{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:32:41Z","timestamp":1786357961343,"version":"build-2736575974"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Ascaris<\/jats:italic> is a soil-transmitted nematode that causes ascariasis, a neglected tropical disease affecting predominantly children and adolescents in the tropics and subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Exploring the molecular biology of <jats:italic>Ascaris<\/jats:italic> is important to gain a better understanding of the host-parasite interactions and disease processes, and supports the development of novel interventions. Although advances have been made in the genomics, transcriptomics and proteomics of <jats:italic>Ascaris<\/jats:italic>, its lipidome has received very limited attention. Lipidomics is an important sub-discipline of systems biology, focused on exploring lipids profiles in tissues and cells, and elucidating their biological and metabolic roles. Here, we characterised the lipidomes of key developmental stages and organ systems of <jats:italic>Ascaris<\/jats:italic> of porcine origin via high throughput LC-MS\/MS. In total, &gt; 500 lipid species belonging to 18 lipid classes within three lipid categories were identified and quantified\u2013in precise molar amounts in relation to the dry weight of worm material\u2013in different developmental stages\/sexes and organ systems. The results showed substantial differences in the composition and abundance of lipids with key roles in cellular processes and functions (e.g. energy storage regulation and membrane structure) among distinct stages and among organ systems, likely reflecting differing demands for lipids, depending on stage of growth and development as well as the need to adapt to constantly changing environments within and outside of the host animal. This work provides the first step toward understanding the biology of lipids in <jats:italic>Ascaris<\/jats:italic>, with possibilities to work toward designing new interventions against ascariasis.<\/jats:p>","DOI":"10.1371\/journal.pntd.0008848","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T18:26:19Z","timestamp":1606933579000},"page":"e0008848","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Quantitative lipidomic analysis of Ascaris 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