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Bats are important natural reservoir hosts of various lyssaviruses that can be transmitted to people. The epidemiology and pathogenesis of rabies in bats are poorly understood, making it difficult to prevent zoonotic transmission. To further our understanding of lyssavirus pathogenesis in a natural bat host, an experimental model using straw-colored fruit bats (<jats:italic>Eidolon helvum<\/jats:italic>) and Lagos bat virus, an endemic lyssavirus in this species, was developed. To determine the lowest viral dose resulting in 100% productive infection, bats in five groups (four bats per group) were inoculated intramuscularly with one of five doses, ranging from 10<jats:sup>0.1<\/jats:sup> to 10<jats:sup>4.1<\/jats:sup> median tissue culture infectious dose (TCID<jats:sub>50<\/jats:sub>). More bats died due to the development of rabies after the middle dose (10<jats:sup>2.1<\/jats:sup> TCID<jats:sub>50<\/jats:sub>, 4\/4 bats) than after lower (10<jats:sup>1.1<\/jats:sup>, 2\/4; 10<jats:sup>1.1<\/jats:sup>, 2\/4) or higher (10<jats:sup>3.1<\/jats:sup>, 2\/4; 10<jats:sup>4.1<\/jats:sup>, 2\/4) doses of virus. In the two highest dose groups, 4\/8 bats developed rabies. Of those bats that remained healthy 3\/4 bats seroconverted, suggesting that high antigen loads can trigger a strong immune response that abrogates a productive infection. In contrast, in the two lowest dose groups, 3\/8 bats developed rabies, 1\/8 remained healthy and seroconverted and 4\/8 bats remained healthy and did not seroconvert, suggesting these doses are too low to reliably induce infection. The main lesion in all clinically affected bats was meningoencephalitis associated with lyssavirus-positive neurons. Lyssavirus antigen was detected in tongue epithelium (5\/11 infected bats) rather than in salivary gland epithelium (0\/11), suggesting viral excretion via the tongue. Thus, intramuscular inoculation of 10<jats:sup>2.1<\/jats:sup> TCID<jats:sub>50<\/jats:sub> of Lagos bat virus into straw-colored fruit bats is a suitable model for lyssavirus associated bat rabies in a natural reservoir host, and can help with the investigation of lyssavirus infection dynamics in bats.<\/jats:p>","DOI":"10.1371\/journal.pntd.0008898","type":"journal-article","created":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T19:53:41Z","timestamp":1608062021000},"page":"e0008898","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Experimental Lagos bat virus infection in straw-colored fruit bats: A suitable model for bat rabies in a natural reservoir species"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8856-9135","authenticated-orcid":true,"given":"Lineke","family":"Begeman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Suu-Ire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1286-9825","authenticated-orcid":true,"given":"Ashley C.","family":"Banyard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Drosten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Eggerbauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1076-398X","authenticated-orcid":true,"given":"Conrad M.","family":"Freuling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7293-1475","authenticated-orcid":true,"given":"Louise","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hooman","family":"Goharriz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel L.","family":"Horton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4773-7977","authenticated-orcid":true,"given":"Daisy","family":"Jennings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9215-088X","authenticated-orcid":true,"given":"Denise A.","family":"Marston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3915-3341","authenticated-orcid":true,"given":"Yaa","family":"Ntiamoa-Baidu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9775-0550","authenticated-orcid":true,"given":"Silke","family":"Riesle Sbarbaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7529-7844","authenticated-orcid":true,"given":"David","family":"Selden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0279-3900","authenticated-orcid":true,"given":"Emma L.","family":"Wise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thijs","family":"Kuiken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3243-6154","authenticated-orcid":true,"given":"Anthony R.","family":"Fooks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L. 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colonies exposed to EBLV-1 (European bat lyssavirus type 1): Assessment of survival and serological status variations using capture-recapture models","volume":"11","author":"E Robardet","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0008898.ref024","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1017\/S0950268805003705","article-title":"Salivary excretion of rabies virus by healthy vampire bats","volume":"133","author":"\u00c1 Aguilar-Seti\u00e9n","year":"2005","journal-title":"Epidemiol Infect"},{"key":"pntd.0008898.ref025","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1017\/S0950268804003656","article-title":"Experimental rabies infection in haematophagous bats Desmodus rotundus","volume":"133","author":"MF Almeida","year":"2005","journal-title":"Epidemiol Infect"},{"key":"pntd.0008898.ref026","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1093\/infdis\/117.1.82","article-title":"Experimental