The present study deepens the understanding of the vertical transmission of flaviviruses in <jats:italic>Aedes<\/jats:italic> mosquitoes and sheds light on the prevention and control of mosquito-borne diseases.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0008776","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T19:00:23Z","timestamp":1602788423000},"page":"e0008776","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":39,"title":["Vertical transmission of zika virus in Aedes albopictus"],"prefix":"10.1371","volume":"14","author":[{"given":"Zetian","family":"Lai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinbao","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8760-2925","authenticated-orcid":true,"given":"Guiyun","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7927-691X","authenticated-orcid":true,"given":"Xiao-Guang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"issue":"9","key":"pntd.0008776.ref001","doi-asserted-by":"crossref","first-page":"20720","DOI":"10.2807\/1560-7917.ES2014.19.9.20720","article-title":"Zika virus infection complicated by Guillain-Barre syndrome\u2013case report, French Polynesia, December 2013.","volume":"19","author":"E Oehler","year":"2014","journal-title":"Euro Surveill."},{"issue":"10030","key":"pntd.0008776.ref002","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1016\/S0140-6736(16)30253-7","article-title":"Positive IgM for Zika virus in the cerebrospinal fluid of 30 neonates with microcephaly in Brazil","volume":"387","author":"MT Cordeiro","year":"2016","journal-title":"The Lancet"},{"key":"pntd.0008776.ref003","unstructured":"World Health Organization. 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