{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T06:45:56Z","timestamp":1793169956445,"version":"4.5.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T00:00:00Z","timestamp":1609200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902105"],"award-info":[{"award-number":["81902105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372446"],"award-info":[{"award-number":["31372446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Academician Yunde Hou Youth Foundation","award":["2019HYDQNJJ08"],"award-info":[{"award-number":["2019HYDQNJJ08"]}]},{"name":"Hebei key research and development projects","award":["19226628D"],"award-info":[{"award-number":["19226628D"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Brucella<\/jats:italic> spp. are facultative intracellular pathogens that can persistently colonize host cells and cause the zoonosis- brucellosis. The WHO recommended a treatment for brucellosis that involves a combination of doxycycline, rifampicin, or streptomycin. The aim of this study was to screen rifampicin-resistance related genes by transcriptomic analysis and gene recombination method at low rifampicin concentrations and to predict the major rifampicin- resistance pathways in <jats:italic>Brucella<\/jats:italic> spp. The results showed that the MIC value of rifampicin for <jats:italic>B<\/jats:italic>. <jats:italic>melitensis<\/jats:italic> bv.3 Ether was 0.5 \u03bcg \/ mL. Meanwhile, <jats:italic>B<\/jats:italic>. <jats:italic>melitensis<\/jats:italic> had an adaptive response to the resistance of low rifampicin in the early stages of growth, while the SNPs changed in the <jats:italic>rpoB<\/jats:italic> gene in the late stages of growth when incubated at 37\u00b0C with shaking. The transcriptome results of rifampicin induction showed that the functions of significant differentially expressed genes were focused on metabolic process, catalytic activity and membrane and membrane part. The <jats:italic>VirB<\/jats:italic> operon, \u03b2-resistance genes, ABC transporters, quorum-sensing genes, DNA repair- and replication -related genes were associated with rifampicin resistance when no variations of the in <jats:italic>rpoB<\/jats:italic> were detected. Among the <jats:italic>VirB<\/jats:italic> operons, VirB7-11 may play a central role in rifampicin resistance. This study provided new insights for screening rifampicin resistance-related genes and also provided basic data for the prevention and control of rifampicin-resistant <jats:italic>Brucella<\/jats:italic> isolates.<\/jats:p>","DOI":"10.1371\/journal.pntd.0008888","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T18:25:57Z","timestamp":1609266357000},"page":"e0008888","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cell membrane components of Brucella melitensis play important roles in the resistance of low-level rifampicin"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-9085","authenticated-orcid":true,"given":"Xiaowen","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonglei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guozhong","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongri","family":"Piao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,29]]},"reference":[{"issue":"11","key":"pntd.0008888.ref001","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1093\/infdis\/jiu673","article-title":"Brucella Intracellular Life Relies on the Transmembrane Protein CD98 Heavy Chain","volume":"211","author":"A Keriel","year":"2015","journal-title":"J Infect Dis"},{"issue":"Pt 11","key":"pntd.0008888.ref002","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.1099\/ijs.0.65269-0","article-title":"Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts","volume":"57","author":"G Foster","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"pntd.0008888.ref003","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1186\/s12917-015-0456-z","article-title":"First isolation and characterization of Brucella microti from wild boar","volume":"11","author":"Z Ronai","year":"2015","journal-title":"BMC Vet Res"},{"issue":"Pt 12","key":"pntd.0008888.ref004","doi-asserted-by":"crossref","first-page":"4120","DOI":"10.1099\/ijs.0.065482-0","article-title":"Brucella papionis sp. nov., isolated from baboons (Papio spp.)","volume":"64","author":"AM Whatmore","year":"2014","journal-title":"Int J Syst Evol Microbiol"},{"issue":"Pt 4","key":"pntd.0008888.ref005","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1099\/ijs.0.011148-0","article-title":"Brucella inopinata sp. nov., isolated from a breast implant infection","volume":"60","author":"HC Scholz","year":"2010","journal-title":"Int J Syst Evol Microbiol"},{"issue":"5","key":"pntd.0008888.ref006","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1099\/ijsem.0.000998","article-title":"Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)","volume":"66","author":"HC Scholz","year":"2016","journal-title":"Int J Syst Evol Microbiol"},{"issue":"1","key":"pntd.0008888.ref007","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis","volume":"4","author":"ML Boschiroli","year":"2001","journal-title":"Curr Opin Microbiol"},{"issue":"4","key":"pntd.0008888.ref008","doi-asserted-by":"crossref","first-page":"336","DOI":"10.15537\/smj.2018.4.21896","article-title":"Pediatric brucellosis. 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