rabies infection in the Mexican freetail bat","volume":"1","author":"GM Baer","year":"1967","journal-title":"J Infect Dis"},{"key":"pntd.0008898.ref027","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0064808","article-title":"Rabies virus infection in Eptesicus fuscus bats born in captivity (na\u00efve bats)","volume":"8","author":"AD Davis","year":"2013","journal-title":"PLoS ONE"},{"key":"pntd.0008898.ref028","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/s00705-012-1551-0","article-title":"Unique characteristics of bat rabies viruses in big brown bats (Eptesicus fuscus)","volume":"158","author":"AD Davis","year":"2013","journal-title":"Arch Virol"},{"key":"pntd.0008898.ref029","doi-asserted-by":"crossref","first-page":"9008","DOI":"10.1128\/JVI.03554-12","article-title":"Susceptibility and pathogenesis of little brown bats (Myotis lucifugus) to heterologous and homologous rabies viruses","volume":"87","author":"AD Davis","year":"2013","journal-title":"J 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Freuling","year":"2009","journal-title":"J Gen Virol"},{"key":"pntd.0008898.ref033","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1007\/s00705-005-0785-0","article-title":"Experimental infection of big brown bats (Eptesicus fuscus) with Eurasian bat lyssaviruses Aravan, Khujand, and Irkut virus","volume":"151","author":"GJ Hughes","year":"2006","journal-title":"Arch Virol"},{"key":"pntd.0008898.ref034","doi-asserted-by":"crossref","first-page":"612","DOI":"10.7589\/0090-3558-44.3.612","article-title":"Experimental rabies virus infection of big brown bats (Eptesicus fuscus)","volume":"44","author":"FR Jackson","year":"2008","journal-title":"J Wildl Dis"},{"key":"pntd.0008898.ref035","doi-asserted-by":"crossref","first-page":"2662","DOI":"10.1099\/vir.0.2008\/003889-0","article-title":"Experimental study of European bat lyssavirus type-2 infection in Daubenton's bats (Myotis daubentonii)","volume":"89","author":"N Johnson","year":"2008","journal-title":"J Gen 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with special reference to the clinical course and the latency of infection","volume":"30","author":"JL Pawan","year":"1936","journal-title":"Annals of Tropical Medicine & Parasitology"},{"key":"pntd.0008898.ref040","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/infdis\/112.1.17","article-title":"Studies on the pathogenesis of rabies in insectivorous bats. III. Influence of the gravid state","volume":"112","author":"RA Sims","year":"1963","journal-title":"J Infect Dis"},{"key":"pntd.0008898.ref041","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1093\/infdis\/98.1.10","article-title":"Experimental rabies infection in insectivorous bats","volume":"98","author":"DD Stamm","year":"1956","journal-title":"J Infect Dis"},{"key":"pntd.0008898.ref042","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1084\/jem.110.3.369","article-title":"Studies on the pathogenesis of rabies in insectivorous bats. I. Role of brown adipose tissue","volume":"110","author":"SE Sulkin","year":"1959","journal-title":"J Exp Med"},{"key":"pntd.0008898.ref043","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1084\/jem.112.4.595","article-title":"Studies on the pathogenesis of rabies in insectivorous bats: II. Influence of environmental temperature","volume":"112","author":"SE Sulkin","year":"1960","journal-title":"J Exp Med"},{"key":"pntd.0008898.ref044","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1007\/s007050070066","article-title":"Characterisation of a recently isolated lyssavirus in frugivorous zoo bats","volume":"145","author":"WH Van der Poel","year":"2000","journal-title":"Arch Virol"},{"key":"pntd.0008898.ref045","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1099\/vir.0.020073-0","article-title":"Host immunity to repeated rabies virus infection in big brown bats","volume":"91","author":"AS Turmelle","year":"2010","journal-title":"J Gen Virol"},{"key":"pntd.0008898.ref046","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cimid.2017.05.008","article-title":"Experimental infection of Artibeus intermedius with a vampire bat rabies virus","volume":"52","author":"C Obreg\u00f3n-Morales","year":"2017","journal-title":"Comp Immunol Microbiol 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tissues and molecular characterization of strains from naturally infected non-hematophagous bats","volume":"165","author":"SD Allendorf","year":"2012","journal-title":"Virus Res"},{"issue":"11","key":"pntd.0008898.ref051","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1007\/s00705-009-0504-8","article-title":"Repeated detection of European bat lyssavirus type 2 in dead bats found at a single roost site in the UK","volume":"154","author":"AC Banyard","year":"2009","journal-title":"Arch Virol"},{"key":"pntd.0008898.ref052","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1111\/j.1751-0813.1999.tb13198.x","article-title":"Histopathology and immunohistochemistry of bats infected by Australian bat lyssavirus","volume":"77","author":"PT Hooper","year":"1999","journal-title":"Aust Vet J"},{"issue":"12","key":"pntd.0008898.ref053","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1017\/S0950268812000167","article-title":"Endemic Lagos bat virus infection in 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