{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T10:28:38Z","timestamp":1792751318721,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0008815","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000}}],"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T00:00:00Z","timestamp":1627430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPPGD759"],"award-info":[{"award-number":["OPPGD759"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD078912"],"award-info":[{"award-number":["R01HD078912"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010869","name":"Stanford Woods Institute for the Environment","doi-asserted-by":"publisher","award":["Goldman Graduate Fellowship"],"award-info":[{"award-number":["Goldman Graduate Fellowship"]}],"id":[{"id":"10.13039\/100010869","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-114747"],"award-info":[{"award-number":["DGE-114747"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Improved sanitation has been hypothesized to reduce soil-transmitted helminth (STH) infections by reducing the prevalence and concentration of STH eggs\/larvae in soil. We evaluated the effect of a randomized sanitation program (providing households with an improved dual-pit latrine, tools for child\/animal feces management, and behavioral messaging) on reducing the prevalence and concentration of STH eggs in soil from household courtyards. We collected soil samples from 1405 households enrolled in the sanitation intervention (n = 419) and control (n = 914) groups of a cluster-randomized controlled trial (WASH Benefits) in rural Bangladesh approximately 2 years after the initiation of the interventions. We analyzed samples for <jats:italic>Ascaris lumbricoides<\/jats:italic>, <jats:italic>Trichuris trichiura<\/jats:italic>, and hookworm eggs by microscopy. We estimated prevalence ratios (PR) and egg count ratio (ECR) to compare the prevalence of STH eggs and arithmetic and geometric mean egg counts for STH eggs per gram of soil in the sanitation and control arms. Among intervention households, latrines achieved high and sustained user uptake by adults while child open defecation remained common and most households did not dispose of child feces hygienically. In courtyard soil from control households, the prevalence of any STH eggs was 75.7% and the prevalence of any larvated STH eggs was 67.3%. <jats:italic>A<\/jats:italic>. <jats:italic>lumbricoides<\/jats:italic> was detected in 63.0% of control samples and <jats:italic>T<\/jats:italic>. <jats:italic>trichiura<\/jats:italic> in 55.7% of control samples; hookworm was not detected in any sample. In the control arm, the arithmetic mean egg count for any STH was 3.96 eggs\/dry gram, while the geometric mean was 1.58 eggs\/dry gram. There was no difference between the intervention and control groups in the prevalence of any STH eggs (PR = 0.98 (95% CI: 0.91, 1.05)) or mean egg counts (ECR = 0.08 (95% CI: -0.10, 0.26) for geometric mean and 0.07 (95% CI: -0.22, 0.37) for arithmetic mean). Adjusted models gave similar results. A compound-level sanitation intervention that provided improved latrines and tools for disposal of child and animal feces did not have an impact on STH eggs in soil. In order to effectively reduce the prevalence and concentration of STH eggs in the environment, sustained, widespread use of sanitation strategies to isolate and hygienically dispose of child and animal feces may need to complement traditional strategies for containment of adult human feces.<\/jats:p>\n<jats:p><jats:bold>Trial Registration:<\/jats:bold> <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/ct2\/show\/ClinicalTrials.gov%20identifier:%20%20NCT01590095\" xlink:type=\"simple\">NCT01590095<\/jats:ext-link>.<\/jats:p>","DOI":"10.1371\/journal.pntd.0008815","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T17:35:50Z","timestamp":1627493750000},"page":"e0008815","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Effect of sanitation improvements on soil-transmitted helminth eggs in courtyard soil from rural Bangladesh: Evidence from a cluster-randomized controlled trial"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9776-9355","authenticated-orcid":true,"given":"Laura H.","family":"Kwong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6231-998X","authenticated-orcid":true,"given":"Debashis","family":"Sen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharmin","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunny","family":"Shahriar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3631-3132","authenticated-orcid":true,"given":"Jade","family":"Benjamin-Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6105-7295","authenticated-orcid":true,"given":"Benjamin F.","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Hubbard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarker Masud","family":"Parvez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2810-9211","authenticated-orcid":true,"given":"Mahfuza","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7757-5641","authenticated-orcid":true,"given":"Leanne","family":"Unicomb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Mahbubur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3288-6956","authenticated-orcid":true,"given":"John M.","family":"Colford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen P.","family":"Luby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayse","family":"Ercumen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,7,28]]},"reference":[{"key":"pntd.0008815.ref001","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1756-3305-7-37","article-title":"Global numbers of infection and disease burden of soil transmitted helminth infections in 2010","volume":"7","author":"RL Pullan","year":"2014","journal-title":"Parasit Vectors"},{"key":"pntd.0008815.ref002","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.pt.2015.10.006","article-title":"A Critical Appraisal of Control Strategies for Soil-Transmitted Helminths","volume":"32","author":"SJ Campbell","year":"2016","journal-title":"Trends in Parasitology"},{"key":"pntd.0008815.ref003","first-page":"299","article-title":"Efficacy of Current Drugs Against Soil-Transmitted Helminth Infections: Systematic Review and Meta-analysis","author":"J Keiser","year":"2008","journal-title":"JAMA"},{"key":"pntd.0008815.ref004","doi-asserted-by":"crossref","first-page":"e948","DOI":"10.1371\/journal.pntd.0000948","article-title":"Assessment of the Anthelmintic Efficacy of Albendazole in School Children in Seven Countries Where Soil-Transmitted Helminths Are Endemic. 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Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"pntd.0008815.ref024","first-page":"200","article-title":"Determining the prevalence of intestinal parasites in three Orang Asli (Aborigines) communities in Perak, Malaysia","volume":"29","author":"B Sinniah","year":"2012","journal-title":"Tropical Biomedicine"},{"key":"pntd.0008815.ref025","first-page":"6","article-title":"Contamination of soil with helminth parasite eggs in Nepal.","volume":"31","author":"SK Rai","year":"2000","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"pntd.0008815.ref026","first-page":"166","article-title":"Soil transmitted helminthiasis in Kathmandu, Nepal","volume":"9","author":"A Shrestha","year":"2007","journal-title":"Nepal Medical College Journal"},{"key":"pntd.0008815.ref027","first-page":"495","article-title":"Soil contamination by parasite eggs in rural village in the Philippines.","volume":"30","author":"S Horiuchi","year":"2013","journal-title":"Tropical Biomedicine"},{"key":"pntd.0008815.ref028","first-page":"64","article-title":"Soil contamination and infections by soil-transmitted helminths in an endemic village in southern Thailand","volume":"30","author":"V Chongsuvivatwong","year":"1999","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"pntd.0008815.ref029","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1093\/tropej\/fmn038","article-title":"Relationship between Intestinal Parasitic Infection in Children and Soil Contamination in an Urban Slum","volume":"55","author":"F Korkes","year":"2007","journal-title":"Journal of Tropical Pediatrics"},{"key":"pntd.0008815.ref030","doi-asserted-by":"crossref","first-page":"e0157780","DOI":"10.1371\/journal.pone.0157780","article-title":"Soil-Transmitted Helminth Eggs Are Present in Soil at Multiple Locations within Households in Rural Kenya. Aroian RV, editor","volume":"11","author":"L Steinbaum","year":"2016","journal-title":"PLOS ONE"},{"key":"pntd.0008815.ref031","first-page":"6","article-title":"Prevalence of geohelminths ova in soils of selected public primary school premises in Benin city, south-south, Nigeria","volume":"17","author":"EU Edosomwan","year":"2017","journal-title":"NISEB Journal"},{"key":"pntd.0008815.ref032","doi-asserted-by":"crossref","first-page":"e003476","DOI":"10.1136\/bmjopen-2013-003476","article-title":"Cluster-randomised controlled trials of individual and combined water, sanitation, hygiene and nutritional interventions in rural Bangladesh and Kenya: the WASH Benefits study design and rationale","volume":"3","author":"BF Arnold","year":"2013","journal-title":"BMJ open"},{"key":"pntd.0008815.ref033","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.actatropica.2013.12.010","article-title":"School-based mass distributions of mebendazole to control soil-transmitted helminthiasis in the Munshiganj and Lakshmipur districts of Bangladesh: An evaluation of the treatment monitoring process and knowledge, attitudes, and practices of the population","volume":"141","author":"I Hafiz","year":"2015","journal-title":"Acta Tropica"},{"key":"pntd.0008815.ref034","doi-asserted-by":"crossref","DOI":"10.1186\/s13063-018-2710-8","article-title":"Achieving optimal technology and behavioral uptake of single and combined interventions of water, sanitation hygiene and nutrition, in an efficacy trial (WASH benefits) in rural Bangladesh","volume":"19","author":"SM Parvez","year":"2018","journal-title":"Trials."