{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:36:36Z","timestamp":1782488196996,"version":"3.54.5"},"reference-count":19,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"\n Background<\/jats:title>\n \n Eumycetoma is a neglected tropical disease most commonly caused by the fungus\n Madurella mycetomatis<\/jats:italic>\n . Identification of eumycetoma causative agents can only be reliably performed by molecular identification, most commonly by species-specific PCR. The current\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n specific PCR primers were recently discovered to cross-react with\n Madurella pseudomycetomatis<\/jats:italic>\n . Here, we used a comparative genome approach to develop a new\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n specific PCR for species identification.\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n Predicted-protein coding sequences unique to\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n were first identified in BLASTCLUST based on E-value, size and presence of orthologues. Primers were then developed for 16 unique sequences and evaluated against 60\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n isolates and other eumycetoma causing agents including the\n Madurella<\/jats:italic>\n sibling species. Out of the 16, only one was found to be specific to\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n We have discovered a predicted-protein coding sequence unique to\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n and have developed a new species-specific PCR to be used as a novel diagnostic marker for\n M<\/jats:italic>\n .\n mycetomatis<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pntd.0008897","type":"journal-article","created":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T15:11:26Z","timestamp":1608131486000},"page":"e0008897","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["The development of a novel diagnostic PCR for Madurella mycetomatis using a comparative genome approach"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7883-3825","authenticated-orcid":true,"given":"Wilson","family":"Lim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6314-7374","authenticated-orcid":true,"given":"Emmanuel","family":"Siddig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Eadie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1454-6149","authenticated-orcid":true,"given":"Bertrand","family":"Nyuykonge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Fahal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelies","family":"Verbon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5239-5321","authenticated-orcid":true,"given":"Sandra","family":"Smit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8652-8783","authenticated-orcid":true,"given":"Wendy WJ","family":"van de Sande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,16]]},"reference":[{"issue":"1","key":"pntd.0008897.ref001","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S1473-3099(15)00359-X","article-title":"Mycetoma: a unique neglected tropical disease","volume":"16","author":"EE Zijlstra","year":"2016","journal-title":"The Lancet Infectious Diseases"},{"issue":"11","key":"pntd.0008897.ref002","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1002\/bjs.1800791107","volume":"79","author":"AH Fahal","year":"1992","journal-title":"Mycetoma. 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