},{"key":"pntd.0008815.ref035","doi-asserted-by":"crossref","first-page":"e0195218","DOI":"10.1371\/journal.pone.0195218","article-title":"Unsafe disposal of feces of children <3 years among households with latrine access in rural Bangladesh: Association with household characteristics, fly presence and child diarrhea. Dearden KA, editor","volume":"13","author":"M Islam","year":"2018","journal-title":"PLoS ONE"},{"key":"pntd.0008815.ref036","doi-asserted-by":"crossref","first-page":"e302","DOI":"10.1016\/S2214-109X(17)30490-4","article-title":"Effects of water quality, sanitation, handwashing, and nutritional interventions on diarrhoea and child growth in rural Bangladesh: a cluster randomised controlled trial","volume":"6","author":"SP Luby","year":"2018","journal-title":"The Lancet Global Health"},{"key":"pntd.0008815.ref037","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1186\/s13063-018-2709-1","article-title":"WASH Benefits Bangladesh trial: management structure for achieving high coverage in an efficacy trial","volume":"19","author":"L Unicomb","year":"2018","journal-title":"Trials"},{"key":"pntd.0008815.ref038","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1186\/s13063-018-2708-2","article-title":"WASH Benefits Bangladesh trial: system for monitoring coverage and quality in an efficacy trial","volume":"19","author":"M Rahman","year":"2018","journal-title":"Trials"},{"key":"pntd.0008815.ref039","unstructured":"World Bank Group. Bangladesh. Climate Change Knowledge Portal. Available: https:\/\/climateknowledgeportal.worldbank.org\/country\/bangladesh\/climate-data-historical"},{"key":"pntd.0008815.ref040","volume-title":"Control of pathogens and vector attraction in sewage sludge..:","author":"U.S. Environmental Protection Agency","year":"2003"},{"key":"pntd.0008815.ref041","doi-asserted-by":"crossref","first-page":"e0005522","DOI":"10.1371\/journal.pntd.0005522","article-title":"Detecting and enumerating soil-transmitted helminth eggs in soil: New method development and results from field testing in Kenya and Bangladesh. 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Statistical Applications in Genetics and Molecular Biology"},{"key":"pntd.0008815.ref045","doi-asserted-by":"crossref","DOI":"10.18637\/jss.v051.i13","article-title":"tmle: An R Package for Targeted Maximum Likelihood Estimation","volume":"51","author":"S Gruber","year":"2012","journal-title":"Journal of Statistical Software"},{"key":"pntd.0008815.ref046","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.1002\/sim.1296","article-title":"Subgroup analysis, covariate adjustment and baseline comparisons in clinical trial reporting: current practiceand problems","volume":"21","author":"SJ Pocock","year":"2002","journal-title":"Statistics in Medicine"},{"key":"pntd.0008815.ref047","unstructured":"Islam M. Pit latrine emptying in rural Bangladesh. in preparation."},{"key":"pntd.0008815.ref048","doi-asserted-by":"crossref","first-page":"8725","DOI":"10.1021\/acs.est.7b01710","article-title":"Animal feces contribute to domestic fecal contamination: evidence from E. coli measured in water, hands, food, flies, and soil in Bangladesh.","volume":"51","author":"A Ercumen","year":"2017","journal-title":"Environmental Science & Technology"},{"key":"pntd.0008815.ref049","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1756-3305-5-42","article-title":"Are Ascaris lumbricoides and Ascaris suum a single species?","volume":"5","author":"D Leles","year":"2012","journal-title":"Parasit Vectors."},{"key":"pntd.0008815.ref050","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1017\/S0031182001008435","article-title":"Chickens and pigs as transport hosts for Ascaris, Trichuris and Oesophagostomum eggs","volume":"123","author":"A Olsen","year":"2001","journal-title":"Parasitology"},{"key":"pntd.0008815.ref051","unstructured":"Oswald WE, Halliday KE, Mcharo C, Witek-McManus S, Kepha S, Gichuki PM, et al. Domains of transmission and association of community, school, and household sanitation with soil-transmitted helminth infections among children in coastal Kenya.: 17."},{"key":"pntd.0008815.ref052","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.trstmh.2008.08.012","article-title":"High latrine coverage is not reducing the prevalence of soil-transmitted helminthiasis in Hoa Binh province, Vietnam","volume":"103","author":"A Yajima","year":"2009","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"pntd.0008815.ref053","article-title":"Effect of sanitation improvements on pathogens and microbial source tracking markers in the rural Bangladeshi household environment","author":"ER Fuhrmeister","year":"2020","journal-title":"Environ Sci Technol"},{"key":"pntd.0008815.ref054","doi-asserted-by":"crossref","first-page":"12089","DOI":"10.1021\/acs.est.8b02988","article-title":"Do sanitation improvements reduce fecal contamination of water, hands, food, soil, and flies? 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